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See detailThe Cold-Active M1 Aminopeptidase from the Arctic Bacterium Colwellia psychrerythraea
Bauvois, Cédric; Huston, Adrienne; Feller, Georges ULg

in Rawlings, Neil D.; Salvesen, Guy S. (Eds.) Handbook of Proteolytic Enzymes (Third Ed) (2013)

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See detailCold-Adapted Beta-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas Haloplanktis
Hoyoux, A.; Jennes, I.; Dubois, P. et al

in Applied and Environmental Microbiology (2001), 67(4), 1529-35

The beta-galactosidase from the Antarctic gram-negative bacterium Pseudoalteromonas haloplanktis TAE 79 was purified to homogeneity. The nucleotide sequence and the NH(2)-terminal amino acid sequence of ... [more ▼]

The beta-galactosidase from the Antarctic gram-negative bacterium Pseudoalteromonas haloplanktis TAE 79 was purified to homogeneity. The nucleotide sequence and the NH(2)-terminal amino acid sequence of the purified enzyme indicate that the beta-galactosidase subunit is composed of 1,038 amino acids with a calculated M(r) of 118,068. This beta-galactosidase shares structural properties with Escherichia coli beta-galactosidase (comparable subunit mass, 51% amino sequence identity, conservation of amino acid residues involved in catalysis, similar optimal pH value, and requirement for divalent metal ions) but is characterized by a higher catalytic efficiency on synthetic and natural substrates and by a shift of apparent optimum activity toward low temperatures and lower thermal stability. The enzyme also differs by a higher pI (7.8) and by specific thermodynamic activation parameters. P. haloplanktis beta-galactosidase was expressed in E. coli, and the recombinant enzyme displays properties identical to those of the wild-type enzyme. Heat-induced unfolding monitored by intrinsic fluorescence spectroscopy showed lower melting point values for both P. haloplanktis wild-type and recombinant beta-galactosidase compared to the mesophilic enzyme. Assays of lactose hydrolysis in milk demonstrate that P. haloplanktis beta-galactosidase can outperform the current commercial beta-galactosidase from Kluyveromyces marxianus var. lactis, suggesting that the cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants. [less ▲]

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See detailCold-Adapted Enzymes
Collins, T.; D'Amico, Salvino ULg; Marx, J. C. et al

in Gerday, Charles; Glansdorff, N. (Eds.) Physiology and biochemistry of extremophiles (2007)

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See detailCold-adapted enzymes
Georlette, D.; Bentahir, M.; Claverie, P. et al

in Bulte, J.; DeCuyper, M. (Eds.) Focus on Biotechnology – Physics and Chemistry Basis for Biotechnology (2001)

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See detailCold-adapted enzymes from marine antarctic microorganisms
Marx, J. C.; Collins, T.; D'Amico, Salvino ULg et al

in Marine Biotechnology (2007), 9(3, May-Jun), 293-304

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the ... [more ▼]

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the viscosity of the medium, which is also significantly enhanced by low temperatures, contributes to slow down reaction rates. This review analyses the different challenges and focuses on a key element of life at low temperatures: cold-adapted enzymes. The molecular characteristics of these enzymes are discussed as well as the adaptation strategies which can be inferred from the comparison of their properties and three-dimensional structures with those of their mesophilic counterparts. As these enzymes display a high specific activity at low and moderate temperatures associated with a relatively high thermosensitivity, the interest in these properties is discussed with regard to their current and possible applications in biotechnology. [less ▲]

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See detailCold-adapted enzymes: an unachieved symphony
D'Amico, Salvino ULg; Claverie, P.; Collins, T. et al

in Storey, K. B.; Storey, J. M. (Eds.) Cell and Molecular Responses to Stress vol.2. Protein adaptations and signal transduction, (2001)

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See detailCold-Adapted Enzymes: From Fundamentals to Biotechnology
Gerday, Charles ULg; Aittaleb, Mohamed; Bentahir, Mostafa et al

in Trends in Biotechnology (2000), 18(3), 103-7

Psychrophilic enzymes produced by cold-adapted microorganisms display a high catalytic efficiency and are most often, if not always, associated with high thermosensitivity. Using X-ray crystallography ... [more ▼]

Psychrophilic enzymes produced by cold-adapted microorganisms display a high catalytic efficiency and are most often, if not always, associated with high thermosensitivity. Using X-ray crystallography, these properties are beginning to become understood, and the rules governing their adaptation to cold appear to be relatively diverse. The application of these enzymes offers considerable potential to the biotechnology industry, for example, in the detergent and food industries, for the production of fine chemicals and in bioremediation processes. [less ▲]

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See detailCold-adapted microorganisms: adaptation strategies and biotechnological potential
Margesin, R.; Feller, Georges ULg; Gerday, Charles ULg et al

in Bitton, G. (Ed.) The Encyclopedia of Environmental Microbiology, vol. 2 (2002)

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See detailThe cold-adapted trigger factor from the Antarctic bacterium Pseudoalteromonas haloplanktis.
D'Amico, Salvino; Piette, Florence; Leprince, Pierre ULg et al

Poster (2008)

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See detailCold-adapted xylanases: from Antarctica to the table.
Collins, Tony; Georis, Jacques; Dauvrin, Thierry et al

Poster (2006)

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See detailDie "Colección de autores españoles" von F. A. Brockhaus (1860-1887): rentabler Konservatismus
Ceballos Viro, Alvaro ULg

in Witthaus, Jan-Henrik (Ed.) Beiträge zur Nationalisierung der Kultur im Spanien des aufgeklärten Absolutismus (2010)

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See detailColestasis asociada a ticlopidina
Diaz Fuenzalida, A.; VALDES SOCIN, Hernan Gonzalo ULg; Laudano, O. et al

in Gastroenterologia y Hepatologia (1997), 20(3), 128-30

The case of a 56-years-old male with cholestasis associated with ticlopidine is presented. Cholestasis is an infrequent adverse effect of this drug. The patient was admitted to hospital because of ... [more ▼]

The case of a 56-years-old male with cholestasis associated with ticlopidine is presented. Cholestasis is an infrequent adverse effect of this drug. The patient was admitted to hospital because of jaundice, choluria, and itching of one month of evolution. The patient had taken ticlopidine twice a day for 3 months up to one week prior to admission for peripheral arteriopathy. Biopsy was performed showing acinar cholestasis and portal inflammatory infiltrate compatible with cholestasis due to hypersensitivity. Ticlopidine was discontinued by the patient himself one week prior to admission. The drug was not readministered and the evolution of the clinical and biochemical parameters of cholestasis decreased. The patient was asymptomatic and laboratory data were normal 4 months later. [less ▲]

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See detailLa colibacillose aviaire
Stordeur, Philippe; Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2002), 146(1, FEB-MAR), 11-18

The avian pathogenic E. coli strains, although considered by almost like opportunist pathogen, represent actually one of the most important cause of economic losses in the poultry sector and is one of the ... [more ▼]

The avian pathogenic E. coli strains, although considered by almost like opportunist pathogen, represent actually one of the most important cause of economic losses in the poultry sector and is one of the most frequent cause of carcase rejection in the slaughter house. Colibacillosis, which the major way of penetration is the respiratory tract, affect essentially broilers and give variable lesions and manifestations in function of the age of the animals. Actually, some virulence factors have been studied and associated with avian pathogenic E. coli strains : P and F1 fimbriae, hemagglutination, serum resistance, aerobactin system. Recent studies have also showed that other virulence factors (F17 and Afa adhesins) were also present in the avian pathogenic E. coli, but their rule in the pathogenicity must to be established. Because of the diversity of virulence factors and the little of knowledge about us, no vaccine is actually available to protect efficacely poultries against colibacillosis. Thus, the antibiotherapy based on a good diagnostic and a good prevention remained the only things to do in order to overcome the disease, despite the augmentation of antibiotics resistance and the risk of transferring to humans. [less ▲]

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See detailLes colibacillose dans l'espèce bovine.
Mainil, Jacques ULg

Conference (1993)

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See detailLes colibacilloses dans l'espèce bovine
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (1993), 137

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See detailColite aigue revelant une maladie de Wegener
Lebas, Eglantine ULg; Gielen, Sabine ULg; Nguyen, Minh Ngoc ULg et al

in Revue Médicale de Liège (2006), 61(3), 163-8

We report the case of a 52 year old man who was hospitalized within a context of a persistent deterioration of his general condition. He was suspected of having a chronic inflammatory colitis. A pulmonary ... [more ▼]

We report the case of a 52 year old man who was hospitalized within a context of a persistent deterioration of his general condition. He was suspected of having a chronic inflammatory colitis. A pulmonary radiography revealed the presence of voluminous bilateral excavated masses with hydro-aerical levels. After having refuted among others a suspicion of tuberculosis, the results of a thoracic percutaneous transpleural lung aspiration by needle under tomodensitometric control steered our diagnosis towards a vascularitis of the Wegener disease type. A treatment with corticotherapy in large doses completed with cyclophosphamid allowed for clinical, biological and radiological improvement. Wegener's granulomatosis usually starts in an insidious manner with febrile episodes and an impairment of the general condition associated with inflammatory biological signs, as observed in our patient. After these warning symptoms, come ORL and/or pulmonary and/or renal impairment, which represent the classical triad of diffused GW. However a certain number of particularities unusual for that diagnosis characterized our patient and prompted the discussion of this case. [less ▲]

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See detailColl2-1, Coll2-1 NO2 and myeloperoxidase in OA patients before and after hip or knee replacement
Deberg, M; Labasse, A; Quettier, E et al

in ICRS news letter (2006)

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See detailColl2-1, Coll2-1 NO2 and myeloperoxydase in OA patients before and after hip or knee replacement
Deberg, M; Labasse, A; Quettier, E et al

in Osteoporosis International (2006)

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See detailColl2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis.
Verwilghen, Denis ULg; Martens, Ann; Busschers, Evita et al

in Veterinary Research Communications (2011), 35(7), 401-8

The measurement of biomarkers that reflect cartilage breakdown is a powerful tool for investigating joint damage caused by disease or injury. Particularly in cases of osteochondrosis, synovial ... [more ▼]

The measurement of biomarkers that reflect cartilage breakdown is a powerful tool for investigating joint damage caused by disease or injury. Particularly in cases of osteochondrosis, synovial concentrations of these biomarkers may reveal the presence of osteoarthritic changes. Coll2-1, Coll2-1 NO2 and myeloperoxidase have recently been introduced in equine osteoarticular research but comparison between the concentrations of these markers in OCD affected and healthy joints has not been made. Therefore, this study aimed at reporting the synovial concentrations of these biomarkers in joints affected with osteochondral fragments in the tarsocrural joint compared to unaffected joints. Myeloperoxidase and Coll2-1NO2 revealed to have similar levels between affected joints and controls. However, in contrast to previous studies using C2C the present study demonstrated that synovial levels of Coll2-1 were significantly elevated in tarsocrural joints affected with osteochondrosis. Thus, Coll2-1 may be an earlier marker of cartilage degeneration than other cartilage degradation markers that have been previously used in equine medicine. [less ▲]

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