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See detailCaractéristiques spermatiques des taureaux de race Borgou au Bénin
Adamou N'Diaye, M.; Gbangboche, A.B.; Adjovi, A. et al

in Annales des Sciences Agronomiques du Bénin (2000), 2

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See detailCaractéristiques techniques et aspects environnementaux de l’élevage de porcs charcutiers et de porcelets sevrés sur litières accumulées
Nicks, Baudouin ULg

in Annales de Médecine Vétérinaire (2004), 148(1), 31-38

The technique of raising pigs on deep litter consists of leaving the animals on a 30 to 50 cm depth of straw or sawdust which can be used for several batches. This article gives a synthesis on ... [more ▼]

The technique of raising pigs on deep litter consists of leaving the animals on a 30 to 50 cm depth of straw or sawdust which can be used for several batches. This article gives a synthesis on experimental results on rearing 3 to 4 successive batches of fattening pigs or 5 to 6 batches of weaned pigs on straw-based or sawdust-based deep litters. For fattening pigs, this rearing technique required on average 80 kg of sawdust or 45 kg of straw per pig and produced, on average, 123 kg per pig of sawdust-based compost or 159 kg per pig of straw-based manure with a nitrogen content of 1.29 kg and 1.87 kg respectively. For weaned pigs, the rearing technique required on average 15 kg of sawdust or 6 kg of straw per pig and produced, on average, 17 kg of sawdust-based compost per pig or an equivalent quantity of straw-based manure with a nitrogen content of 177 g and 210 g respectively. The cumulative greenhouse gases emissions (CO2, CH4, N2O), calculated in CO2 equivalents, were higher with sawdust-based litter than with straw-based litter of 42 % during fattening periods and of 53% during post-weaning periods. On the contrary, NH3 emissions were higher from the strawbased litter than from the sawdust-based litter but only during post- weaning periods (+ 160 %), not during fattening periods. None of the two litters presents a decisive advantage on the other on an environmental point of view. [less ▲]

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See detailCaractéristiques ultrastructurales des nucléoles de cellules HEp 2
Thiry, Marc ULg

in Biology of the Cell (1985), 53

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See detailCaracterización de cepas de Escherichia coli productoras de toxina Shiga aisladas de bovinos: presencia de marcadores de virulencia atípicos
Larzábal, M.; Vilte, D.; Rodriguez, Sabrina ULg et al

Poster (2006, May 10)

CARACTERIZACIÓN DE CEPAS DE Escherichia coli PRODUCTORAS DE TOXINA SHIGA AISLADAS DE BOVINOS: PRESENCIA DE MARCADORES DE VIRULENCIA ATÍPICOS. Larzábal M. (1); Vilte D.A. (1); Rodríguez S. (1); Elizondo A ... [more ▼]

CARACTERIZACIÓN DE CEPAS DE Escherichia coli PRODUCTORAS DE TOXINA SHIGA AISLADAS DE BOVINOS: PRESENCIA DE MARCADORES DE VIRULENCIA ATÍPICOS. Larzábal M. (1); Vilte D.A. (1); Rodríguez S. (1); Elizondo A.M. (1); Irino K. (2); Mercado E.C. (1) (1) Instituto de Patobiología, CICVyA, CNIA, Instituto Nacional de Tecnología Agropecuaria (INTA). CC25 (1712), Castelar, Prov. Buenos Aires, Argentina. (2) Departamento de Microbiología, Sección Enterobacterias, Instituto Adolfo Lutz, SP, Brasil. emercado@cicv.inta.gov.ar Palabras Clave: STEC, bovinos, marcadores virulencia. Introducción: Escherichia coli productor de toxina Shiga (STEC) produce enfermedad severa en el hombre, cuyo espectro clínico incluye diarrea, colitis hemorrágica (CH) y síndrome urémico hemolítico (SUH), la principal causa de falla renal aguda en niños. STEC es considerado un agente zoonótico, siendo el ganado bovino el principal reservorio de infección para el hombre. Factores de virulencia adicionales, como la producción de intimina (eae) y enterohemolisina (ehxA) se encuentran presentes en los serotipos de STEC denominados enterohemorrágicos (EHEC). Objetivo: caracterizar los marcadores de virulencia propios de EHEC e investigar la presencia de factores de virulencia putativos en cepas STEC. Materiales y Métodos: 35 cepas STEC aisladas de heces de terneros diarreicos (n=27) o sanos (n=3) y de carne bovina (n=5). Los subtipos de Stx2 (stx2) e intimina (eae) fueron determinados mediante PCR-RFLP. Genes codificantes de toxinas (ehxA, cdt-III, cdt-IV, cnf1, cnf2 y astA), adhesinas (afaE8, f17A, sfa, pap y clpG) y aerobactina (iucD) fueron detectados mediante PCR. La identificación de los antígenos O y H se llevó a cabo mediante métodos standard. Resultados: la mayoría de las cepas (n=27) perteneció a seropatotipos asociados con frecuencia a CH y SUH. Los serotipos O157 (n=6), O5:H- (n=5), O26:H- (n=1),O26:H11 (n=6), O111:H- (n=8) y O145:H- (n=1), los subtipos de toxina Stx2 (n=11), Stx2vh-b (n=2) y Stx2vh-a (n=1), y los genes eae (n=32) y ehxA (n=35) identificados, son marcadores de virulencia de cepas altamente patógenas para el hombre. Algunas de estas cepas presentaron marcadores de virulencia propios de E. coli diarragénicos (astA) o productores de infecciones extraintestinales (ExPEC) (cdt-III, iucD, f17A, pap). Marcadores característicos de cepas de origen bovino (cnf2, afaE8) se detectaron en serotipos poco frecuentes en humanos. Conclusiones: el ganado bovino es reservorio de cepas EHEC altamente virulentas. La función en cepas EHEC de factores de virulencia característicos de cepas ExPEC debe ser investigada. Algunos de los genes detectados podrían ser parte de islas de patogenicidad parcialmente deleteadas presentes en el cromosoma de cepas EHEC o pertenecer a elementos genéticos móviles recientemente adquiridos. [less ▲]

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See detailCaracterización de Escherichia coli enterohemorrágico aislado de terneros diarreicos.
Mercado, E.C.; Rodriguez, Sabrina ULg; Gioffré, A. et al

Poster (2001, October 07)

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See detailCaracterización de los factores implicados en la resistencia/sensibilidad a la infección del virus influenza en modelos murinos Mx-negativos
Casanova Bustos, Tomas Ronaldo ULg; Daniel, Desmecht; Mutien, Garigliany

Poster (2013, June)

DBA/2J mice and C57BL/6J are two extremes in terms of susceptibility to influenza A virus among Mxnegative mouse strains. Several research teams focused on the factors explaining this difference, mainly ... [more ▼]

DBA/2J mice and C57BL/6J are two extremes in terms of susceptibility to influenza A virus among Mxnegative mouse strains. Several research teams focused on the factors explaining this difference, mainly by genetic approaches using Recombinant Inbred Lines between those two strains. Several candidate-genes have been proposed, but it was not possible to determine their importance. We chose a phenotypic approach, by dissecting each stage of influenza A infection virus in mice of each line, aiming at identifying critical differences between C57BL/6J and DBA/2J. Preliminary observations suggest that either the viral infection of the airway epithelium of DBA/2J is more productive, either alveolar macrophages from C57BL/6J are more efficient in viral particles phagocytosis, or a combination of these two mechanisms. We isolated and cultured tracheal cells, pneumocytes and alveolar macrophages from both strains of mice to determine the permissiveness of the cells of the respiratory tree, quantify the specific receptors of influenza A virus and to compare the alveolar macrophage phagocytic abilities. We have demonstrated a greater presence of α2,3 receptors on alveolar macrophages and tracheal cells of DBA/2J and a higher in vitro viral amplification on DBA/2J respiratory cells. [less ▲]

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See detailCaracterizacion del comportamiento elasto-plastico de placas metalicas
Flores, Paulo; Habraken, Anne ULg

(2006)

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See detailCaracterización del producto cerámico en las comunidades neolíticas de mediados del V milenio Cal BC: El valle del Éufrates y el valle del Khabur en el Halaf final (Siria).
Gomez Bach, Ana Maria ULg

Doctoral thesis (2011)

The characterization of pottery production in the mid-sixt millennium cal BC in Neolithic communities: the Euphrates valley and Khabur basin in Late Halaf. SUMMARY The characterization of pottery ... [more ▼]

The characterization of pottery production in the mid-sixt millennium cal BC in Neolithic communities: the Euphrates valley and Khabur basin in Late Halaf. SUMMARY The characterization of pottery production in the mid-sixth millennium cal BC contexts in the Middle East arises from the need to understand how consolidated agricultural and pastoral communities of the Fertile Crescent were structured and organized. This chrono cultural period is embodied in an archaeological record of great interest in order to identify the different economic, social and symbolic responses at the final moment of the so-called Halaf culture. The opportunity to study the pottery assemblages from two sites and two regions, such as the Euphrates valley and the valley of Khabur, with new material from Tell Halula (16,668 sherds) and Chagar Bazar (21,194 sherds), allowed us to study and apply the same methodology to both sets from the very beginning of fieldwork. The analysis of 37,882 fragments from a comprehensive perspective and by using archaeometric techniques (chemical, petrographic and PIXE analysis) as well as morphometric and basic techno functional characterization analysis set new guidelines on the archaeological potential of these sets. Drawn up from Archaeology and Materials Science, this contribution has its methodological framework focused on rebuilding the chaîne operatoire. This reconstruction has allowed us to study and isolate two assemblages and to outline the existence of different socio economic practices, some of which had clear regional links and a strong substrate, but above all with basic subsistence practices which can be addressed from the ceramic product itself. Likewise, the specific chronological framework provided by new radiocarbon dates of both sites, which are between 5600-5300 cal BC, enables not only to characterise these productions but also to arise new questions to understand the interaction mechanisms between communities; mechanisms that cover from basic subsistence practices, i.e. the handling and processing of food and products, to mechanisms which regulate and structure the group. [less ▲]

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See detailCaracterización funcional de Snail2 como represor de cadherina- E y como regulador del proceso de carcinogenésis química
Molina Ortiz, Patricia ULg

Doctoral thesis (2010)

The loss of expression of intercellular adhesion protein E-cadherin is a key event in the process of epithelial-mesenchymal transition (EMT), which allows cell migration during embryonic development and ... [more ▼]

The loss of expression of intercellular adhesion protein E-cadherin is a key event in the process of epithelial-mesenchymal transition (EMT), which allows cell migration during embryonic development and invasion of tumor cells of epithelial origin (carcinomas). The transcriptional repression is one of the main mechanisms of negative regulation of E-cadherin expression in both embryonic development and during tumor progression. In recent years there has been identified different transcriptional repressors of E-cadherin gene, which in turn induce TEM, including factors the zinc finger family of Snail (Snail1 and Snail2) and ZEB (ZEB - 1 and ZEB-2) and factors of the bHLH family (E47 and Twist). Previous studies of our laboratory, comparing gene expression profiles analysis of MDCK-II cells with stable expression of Snail1, Snail2 or E47 identified different genetic programs induced by each of these factors. These differences indicate that factors Snail1, Snail2 and E47 are regulating specific genetic programs that can modify the invasive capacity, migratory, proliferative and angiogenic capacity of cells that overexpress each of these factors. These results are consistent with the functional differences between these factors during embryonic development and tumor progression. This thesis analyzed the transcriptional mechanisms used by Snail2 for transcriptional repression of E-cadherin and its role in the induction of carcinomas in mice. Biochemical studies showed that Snail2 required the SNAG (Snail common domain and Gfi-1) and SLUG domains for the recruitment of different co-repressors, such as mSin3A, NCoR and CtBP1, and that these co-repressors functionally interact during transcriptional repression -E-cadherin. We have also identified in Snail2 different phosphorylated residues in vivo that can regulate the activity of Snail2. Finally, functional analysis of zinc fingers revealed that while the second zinc finger is essential for the repression activity of Snail2, the fifth zinc finger has a promoter context-dependent function, by altering the Snail2 capacity of repression dependent of the promoter. These results highlight differences and similarities between the mechanisms of transcriptional repression of Snail and Snail2, supporting the functional differences between the two. Animal model studies showed that Snail2 is essential for the survival and differentiation of keratinocytes. However, the absence of Snail2 increases the occurrence and malignant progression of carcinomas in the chemical carcinogenesis model in mouse skin. The anti-inflammatory treatment reduces malignant progression but not the emergence and growth of benign lesions. These results indicate that the absence of Snail2 during carcinogenesis promotes inflammatory processes that alter the terminal differentiation and proliferation capacity of keratinocytes by promoting carcinogenesis. [less ▲]

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See detailCaracterización y cuantificación autmatizadas de menas metálicas mediante visión artificial : proyecto cameva
Castroviejo, Ricardo; Catalina, Juan-Carlos; Bernhardt, Heinz-Juergen et al

in Actos del XIII Congreso Latino Americano de Geologia (2008)

The CAMEVA project (Automated system for the identification and quantitative measurement of ore minerals) aims to develop an automated system able to perform the identification and quantification of the ... [more ▼]

The CAMEVA project (Automated system for the identification and quantitative measurement of ore minerals) aims to develop an automated system able to perform the identification and quantification of the different minerals present in a sample of metallic ores, in order to facilitate their industrial use. The system comprises a motorised reflected light microscope, a filter wheel (monochromator), a research BW camera, and a computer running a digital image analysis software associated to an expert system specially developed for this application. Multispectral reflectance values are measured for visible light and near IR and UV (350-1000 nm), and no polarisation is usually employed in order to reduce measurement variability due to mineral anisotropy. Mineral identification is carried out by comparison of measured reflectances with spectral reflectance databases (e.g. IMA-COM). Previous experiences showed that the most common ores can be differentiated through the analysis of a microscopic RGB colour image, by constraining the search with additional information such as the type of deposit and the corresponding mineral association. The project goes further, and intends to identify most of the ores from multi-spectral images, including non-visible bands, resorting to an expert system specifically built to deal with the different types of information available (spectral reflectance, sample origin, mineral associations, morphology, etc). 2 Validation of the method will be carried out by comparative análisis of standards and samples exchanged between the laboratories. Development pays special attention to the total automation of the process (including calibration) and to the self-learning capabilities of the system. Contacts with research institutions and with the industry will allow to apply the system to real problems. The system can be used to control the processing of ores as well as to prevent pollution for the protection of the environment (e.g. sulphides in coal or in industrial tailings). Further applications can be envisaged in the future: e.g. other minerals, coal or materials, particles, etc. [less ▲]

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See detailCaracterización y estudio de inversión para la reconstrucción de los sistemas de riego canillá, Rio Blanco, Tzununul, Chuaxic y Xibalbay
Estrada Orozco, Nick Kenner ULg

Report (2014)

Las unidades de riego en Guatemala que en total suman 29 han sido construidas en promedio desde hace mas de 25 años, la vida util de las mismas a llegado a su fin ademas de ser vistimas de fenomenos ... [more ▼]

Las unidades de riego en Guatemala que en total suman 29 han sido construidas en promedio desde hace mas de 25 años, la vida util de las mismas a llegado a su fin ademas de ser vistimas de fenomenos naturales que han en algunos destruido gran parte de la infraestructura hidraulica, por tal motivo el ministerio de agricultura ha decidido realizar una serie de estudios que permitan cuantificar la inversión necesaria para que estos sistemas de riego puedan operar a un ciento por ciento. Parte de estos estudios se realizo este, que comprende las unidaades de riego de Chuaxic, Xibalbay, Canilla, Rio blanco y Tzununul de los departamentos de Solola y Quiche, para determinar los tipos de daños y la inversion requerida para su reconstruccion. [less ▲]

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See detailCaracterization del comportamiento mecanico de aceros para embuticion profunda
GARCIA, C.; CELENTANO, D.; Ponthot, Jean-Philippe ULg

in Proceedings of the joint conference Jornadas SAM, el Congreso Nacional de Metalurgia y Materiales de Chile (2003)

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See detailCaracterization del jambon belga de las ardenas.
Hannecart, François; Deroanne, Claude; Sindic, Marianne ULg

in Abstracts du 3ème Congrès mondial du jambon sec (2005, May)

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See detailCARAIB - A global model of terrestrial biological productivity
Warnant, Pierre ULg; François, Louis ULg; Strivay, David ULg et al

in Global Biogeochemical Cycles (1994), 8(3), 255-270

CARAIB, a mechanistic model of carbon assimilation in the biosphere estimates the net primary productivity (NPP) of the continental vegetation on a grid of 1 degrees x 1 degrees in latitude and longitude ... [more ▼]

CARAIB, a mechanistic model of carbon assimilation in the biosphere estimates the net primary productivity (NPP) of the continental vegetation on a grid of 1 degrees x 1 degrees in latitude and longitude. The model considers the annual and diurnal cycles. It is based on the coupling of the three following submodels; a leaf assimilation model including estimates of stomatal conductance and leaf respiration, a canopy model describing principally the radiative transfer through the foliage, and a wood respiration model. Present-day climate and vegetation characteristics allow the discrimination between ecotypes. In particular, specific information on vegetation distribution and properties is successfully used at four levels; the leaf physiological level, the plant level, the ecosystem level, and the global level. The productivity determined by the CARAIB model is compared with local measurements and empirical estimates showing a good agreement with a global value of 65 Gt C yr(-1). The sensitivity of the model to the diurnal cycle and to the abundance of C-4 species is also tested. The productivity slightly decreases (10%) when the diurnal cycle of the temperature is neglected. By contrast, neglecting the diurnal cycle of solar irradiance produces unrealistically high values of NPP. Even if the importance of this increase would presumably be reduced by the coupling of CARAIB with a nutrient cycle model, this test emphasizes the key role of the diurnal cycle in a mechanistic model of the NPP. Uncertainties on the abundance and spatial distribution of C-4 plants may cause errors in the NPP estimates, however, as demonstrated by two sensitivity tests, these errors are certainly lower than 10% at the global scale as shown by two tests. [less ▲]

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See detailCARAIB USER'S GUIDE
Minet, Julien ULg; Jacquemin, Ingrid ULg; François, Louis ULg

Learning material (2013)

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See detailCARAIB: A GLOBAL MECHANISTIC VEGETATION MODEL TO STUDY THE CO2 EXCHANGE BETWEEN THE ATMOSPHERE AND THE CONTINENTAL BIOSPHERE
Gérard, Jean-Claude ULg; François, Louis ULg; Nemry, Beranrd et al

Conference (1996, September)

The TM2 atmospheric transport model, a parameterization of soil heterotrophic respiration and a mechanistic model of carbon assimilation in the biosphere (CARAIB) are combined to estimate the net primary ... [more ▼]

The TM2 atmospheric transport model, a parameterization of soil heterotrophic respiration and a mechanistic model of carbon assimilation in the biosphere (CARAIB) are combined to estimate the net primary productivity of the continental vegetation. The model includes the determination of the leaf area index (LAI) of the various vegetation covers, the CO2 assimilation by the leaves, and the respiration of standing biomass. It also includes a soil hydrological model forced with monthly mean air temperature, precipitation and solar radiation fields and using a stochastic generation of daily weather within each month. It is shown that the seasonal fluctuations observed at various monitoring stations can be well reproduced, thus validating the mechanistic approach adopted to calculate the global net primary productivity. A Fourier analysis of the modelled atmospheric signal is performed to quantify the relative importance of the vegetation in the different latitude zones and the contribution of the various vegetation types. In the southern hemisphere, the calculated phase and amplitude of the CO2 signal are not so well reproduced since atmosphere-ocean exchanges should also be considered in addition to the biospheric source. The latest updates and simulations performed with the model will be presented and discussed. [less ▲]

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