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See detailCHARACTERIZATION OF PROTEINURIA IN DOGUE DE BORDEAUX DOGS USING ELECTROPHORESIS AND URINARY BIOMARKERS
Lavoué, Rachel; Smets, P.; Trumel, Cathy et al

in Proceedings of the 2014 ACVIM Forum (2014, June)

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailCharacterization of recombinant wild type and site-directed mutations of apolipoprotein C-III: lipid binding, displacement of ApoE, and inhibition of lipoprotein lipase.
Liu, H.; Talmud, P. J.; Lins, Laurence ULg et al

in Biochemistry (2000), 39(31), 9201-12

The physicochemical properties of recombinant wild type and three site-directed mutants of apolipoprotein C-III (apoC-III), designed by molecular modeling to alter specific amino acid residues implicated ... [more ▼]

The physicochemical properties of recombinant wild type and three site-directed mutants of apolipoprotein C-III (apoC-III), designed by molecular modeling to alter specific amino acid residues implicated in lipid binding (L9T/T20L, F64A/W65A) or LPL inhibition (K21A), were compared. Relative lipid binding efficiencies to dimyristoylphosphatidylcholine (DMPC) were L9T/T20L > WT >K21A > F64A/W65A with an inverse correlation with size of the discoidal complexes formed. Physicochemical analysis (Trp fluorescence, circular dichroism, and GdnHCl denaturation) suggests that L9T/T20L forms tighter and more stable lipid complexes with phospholipids, while F64A/W65A associates less tightly. Lipid displacement properties were tested by gel-filtrating apoE:dipalmitoylphosphatidylcholine (DPPC) discoidal complexes mixed with the various apoC-III variants. All apoC-III proteins bound to the apoE:DPPC complexes; the amount of apoE displaced from the complex was dependent on the apoC-III lipid binding affinity. All apoC-III proteins inhibited LPL in the presence or absence of apoC-II, with F64A/W65A displaying the most inhibition, suggesting that apoC-III inhibition of LPL is independent of lipid binding and therefore of apoC-II displacement. Taken together. these data suggest that the hydrophobic residues F64 and W65 are crucial for the lipid binding properties of apoC-III and that redistribution of the N-terminal helix of apoC-III (L9T/T20L) enhances the stability of the lipid-bound protein, while LPL inhibition by apoC-III is likely to be due to protein:protein interactions. [less ▲]

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See detailCharacterization of regulatory elements in the medaka osterix promoter required for osteoblast expression.
Renn, Jörg ULg; Buettner, A; Chua, EPS et al

in Journal of Applied Ichthyology (2014), 30(4), 652660

The zinc finger transcription factor Osterix/Sp7 is an essential regulator of osteoblastogenesis. In mammals, osterix expression is regulated by Runx2, Msx2 and Dlx5 but recent findings suggest that also ... [more ▼]

The zinc finger transcription factor Osterix/Sp7 is an essential regulator of osteoblastogenesis. In mammals, osterix expression is regulated by Runx2, Msx2 and Dlx5 but recent findings suggest that also retinoic acid plays an important role for osteoblast differentiation and function. Yet, how these and other factors act on the osterix promoter is largely unknown. Expression, knock-down and promoter analyses have indicated that the function of Osterix in osteoblasts is conserved in teleosts and mammals. In the present study, we have used the teleost medaka to identify and characterize a region containing potential retinoic acid response elements in the osterix promoter. We analysed whether this region is important for activity in osteoblasts in vivo, using transgenic medaka lines with modified osterix promoter regions. Promoter activity in vivo and in vitro revealed a short nucleotide sequence in the promoter with crucial positive regulatory function. Mutations of this element lead to a complete inactivation of the osterix promoter in osteoblasts and made it insensitive to retinoic acid treatment. The comparison with the regulatory regions of osterix in other species suggests that the function of this element is highly conserved in vertebrates. [less ▲]

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See detailCharacterization of regulatory functions of the varicella-zoster virus gene-63-encoded protein
Jackers, Pascale ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Journal of Virology (1992), 66(6), 3899-3903

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex ... [more ▼]

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex virus type 1. ICP22 is a polypeptide synthesized in herpes simplex virus type 1-infected cells, and as is the case for its VZV counterpart, its regulatory functions are unknown. On the basis of the VZV DNA sequence, it has been shown that IE63 exhibits hydrophilic and acidic properties, suggesting that this protein could play a regulatory role during the infectious cycle. We report in this article cotransfection experiments which demonstrate that the VZV gene 63 protein strongly represses, in a dose-dependent manner, the expression of VZV gene 62. On the other hand, transient expression of the VZV gene 63 protein can promote activation of the thymidine kinase gene but cannot affect the expression of the genes encoding glycoproteins I and II. The results of transient expression experiments strongly suggest that the VZV gene 63 protein could play a pivotal role in the repression of IE gene expression as well as in the activation of early gene expression [less ▲]

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See detailCharacterization of riparian zones in wallonia (belgium)
Michez, Adrien ULg; Piégay, Hervé; Lejeune, Philippe et al

Conference (2014, June 16)

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to ... [more ▼]

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to regional scale (ca. 13000 km for 1000 management sectors). The aim of this remotely sensed monitoring is to improve the river and riparian zone management and planning by providing some key information for river managers. This first research is exclusively based on data which are available at the regional scale (170000 square km) to develop automated tools to implement the methodology to the whole Walloon river network (13000 km) before 2015 with an update frequency of 3 years (photogrammetric DSM survey frequency). [less ▲]

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See detailCharacterization of riparian zones in wallonia (belgium) from local to regional scale using aerial lidar data and photogrammetric DSM
Michez, Adrien ULg; Piégay, Hervé; Lejeune, Philippe et al

in EARSeL eProceedings (2014), 13(2),

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to ... [more ▼]

The present study proposes an innovative approach to automatically extract riparian zone characteristics in order to assess its quality, from pre-determined river management reaches (1-3 km long) to regional scale (ca. 13000 km for 1000 management sectors). The aim of this remotely sensed monitoring is to improve the river and riparian zone management and planning by providing some key information for river managers. The methodology was developed based on two watersheds covering approximately 500 km of river network (ca. 200 management sectors). The riparian zone quality is evaluated through various indicators of its ecological integrity (e.g. longitudinal continuity of riparian forest, mean vegetation height and relative standard deviation), hydromorphological quality and physical settings (e.g. flow channel extent, floodplain width, channel sinuosity). The physical characteristics of the riparian zone are mainly extracted from a high quality Digital Terrain Model (derived from ALS data) while the attributes of the riparian forest are derived from a « hybrid » Canopy Height Model (photogrammetric Digital Surface Model – LiDAR derived DTM). This first research is exclusively based on data which are available at the regional scale (170000 square km) to develop automated tools to implement the methodology to the whole Walloon river network (13000 km) before 2015 with an update frequency of 3 years (photogrammetric DSM survey frequency). Moreover, our approach is based on a photogrammetric Digital Surface Model which is derived from raw images of an orthophoto coverage. As most of European countries are regularly covered by orthophoto surveys, our approach is widely replicable in countries where a quality DTM is available. [less ▲]

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See detailCharacterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD
Odjo, Djosse Psijus Sylvanus ULg; Bera, François ULg; Jacquet, Nicolas ULg et al

in Stärke = Starch (2016), 68

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric ... [more ▼]

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs. [less ▲]

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See detailCharacterization of seawater intrusions using 2D electrical tomography
Nguyen, Frédéric ULg; Kemna, A.; Antonsson, A. et al

in Near Surface Geophysics (2009), 7

We have investigated the potential of 2D electrical imaging for the characterization of seawater intrusion using field data from a site in Almeria, SE Spain. Numerical simulations have been run for ... [more ▼]

We have investigated the potential of 2D electrical imaging for the characterization of seawater intrusion using field data from a site in Almeria, SE Spain. Numerical simulations have been run for several scenarios, with a hydrogeological model reflecting the local site conditions. The simulations showed that only the lower salt concentrations of the seawater-freshwater transition zone could be recovered, due to the loss of resolution with depth. We quantified this capability in terms of the cumulative sensitivity associated with the measurement setup and showed that the mismatch between the targeted and imaged parameter values occurs from a certain sensitivity threshold. Similarly, heterogeneity may only be determined accurately if located in an adequately sensitive area. At the field site, we identified seawater intrusion at the scale of a few kilometres down to a hundred metres. Borehole logs show a remarkable correlation with the image obtained from surface data but indicate that the electrically derived mass fraction of pure seawater could not be recovered due to the discrepancy between the in-situ and laboratory-derived petrophysical relationships. Surface-to-hole inversion results suggest that the laterally varying resolution pattern associated with such a setup dominates the image characteristics compared to the laterally more homogeneous resolution pattern of surface only inversion results and hence, surface-to-hole images are not easily interpretable in terms of larger-scale features. Our results indicate that electrical imaging can be used to constrain seawater intrusion models if image appraisal tools are appropriately used to quantify the spatial variation of sensitivity and resolution. The most crucial limitation is probably the apparent non-stationarity of the petrophysical relationship during the imaging process [less ▲]

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See detailCharacterization of serracin P, a phage-tail-like bacteriocin, and its activity against Erwinia amylovora, the fire blight pathogen
Jabrane, A.; Sabri, Ahmed ULg; Compère, Philippe ULg et al

in Applied and Environmental Microbiology (2002), 68(11), 5704-5710

Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora,the causal agent of the most serious bacterial disease of apple and pear trees, fire ... [more ▼]

Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora,the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon induction with mitomycin C. A phage-tail-like bacteriocin, named serracin P, was purified from an induced culture supernatant of S. plymithicum J7. It was found to be the only compound involved in the antibacterial activity against sensitive strains. The N-terminal amino acid sequence analysis of the two major subunits (23 and 43 kDa) of serracin P revealed high homology with the Fels-2 prophage of Salmonella enterica, the coliphages P2 and 168, the CTX prophage of Pseudomonas aeruginosa, and a prophage of Yersinia pestis. This strongly suggests a common ancestry for serracin P and these bacteriophages. [less ▲]

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See detailCharacterization of somatostatin transactivating factor-1, a novel homeobox factor that stimulates somatostatin expression in pancreatic islet cells.
Peers, Bernard ULg; Johnson, T.; Ferreri, K. et al

in Molecular Endocrinology (1993), 7(10), 1275-83

The endocrine pancreas consists of several differentiated cell types that are distinguished by their selective expression of peptide hormones such as insulin, glucagon, and somatostatin. Although a number ... [more ▼]

The endocrine pancreas consists of several differentiated cell types that are distinguished by their selective expression of peptide hormones such as insulin, glucagon, and somatostatin. Although a number of homeobox-type factors have been proposed as key regulators of individual peptide genes in the pancreas, their cellular distribution and relative abundance remain uncharacterized. Also, their overlapping DNA binding specificities have further obscured the regulatory functions these factors perform during development. In this report we characterize a novel homeobox-type somatostatin transactivating factor termed STF-1, which is uniformly expressed in cells of the endocrine pancreas and small intestine. The 283-amino acid STF-1 protein binds to tissue-specific elements within the somatostatin promoter and stimulates somatostatin gene expression both in vivo and in vitro. Remarkably, STF-1 comprises the predominant tissue-specific element-binding activity in nuclear extracts from somatostatin-producing pancreatic islet cells, suggesting that this protein may have a primary role in regulating peptide hormone expression and specifying endocrine cell lineage in the developing gut. [less ▲]

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See detailCharacterization of some functions stable for positive linear transformations
Marichal, Jean-Luc; Mathonet, Pierre ULg; Tousset, E.

in Fuzzy Sets & Systems (1999), 102

This paper deals with a characterization of a class of aggregation operators. This class concerns operators which are symmetric, increasing, stable for the same positive linear transformations and present ... [more ▼]

This paper deals with a characterization of a class of aggregation operators. This class concerns operators which are symmetric, increasing, stable for the same positive linear transformations and present a property close to the bisymmetry property: the ordered bisymmetry property. It is proved that the class investigated contains exactly the ordered weighted averaging operators (OWA) introduced by Yager in 1988. [less ▲]

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See detailCharacterization of spatio-temporal organization of slow waves during human NREM sleep
Schrouff, Jessica ULg; Leclercq, Yves ULg; Foret, Ariane et al

Poster (2009, December 14)

Sleep is a behavior commonly observed in a large number of animal species. However, neuroscientists still poorly understand the meaning of this loss of consciousness absolutely needed for life. In the ... [more ▼]

Sleep is a behavior commonly observed in a large number of animal species. However, neuroscientists still poorly understand the meaning of this loss of consciousness absolutely needed for life. In the present work, we established different methods to characterize the Slow Wave Sleep most recognizable patterns: the Slow Waves (SWs). Since the anatomical structure of white matter tracts that connect various brain regions is not random and thus must constraint the propagation of waves (Hagmann et al., 2008), our basic hypothesis was that large white matter bundles would bias the propagation of SW along specific patterns, which could be identified in homogeneous clusters of waves. To investigate our hypothesis, SWs were detected automatically on the three first periods of SWS using an algorithm based on Massimini et al., 2004. They were then clustered using a two steps procedure involving a hierarchical clustering based on delay maps and a k-means clustering based on the SWs potential in a given time interval around the maximum power of the SW negative peak. To compute the relevance of the final clusters, a mathematical criterion was implemented as well as a visual check. Results of the multisubjects study showed that only bad quality and small clusters could be obtained, suggesting that there is no particular organization of SWs across the night and inforcing the hypothesis that SWs are local phenomena, each one decreasing the homeostatic pressure in only one specific area. [less ▲]

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See detailCharacterization of spontaneous bone marrow recovery after sublethal total body irradiation: importance of the osteoblastic/adipocytic balance.
Poncin, Géraldine ULg; Beaulieu, Aurore ULg; Humblet, Chantal ULg et al

in PLoS ONE (2012), 7(2), 30818

Many studies have already examined the hematopoietic recovery after irradiation but paid with very little attention to the bone marrow microenvironment. Nonetheless previous studies in a murine model of ... [more ▼]

Many studies have already examined the hematopoietic recovery after irradiation but paid with very little attention to the bone marrow microenvironment. Nonetheless previous studies in a murine model of reversible radio-induced bone marrow aplasia have shown a significant increase in alkaline phosphatase activity (ALP) prior to hematopoietic regeneration. This increase in ALP activity was not due to cell proliferation but could be attributed to modifications of the properties of mesenchymal stem cells (MSC). We thus undertook a study to assess the kinetics of the evolution of MSC correlated to their hematopoietic supportive capacities in mice treated with sub lethal total body irradiation. In our study, colony-forming units - fibroblasts (CFU-Fs) assay showed a significant MSC rate increase in irradiated bone marrows. CFU-Fs colonies still possessed differentiation capacities of MSC but colonies from mice sacrificed 3 days after irradiation displayed high rates of ALP activity and a transient increase in osteoblastic markers expression while ppargamma and neuropilin-1 decreased. Hematopoietic supportive capacities of CFU-Fs were also modified: as compared to controls, irradiated CFU-Fs significantly increased the proliferation rate of hematopoietic precursors and accelerated the differentiation toward the granulocytic lineage. Our data provide the first evidence of the key role exerted by the balance between osteoblasts and adipocytes in spontaneous bone marrow regeneration. First, (pre)osteoblast differentiation from MSC stimulated hematopoietic precursor's proliferation and granulopoietic regeneration. Then, in a second time (pre)osteoblasts progressively disappeared in favour of adipocytic cells which down regulated the proliferation and granulocytic differentiation and then contributed to a return to pre-irradiation conditions. [less ▲]

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See detailCharacterization of spontaneous collagen fibrillogenesis in a cell-free and tension-free environment.
PIERARD, Gérald ULg; Hermanns-Lê, Trinh ULg; Delvenne, Philippe ULg et al

in Clinical & Experimental Dermatology (2013)

The collagen fibril packing that forms threads and bundles is poorly defined, despite the fact that it is important for distinct aspects of the adventitial and reticular dermis. The present study explored ... [more ▼]

The collagen fibril packing that forms threads and bundles is poorly defined, despite the fact that it is important for distinct aspects of the adventitial and reticular dermis. The present study explored an in vitro fibrillogenesis model using the property of heat polymerization. The process was performed on glass slides with mixtures of collagen I and III, and the material was viewed by scanning electron microscopy. In all instances, collagen I and III formed fibrils with regular sizes. The formation of threads was influenced by the relative proportions of collagen I and III; increasing the relative proportion of collagen I resulted in the formation of threads showing increasing variations in thickness. These findings are in line with the differential presentation and compositions of the different parts of the dermis. The possible interventions of stromal cells and of other macromoleules of the extracellular matrix were not considered in this study. [less ▲]

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See detailCHARACTERIZATION OF STONY SOILS’ HYDRAULIC PROPERTIES AND REPRESENTATIVE ELEMENTARY VOLUME USING FIELD, LABORATORY AND NUMERICAL EXPERIMENTS
Pichault, Mathieu ULg

Master's dissertation (2015)

Determining soil hydraulic properties is of major concern in various fields of study such as soil physics, hydrology, ecology and agronomy. Though stony soils are widespread across the globe, most studies ... [more ▼]

Determining soil hydraulic properties is of major concern in various fields of study such as soil physics, hydrology, ecology and agronomy. Though stony soils are widespread across the globe, most studies deal with gravel-free soils so that the literature describing the impact of stones on a soil’s hydraulic characteristics is still rather scarce. The aim of this study is to assess, through a case study of a clayey soil in Thailand, the effect of rock fragments on the main hydraulic characteristics, i.e. hydraulic conductivity and retention curves, and on related physical properties of the soil. This was done by means of field, laboratory and numerical experiments involving different amounts and types of coarse fragments. Results were compared with those predicted by several models assuming that stones are non-porous and only reduce the cross-sectional area available for water flow. These models further consider that the shape parameters of the retention and hydraulic conductivity curves are not dependent on the stoniness. We tested the validity of such assumptions. We did not find evidence against the assumption stating that stones might be considered as non-porous. However, our results suggest that the shape parameters of the retention curve vary according to the stone content. Furthermore, considering that stones only reduce the cross-sectional area available for water flow might also be ill-founded. We pointed out several significant drivers of the saturated hydraulic conductivity which are not considered by these models. First of all, the effect due to the creation of voids at the fine earth-stone interface combined with an increased difficulty to repack the fine earth can counteract and even overcome the effect of a reduced cross-sectional area in some cases. Moreover, the shape and the size of inclusions may also affect substantially hydraulic conductivity. Finally, we developed a code destined to quantify the representative elementary volume of soils containing different amounts of stones of various diameters. [less ▲]

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See detailCharacterization of stream - aquifer interaction in carbonate rocks
Briers, Pierre ULg; Sohier, Catherine ULg; Schmit, Flore et al

Poster (2014, September 30)

Groundwater - surface water interactions play a fundamental role in terms of quantity and quality of water and in terms of ecological quality of rivers. Despite many research efforts and the necessity to ... [more ▼]

Groundwater - surface water interactions play a fundamental role in terms of quantity and quality of water and in terms of ecological quality of rivers. Despite many research efforts and the necessity to better understand such interactions in order to reach effective management of water resources, stream-aquifer exchanges remain poorly understood, in particular in fractured carbonate environments. [less ▲]

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See detailCharacterization of structures with a complex disposition of YBCO coated conductors for magnetic shielding applications
Wera, Laurent ULg; Fagnard, Jean-François ULg; Levin, G. A. et al

Poster (2013, September 16)

An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free ... [more ▼]

An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free superconducting loop where persistent currents can flow and provide a strong attenuation of a magnetic field. Our previous works have shown that this assembly is able to shield an axial quasi static (“DC”) magnetic field and that the shielding performances depend on the aspect ratio and the number of layers. The purpose of the present work is to study experimentally the shielding efficiency of several structures with a more complex orientation and position of YBCO coated conductors. Our aim is to design a magnetic shield that would be able to shield a magnetic field directed at any angle with respect to the superconducting loops. Such a structure can be obtained by placing pairs of coated conductors sections along three orthogonal axes. All experiments are carried out at 77K. The structure is subjected to a quasi-static (“DC”) magnetic field. A Hall probe measures the three components of the local magnetic induction inside the assembly as a function of the applied magnetic induction. The shielding efficiency of the structure is characterized as a function of (i) the magnetic field amplitude, (ii) the position of the Hall probe along the three axes, and (iii) the angles between the applied magnetic field and each axis. The experimental results allow us to determine the shielding efficiency in the central part of the new 3-axes structure. Although the shielding efficiency is lowered with respect to that of the traditional 1-axis-coil geometry, measurements at different field orientations allow us to identify the role played by each of the pairs of coils in screening the external magnetic field. [less ▲]

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