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See detailCharacterization of preferential activity on platelet thromboxane A2 receptors of BM-613, a new thromboxane A2 antagonist
Hanson, Julien ULg; Rolin, S.; De Leval, X. et al

in Fundamental & Clinical Pharmacology (2004)

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See detailCharacterization of pregnancy-associated glycoproteins extracted from zebu (Bos indicus) placentas removed at different gestational periods
Melo de Sousa, Noelita ULg; Remy, Benoit; El Amiri, Bouchra et al

in Reproduction Nutrition Development (2002), 42(3), 1-15

In the present work, two biochemical approaches were used to characterize PAGs isolated from Bos indicus fetal cotyledons removed at different gestational ages. The first procedure included acidic and ... [more ▼]

In the present work, two biochemical approaches were used to characterize PAGs isolated from Bos indicus fetal cotyledons removed at different gestational ages. The first procedure included acidic and ammonium sulfate precipitations, anion and cation exchange chromatographies and the second included pepstatin-agarose affinity chromatography. A bovine PAG radioimmunoassay was used to monitor the immunoreactivity throughout the isolation procedures. The most immunoreactive fractions issued from cation exchange and affinity chromatographies were analyzed by SDS-PAGE and Western blotting, before transfer to a polyvinylidene difluoride (PVDF) membrane for NH2-microsequence determination. Use SDS-PAGE and Western blotting, different isoforms of PAG with apparent molecular masses of 51 to 69 kDa and isoelectric points varying from 4.4 to 6.7 were identified in the placentas from different gestational ages. N-terminal microsequencing (10 to 25 aa long) indicates the expression of one single terminal amino acid sequence in the Bos indicus placenta, which is 100% identical to the bovine PAG-1. [less ▲]

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See detailCharacterization of printed circuit boards from scrap printers
Silvas, Flávia; de Moraes, Viviane; Bortolini, Guilherme et al

in Yurko, James; Zhang, Lifeng; Allanore, Antoine (Eds.) Proceedings TMS 2014 143rd ANNUAL MEETING & EXHIBITION (2014, April)

Printed circuit boards (PCB) are particularly problematic to recycle because of the heterogeneous mix of organic materials, metals, and glass fiber. PCBs are found in almost every electroelectronic ... [more ▼]

Printed circuit boards (PCB) are particularly problematic to recycle because of the heterogeneous mix of organic materials, metals, and glass fiber. PCBs are found in almost every electroelectronic equipment and their recycling process involves a pre-treatment to separate the products which contain targeted metals. In this work, a physical processing involving sequential shredding and hammer milling were used to obtain a comminuted sample from scrap printers PCBs. The thus prepared sample was analyzed for particle size distribution and by optical microscopy as well was subjected to pyrolysis, leachabilty and chemical composition determination by XRF. The metals concentration was determined by ICP and AAS. The PCBs have been also analyzed by SEM-EDS (micro-analysis and X-ray mapping). The results show that the studied PCBs are lead free, multilayer with 4 copper layers, have 45% of metals and the copper is present in significantly higher concentration than the rest of the metals. [less ▲]

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See detailCharacterization of prolatinomas resistant to dopaminergic agonists
Vroonen, Laurent ULg; Tamagno, G.; Naves, L. et al

in 18th Meeting of the Belgian Endocrine and Metabolic societies : Bruxelles, 25 octobre 2008 (2008, October)

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See detailCharacterization of prolatinomas resistant to dopaminergic agonists
Vroonen, Laurent ULg; Daly, Adrian ULg; Beckers, Albert ULg

in ENDO 2010 : The 92nd Annual Meeting (2010, June)

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See detailCharacterization of prolatinomas resistant to dopaminergic agonists
Vroonen, Laurent ULg; Tamagno, G.; Naves, L. et al

in 19 Meeting of the Belgian Endocrine and Metabolic societies (2009)

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See detailCHARACTERIZATION OF PROTEINURIA IN DOGUE DE BORDEAUX DOGS USING ELECTROPHORESIS AND URINARY BIOMARKERS
Lavoué, Rachel; Smets, P.; Trumel, Cathy et al

in Proceedings of the 2014 ACVIM Forum (2014, June)

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailCharacterization of recombinant wild type and site-directed mutations of apolipoprotein C-III: lipid binding, displacement of ApoE, and inhibition of lipoprotein lipase.
Liu, H.; Talmud, P. J.; Lins, Laurence ULg et al

in Biochemistry (2000), 39(31), 9201-12

The physicochemical properties of recombinant wild type and three site-directed mutants of apolipoprotein C-III (apoC-III), designed by molecular modeling to alter specific amino acid residues implicated ... [more ▼]

The physicochemical properties of recombinant wild type and three site-directed mutants of apolipoprotein C-III (apoC-III), designed by molecular modeling to alter specific amino acid residues implicated in lipid binding (L9T/T20L, F64A/W65A) or LPL inhibition (K21A), were compared. Relative lipid binding efficiencies to dimyristoylphosphatidylcholine (DMPC) were L9T/T20L > WT >K21A > F64A/W65A with an inverse correlation with size of the discoidal complexes formed. Physicochemical analysis (Trp fluorescence, circular dichroism, and GdnHCl denaturation) suggests that L9T/T20L forms tighter and more stable lipid complexes with phospholipids, while F64A/W65A associates less tightly. Lipid displacement properties were tested by gel-filtrating apoE:dipalmitoylphosphatidylcholine (DPPC) discoidal complexes mixed with the various apoC-III variants. All apoC-III proteins bound to the apoE:DPPC complexes; the amount of apoE displaced from the complex was dependent on the apoC-III lipid binding affinity. All apoC-III proteins inhibited LPL in the presence or absence of apoC-II, with F64A/W65A displaying the most inhibition, suggesting that apoC-III inhibition of LPL is independent of lipid binding and therefore of apoC-II displacement. Taken together. these data suggest that the hydrophobic residues F64 and W65 are crucial for the lipid binding properties of apoC-III and that redistribution of the N-terminal helix of apoC-III (L9T/T20L) enhances the stability of the lipid-bound protein, while LPL inhibition by apoC-III is likely to be due to protein:protein interactions. [less ▲]

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See detailCharacterization of regulatory elements in the medaka osterix promoter required for osteoblast expression.
Renn, Jörg ULg; Buettner, A; Chua, EPS et al

in Journal of Applied Ichthyology (2014), 30(4), 652660

The zinc finger transcription factor Osterix/Sp7 is an essential regulator of osteoblastogenesis. In mammals, osterix expression is regulated by Runx2, Msx2 and Dlx5 but recent findings suggest that also ... [more ▼]

The zinc finger transcription factor Osterix/Sp7 is an essential regulator of osteoblastogenesis. In mammals, osterix expression is regulated by Runx2, Msx2 and Dlx5 but recent findings suggest that also retinoic acid plays an important role for osteoblast differentiation and function. Yet, how these and other factors act on the osterix promoter is largely unknown. Expression, knock-down and promoter analyses have indicated that the function of Osterix in osteoblasts is conserved in teleosts and mammals. In the present study, we have used the teleost medaka to identify and characterize a region containing potential retinoic acid response elements in the osterix promoter. We analysed whether this region is important for activity in osteoblasts in vivo, using transgenic medaka lines with modified osterix promoter regions. Promoter activity in vivo and in vitro revealed a short nucleotide sequence in the promoter with crucial positive regulatory function. Mutations of this element lead to a complete inactivation of the osterix promoter in osteoblasts and made it insensitive to retinoic acid treatment. The comparison with the regulatory regions of osterix in other species suggests that the function of this element is highly conserved in vertebrates. [less ▲]

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See detailCharacterization of regulatory functions of the varicella-zoster virus gene-63-encoded protein
Jackers, Pascale ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Journal of Virology (1992), 66(6), 3899-3903

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex ... [more ▼]

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex virus type 1. ICP22 is a polypeptide synthesized in herpes simplex virus type 1-infected cells, and as is the case for its VZV counterpart, its regulatory functions are unknown. On the basis of the VZV DNA sequence, it has been shown that IE63 exhibits hydrophilic and acidic properties, suggesting that this protein could play a regulatory role during the infectious cycle. We report in this article cotransfection experiments which demonstrate that the VZV gene 63 protein strongly represses, in a dose-dependent manner, the expression of VZV gene 62. On the other hand, transient expression of the VZV gene 63 protein can promote activation of the thymidine kinase gene but cannot affect the expression of the genes encoding glycoproteins I and II. The results of transient expression experiments strongly suggest that the VZV gene 63 protein could play a pivotal role in the repression of IE gene expression as well as in the activation of early gene expression [less ▲]

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See detailCharacterization of riparian zones in wallonia (belgium)
Michez, Adrien ULg; Piégay, Hervé; Lejeune, Philippe et al

Conference (2014, June 16)

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to ... [more ▼]

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to regional scale (ca. 13000 km for 1000 management sectors). The aim of this remotely sensed monitoring is to improve the river and riparian zone management and planning by providing some key information for river managers. This first research is exclusively based on data which are available at the regional scale (170000 square km) to develop automated tools to implement the methodology to the whole Walloon river network (13000 km) before 2015 with an update frequency of 3 years (photogrammetric DSM survey frequency). [less ▲]

Detailed reference viewed: 31 (10 ULg)