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See detailChlamydomonas reinhardtii proteomics adaptations in response to the absence of the energy-dissipating alternative oxidase
Mathy, Grégory ULg

Poster (2008, May 28)

The Alternative oxidase (AOX) is an ubiquinol-oxygen oxidoreductase found in the mitochondrial inner membrane of plants, fungi and protists. In mitochondria, AOX activation creates an electron ... [more ▼]

The Alternative oxidase (AOX) is an ubiquinol-oxygen oxidoreductase found in the mitochondrial inner membrane of plants, fungi and protists. In mitochondria, AOX activation creates an electron partitioning between the cytochrome pathway (CIII + CIV) and AOX. This partitioning leads to a decrease of proton pumping efficiency by the respiratory chain complexes per O2 consumed. Two closely related physiological roles are attributed to AOX: First, AOX in conjunction with rotenone insensitive NADH dehydrogenases, generates a fully non-coupled (energy dissipative) electron transport chain in the mitochondria, which is believed to play an important role in regenerating oxidized cofactors required for others metabolic demands. The second proposed role of AOX is to prevent an important increase the QR/Qt ratio and consequently, to prevent reactive oxygen species (ROS) production. In Chlamydomonas reinhartii AOX is encoded by two different genes, the AOX1 gene being much more transcribed than AOX2. In addition, the expression of the AOX1 gene is generally unresponsive to a number of known AOX allosteric effectors, but is down-regulated by ammonium and up-regulated by nitrate. In the present work, we performed a comparative proteomic study of isolated mitochondria by using the 2D-DIGE methodology to evidence the effects of AOX1 silencing on Chlamydomonas mitochondrial soluble proteome cultivated on nitrate in myxotrophic conditions [less ▲]

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See detailChlamydophila felis infection. ABCD guidelines on prevention and management
Gruffydd-Jones, T.; Addie, D.; Belak, B. et al

in Journal of Feline Medicine and Surgery (2009), 11

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See detailChlamydophila psittaci chez les canards d’élevages et d’ornement en Région Wallonne
Delleur, Valery ULg; Marlier, Didier ULg

in Proceedings du 3ème colloque international de bactériologie francophone (2006, July)

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See detailChlordecone Exposure and Risk of Prostate Cancer
Multigner, Luc; NDong, Jean-Rodrigue; Giusti, Arnaud ULg et al

in Journal of Clinical Oncology (2010)

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See detailChloride and Inorganic Phosphate Modulate Binding of Oxygen to Bovine Red Blood Cells
Gustin, Pascal ULg; Detry, Benoît; Cao, M. L. et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1994), 77(1), 202-208

The influence of Pi and Cl on the equilibrium of oxygen binding to bovine red blood cells was assessed by plotting the whole blood oxygen dissociation curve measured under standard conditions with and ... [more ▼]

The influence of Pi and Cl on the equilibrium of oxygen binding to bovine red blood cells was assessed by plotting the whole blood oxygen dissociation curve measured under standard conditions with and without added KCl and K2HPO4. Both salts shifted the oxygen dissociation curve to the right. This effect was more marked at the highest saturation levels. At a given saturation level, the anion-induced shift was linearly related to the concentration of salt added to the blood. Cl had a greater effect than Pi. The relationship between changes in Po2 at 50% hemoglobin saturation (in Torr) and concentrations of ions added (in mmol/l) was equal to 0.0515[Cl] + 0.0302[Pi] (r2 = 0.94; P < 0.001). These changes were not due to the hyperosmolality induced by salt addition, since sucrose added in place of salts was without effect on the measured parameters. The oxygen exchange fraction expressed as percentage of saturation, i.e., the difference in hemoglobin saturation when Po2 decreases from 130 to 40 Torr, was linearly correlated to added anion concentration (in mmol/l) (= 0.102[Cl] + 0.059[Pi] (r2 = 0.95; P < 0.001)). No significant interaction between the anions was observed; their effects were purely additive. This original mechanism of controlling the oxygen affinity of bovine blood may have clinical relevance: Cl and Pi could be used to increase oxygen transport efficiency in hypoxic animals [less ▲]

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See detailChloride channels and endocytosis: new insights from Dent's disease and ClC-5 knockout mice.
Devuyst, Olivier; JOURET, François ULg; Auzanneau, Celine et al

in Nephron. Clinical Practice (2005), 99(3), 69-73

Dent's disease is a hereditary renal tubular disorder characterized by low-molecular weight (LMW) proteinuria, hypercalciuria and nephrolithiasis. The disease is due to mutations of ClC-5, a member of the ... [more ▼]

Dent's disease is a hereditary renal tubular disorder characterized by low-molecular weight (LMW) proteinuria, hypercalciuria and nephrolithiasis. The disease is due to mutations of ClC-5, a member of the family of voltage-gated CLC chloride channels. ClC-5 is expressed in part in cells lining the proximal tubule (PT) of the kidney, where it colocalizes with albumin-containing endocytic vesicles belonging to the receptor-mediated endocytic pathway that ensures efficient reabsorption of ultrafiltrated LMW proteins. Since progression along the endocytic apparatus requires endosomal acidification, it has been suggested that dysfunction of ClC-5 in endosomes may lead to inefficient reabsorption of LMW proteins and dysfunction of PT cells. Analysis of a ClC-5 knockout (KO) mouse model, displaying all the characteristic renal tubular defects of Dent's disease, showed evidence of a severe LMW proteinuria. Cytochemical studies with the endocytic tracer, peroxidase, showed poor transfer into early endocytic vesicles, suggesting that impairment of receptor-mediated endocytosis in PT cells is the basis for the defective uptake of LMW proteins in patients with Dent's disease. Endocytosis and processing of LMW proteins involve the multiligand tandem receptors, megalin and cubilin, that are abundantly expressed at the brush border of PT cells. Characterization of the endocytic defect in ClC-5 KO mice revealed that ligands of both megalin and cubilin were affected. The total kidney content of megalin and especially cubilin at the protein level was decreased but, more importantly, using analytical subcellular fractionation and quantitative immunogold labelling we demonstrated a selective disappearance of megalin and cubilin at the brush border of PT cells. These observations allowed us to conclude that defective protein endocytosis linked to ClC-5 inactivation is due at least in part to a major and selective loss of megalin and cubilin at the brush border, reflecting a trafficking defect in renal PT cells. These results improve our understanding of Dent's disease, taken as a paradigm for renal Fanconi syndrome and nephrolithiasis, and demonstrate multiple roles for ClC-5 in the kidney. These studies also provided insights into important functions such as apical endocytosis, handling of proteins by renal tubular cells, calcium metabolism, and urinary acidification. [less ▲]

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See detailChloride Permeability of Rat Brain Membrane Vesicles Correlates with Thiamine Triphosphate Content
Bettendorff, Lucien ULg; Hennuy, Benoît ULg; De Clerck, Anne et al

in Brain Research (1994), 652(1), 157-160

Incubation of rat brain homogenates with thiamine or thiamine diphosphate (TDP) leads to a synthesis of thiamine triphosphate (TTP). In membrane vesicles subsequently prepared from the homogenates ... [more ▼]

Incubation of rat brain homogenates with thiamine or thiamine diphosphate (TDP) leads to a synthesis of thiamine triphosphate (TTP). In membrane vesicles subsequently prepared from the homogenates, increased TTP content correlates with increased 36Cl- uptake. A hyperbolic relationship was obtained with a K0.5 of 0.27 nmol TTP/mg protein. In crude mitochondrial fractions from the brains of animals previously treated with thiamine or sulbutiamine, a positive correlation between 36Cl- uptake and TTP content was found. These results, together with other results previously obtained with the patch-clamp technique, suggest that TTP is an activator of chloride channels having a large unit conductance. [less ▲]

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See detail(Chloro-4 phenyl) thiomethylene bisphosphonate in Paget’s bone disease
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Lecart, MP et al

in Acta Belgica. Medica Physica (1989), 12

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See detail(Chloro-4-phenyl) thiomethylene bisphosphonate in Paget's bone disease
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Lecart, MP et al

in Journal Belge de Médecine Physique et de Réhabilitation = Belgisch Tijdschrift voor Fysische Geneeskunde en Rehabilitatie (1989), 12

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See detailChlorophyll Synthesis in Dark-Grown Pine Primary Needles
Schoefs, B.; Franck, Fabrice ULg

in Plant Physiology (1998), 118(4), 1159-68

The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of ... [more ▼]

The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously demonstrated that pine primary needles are able to synthesize chlorophyll in the dark. Norflurazon strongly inhibited both carotenoid and chlorophyll synthesis. Needles of plants treated with this inhibitor had low chlorophyll content, contained only traces of xanthophylls, and accumulated carotenoid precursors. The first form of chlorophyll detected in young pine needles grown in darkness had an emission maximum at 678 nm. Chlorophyll-protein complexes with in situ spectroscopic properties similar to those of fully green needles (685, 695, and 735 nm) later accumulated in untreated plants, whereas in norflurazon-treated plants the photosystem I emission at 735 nm was completely lacking. To better characterize the light-dependent chlorophyll biosynthetic pathway in pine needles, the 77 K fluorescence properties of in situ protochlorophyllide a spectral forms were studied. Photoactive and nonphotoactive protochlorophyllide a forms with emission properties similar to those reported for dark-grown angiosperms were found, but excitation spectra were substantially red shifted. Because of their lower chlorophyll content, norflurazon-treated plants were used to study the protochlorophyllide a photoreduction process triggered by one light flash. The first stable chlorophyllide photoproduct was a chlorophyllide a form emitting at 688 nm as in angiosperms. Further chlorophyllide a shifts usually observed in angiosperms were not detected. The rapid regeneration of photoactive protochlorophyllide a from nonphotoactive protochlorophyllide after one flash was demonstrated. [less ▲]

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See detailChlorophyll synthesis in relation to the assembly of photosystems
Franck, Fabrice ULg; Schoefs, Benoît

in Bulletin de la Société Royale des Sciences de Liège (1996), 65(4-5), 269-278

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See detailChlorophyll: natural sources, extraction methods and application for textile industry
Miazek, Krystian ULg; Remacle, Claire ULg; Richel, Aurore ULg et al

Conference (2014, October 14)

Chlorophyll is a photosynthetic pigment available abundantly in microalgae and terrestrial plants. This pigment found applications in pharmaceutical, cosmetic and food products as a wound healing ... [more ▼]

Chlorophyll is a photosynthetic pigment available abundantly in microalgae and terrestrial plants. This pigment found applications in pharmaceutical, cosmetic and food products as a wound healing, antioxidant or coloring agent. Recent reports suggest that chlorophyll can also be used as a biomordant to enhance the dyeing process of textile products, but also as a textile dye with antimicrobial properties. In this presentation, different aspects of chlorophyll production are discussed. Firstly, numerous plant biomass types as potential sources of chlorophyll are presented. Subsequently, different methods for chlorophyll extraction and separation from plant biomass are described. Finally, possibilities of chlorophyll implementation into textile products on industrial scale are evaluated. [less ▲]

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See detailChloroplastic and mitochondrial metal homeostasis.
Nouet, Cécile ULg; Motte, Patrick ULg; Hanikenne, Marc ULg

in Trends in Plant Science (2011), 16(7), 395-404

Transition metal deficiency has a strong impact on the growth and survival of an organism. Indeed, transition metals, such as iron, copper, manganese and zinc, constitute essential cofactors for many key ... [more ▼]

Transition metal deficiency has a strong impact on the growth and survival of an organism. Indeed, transition metals, such as iron, copper, manganese and zinc, constitute essential cofactors for many key cellular functions. Both photosynthesis and respiration rely on metal cofactor-mediated electron transport chains. Chloroplasts and mitochondria are, therefore, organelles with high metal ion demand and represent essential components of the metal homeostasis network in photosynthetic cells. In this review, we describe the metal requirements of chloroplasts and mitochondria, the acclimation of their functions to metal deficiency and recent advances in our understanding of their contributions to cellular metal homeostasis, the control of the cellular redox status and the synthesis of metal cofactors. [less ▲]

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See detailChoc anaphylactique peri-anesthesique: mythe et realites
Van Den Bosch, Sabine ULg; Libbrecht, Dominique ULg; Lamy, Maurice ULg

in Revue Médicale de Liège (2004), 59(5), 336-44

Peri-anaesthetic anaphylactic shock is a fortunately rare event, which nevertheless often turns out to be life-threatening. Only fast recognition and reaction can permit to avoid its sometimes dramatic ... [more ▼]

Peri-anaesthetic anaphylactic shock is a fortunately rare event, which nevertheless often turns out to be life-threatening. Only fast recognition and reaction can permit to avoid its sometimes dramatic consequences. This implies, from the anaesthetist, good knowledge of the mechanisms, of the events'sequence, of the usual triggering drugs and, of course, of the anaphylactic shock's treatment. After such an accident has occured the patient should be referred to an allergy specialist in order to perform a thorough exploration, thus pointing out which drug is responsible for the reaction, and by which mechanism. Drugs to be banished in the future would so be identified and should be taken to the patient's as well as his general practician's knowledge. [less ▲]

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