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See detailCloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10
Arpigny, J. L.; Feller, Georges ULg; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1993), 1171(3), 331-3

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M ... [more ▼]

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold. [less ▲]

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See detailCloning, Sequencing and Characterization of PIT1 Gene in Ovis aries
Bastos, Estella; Santos, Ingrid; Parmentier Isabelle et al

in Genetica (2006), 126

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See detailCloning, sequencing and overexpression in Escherichia coli of the alginate lyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases
Chavagnat, Frederic; Duez, Colette ULg; Guinand, Micheline et al

in Biochemical Journal (1996), 319(Pt 2), 575-583

A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form ... [more ▼]

A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form of a 233-amino-acid precursor. P. alginovora Aly has been overproduced in E. coli with a His-tag sequence fused at the C-terminal end under conditions in which the formation of inclusion bodies is avoided. His-tagged P. alginovora Aly has the same enzymic properties as the wild-type enzyme and has the specificity of a mannuronate lyase. It can be purified in a one-step procedure by affinity chromatography on Ni(2+)-nitriloacetate resin. The yield is of 5 mg of enzyme per litre of culture. The amplification factor is 12.5 compared with the level of production by wild-type P. alginovora. The six alginate lyases of known primary structure fall into three distinct classes, one of which comprises the pair P. alginovora Aly and Klebsiella pneumoniae Aly. [less ▲]

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See detailClONO2 total vertical column abundances above the Jungfraujoch Station, 1986-1994: Long-term trend and winter-spring enhancements
Rinsland, C. P.; Zander, Rodolphe ULg; Demoulin, Philippe ULg et al

in Journal of Geophysical Research (1996), 101(D2), 3891-3899

Total vertical column abundances of chlorine nitrate (ClONO2) have been retrieved from 0.006 cm(-1) resolution solar absorption spectra recorded at the International Scientific Station of the Jungfraujoch ... [more ▼]

Total vertical column abundances of chlorine nitrate (ClONO2) have been retrieved from 0.006 cm(-1) resolution solar absorption spectra recorded at the International Scientific Station of the Jungfraujoch (ISSJ) in the Swiss Alps (altitude 3.58 km, latitude 46.5 degrees N, longitude 8.0 degrees E) on 105 days between June 1986 and November 1994. The analysis is based on spectral fittings of the ClONO2 nu(4) band Q branch at 780.21 cm(-1) and the interferences occurring in the same spectral region. The ISSJ measurements show a regular long-term increase in the ClONO2 column with an occasional factor of 2 to 3 enhancements during the midwinter to early spring. Excluding data from this time of the year, the ISSJ database reflects a linear rate of increase and la uncertainty equal to (4.0 +/- 0.7)% yr(-1) referenced to 1990.0. The corresponding ClONO2 total vertical columns for mid-1986 and mid-1994 are equal to 0.92 and 1.26 x 10(15) molecules cm(-2), respectively. The high ClONO2 columns and high HF/HCl column ratios sometimes measured during winter indicate the occasional presence of chemically processed air above the station. This is corroborated by trajectories calculated for the stratospheric air masses sounded on these occasions. [less ▲]

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See detailClosantel Intoxication in a Dog
Mc Entee, Kathleen ULg; Grauwels, Magda ULg; Clercx, Cécile ULg et al

in Veterinary and Human Toxicology (1995), 37(3), 234-6

A case of overdosage with closantel, a salicynalide derivative, in a dog is described. The dog received 6 times the recommended dosage. Closantel induced optic neuritis, retinal degeneration, partial ... [more ▼]

A case of overdosage with closantel, a salicynalide derivative, in a dog is described. The dog received 6 times the recommended dosage. Closantel induced optic neuritis, retinal degeneration, partial deafness, hepatotoxicosis and myopathy. Only the blindness was irreversible. The therapy included albumin administration to reduce the acute toxicity of closantel. [less ▲]

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See detailA close look at the RGS spectra of the O4Ief star Zeta Pup
Rauw, Grégor ULg; Flores, A.; Nazé, Yaël ULg et al

Poster (2010)

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See detailClose projected QSO-Galaxy associations: are they real?
Claeskens, Jean-François ULg; Surdej, Jean ULg

in Astronomy and Astrophysics (1998), 335

The present status of close projected associations between high redshift quasars and foreground galaxies is reviewed in the framework of gravitational lensing, adopting both an observational and a ... [more ▼]

The present status of close projected associations between high redshift quasars and foreground galaxies is reviewed in the framework of gravitational lensing, adopting both an observational and a theoretical approach. The Non Singular Isothermal Spherical lens model (Hinshaw & Krauss 1987) is used. We confirm that a stronger overdensity q of bright galaxies is expected at small projected angular separations (<= 3arcsec ) from high redshift and bright (thus highly luminous) quasars (HLQs). The effects of a non singular galactic core radius and/or microlensing only slightly enhance this expected overdensity. The predictions reproduce pretty well the scarce observations which are presently available, with the exception of two rather high overdensities previously claimed in the literature (e.g. q=2.9 for theta <= 6arcsec , Webster & Hewett 1990). Uncontrolled morphological selections may affect these controversial results so that additional unbiased observations are badly needed before rejecting gravitational lensing as the mechanism to produce the observed close angular QSO-galaxy associations. We describe three new selected and bias-free samples consisting of a total of 219 different HLQs. Although an apparent overdensity is detected in two of them, these galaxy excesses are found to be statistically not significant. Firm confirmation of the reality of close projected QSO-Galaxy associations is hampered by small number statistics: the expected and observed absolute numbers of such QSO-galaxy associations are still presently found to be very small. Therefore, the corresponding error bars are quite large. We conclude that the best observational strategy to significantly detect (at 3sigma ) an overdensity of galaxies near HLQs is to observe about 1500 HLQs (M_V ~ -29) down to a limiting magnitude Rlim ~ 23. Future automated surveys may provide us with such a large and unbiased database. In conclusions, we find that the lensing-induced correlation between galaxies and HLQs at small angular separations consists of a very interesting but weak effect, much less sensitive to cosmological or astrophysical parameters than the number of multiply imaged sources expected within a sample of HLQs. Based on data collected at the European Southern Observatory, La Silla, Chile [less ▲]

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See detailClose-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors
Tourillon, Gérard; Dreesen, Laurent ULg; Volcke, Cédric et al

in Journal of Materials Science (2009), 44(24), 6805-6810

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy ... [more ▼]

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy performed in the Total Internal Reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. [less ▲]

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See detailClose-up of primary and secondary asteroseismic CoRoT targets and the ground-based follow-up observations
Uytterhoeven, K.; Poretti, E.; Rainer, M. et al

in Journal of Physics Conference Series (2008), 118

To optimise the science results of the asteroseismic part of the CoRoT satellite mission a complementary simultaneous ground-based observational campaign is organised for selected CoRoT targets. The ... [more ▼]

To optimise the science results of the asteroseismic part of the CoRoT satellite mission a complementary simultaneous ground-based observational campaign is organised for selected CoRoT targets. The observations include both high-resolution spectroscopic and multicolour photometric data. We present the preliminary results of the analysis of the ground-based observations of three targets. A line-profile analysis of 216 high-resolution FEROS spectra of the δ Sct star HD 50844 reveals more than ten pulsation frequencies in the frequency range 5-18 d[SUP]-1[/SUP], including possibly one radial fundamental mode (6.92 d[SUP]-1[/SUP]). Based on more than 600 multi-colour photometric datapoints of the β Cep star HD 180642, spanning about three years and obtained with different telescopes and different instruments, we confirm the presence of a dominant radial mode ν[SUB]1[/SUB] = 5.48695 d[SUP]-1[/SUP], and detect also its first two harmonics. We find evidence for a second mode ν[SUB]2[/SUB] = 0.3017 d[SUP]-1[/SUP], possibly a g-mode, and indications for two more frequencies in the 7-8 d[SUP]-1[/SUP] domain. From Stromgren photometry we find evidence for the hybrid 5 Sct/γ Dor character of the F0 star HD 44195, as frequencies near 3 d[SUP]-1[/SUP] and 21 d[SUP]-1[/SUP] are detected simultaneously in the different filters. [less ▲]

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See detailA closed form technique to predict springback in creep age-forming
Jeunechamps, Pierre-Paul ULg; Ho, K. C.; Lin, J. et al

in International Journal of Mechanical Sciences (2006), 48(6), 621-629

An efficient numerical method has been developed and programmed to predict springback in creep age-forming (CAF) of alummium shell components. Physically based unified creep-ageing constitutive equations ... [more ▼]

An efficient numerical method has been developed and programmed to predict springback in creep age-forming (CAF) of alummium shell components. Physically based unified creep-ageing constitutive equations determined for AA7010 at 150 degrees C are used to evaluate age-hardening and creep deformation in CAF processes. The studies are mainly concentrated on the pure bending of aluminium plates. Effects of plate thickness, single and double curvature bending, ageing time and stress relaxation features on springback are investigated. It is shown that the proposed algorithm can predict springback accurately and gives a relationship between geometrical data and springback. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailClosed-form dual perturb and combine for tree-based models
Geurts, Pierre ULg; Wehenkel, Louis ULg

in Proceedings of the International Conference on Machine Learning (ICML 2005) (2005)

This paper studies the aggregation of predictions made by tree-based models for several perturbed versions of the attribute vector of a test case. A closed-form approximation of this scheme combined with ... [more ▼]

This paper studies the aggregation of predictions made by tree-based models for several perturbed versions of the attribute vector of a test case. A closed-form approximation of this scheme combined with cross-validation to tune the level of perturbation is proposed. This yields soft-tree models in a parameter free way, and reserves their interpretability. Empirical evaluations, on classification and regression problems, show that accuracy and bias/variance tradeoff are improved significantly at the price of an acceptable computational overhead. The method is further compared and combined with tree bagging. [less ▲]

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See detailClosed-Loop Learning of Visual Control Policies
JODOGNE, Sébastien ULg

Doctoral thesis (2006)

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See detailClosed-Loop Learning of Visual Control Policies
Jodogne, Sébastien ULg; Piater, Justus ULg

in Journal of Artificial Intelligence Research (2007), 28

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See detailClosed-loop model of the cardiovascular system including ventricular interaction and valve dynamics: application to pulmonary embolism
Desaive, Thomas ULg; Dutron, S.; Lambermont, Bernard ULg et al

in 12th International Conference on Biomedical Engineering (2005)

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See detailClosed-loop transient stability emergency control
Ernst, Damien ULg; Pavella, Mania ULg

in Proceedings of the IEEE Power Engineering Society Winter Meeting 2000 (2000)

The question of transient stability control is revisited, various types of controls are identified, and a general approach to closed-loop emergency control is proposed. The focus is on feasibility aspects ... [more ▼]

The question of transient stability control is revisited, various types of controls are identified, and a general approach to closed-loop emergency control is proposed. The focus is on feasibility aspects, general salient features and illustration of stabilization capabilities of an emergency control scheme relying on generation shedding. [less ▲]

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See detailA closer eye on hedge funds' dynamic hedging
Babaei, Hamid; Muller, Aline ULg; Lambert, Marie ULg

Conference (2013, April 18)

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See detailA closer look at the low frequency dynamics of vortex matter
Raes, Bart; de Souza Silva, Clecio C.; Silhanek, Alejandro ULg et al

in Physical Review. B : Condensed Matter (2014), 90

Using scanning susceptibility microscopy, we shed new light on the dynamics of individual superconducting vortices and examine the hypotheses of the phenomenological models traditionally used to explain ... [more ▼]

Using scanning susceptibility microscopy, we shed new light on the dynamics of individual superconducting vortices and examine the hypotheses of the phenomenological models traditionally used to explain the macroscopic ac electromagnetic properties of superconductors. The measurements, carried out on a 2H-NbSe2 single crystal at relatively high temperature T = 6.8 K, show a linear amplitude dependence of the global ac-susceptibility for excitation amplitudes between 0.3 and 2.6 Oe. We observe that the low amplitude response, typically attributed to the oscillation of vortices in a potential well defi ned by a single, relaxing, Labusch constant, actually corresponds to strongly non-uniform vortex shaking. This is particularly pronounced in the fi eld-cooled disordered phase, which undergoes a dynamic reorganization above 0.8 Oe as evidenced by the healing of lattice defects and a more uniform oscillation of vortices. These observations are corroborated by molecular dynamics simulations when choosing the microscopic input parameters from the experiments. The theoretical simulations allow us to reconstruct the vortex trajectories providing deeper insight in the thermally induced hopping dynamics and the vortex lattice reordering. [less ▲]

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See detailClosing address for the session "Bullks and Characterization"
Vanderbemden, Philippe ULg

Conference (2010, November)

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See detailClostridia and enterotoxaemia
Mainil, Jacques ULg

Conference (2006)

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See detailClostridial brain abscess after glioblastoma resection: case report and critical review of the literature
Duntze, J; Litré, Claude Fabien ULg; Bajolet, O et al

in Neuro-Chirurgie (2009), 55(6), 569-72

Clostridium perfringens is rare in neurosurgery. The source of clostridial brain abscess is usually a penetrating head injury. We report the case of a 57-year-old man who had parietal glioblastoma ... [more ▼]

Clostridium perfringens is rare in neurosurgery. The source of clostridial brain abscess is usually a penetrating head injury. We report the case of a 57-year-old man who had parietal glioblastoma resection with local carmustine chemotherapy and who presented a clostridial brain abscess three weeks later. Progression was especially brutal, leading to patient's death in few hours. We discuss the etiology and progression of this case compared to the data reported in the literature. [less ▲]

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