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See detailCloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar
Montefusco-Siegmund, R. A.; Romero, A.; Kausel, G. et al

in Cell & Tissue Research (2006), 325(2), 277-285

In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from ... [more ▼]

In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from Atlantic salmon (Salmo salar) encoding C-RFa, a peptide structurally related to mammalian Prl-releasing peptide (PrRP). The deduced preprohormone precursor is composed of 155 amino acid residues presenting a 87.1% similarity to chum salmon C-RFa and a 100% similarity to all fish C-RFa in the bioactive precursor motifs. C-RFa-immunoreactive perikarya and fibres were located in the brain of S. salar, especially in the hypothalamus, olfactory tract, optic tectum and cerebellum. In contrast, immunolabelled fibres were not observed in the pituitary stalk or in the hypophysis. However, interestingly, we detected immunolabelled cells in the rostral pars distalis of the pituitary in the basolateral region in which Prl is synthesized. These results were confirmed by obtaining a strong signal by using reverse transcription/polymerase chain reaction (RTPCR) on mRNA from both hypothalamus and pituitary. These data show, for the first time, by immunohistochemistry and RT-PCR, that C-RFa is produced in pituitary cells. Finally, based on these results, a possible function for CRFa as a locally produced PrRP in this teleost is discussed. [less ▲]

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See detailCloning of the Rat Brain Cdna Encoding for the Slc-1 G Protein-Coupled Receptor Reveals the Presence of an Intron in the Gene
Lakaye, Bernard ULg; Minet, Arlette ULg; Zorzi, Willy ULg et al

in Biochimica et Biophysica Acta (1998), 1401(2), 216-20

In order to isolate new G protein-coupled receptors expressed in the cerebral cortex, a set of degenerate oligonucleotides corresponding to the third and seventh transmembrane segment were synthetized ... [more ▼]

In order to isolate new G protein-coupled receptors expressed in the cerebral cortex, a set of degenerate oligonucleotides corresponding to the third and seventh transmembrane segment were synthetized. Their use in PCR on rat brain cortex mRNA amplified several cDNA fragments. One of them, a 526 bp sequence, encoded for what was at that time an unknown G protein-coupled receptor. An oligonucleotide derived from the sequence was then used as a probe to isolate the receptor cDNA from a rat brain cDNA library. It encodes for a 353aa protein with seven transmembrane segments, three consensus N-glycosylation sites at the amino terminus and several potential phosphorylation sites in the intracellular loops. This protein shares 91% overall identity with a recently cloned human somatostatin-like receptor of 402aa named SLC-1. This suggests that we have cloned the rat orthologue of the human SLC-1. However, the extracellular N-terminus of the human receptor is 49 amino acids longer and shows 50% identity with the rat one. Because the human sequence was deduced from genomic DNA, we suspected the presence of an intron in the gene. This was confirmed by PCR using primers spanning the intron. On the basis of the sequence of a 128 kb fragment of chromosome 22 encompassing the SLC-1 gene, we were able to deduce a corrected amino acids sequence for the human receptor. So both rat and human SLC-1 receptors are 353aa long, with three consensus N-glycosylation sites. They share 96% identity at the amino acid level and are encoded by a gene containing one intron in the coding sequence. [less ▲]

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See detailCloning of the Saccharomyces cerevisiae proline permease structural gene PUR4 and ammonia-regulation in its expression
Jauniaux, J. C.; Vandenbol, Micheline ULg; Vissers, S. et al

in Archives Internationales de Physiologie et de Biochimie (1986), 94

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See detailCloning, biochemical and structural studies of an alcohol dehydrogenase from the Antarctic bacterium Moraxella sp. TAE 123
Tsigos, I.; Georlette, Daphné; Papanikolau, Y. et al

Poster (2002)

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See detailCloning, Nucleotide Sequence and Amplified Expression of the Gene Encoding the Extracellular Metallo (Zn) Dd-Peptidase of Streptomyces Albus G
Duez, Colette ULg; Lakaye, Bernard ULg; Houba, Simone et al

in FEMS Microbiology Letters (1990), 71(1-2), 215-219

The gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G has been cloned in Escherichia coli DH5 alpha MCR via pBR322 or 325, and then transferred into Streptomyces lividans ... [more ▼]

The gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G has been cloned in Escherichia coli DH5 alpha MCR via pBR322 or 325, and then transferred into Streptomyces lividans TK24 via pIJ486, with substantial amplification of the expressed DD-peptidase. The gene has the information for the synthesis of a 255 amino acid precursor, the amino terminal region of which has the characteristic features of a signal peptide. The primary structure as deduced from nucleotide sequencing confirms that previously determined by chemical methods except for the occurrence of an Asp instead of Asn at position 1 and an additional Ala immediately downstream of Pro67. [less ▲]

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See detailCloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10
Arpigny, J. L.; Feller, Georges ULg; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1993), 1171(3), 331-3

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M ... [more ▼]

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold. [less ▲]

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See detailCloning, Sequencing and Characterization of PIT1 Gene in Ovis aries
Bastos, Estella; Santos, Ingrid; Parmentier Isabelle et al

in Genetica (2006), 126

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See detailCloning, sequencing and overexpression in Escherichia coli of the alginate lyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases
Chavagnat, Frederic; Duez, Colette ULg; Guinand, Micheline et al

in Biochemical Journal (1996), 319(Pt 2), 575-583

A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form ... [more ▼]

A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form of a 233-amino-acid precursor. P. alginovora Aly has been overproduced in E. coli with a His-tag sequence fused at the C-terminal end under conditions in which the formation of inclusion bodies is avoided. His-tagged P. alginovora Aly has the same enzymic properties as the wild-type enzyme and has the specificity of a mannuronate lyase. It can be purified in a one-step procedure by affinity chromatography on Ni(2+)-nitriloacetate resin. The yield is of 5 mg of enzyme per litre of culture. The amplification factor is 12.5 compared with the level of production by wild-type P. alginovora. The six alginate lyases of known primary structure fall into three distinct classes, one of which comprises the pair P. alginovora Aly and Klebsiella pneumoniae Aly. [less ▲]

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See detailClONO2 total vertical column abundances above the Jungfraujoch Station, 1986-1994: Long-term trend and winter-spring enhancements
Rinsland, C. P.; Zander, Rodolphe ULg; Demoulin, Philippe ULg et al

in Journal of Geophysical Research (1996), 101(D2), 3891-3899

Total vertical column abundances of chlorine nitrate (ClONO2) have been retrieved from 0.006 cm(-1) resolution solar absorption spectra recorded at the International Scientific Station of the Jungfraujoch ... [more ▼]

Total vertical column abundances of chlorine nitrate (ClONO2) have been retrieved from 0.006 cm(-1) resolution solar absorption spectra recorded at the International Scientific Station of the Jungfraujoch (ISSJ) in the Swiss Alps (altitude 3.58 km, latitude 46.5 degrees N, longitude 8.0 degrees E) on 105 days between June 1986 and November 1994. The analysis is based on spectral fittings of the ClONO2 nu(4) band Q branch at 780.21 cm(-1) and the interferences occurring in the same spectral region. The ISSJ measurements show a regular long-term increase in the ClONO2 column with an occasional factor of 2 to 3 enhancements during the midwinter to early spring. Excluding data from this time of the year, the ISSJ database reflects a linear rate of increase and la uncertainty equal to (4.0 +/- 0.7)% yr(-1) referenced to 1990.0. The corresponding ClONO2 total vertical columns for mid-1986 and mid-1994 are equal to 0.92 and 1.26 x 10(15) molecules cm(-2), respectively. The high ClONO2 columns and high HF/HCl column ratios sometimes measured during winter indicate the occasional presence of chemically processed air above the station. This is corroborated by trajectories calculated for the stratospheric air masses sounded on these occasions. [less ▲]

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See detailClosantel Intoxication in a Dog
Mc Entee, Kathleen ULg; Grauwels, Magda ULg; Clercx, Cécile ULg et al

in Veterinary and Human Toxicology (1995), 37(3), 234-6

A case of overdosage with closantel, a salicynalide derivative, in a dog is described. The dog received 6 times the recommended dosage. Closantel induced optic neuritis, retinal degeneration, partial ... [more ▼]

A case of overdosage with closantel, a salicynalide derivative, in a dog is described. The dog received 6 times the recommended dosage. Closantel induced optic neuritis, retinal degeneration, partial deafness, hepatotoxicosis and myopathy. Only the blindness was irreversible. The therapy included albumin administration to reduce the acute toxicity of closantel. [less ▲]

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See detailA close look at the RGS spectra of the O4Ief star Zeta Pup
Rauw, Grégor ULg; Flores, A.; Nazé, Yaël ULg et al

Poster (2010)

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See detailClose projected QSO-Galaxy associations: are they real?
Claeskens, Jean-François ULg; Surdej, Jean ULg

in Astronomy and Astrophysics (1998), 335

The present status of close projected associations between high redshift quasars and foreground galaxies is reviewed in the framework of gravitational lensing, adopting both an observational and a ... [more ▼]

The present status of close projected associations between high redshift quasars and foreground galaxies is reviewed in the framework of gravitational lensing, adopting both an observational and a theoretical approach. The Non Singular Isothermal Spherical lens model (Hinshaw & Krauss 1987) is used. We confirm that a stronger overdensity q of bright galaxies is expected at small projected angular separations (<= 3arcsec ) from high redshift and bright (thus highly luminous) quasars (HLQs). The effects of a non singular galactic core radius and/or microlensing only slightly enhance this expected overdensity. The predictions reproduce pretty well the scarce observations which are presently available, with the exception of two rather high overdensities previously claimed in the literature (e.g. q=2.9 for theta <= 6arcsec , Webster & Hewett 1990). Uncontrolled morphological selections may affect these controversial results so that additional unbiased observations are badly needed before rejecting gravitational lensing as the mechanism to produce the observed close angular QSO-galaxy associations. We describe three new selected and bias-free samples consisting of a total of 219 different HLQs. Although an apparent overdensity is detected in two of them, these galaxy excesses are found to be statistically not significant. Firm confirmation of the reality of close projected QSO-Galaxy associations is hampered by small number statistics: the expected and observed absolute numbers of such QSO-galaxy associations are still presently found to be very small. Therefore, the corresponding error bars are quite large. We conclude that the best observational strategy to significantly detect (at 3sigma ) an overdensity of galaxies near HLQs is to observe about 1500 HLQs (M_V ~ -29) down to a limiting magnitude Rlim ~ 23. Future automated surveys may provide us with such a large and unbiased database. In conclusions, we find that the lensing-induced correlation between galaxies and HLQs at small angular separations consists of a very interesting but weak effect, much less sensitive to cosmological or astrophysical parameters than the number of multiply imaged sources expected within a sample of HLQs. Based on data collected at the European Southern Observatory, La Silla, Chile [less ▲]

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See detailClose-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors
Tourillon, Gérard; Dreesen, Laurent ULg; Volcke, Cédric et al

in Journal of Materials Science (2009), 44(24), 6805-6810

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy ... [more ▼]

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy performed in the Total Internal Reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. [less ▲]

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See detailClose-up of primary and secondary asteroseismic CoRoT targets and the ground-based follow-up observations
Uytterhoeven, K.; Poretti, E.; Rainer, M. et al

in Journal of Physics Conference Series (2008), 118

To optimise the science results of the asteroseismic part of the CoRoT satellite mission a complementary simultaneous ground-based observational campaign is organised for selected CoRoT targets. The ... [more ▼]

To optimise the science results of the asteroseismic part of the CoRoT satellite mission a complementary simultaneous ground-based observational campaign is organised for selected CoRoT targets. The observations include both high-resolution spectroscopic and multicolour photometric data. We present the preliminary results of the analysis of the ground-based observations of three targets. A line-profile analysis of 216 high-resolution FEROS spectra of the δ Sct star HD 50844 reveals more than ten pulsation frequencies in the frequency range 5-18 d[SUP]-1[/SUP], including possibly one radial fundamental mode (6.92 d[SUP]-1[/SUP]). Based on more than 600 multi-colour photometric datapoints of the β Cep star HD 180642, spanning about three years and obtained with different telescopes and different instruments, we confirm the presence of a dominant radial mode ν[SUB]1[/SUB] = 5.48695 d[SUP]-1[/SUP], and detect also its first two harmonics. We find evidence for a second mode ν[SUB]2[/SUB] = 0.3017 d[SUP]-1[/SUP], possibly a g-mode, and indications for two more frequencies in the 7-8 d[SUP]-1[/SUP] domain. From Stromgren photometry we find evidence for the hybrid 5 Sct/γ Dor character of the F0 star HD 44195, as frequencies near 3 d[SUP]-1[/SUP] and 21 d[SUP]-1[/SUP] are detected simultaneously in the different filters. [less ▲]

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See detailA closed form technique to predict springback in creep age-forming
Jeunechamps, Pierre-Paul ULg; Ho, K. C.; Lin, J. et al

in International Journal of Mechanical Sciences (2006), 48(6), 621-629

An efficient numerical method has been developed and programmed to predict springback in creep age-forming (CAF) of alummium shell components. Physically based unified creep-ageing constitutive equations ... [more ▼]

An efficient numerical method has been developed and programmed to predict springback in creep age-forming (CAF) of alummium shell components. Physically based unified creep-ageing constitutive equations determined for AA7010 at 150 degrees C are used to evaluate age-hardening and creep deformation in CAF processes. The studies are mainly concentrated on the pure bending of aluminium plates. Effects of plate thickness, single and double curvature bending, ageing time and stress relaxation features on springback are investigated. It is shown that the proposed algorithm can predict springback accurately and gives a relationship between geometrical data and springback. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailClosed-form dual perturb and combine for tree-based models
Geurts, Pierre ULg; Wehenkel, Louis ULg

in Proceedings of the International Conference on Machine Learning (ICML 2005) (2005)

This paper studies the aggregation of predictions made by tree-based models for several perturbed versions of the attribute vector of a test case. A closed-form approximation of this scheme combined with ... [more ▼]

This paper studies the aggregation of predictions made by tree-based models for several perturbed versions of the attribute vector of a test case. A closed-form approximation of this scheme combined with cross-validation to tune the level of perturbation is proposed. This yields soft-tree models in a parameter free way, and reserves their interpretability. Empirical evaluations, on classification and regression problems, show that accuracy and bias/variance tradeoff are improved significantly at the price of an acceptable computational overhead. The method is further compared and combined with tree bagging. [less ▲]

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See detailClosed-Loop Learning of Visual Control Policies
JODOGNE, Sébastien ULg

Doctoral thesis (2006)

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