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See detailCD44v6 expression is an independent prognostic factor in node-negative FIGO stage IB cervical carcinoma.
Speiser, P.; KRIDELKA, Frédéric ULg; Tempfer, C. et al

in International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society (1999), 9(2), 160-165

Adhesion molecules such as CD44 play an important role in the metastatic cascade by mediating tumor cell interaction with the endothelium and the subendothelial matrix. As a so-called "lymphocyte homing ... [more ▼]

Adhesion molecules such as CD44 play an important role in the metastatic cascade by mediating tumor cell interaction with the endothelium and the subendothelial matrix. As a so-called "lymphocyte homing receptor," CD44 is physiologically involved in migration of circulating lymphocytes to lymphatic tissue. In the present study, we investigated the expression of CD44v3 and v6 in 237 patients with stage IB, N0 cervical carcinoma by means of immunohistochemistry. These results were correlated with the GOG score and other prognostic variables. Median follow-up was 82.6 months (39-110 months). Thirty-nine patients recurred and 35 died from disease within the observation period. In univariate analysis, the GOG score, histologic subtype, and CD44v6 expression were statistically significant predictors for poor overall survival (OS). In multivariate (Cox regression) analysis, the GOG score (< 40 vs. 40-120, RR: 1.37 (95% CI: 1.10-1.71); 40-120 vs. > 120, RR: 2.23 (95% CI: 1.28-3.88); P = 0.004), histologic subtype (adenosquamous carcinomas) (RR: 4.56 (95% CI: 1.49-13.92), P = 0.007) and CD44v6 expression (RR: 2.42 (95% CI: 1.14-5.10), P = 0.021) were independent predictors for poor OS. The expression of CD44v3 did not correlate with prognosis. Furthermore we found a strong correlation between CD44v6 expression and lymphovascular space invasion (LVSI) (chi2 = 17.01, P = 0.0001). Tumor expansion into the loco-regional lymphatic system is the preferred way of tumor spread in cervical carcinoma. The strong correlation of CD44v6 with LVSI produces a significant degree of suspicion that cervical carcinoma cells expressing CD44v6 could, by mimicking lymphocytes, exploit their pathways. [less ▲]

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See detailCD68 and Factor XIIIa expressions in granular-cell tumor of the skin
Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg; Pierard-Franchimont, Claudine ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1993), 186(2), 106-108

Immunohistochemical and electron microscopic studies support a Schwann cell origin for most of the granular-cell tumors of the skin. We studied 9 granular-cell tumors by immunohistochemistry, using ... [more ▼]

Immunohistochemical and electron microscopic studies support a Schwann cell origin for most of the granular-cell tumors of the skin. We studied 9 granular-cell tumors by immunohistochemistry, using antibodies to CD68, S-100 proteins, factor XIIIa, L1 antigen, desmin and vimentin. The CD68 monocyte-macrophage-associated lysosomal antigen was expressed in granular cells which however had no Mac 387 reactivity. The 'satellite fibroblasts' were factor XIIIa-positive similarly to dendrocytes and perineurial cells. Our study reveals the lack of specificity of CD68 antibody to identify macrophages. It also presents granular cell tumor of the skin as an exception in the group of schwannomas by the presence of factor XIIIa-positive cells inside the neoplasm. [less ▲]

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See detailCdc42 downregulates MMP-1 expression by inhibiting the ERK1/2 pathway.
Deroanne, Christophe ULg; Hamelryckx, Delphine; Ho, Thi Thanh Giang ULg et al

in Journal of Cell Science (2005), 118(Pt 6), 1173-83

The small GTPases of the Rho family are key intermediates in cellular signalling triggered by activated cell-adhesion receptors. In this study, we took advantage of RNA interference (RNAi) using small ... [more ▼]

The small GTPases of the Rho family are key intermediates in cellular signalling triggered by activated cell-adhesion receptors. In this study, we took advantage of RNA interference (RNAi) using small interfering RNAs (siRNAs) to define the roles of the best-characterized members of the RhoGTPase family, RhoA, Rac1 and Cdc42, in the control of MMP-1, MMP-2 and type-I-collagen expression in normal human skin fibroblasts (HSFs). A specific and long-lasting repression, up to 7 days after transfection, of the three GTPases was achieved by transient transfection of specific siRNA. The silencing of Cdc42, but not that of RhoA or Rac1, induced a 15-fold increase in MMP-1 secretion. This upregulation was confirmed at the mRNA level and observed with two different siRNAs targeting Cdc42. Such a regulation was also observed in various human cell lines and was rescued by re-expressing wild-type Cdc42 encoded by a construct bearing silent mutations impeding its recognition by the siRNA. By contrast, MMP-2 and type-I-collagen expression was not affected by the individual silencing of each Rho GTPase. Cytokine protein array, enzyme-linked immunosorbent assays and reverse-transcription PCR measurements revealed that ablation of Cdc42 induced an overexpression of interleukin 8 and MCP-1. Although these cytokines are known to induce the expression of MMP-1, we showed that they were not involved in the Cdc42-mediated upregulation of MMP-1. Silencing of Cdc42 also induced an increased phosphorylation of ERK1/2 and p38 MAP kinase. The use of chemical inhibitors on Cdc42-ablated cells revealed that the upregulation of MMP-1 is dependent on the ERK1/2 pathways, whereas the p38 MAP kinase pathway displayed an inhibitory role. Simultaneous knock-down of two or three Rho GTPases allowed us to demonstrate that the RhoA-ROCK pathway was not involved in this regulation but that the silencing of Rac1 reduced the effect of Cdc42 suppression. These data suggest that, in vivo, when cell/extracellular-matrix interactions via integrins induce cytoskeleton organization, MMP-1 expression is maintained at a low level by Cdc42 via a repression of the Rac1 and ERK1/2 pathways. Therefore, Cdc42 contributes to ECM homeostasis and connective tissue integrity. [less ▲]

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See detailCdI2 structure type as potential thermoelectric materials: Synthesis and high temperature thermoelectric properties of the solid solution TiSxSe2−x
Franck, Gascoin; Nunna, Raghavendra ULg; Emmanuel, Guilmeau et al

in Journal of Alloys & Compounds (2012), 521

Polycrystalline samples of the solid solution TiSxSe2−x with x varying from 0 to 2 were prepared using direct high temperature reaction of stoichiometric amounts of the elements. Rietveld refinements of ... [more ▼]

Polycrystalline samples of the solid solution TiSxSe2−x with x varying from 0 to 2 were prepared using direct high temperature reaction of stoichiometric amounts of the elements. Rietveld refinements of powder X-ray diffraction data are consistent with the existence of a full solid solution. High temperature Seebeck coefficient, electrical resistivity and thermal diffusivity measurements were performed on pellets densified by spark plasma sintering. These measurements reveal that along the solid solution the transport properties vary from the rather metallic and p-type character of TiSe2 to the semiconducting and n-type of TiS2. This change of conduction regime is responsible for the peculiar evolutions of transport properties of TiS0.5Se1.5 with increasing temperature that vary somewhat differently than that of the other members of the solid solution. As expected, the disorder generated by the mixed occupancy of the S and Se on the anionic site is responsible for the diminution of the lattice thermal conductivity. A maximum zT above 0.4 at 400 °C is reached for TiS1.5Se0.5. [less ▲]

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See detailCdk2 is critical for proliferation and self-renewal of neural progenitor cells in the adult subventricular zone
Jablonska, Beata; Aguirre, Adan A.; Vandenbosch, Renaud ULg et al

in Journal of Cell Biology (2007), 179(6), 1231-1245

We investigated the function of cyclin-dependent kinase 2 (Cdk2) in neural progenitor cells during postnatal development. Chondroitin sulfate proteoglycan (NG2)-expressing progenitor cells of the ... [more ▼]

We investigated the function of cyclin-dependent kinase 2 (Cdk2) in neural progenitor cells during postnatal development. Chondroitin sulfate proteoglycan (NG2)-expressing progenitor cells of the subventricular zone (SVZ) show no significant difference in density and proliferation between Cdk2(-/-) and wild-type mice at perinatal ages and are reduced only in adult Cdk2(-/-) mice. Adult Cdk2(-/-) SVZ cells in culture display decreased self-renewal capacity and enhanced differentiation. Compensatory mechanisms in perinatal Cdk2(-/-) SVZ cells, which persist until postnatal day 15, involve increased Cdk4 expression that results in retinoblastoma protein inactivation. A subsequent decline in Cdk4 activity to wild-type levels in postnatal day 28 Cdk2(-/-) cells coincides with lower NG2(+) proliferation and self-renewal capacity similar to adult levels. Cdk4 silencing in perinatal Cdk2(-/-) SVZ cells abolishes Cdk4 up-regulation and reduces cell proliferation and self-renewal to adult levels. Conversely, Cdk4 overexpression in adult SVZ cells restores proliferative capacity to wildtype levels. Thus, although Cdk2 is functionally redundant in perinatal SVZ, it is important for adult progenitor cell proliferation and self-renewal through age-dependent regulation of Cdk4. [less ▲]

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See detailCdk2 Is Dispensable for Adult Hippocampal Neurogenesis
Vandenbosch, Renaud ULg; Borgs, Laurence ULg; Beukelaers, Pierre ULg et al

in Cell Cycle (Georgetown, Tex.) (2007), 6(24), 3065-9

Granule neurons of the dentate gyrus (DG) of the hippocampus undergo continuous renewal throughout life. Among cell cycle regulators, cyclin-dependent kinase 2 (Cdk2) is considered as a major regulator of ... [more ▼]

Granule neurons of the dentate gyrus (DG) of the hippocampus undergo continuous renewal throughout life. Among cell cycle regulators, cyclin-dependent kinase 2 (Cdk2) is considered as a major regulator of S-phase entry. We used Cdk2-deficient mice to decipher the requirement of Cdk2 for the generation of new neurons in the adult hippocampus. The quantification of cell cycle markers first revealed that the lack of Cdk2 activity does not influence spontaneous or seizure-induced proliferation of neural progenitor cells (NPC) in the adult DG. Using bromodeoxyuridine incorporation assays, we showed that the number of mature newborn granule neurons generated de novo was similar in both wild-type (WT) and Cdk2-deficient adult mice. Moreover, the apparent lack of cell output reduction in Cdk2(-/-) mice DG did not result from a reduction in apoptosis of newborn granule cells as analyzed by TUNEL assays. Our results therefore suggest that Cdk2 is dispensable for NPC proliferation, differentiation and survival of adult-born DG granule neurons in vivo. These data emphasize that functional redundancies between Cdks also occur in the adult brain at the level of neural progenitor cell cycle regulation during hippocampal neurogenesis. [less ▲]

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See detailCdk2 loss accelerates precursor differentiation and remyelination in the adult central nervous system.
Caillava, Céline; Vandenbosch, Renaud ULg; Jablonska, Beata et al

in Journal of Cell Biology (2011), 193(2), 397-407

The specific functions of intrinsic regulators of oligodendrocyte progenitor cell (OPC) division are poorly understood. Type 2 cyclin-dependent kinase (Cdk2) controls cell cycle progression of OPCs, but ... [more ▼]

The specific functions of intrinsic regulators of oligodendrocyte progenitor cell (OPC) division are poorly understood. Type 2 cyclin-dependent kinase (Cdk2) controls cell cycle progression of OPCs, but whether it acts during myelination and repair of demyelinating lesions remains unexplored. Here, we took advantage of a viable Cdk2(-/-) mutant mouse to investigate the function of this cell cycle regulator in OPC proliferation and differentiation in normal and pathological conditions. During central nervous system (CNS) development, Cdk2 loss does not affect OPC cell cycle, oligodendrocyte cell numbers, or myelination. However, in response to CNS demyelination, it clearly alters adult OPC renewal, cell cycle exit, and differentiation. Importantly, Cdk2 loss accelerates CNS remyelination of demyelinated axons. Thus, Cdk2 is dispensable for myelination but is important for adult OPC renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin. [less ▲]

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See detailCdk6-dependent regulation of g(1) length controls adult neurogenesis.
Beukelaers, Pierre; Vandenbosch, Renaud ULg; Caron, Nicolas ULg et al

in Stem Cells (2011), 29(4), 713-24

The presence of neurogenic precursors in the adult mammalian brain is now widely accepted, but the mechanisms coupling their proliferation with the onset of neuronal differentiation remain unknown. Here ... [more ▼]

The presence of neurogenic precursors in the adult mammalian brain is now widely accepted, but the mechanisms coupling their proliferation with the onset of neuronal differentiation remain unknown. Here, we unravel the major contribution of the G(1) regulator cyclin-dependent kinase 6 (Cdk6) to adult neurogenesis. We found that Cdk6 was essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Specifically, Cdk6 deficiency prevents the expansion of neuronally committed precursors by lengthening G(1) phase duration, reducing concomitantly the production of newborn neurons. Altogether, our data support G(1) length as an essential regulator of the switch between proliferation and neuronal differentiation in the adult brain and Cdk6 as one intrinsic key molecular regulator of this process. STEM Cells 2011;29:713-724. [less ▲]

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See detailcDNA cloning and expression of bovine procollagen I N-proteinase: a new member of the superfamily of zinc-metalloproteinases with binding sites for cells and other matrix components.
Colige, Alain ULg; Li, S. W.; Sieron, A. L. et al

in Proceedings of the National Academy of Sciences of the United States of America (1997), 94(6), 2374-9

Procollagen N-proteinase (EC 3.4.24.14) cleaves the amino-propeptides in the processing of type I and type II procollagens to collagens. Deficiencies of the enzyme cause dermatosparaxis in cattle and ... [more ▼]

Procollagen N-proteinase (EC 3.4.24.14) cleaves the amino-propeptides in the processing of type I and type II procollagens to collagens. Deficiencies of the enzyme cause dermatosparaxis in cattle and sheep, and they cause type VIIC Ehlers-Danlos syndrome in humans, heritable disorders characterized by accumulation of pNcollagen and severe skin fragility. Amino acid sequences for the N-proteinase were used to obtain cDNAs from bovine skin. Three overlapping cDNAs had an ORF coding for a protein of 1205 residues. Mammalian cells stably transfected with a complete cDNA secreted an active recombinant enzyme that specifically cleaved type I procollagen. The protein contained zinc-binding sequences of the clan MB of metallopeptidases that includes procollagen C-proteinase/BMP-1. The protein also contained four repeats that are homologous to domains found in thrombospondins and in properdin and that can participate in complex intermolecular interactions such as activation of latent forms of transforming growth factor beta or the binding to sulfatides. Therefore, the enzyme may play a role in development that is independent of its role in collagen biosynthesis. This hypothesis was supported by the observation that in some tissues the levels of mRNA for the enzyme are disproportionately high relative to the apparent rate of collagen biosynthesis. [less ▲]

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See detailcDNA-AFLP analysis of Candida oleophila (strain O) genes differentially expressed during the biocontrol of Botrytis cinerea on harvested apples
Bajji, Mohammed; Jijakli, Haissam ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), 43

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See detailcDNA-AFLP analysis of gene expression changes in apple trees induced by phytoplasma infection during compatible interaction
Aldaghi, Majid; Bertaccini, Assunta; Lepoivre, Philippe ULg

in European Journal of Plant Pathology (2012), 134

Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment ... [more ▼]

Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique was used. A rootstock of apple (MM106) susceptible to ‘Ca. P. mali’ was used to extend the range of the potential host responses by the maximum number of identified genes that will be deregulated by phytoplasma in apple. Gene expression comparisons were studied in three directions: healthy versus infected samples, symptomatic versus nonsymptomatic sample, and AP-infected versus ATinfected sample. Forty-five genes whose steady-state levels of expression significantly changed in response to phytoplasma infection were identified. Among their partial cDNA sequences, only 27 showed similarity to DNA or protein data bases; of these, 18 were related to known genes in plants, and the rest were related to unknown or hypothetical proteins. Eighteen out of 45 did not show any similarity with sequences in data bases (potential novel genes). Quantitative real-time RT-PCR (qRT-PCR) was used to confirm differential expression of AFLP identified genes, and showed the similar profile expression for 11 known genes among 18, and for 13 unknown, hypothetical or novel genes among 27. Changes in gene expression involved a wide spectrum of biological functions, including processes of metabolism, cell defence, senescence, photosynthesis, transport, transcription, signal transduction and protein synthesis. This is the first study of global gene profiling in plants in response to phytoplasma infections using cDNA-AFLP, and a model is proposed to explain the mode of action of the ‘Ca. P. mali’ in apple. [less ▲]

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See detailCDO Prices and Risk Management: A Comparative Study of Alternative Approaches for pricing iTraxx
Bourdoux, Jean-Michel; Hübner, Georges ULg; Sibille, Jean-Roch ULg

in Wagner, Niklas (Ed.) Credit Risk - Models, Derivatives and Management (2008)

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See detailCDS and Financial Stability
Sougné, Danielle ULg; Mattar, Jamal ULg

Conference (2012, September 20)

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See detailCDS and financial stability
Sougné, Danielle ULg; Mattar, Jamal ULg

Conference (2012, August 01)

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See detailCDS Special Publication no 10. IDAAS 1988
Heck, A.; Manfroid, Jean ULg

in Bulletin d'Information du Centre de Données Stellaires (1987), 33

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See detailCDS Special Publication n° 9: International Directory of Professional Astronomical Institutions 1987
Heck, A.; Manfroid, Jean ULg

in Bulletin d'Information du Centre de Données Stellaires (1986), 31

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See detailCdTe Nanocrystals: Dynamic Effect of Solvation on the Optical Properties of a CdTe Nanocrystal (Advanced Optical Materials 3/2013)
Malcioǧlu, O. B.; Raty, Jean-Yves ULg

in Advanced Optical Materials (2013), 1(3), 238-238

Molecular dynamics simulations are used to establish a correlation between the time evolution of spectral features with various structural components of a ligand-stabilized CdTe quantum dot. The ... [more ▼]

Molecular dynamics simulations are used to establish a correlation between the time evolution of spectral features with various structural components of a ligand-stabilized CdTe quantum dot. The localization of charge oscillations due to light absorption is investigated by O. B. Malci{dotless}oǧlu et al. on page 239 before and after embedding the quantum dot in an explicit solvent environment. The image shows a snapshot from the solvated molecular dynamics trajectory. The response charge density of a quantum dot to two different frequencies of irradiation is depicted as colored glass bubbles. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. [less ▲]

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See detailCe cavernome cérébral est-il la cause d'un syndrome parkinsonien ?
Benmouna, Karim ULg; DIVE, Dominique ULg; WANG, François-Charles ULg et al

in Revue Médicale de Liège (2013), 68(12), 613-616

We report the case of a patient presenting with an akineto-rigid syndrome of the left hemibody whose etiological exploration by magnetic resonance imaging showed the presence of a cavernoma located in the ... [more ▼]

We report the case of a patient presenting with an akineto-rigid syndrome of the left hemibody whose etiological exploration by magnetic resonance imaging showed the presence of a cavernoma located in the right lenticular region. The interest of this situation lies in establishing whether pathophysiological link may exist between such symptoms and the lesion. [less ▲]

Detailed reference viewed: 205 (40 ULg)