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See detailCharacterization of fine aggregates in concrete by different experimental approaches
He, Huan; Courard, Luc ULg; Pirard, Eric ULg et al

in Proceedings 13 Int. Congress for Stereology (2011, October)

Being its major component, aggregate can occupy up to three-quarter of the volume of concrete. The structure of aggregate formed in hardened state impacts largely on mechanical and durability properties ... [more ▼]

Being its major component, aggregate can occupy up to three-quarter of the volume of concrete. The structure of aggregate formed in hardened state impacts largely on mechanical and durability properties of concrete. On another hand, physical characteristics of aggregate are primarily assumed to be relevant to granular behavior of aggregate. Therefore, characterization of aggregate is of high relevance to concrete studies. In this study, different types of fine aggregate used in concrete, namely river sand and crushed limestone, are selected for morphological characterization. Traditional sieve analysis and laser diffraction method are employed for separation and size analysis of specimens. Different types of fine aggregate samples with comparable size ranges are then analyzed by two advanced dry (static) and wet (dynamic) image analysers. These new analysers are especially suitable for characterization of fine particles, which is difficult by traditional image analysis equipments. Size and shape characteristics of different fine aggregates will be revealed by different experimental methods. The results on different parameters for shape characterization will be compared and discussed [less ▲]

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See detailCharacterization Of Functional Residues In The Interfacial Recognition Domain Of Lecithin Cholesterol Acyltransferase (Lcat)
Peelman, F.; Vanloo, B.; Perez-Mendez, O. et al

in Protein Engineering (1999), 12(1), 71-8

Lecithin cholesterol acyltransferase (LCAT) is an interfacial enzyme active on both high-density (HDL) and low-density lipoproteins (LDL). Threading alignments of LCAT with lipases suggest that residues ... [more ▼]

Lecithin cholesterol acyltransferase (LCAT) is an interfacial enzyme active on both high-density (HDL) and low-density lipoproteins (LDL). Threading alignments of LCAT with lipases suggest that residues 50-74 form an interfacial recognition site and this hypothesis was tested by site-directed mutagenesis. The (delta56-68) deletion mutant had no activity on any substrate. Substitution of W61 with F, Y, L or G suggested that an aromatic residue is required for full enzymatic activity. The activity of the W61F and W61Y mutants was retained on HDL but decreased on LDL, possibly owing to impaired accessibility to the LDL lipid substrate. The decreased activity of the single R52A and K53A mutants on HDL and LDL and the severer effect of the double mutation suggested that these conserved residues contribute to the folding of the LCAT lid. The membrane-destabilizing properties of the LCAT 56-68 helical segment were demonstrated using the corresponding synthetic peptide. An M65N-N66M substitution decreased both the fusogenic properties of the peptide and the activity of the mutant enzyme on all substrates. These results suggest that the putative interfacial recognition domain of LCAT plays an important role in regulating the interaction of the enzyme with its organized lipoprotein substrates. [less ▲]

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See detailCharacterization of gas transport in low-permeability media: two-phase flow analysis of an in-situ experiment
Gerard, Pierre; Radu, Jean-Pol ULg; de la Vaissière, Rémi et al

in POROMECHANICS V: Proceedings of the Fifth Biot Conference on Poromechanics (2013)

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See detailCharacterization of gels via solvent desorption measurements
Gommes, Cédric ULg; Noville, Francis ULg; Pirard, Jean-Paul ULg

in Adsorption (2007), 13(5-6), 533-540

The present paper shows how a standard volumetric adsorption device can be used to measure solvent desorption isotherms on gels. As gels are very soft materials, they shrink significantly during the ... [more ▼]

The present paper shows how a standard volumetric adsorption device can be used to measure solvent desorption isotherms on gels. As gels are very soft materials, they shrink significantly during the measurement, and the data have to be analyzed in terms of the mechanical properties of the gel's skeleton. Methanol desorption isotherms are measured on a series of silica gels, and the results are compared with independent characterizations, notably beam bending. [less ▲]

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See detailCharacterization of gels via solvent desorption measurements
Gommes, Cédric ULg; Noville, Francis; Pirard, Jean-Paul ULg

Poster (2007, May)

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See detailCharacterization of growth hormone-binding protein in cattle plasma: prolactin-binding activity and 24-hour profile.
Massart, S.; Ban, A. M.; Renaville, Robert ULg et al

in Domestic Animal Endocrinology (1996), 13(1), 47-57

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these ... [more ▼]

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these molecules was investigated. The preincubation of bull plasma with iodinated bovine growth hormone (bGH) or bovine prolactin (bPRL), followed by gel filtration chromatography (Superdex 200; 1.6 x 60 cm column), resulted in the formation of essentially two complexes. The majority of [125I]bPRL eluted with the first one (M(r) approximately 600 kDa), whereas [125I]bGH mainly appeared in the second one (M(r) approximately 70 kDa). The fractions corresponding to these two peaks were analyzed by western ligand blotting (WLB), under reducing conditions. WLB revealed, respectively, 190-, 56-, 52-, and 28-kDa bands for the first peak and only 52- and 28-kDa bands for the second one. The nature of the 600-kDa peak is at present undetermined, but the 70-kDa one was previously identified as high-affinity GHBP. Displacement studies demonstrated that bGH and bPRL were both able to bind to this GHBP, because the bGH- and bPRL-binding activities of this protein could be saturated by an excess of either of these two hormones. This was indirectly confirmed by the close correlation (r = 0.615; P = 0.0001; n = 155) observed between plasma bGH- and bPRL-binding activities, because this correlation could suggest that both ligands are bound to the same proteins. The temporal concentrations of plasma GHBP were measured in samples collected at 20-min intervals for 24 hr from 8 young bulls. The evaluation of GHBP was realized by WLB, followed by densitometric analysis. Some fluctuations were observed, but these were not correlated with bGH release, even with a +/- 2-hr lag period. In summary, we found that bovine high-affinity GHBP binds not only bGH, but also bPRL. A second type of protein, of higher molecular weight, also binds these two hormones, but further investigations are needed to determine its nature. Finally, GHBP concentrations in cattle blood plasma apparently show fluctuations over a 24-hr period, but no correlation was found between these fluctuations and plasma growth hormone concentrations. [less ▲]

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See detailCharacterization of H3+xPMo12 xVxO40 heteropolyacids supported on HMS mesoporous molecular sieve and their catalytic performance in propene oxidation
Benadji, Siham; Eloy, Pierre; Léonard, Alexandre ULg et al

in Microporous and Mesoporous Materials (2012), 154

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See detailCharacterization of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) overwintering sites
Fassotte, Bérénice ULg; Durieux, Delphine ULg; Haubruge, Eric ULg et al

Poster (2012, February 10)

The invasive multicoloured Asian ladybird, Harmonia axyridis (Pallas), forms large aggregations inside dwellings to overwinter. In order to highlight the specific features of infested houses, we ... [more ▼]

The invasive multicoloured Asian ladybird, Harmonia axyridis (Pallas), forms large aggregations inside dwellings to overwinter. In order to highlight the specific features of infested houses, we investigated a large number of overwintering sites in Wallonia between 2007 and 2011. These sites were characterized through a survey sent to homeowners confronted to invasion problems. The results indicate that H. axyridis preferentially selects isolated brick houses with red or white fronts to take shelter. Aggregations are mostly located at the first floor, essentially inside south or west oriented rooms. Furthermore, ladybirds generally gathered into wooden windows frames facing south or west, and to a lesser extent, in the upper corners of walls presenting the same orientation. [less ▲]

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See detailCharacterization of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) overwintering sites
Fassotte, Bérénice ULg; Durieux, Delphine ULg; Francis, Frédéric ULg et al

Poster (2012, August)

Originally introduced as a biological control agent, the multicoloured Asian ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), has become an invasive pest throughout Europe and North ... [more ▼]

Originally introduced as a biological control agent, the multicoloured Asian ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), has become an invasive pest throughout Europe and North America in the last few years. Although its effectiveness to control aphid and coccid populations was impressive, some negative impacts appeared rapidly, notably on human health. Indeed, to protect themselves from cold temperatures, H. axyridis individuals move inside dwellings and buildings and form large aggregations in concealed portions of structures to overwinter. The aggregating beetles are responsible for some annoyances due to, on one hand, the number of individuals inside homes and, on the other hand, the hemolymph secretions they release when they are disturbed, which can cause allergic reactions. In order to highlight the specific features of infested houses, we investigated a large number of overwintering sites in Wallonia between 2007 and 2011. These sites were characterized through a survey sent to homeowners confronted to invasion problems. This survey was mainly focused on a general description of the infested house (type, colour, infested floor(s), building material), the orientation of the colonized rooms and the position of the beetles’ cluster. The collected data indicate that H. axyridis preferentially selects isolated brick houses with red or white fronts to take shelter. Aggregations are mostly located at the first floor, essentially inside south, west or southwest oriented rooms. Furthermore, ladybeetles generally gathered into wooden windows frames facing south, west or southwest and to a lesser extent, in the upper corners of walls presenting the same orientation. All these results contribute to improve the knowledge on the aggregative behaviour of H. axyridis and could promote the development of more specific and efficient management methods to prevent massive infestations into dwellings, such as artificial shelters or trapping systems located at the outside of buildings. [less ▲]

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See detailCharacterization of hemicellulosic fractions from spelt hull extracted by different methods
Escarnot, Emmanuelle; Aguedo, Mario ULg; Paquot, Michel ULg

in Carbohydrate Polymers (2011), 85

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See detailCharacterization of hepatitis C virus-induced nasal mucosa remodelling.
El Shazly, Amr ULg; Arafa, Mohammad; Roncarati, Patrick ULg et al

in Histopathology (2010), 57(3), 488-92

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See detailCharacterization of hyperporous polyurethane-based gels by non-intrusive mercury porosimetry
Pirard, René ULg; Rigacci, Arnaud; Marechal, Jacqueline ULg et al

in Polymer (2003), 44(17), 4881-4887

Evaporative drying of polyurethane-based gels produces xerogels. Supercritical drying after replacement of interstitial liquid by supercritical CO2 produces aerogels. SEM micrographs show that both ... [more ▼]

Evaporative drying of polyurethane-based gels produces xerogels. Supercritical drying after replacement of interstitial liquid by supercritical CO2 produces aerogels. SEM micrographs show that both materials are made up of small size particles gathered up in filament-shaped, strongly cross-linked aggregates. Density measurements show that they both have a large pore volume. When submitted to mercury porosimetry, the behavior of these materials is similar to that of inorganic aerogels, as previously observed. Mercury does not penetrate the pore network, but the whole material is densified. The usual Washburn equation cannot be used to analyze the mercury porosimetry. A well-suited equation based on a buckling model of filament-shaped aggregates has been developed in order to determine the pore volume distribution of mineral dried gels. This equation is also valid for analyzing the texture of organic hyperporous materials like polyurethane dried nanoporous gel. (C) 2003 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailCharacterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple PCR sequencing assays
Del Médico Zajac, M. P.; Romera, S. A.; Ladelfa, M. F. et al

in Journal of Virological Methods (2009)

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See detailCharacterization of intestinal cnf1+ Escherichia coli from weaned pigs
Toth, I.; Oswald, E.; Mainil, Jacques ULg et al

in International Journal of Medical Microbiology (2000), 290

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See detailCharacterization of inulin stored at different relative humidities by MDSC and X-ray diffraction, related to soption isotherms.
Ronkart, Sébastien; Fougnies, C.; Paquot, Michel ULg et al

Poster (2004, December)

Detailed reference viewed: 32 (15 ULg)