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See detailCharacterization of new fluorescent siderophores produced by Pseudomonas sp.
Jacques, Philippe ULg; Hautot, D.; Roblain, D. et al

Poster (1989, May)

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See detailCharacterization of non pigmented B16 melanoma cell-derived cytotoxic factors.
Siwek, B.; De Pauw-Gillet, Marie-Claire ULg; Quetin-Leclercq, J. et al

in Chemico-Biological Interactions (1997), 103(1), 59-73

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests ... [more ▼]

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests used showed that ultrafiltrated conditioned media (CM U1 fraction) contained several cytotoxic factors with a Mw lower than 1000 Da. These factors seemed to act either directly or indirectly on cell membranes, mitochondria, on the cell cycle and on protein and DNA synthesis. A cytotoxic activity could be found even after high dilution of CM U1. These cytotoxic factors were rapidly released by B16 cells in culture, independently of cell confluence. Their activities in the treated cells were also very fast and the cytotoxic effects were irreversible after only a few hours of treatment. These factors were not intermediate products during melanogenesis, neither polyamines, nor proteases. At least one of them seemed to be a small acidic and basic stable peptide without disulfide bounds but not heat stable. The synthesis of at least one of these cytotoxic factors was inhibited by cycloheximide and the cytotoxic activity was partially destroyed by pronase and trypsin, but not by pepsin. The cytotoxicity was not modified by copper complexants or free radical inhibitors (bovine serum albumin (BSA), tyrosine, superoxyde dismutase (SOD), catalase, vitamin E). Furthermore the levels of glutathione peroxydase activity and reduced glutathione did not change after treatment by CM U1 as compared to controls. [less ▲]

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See detailCharacterization of nonlinear aeroservoelastic behaviour
Dimitriadis, Grigorios ULg; Cooper, Jonathan Edware

in RTO-MP-036 Structural Aspects of Flexible Aircraft Control (1999, October)

The characterisation of the behaviour of nonlinear aeroelastic systems has become a very important re- search topic. Nevertheless, most of the work carried out to date concerns the development of unsteady ... [more ▼]

The characterisation of the behaviour of nonlinear aeroelastic systems has become a very important re- search topic. Nevertheless, most of the work carried out to date concerns the development of unsteady CFD solutions in the transonic region. Important though this work is, there is also a need for research which aims at understanding the behaviour of non- linear systems, particularly the occurance of Limit Cycle Oscillations (LCOs). The purpose of this pa- per is to study the stability of a simple aeroservoelas- tic system with nonlinearities in the control system. The work considers both structural and control law nonlinearities and assesses the stability of the system response by use of bifurcation diagrams. It is shown that simple feedback systems designed to increase the stability of the linearised system also stabilise the nonlinear system, although their effects can be less pronounced. Additionally, a nonlinear control law designed to limit the control surface pitch response was found to increase the flutter speed considerably by forcing the system to undergo limit cycle oscilla- tions instead of fluttering. Finally, friction was found to affect the damping of the system but not its sta- bility, as long as the amplitude of the frictional force is low enough not to cause stoppages in the motion. [less ▲]

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See detailCharacterization of olefins from enzymatically interesterified palm oil
Gibon, Véronique; Maes, Jeroen; Dijckmans, Peggy et al

Poster (2013)

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See detailCharacterization of Oligomeric Species on the Aggregation Pathway of Human Lysozyme
Frare, Erica; Mossuto, Maria F.; Polverino de Laureto, Patrizia et al

in Journal of Molecular Biology (2009)

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See detailCharacterization of Oligomeric Species on the Aggregation Pathway of Human Lysozyme
Frare, Erica; Mossuto, Maria F.; Polverino de Laureto, Patrizia et al

in Journal of Molecular Biology (2009), 387

has been analyzed by characterizing a series of distinct species formed on the aggregation pathway, specifically the amyloidogenic monomeric precursor protein, the oligomeric soluble prefibrillar ... [more ▼]

has been analyzed by characterizing a series of distinct species formed on the aggregation pathway, specifically the amyloidogenic monomeric precursor protein, the oligomeric soluble prefibrillar aggregates, and the mature fibrils. Particular attention has been focused on the analysis of the structural properties of the oligomeric species, since recent studies have shown that the oligomers formed by lysozyme prior to the appearance of mature amyloid fibrils are toxic to cells. Here, soluble oligomers of human lysozyme have been analyzed by a range of techniques including binding to fluorescent probes such as thioflavin T and 1-anilino-naphthalene-8-sulfonate, Fourier transforminfrared spectroscopy, and controlled proteolysis. Oligomers were isolated after 5 days of incubation of the protein and appear as spherical particles with a diameter of 8–17 nm when observed by transmission electron microscopy. Unlike the monomeric protein, oligomers have solventexposed hydrophobic patches able to bind the fluorescent probe 1-anilinonaphthalene- 8-sulfonate. Fourier transforminfrared spectroscopy spectra of oligomers are indicative of misfolded species when compared to monomeric lysozyme, with a prevalence of random structure but with significant elements of the β-sheet structure that is characteristic of the mature fibrils. Moreover, the oligomeric lysozyme aggregates were found to be more susceptible to proteolysis with pepsin than both the monomeric protein and the mature fibrils, indicating further their less organized structure. In summary, this study shows that the soluble lysozyme oligomers are locally unfolded species that are present at low concentration during the initial phases of aggregation. The nonnative conformational features of the lysozyme molecules of which they are composed are likely to be the factors that confer on them the ability to interact inappropriately with a variety of cellular components including membranes. [less ▲]

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See detailCharacterization of olive cake production in northern Morocco
El Otmani, Samira; Ayadi, Mohammed; Chentouf, Mouad et al

in 1st FARAH Day, Faculty of Veterinary Medicine (2014)

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See detailCharacterization of ovine herpesvirus 2-induced malignant catarrhal fever in rabbits
Li; Cunha, C.W.; Gailbreath, K.L. et al

in Veterinary Microbiology (2011), 2

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See detailCharacterization of ovine herpesvirus 2-induced malignant catarrhal fever in rabbits
Li, Hong; Cunha, Cristina W.; Gailbreath, Katherine L. et al

in Veterinary Microbiology (2011), 150(3-4), 270-7

Malignant catarrhal fever (MCF) is a frequently fatal lymphoproliferative disease syndrome primarily of ruminant species, caused by gammaherpesviruses in the genus Macavirus. Ovine herpesvirus 2 (OvHV-2 ... [more ▼]

Malignant catarrhal fever (MCF) is a frequently fatal lymphoproliferative disease syndrome primarily of ruminant species, caused by gammaherpesviruses in the genus Macavirus. Ovine herpesvirus 2 (OvHV-2), carried by sheep, causes sheep-associated MCF worldwide, while Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest, causes wildebeest-associated MCF, mainly in Africa. Diseases in rabbits can be induced by both viruses, which are clinically and pathologically similar; however, recent studies revealed different expression of viral genes associated with latency or lytic replication during clinical disease between the two viruses. In this study, we further characterized experimentally induced MCF in rabbits by nebulization with OvHV-2 from sheep nasal secretions to elucidate the course of viral replication, along with in vivo incorporation of 5-Bromo-2’-Deoxyuridine (BrdU), to evaluate lymphoproliferation. All six rabbits nebulized with OvHV-2 developed MCF between 24 and 29 days post infection. OvHV-2 DNA levels in peripheral blood leukocytes (PBL) remained undetectable during the incubation period and increased dramatically a few days before onset of clinical signs. During the clinical stage, we found that predominantly lytic gene expression was detected in PBL and tissues, and both T and B cells were proliferating. The data showed that the viral gene expression profile and lymphoproliferation in rabbits with OvHV-2 induced MCF were different from that in rabbits with AlHV-1 induced MCF, suggesting that OvHV-2 and AlHV-1 may play a different role in MCF pathogenesis. [less ▲]

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See detailCharacterization of OXA-29 from Legionella (Fluoribacter) gormanii: molecular class D beta-lactamase with unusual properties.
Franceschini, N.; Boschi, L.; Pollini, S. et al

in Antimicrobial agents and chemotherapy (2001), 45(12), 3509-16

A class D beta-lactamase determinant was isolated from the genome of Legionella (Fluoribacter) gormanii ATCC 33297(T). The enzyme, named OXA-29, is quite divergent from other class D beta-lactamases ... [more ▼]

A class D beta-lactamase determinant was isolated from the genome of Legionella (Fluoribacter) gormanii ATCC 33297(T). The enzyme, named OXA-29, is quite divergent from other class D beta-lactamases, being more similar (33 to 43% amino acid identity) to those of groups III (OXA-1) and IV (OXA-9, OXA-12, OXA-18, and OXA-22) than to other class D enzymes (21 to 24% sequence identity). Phylogenetic analysis confirmed the closer ancestry of OXA-29 with members of the former groups. The OXA-29 enzyme was purified from an Escherichia coli strain overexpressing the gene via a T7-based expression system by a single ion-exchange chromatography step on S-Sepharose. The mature enzyme consists of a 28.5-kDa polypeptide and exhibits an isoelectric pH of >9. Analysis of the kinetic parameters of OXA-29 revealed efficient activity (k(cat)/K(m) ratios of >10(5) M(-1) x s(-1)) for several penam compounds (oxacillin, methicillin, penicillin G, ampicillin, carbenicillin, and piperacillin) and also for cefazolin and nitrocefin. Oxyimino cephalosporins and aztreonam were also hydrolyzed, although less efficiently (k(cat)/K(m) ratios of around 10(3) M(-1) x s(-1)). Carbapenems were neither hydrolyzed nor inhibitory. OXA-29 was inhibited by BRL 42715 (50% inhibitory concentration [IC(50)], 0.44 microM) and by tazobactam (IC(50), 3.2 microM), but not by clavulanate. It was also unusually resistant to chloride ions (IC(50), >100 mM). Unlike OXA-10, OXA-29 was apparently found as a dimer both in diluted solutions and in the presence of EDTA. Its activity was either unaffected or inhibited by divalent cations. OXA-29 is a new class D beta-lactamase that exhibits some unusual properties likely reflecting original structural and mechanistic features. [less ▲]

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See detailCharacterization of paragangliomas : a single center cohort study
VROONEN, Laurent ULg; Maiga, Ibrahima ULg; PETROSSIANS, Patrick ULg et al

in Abstract book - 24th Meeting of the Belgian Endocrine Society (2014, October 18)

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See detailCharacterization of Particle Damage and Surface Exposure of a Copper Ore Processed by Jaw Crusher, HPGR and Electro-dynamic Fragmentation
Da Fonseca Martins Gomes, Otavio ULg; Monteiro de Oliveira, Debora; Santos Sobral, Luis Gonzaga et al

in Carpenter, John; Bai, Chenguang; Hwang, Jiann-Yang (Eds.) Characterization of Minerals, Metals, and Materials 2014 (2014)

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See detailCharacterization of particulate debris in retrieval studies on osseointegrated cementless hydroxyapatite-coated polyethylene cups
Masson, Jean-Bernard; Compère, Philippe ULg; Rodriguez, Alfredo et al

in Transactions of the Annual Meeting of the Orthopaedic Research Society (1997), 22

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See detailCharacterization of pectins extracted from banana peels (Musa AAA) under different conditions using an experimental design
Happi Emaga, Thomas ULg; Ronkart, Sébastien ULg; Robert, Christelle et al

in Food Chemistry (2008), 108(2), 463-471

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See detailCharacterization of pentraxin 3 in the horse and its expression in airways.
Ramery, Eve ULg; Fievez, Laurence ULg; Fraipont, Audrey ULg et al

in Veterinary Research (2010), 41(2), 18

The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 ... [more ▼]

The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, western blotting and immuno-histochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: spliced (32 kDa) and full length (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives. [less ▲]

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See detailCharacterization of periodic upper limb movement disorder in a patient with restless arms syndrome.
Ruppert, Elisabeth; Cretin, Benjamin; Meyer, Christelle ULg et al

in Movement Disorders : Official Journal of the Movement Disorder Society (2012), 27(11), 1459-61

BACKGROUND: Restless legs syndrome (RLS) and the frequently associated periodic limb movements (PLM) are common neurological disorders whose pathophysiology remains elusive. We report on the case of a 40 ... [more ▼]

BACKGROUND: Restless legs syndrome (RLS) and the frequently associated periodic limb movements (PLM) are common neurological disorders whose pathophysiology remains elusive. We report on the case of a 40-year-old patient presenting with severe restlessness in the upper limbs, a poorly known variant of RLS. CASE REPORT: Video-polysomnography was performed because of the associated poor sleep quality and daytime sleepiness evocative of PLM. An electromyogram of the extensor carpi radialis muscle was added. Remarkably, our patient had movements of repeated extension of the small finger that contrasted with the extension of the hallux, characteristic for PLM. Pramipexol was an effective treatment relieving the patient's upper limbs of discomfort and ameliorating her restless sleep. CONCLUSIONS: Involvement of the upper limbs in RLS is relatively common, but restlessness may be located on the upper limbs solely. One should be aware of the upper limb variant, and that treatment by dopaminergic agonists proves to be very efficient. [less ▲]

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