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See detailCharacterization of caprine herpesvirus 1 glycoprotein D gene and its translation product
Keuser, Véronique; Detry, Bruno; Thiry, Julien ULg et al

in Virus Research (2006), 115(2), 112-121

Caprine herpesvirus 1 (CpHV- 1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 ... [more ▼]

Caprine herpesvirus 1 (CpHV- 1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 (BoHV-1) which causes infectious bovine rhinotracheitis. Glycoprotein D (gD) mediates important functions in alphaherpesviruses and is also a main inummogen. The sequence of CpHV-1 gD gene and the biochemical properties of its translation product were analyzed and compared to those of BoHV-1 and other alphaherpesviruses. A relatively high homology was found between CpHV-1 and BoHV-1 glycoproteins D amino acid sequences (similarity of 68.8%). Moreover, six cysteine residues are conserved by CpHV-1 gD and the other studied alphaherpesviruses. CpHV-1 gD has a molecular mass similar to BoHV-1 gD and contains complex N-linked oligosaccharides. In contrast to the BoHV-1 gD, CpHV-1 gD is expressed as a late protein. In spite of the observed differences which could influence its biological functions, CpHV-1 gD, shares most characteristics with other alphaherpesviruses and especially BoHV-1. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailCharacterization of CD8+ T-cell response in acute and resolved hepatitis A virus infection.
Schulte, I.; Hitziger, T.; Giugliano, S. et al

in Journal of Hepatology (2011), 54(2), 201-208

Abstract BACKGROUND & AIMS: In contrast to the infection with other hepatotropic viruses, hepatitis A virus (HAV) always causes acute self-limited hepatitis, although the role for virus-specific CD8 T ... [more ▼]

Abstract BACKGROUND & AIMS: In contrast to the infection with other hepatotropic viruses, hepatitis A virus (HAV) always causes acute self-limited hepatitis, although the role for virus-specific CD8 T cells in viral containment is unclear. Herein, we analyzed the T cell response in patients with acute hepatitis by utilizing a set of overlapping peptides and predicted HLA-A2 binders from the polyprotein. METHODS: A set of 11 predicted peptides from the HAV polyprotein, identified as potential binders, were synthesized. Peripheral blood mononuclear cells (PBMCs) from patients were tested for IFNγ secretion after stimulation with these peptides and ex vivo with HLA-A2 tetramers. Phenotyping was carried out by staining with the activation marker CD38 and the memory marker CD127. RESULTS: Eight out of 11 predicted HLA-A2 binders showed a high binding affinity and five of them were recognized by CD8+ T cells from patients with hepatitis A. There were significant differences in the magnitude of the responses to these five peptides. One was reproducibly immunodominant and the only one detectable ex vivo by tetramer staining of CD8+ T cells. These cells have an activated phenotype (CD38hi CD127lo) during acute infection. Three additional epitopes were identified in HLA-A2 negative patients, most likely representing epitopes restricted by other HLA-class I-alleles (HLA-A11, B35, B40). CONCLUSIONS: Patients with acute hepatitis A have a strong multi-specific T cell response detected by ICS. With the tetramer carrying the dominant HLA-A2 epitope, HAV-specific and activated CD8+ T cells could be detected ex vivo. This first description of the HAV specific CTL-epitopes will allow future studies on strength, breadth, and kinetics of the T-cell response in hepatitis A. [less ▲]

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See detailCharacterization of changes in coronary blood flow during the first six seconds after intracoronary contrast injection.
Hodgson, J. M.; Mancini, G. B.; Legrand, Victor ULg et al

in Investigative radiology (1985), 20(3), 246-52

With current interest in digital and densitometric angiographic techniques for evaluating coronary blood flow (CBF), the immediate effects of intracoronary contrast injections on CBF are important to ... [more ▼]

With current interest in digital and densitometric angiographic techniques for evaluating coronary blood flow (CBF), the immediate effects of intracoronary contrast injections on CBF are important to establish. We investigated the effects of ECG-gated intracoronary injection of contrast on CBF with different injection rates (1-6 cc/sec), volumes (1/2-6 cc), and contrast concentrations (1/2, full strength). Sixteen open-chest dogs were instrumented with electromagnetic flow probes on the left anterior descending artery. During intracoronary injections of contrast, a characteristic decrease in coronary blood flow to approximately 45% of preinjection levels was seen an average of 1.9 seconds after intracoronary contrast injection. The timing of the depression in coronary flow was most influenced by the rate of contrast injection, and the magnitude of depression in flow was influenced by both the volume and concentration of the contrast injection. Angiographic techniques using the first 6 seconds after injection to obtain CBF measurements should take these characteristic changes into account to avoid possible errors. [less ▲]

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See detailCharacterization of chemotherapy-induced cell death in glioblastoma
Coupienne, Isabelle ULg; Fettweis, Grégory ULg; Piette, Jacques ULg

Poster (2012, December 10)

Glioblastoma constitute the most frequent and deadliest type of brain tumors. Their annual incidence is estimated between 5 and 8 cases per 100,000 people in Europe and North America. They are resistant ... [more ▼]

Glioblastoma constitute the most frequent and deadliest type of brain tumors. Their annual incidence is estimated between 5 and 8 cases per 100,000 people in Europe and North America. They are resistant to all current therapies and are associated with a very high rate of recurrence. The associated prognosis is generally very poor and most patients die within a year after diagnosis. Unfortunately, despite extensive research and use of multimodality treatments combining surgical resection, chemotherapy and radiotherapy, survival hasn’t really much improved over the last 20 years. Indeed, these tumors were shown to be characterized by a high radio- and chemo-resistance. Glioblastoma cells exhibit overexpression of the anti-apoptotic Bcl-2 family proteins and downregulation of its pro-apoptotic members, high expression of the IAPs (Inhibitors of Apoptosis Proteins) and constitutive activation of the pro-survival NF-κB pathway. Currently, the most commonly used treatment offering the best prognosis to patients consists in a combination of maximal surgical tumor resection (when feasible) with subsequent radio- and/or chemotherapy. Among the most commonly used chemotherapeutic agents, the alkylating agent temozolomide and the topoisomerase I inhibitor camptothecin occupy a central position. Therefore, in this study, the impact of both temozolomide and irinotecan (a soluble derivative of camptothecin) on glioma cell survival will be investigated. Important progress was made in the comprehension of the molecular mechanisms underlying tumor development and progression however, survival benefits conferred by the use of new drugs and therapeutic strategies are counted in months rather than years. Consequently, there is an urge to rapidly improve the efficiency of the currently used treatments. This research project consists in (i) the study of the mechanisms implicated in glioblastoma cell death induced by two chemotherapeutic agents : temozolomide and irinotecan, (ii) the identification of the mechanisms underlying the resistance of glioblastoma to these treatments, (iii) the use of pharmacological tools to interfere with those resistance strategies to enhance chemotherapy efficiency. Necrosis was, until recently, long thought to be only accidental. However, it was shown to be finely regulated by specific signalling pathways. Programmed necrosis often takes place in cells in which apoptosis cannot be properly activated and serves as a back-up cell death pathway. Previous work from our lab having already demonstrated that glioblastoma are, at least partially, apoptosis-defective, special emphasis is put on the study of necrotic parameters. Results from survival tests performed on several glioblastoma cell lines in the presence of necrotic inhibitors like necrostatin-1 (an inhibitor of the central regulator of the necrotic pathway RIP1) allows to partially overcome temozolomide and irinotecan-induced glioblastoma cell death, highlighting the role played by programmed necrosis in chemotherapy sensitivity. [less ▲]

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See detailCharacterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54, and Their Role in Bacterial Midrib Rot Disease of Lettuce
Huang, CJ; Pauwelyn, E.; Ongena, Marc ULg et al

in Molecular Plant-Microbe Interactions [=MPMI] (2015), 28(9), 109-1022

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See detailCharacterization of clay deposits of Nanga-Eboko (Central Cameroon): Suitability in the production of building materials
Mache, Jacques Richard ULg

in Clay Minerals (2013), 48

Clayey deposits of Nanga-Eboko (Central Cameroon) were studied to assess their potential as building materials. Characterization was performed using XRD, IR, XRF, DTA/DTG and firing. Clays appear as ... [more ▼]

Clayey deposits of Nanga-Eboko (Central Cameroon) were studied to assess their potential as building materials. Characterization was performed using XRD, IR, XRF, DTA/DTG and firing. Clays appear as discontinuous pockets with the same organizational characteristics in three villages located on both sides of the Sanaga River. The average thickness of exploitable layer is about 3m. The estimated tonnage ~7-17x105 m3 can supply an industry of great importance. SiO2 (~70%), Al2O3 (~15%) and Fe2O3 (~4%) are the predominant oxides of the natural clays. Quartz (~55%), kaolinite (~33%), illite (~5%) and K-feldspar (~4%) are major minerals. Clays are not suitable for building construction due to their fine-grained size and high plasticity properties. Firing properties of bricks (950 and 1050°C) are good despite the high shrinkage values. Therefore the addition of "degreasers' is recommended to control shaping and drying. [less ▲]

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See detailCharacterization of clay deposits of Nanga-Eboko (Central Cameroon): Suitability in the production of building materials
Nzeukou Nzeugang, A.; Medjo Eko, R.; Fagel, Nathalie ULg et al

in Clay Minerals (2013), 48(4), 655-662

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See detailCharacterization of clay from northern of Morocco for their industrial application
El Ouahabi, Meriam ULg; Fagel, Nathalie ULg

Poster (2010, May 04)

Clays are a natural resource used for millennia. Currently applications such as industrial minerals are diversified. In this context, our goal is to estimate the potential of the many clay deposits in ... [more ▼]

Clays are a natural resource used for millennia. Currently applications such as industrial minerals are diversified. In this context, our goal is to estimate the potential of the many clay deposits in northern of Morocco. The choice of this region is justified by the particular abundance of clay deposits used to manufacture building materials (brick, ceramic and refractories) and pottery. This study focuses on the mineralogical, chemical and geotechnical characterization tests carried out on Tangier-Tetouan and Meknes clays from northern of Morocco. The suitability of raw clay material from those regions in order to produce ceramic and brick has not been tested yet. The results revealed that the studied samples are diversified, kaolinite and illite (Tetouan clay) and kaolinite and illite and smectite and vermiculite (Tangier and Meknes clay) based materials. There were no major differences in grain-size distribution, whereas Meknes clay was more plastic than Tetouan-Tangier clay. The cation exchange capacity show that Meknes and Tangier clay were more important than Tetouan clay. Specific surface area and thermal analaysis complete this caracterization. It was found that almost all technological properties of the Meknes clay deposit are led to the manufacture of ceramic floor tile, and Tetouan-Tangier clay provide opportunities to making brick and ceramic floor. The Tetouan- Tangier and Meknes clay are a potential ceramic raw material for growing Morrocan ceramic tile and brick industries [less ▲]

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See detailCharacterization of Clostridium perfringens isolated from necrotic enteritis of fowls.
Das, B. C.; Dutta, G. N.; Daube, Georges ULg et al

in Indian Journal of Animal Sciences (The) (1997), 67

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See detailCharacterization of collagen fibrils after equine suspensory ligament injury: an ultrastructural and biochemical approach
Shikh Al Sook, Mohamad Khir ULg; Gabriel, Annick ULg; Salouci, Moustafa et al

in Veterinary Journal (2015)

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril ... [more ▼]

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril morphology, as well as the collagen content and types. The purpose of this study was to evaluate, using biochemical and ultrastructural approaches, the alterations in collagen fibrils after injury. Eight Warmblood horses with visible signs of injury in only one forelimb SL were selected and specimens were examined by transmission electron microscope (TEM). Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin. TEM revealed abnormal organization as well as alterations in the diameter and shape of fibrils after SL injury. The bands corresponding to types I, III and V collagen were assessed by densitometry after sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric analysis indicated that the proportions of type III and type V collagen were significantly higher (P <0.001) in damaged tissues compared to normal tissues with a mean increase of 20.9 and 17.3% respectively. Concurrently, a significant decrease (P <0.001) in type I collagen within damaged tissues was recorded with a mean decrease of 15.2%. These alterations could be the hallmark of a decrease in the tissue quality and mechanical properties of the ligament. This provides new insight for subsequent research on tissue regeneration that may lead to the development of future treatment strategies for SL injury. [less ▲]

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See detailCharacterization of collagen fibrils after equine suspensory ligament injury: an ultrastructural and biochemical approach
Shikh Al Sook, Mohamad Khir ULg; Gabriel, Annick ULg; Salouci, Moustafa et al

Poster (2014, October 19)

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril ... [more ▼]

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril morphology, as well as the collagen content and types. The purpose of this study was to evaluate, using biochemical and ultrastructural approaches, the alterations in collagen fibrils after injury. Eight Warmblood horses with visible signs of injury in only one forelimb SL were selected and specimens were examined by transmission electron microscope (TEM). Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin. TEM revealed abnormal organization as well as alterations in the diameter and shape of fibrils after SL injury. The bands corresponding to types I, III and V collagen were assessed by densitometry after sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric analysis indicated that the proportions of type III and type V collagen were significantly higher (P <0.001) in damaged tissues compared to normal tissues with a mean increase of 20.9 and 17.3% respectively. Concurrently, a significant decrease (P <0.001) in type I collagen within damaged tissues was recorded with a mean decrease of 15.2%. These alterations could be the hallmark of a decrease in the tissue quality and mechanical properties of the ligament. This provides new insight for subsequent research on tissue regeneration that may lead to the development of future treatment strategies for SL injury. [less ▲]

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See detailCharacterization of complex I mutants in Chlamydomonas reinhardtii : Role of structural subunits and identification of assembly factors.
Larosa, Véronique ULg; Barbieri, Rosario; Bonnefoy, Nathalie et al

Scientific conference (2009)

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See detailCharacterization of concrete surface roughness and its relation to adhesion in repair systems
Garbacz, A.; Courard, Luc ULg; Kostana, K.

in Materials Characterization (2006), 56(4-mai Sp. Iss. SI), 281-289

The aim of this work to analyse the relationships between parameters of concrete surface geometry determined with various methods as well as their relations to adhesion in repair systems. Several types of ... [more ▼]

The aim of this work to analyse the relationships between parameters of concrete surface geometry determined with various methods as well as their relations to adhesion in repair systems. Several types of concrete preparation techniques were selected to obtain different levels of surface roughness of concrete substrates. Four measurement techniques, corresponding to different levels of observation, have been used to characterize the surface geometry: laser profilometry, mechanical profilometry, a microscopic method and a "sand" (macroscopic) method. The correlations between parameters describing surface geometry are determined. The relationships between bond strength and selected parameters of surface geometry are also discussed. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailCharacterization of copper grain growth limitations inside narrow wires depending of overburden thickness
Dubreuil, Olivier ULg; Cordeau, Maryline; Mourier, Thierry et al

in Microelectronic Engineering (2008, October), 85(10), 1988-1991

With the downscaling of feature dimensions, copper interconnects exhibit properties differing from bulk or film material. Resistivity increases and limits electrical performances, and reliability of ... [more ▼]

With the downscaling of feature dimensions, copper interconnects exhibit properties differing from bulk or film material. Resistivity increases and limits electrical performances, and reliability of interconnects becomes a more important challenge for each new technological node. In this study, we present an approach of copper grain growth control inside narrow wires by adding a step between the copper electro-chemical deposition (ECD) and the chemical–mechanical polishing (CMP). This step corresponds to a partial CMP step (pre-CMP) and is applied after ECD and before anneal in order to modify the copper previous termoverburdennext term thickness. Depending on the targeted thickness, copper grain growth occurs during anneal with different efficiencies. Crystallization and grain growth behavior inside wires is investigated with focused ions beam (FIB). We present here our methodology for sample preparation and characterization. Results are focused on electrical variations and on morphological aspects of copper crystallization and grain growth inside lines observed with various overburden thicknesses. [less ▲]

Detailed reference viewed: 48 (1 ULg)