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See detailCharacteristics of Saturn's FUV aurora observed with the Space Telescope Imaging Spectrograph
Gérard, Jean-Claude ULg; Grodent, Denis ULg; Gustin, Jacques ULg et al

in Journal of Geophysical Research. Space Physics (2004), 109

We analyze a set of 15 FUV images obtained between October 1997 and January 2001 with the Hubble Space Telescope Imaging Spectrograph (STIS), providing a good view of Saturn's south auroral oval. It is ... [more ▼]

We analyze a set of 15 FUV images obtained between October 1997 and January 2001 with the Hubble Space Telescope Imaging Spectrograph (STIS), providing a good view of Saturn's south auroral oval. It is found that the morphology and brightness distribution of the aurora are dynamical with variations occurring on time scales of hours or less. The dayside main oval lies between 70° and 80° and is generally brighter and thinner in the morning than in the afternoon sector. The afternoon sector is characterized by more diffuse emission at higher latitudes. Weak emission is also observed poleward of the main oval up to the pole. A spot of enhanced auroral precipitation, tentatively identified as the optical signature of the dayside cusp, is sometimes observed poleward of the main oval in the noon sector, especially during periods when the morning arc is not fully developed. A spiral structure of the main oval with arcs at two latitudes in the same sector is occasionally observed. The brightness of the main oval ranges from below the STIS threshold of 1 kR of H[SUB]2[/SUB] emission up to ~75 kR. The total electron precipitated power varies between 20 and 140 GW, that is, comparable to the Earth's active aurora but about two orders of magnitude less than on Jupiter. An increasing trend of the precipitated power between the 1997 and the 2000-2001 observations may be related to the rising solar activity. Six spectra of the aurora in the noon sector covering the 1200-1700 Å range are dominated by emissions of the Lyman-alpha line and H[SUB]2[/SUB] Werner and Lyman bands. Their comparison with a synthetic model of electron excited H[SUB]2[/SUB] emissions indicates the presence of a weak absorption below 1400 Å by a column of methane ranging between 7 × 10[SUP]15[/SUP] and 2 × 10[SUP]16[/SUP] cm[SUP]-2[/SUP]. The corresponding energy of the primary auroral electrons is estimated 12 +/- 3 keV, using a low-latitude model atmosphere based on Voyager occultation measurements. The main oval brightness and the characteristic electron energy are generally consistent with recent models of Saturn's aurora, which colocate the main oval with the narrow upward field-aligned current system associated with departure from plasma corotation near the open-closed field line boundary. The latitude of the bright morning arc is somewhat lower than model predictions based on the plasma flow velocity measured by Voyager in the middle magnetosphere. [less ▲]

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See detailCharacteristics of Saturn's polar atmosphere and auroral electrons derived from HST/STIS, FUSE and Cassini/UVIS spectra
Gustin, Jacques ULg; Gérard, Jean-Claude ULg; Pryor, Wayne et al

in Icarus: International Journal of Solar System Studies (2009), 200

Ultraviolet (UV) spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic ... [more ▼]

Ultraviolet (UV) spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic Explorer (FUSE) have been analyzed. Comparisons between the observed spectra and synthetic models of electron-excited H[SUB]2[/SUB] have been used to determine various auroral characteristics. Far ultraviolet (FUV: 1200 1700 Å) STIS and UVIS spectra exhibit, below 1400 Å, weak absorption due to methane, with a vertical column ranging between 1.4×10[SUP][/SUP] and 1.2×10[SUP][/SUP]cm[SUP][/SUP]. Using the low-latitude Moses et al. [Moses, J.I., Bézard, B., Lellouch, E., Feuchtgruber, H., Gladstone, G.R., Allen, M., 2000. Icarus, 143, 244 298] atmospheric model of Saturn and an electron energy H[SUB]2[/SUB] column relationship, these methane columns are converted into the mean energy of the primary precipitating electrons, estimated to lie in the range 10 18 keV. This result is confirmed by the study of self-absorption with UVIS and FUSE extreme ultraviolet (EUV: 900 1200 Å) spectra. Below 1200 Å, it is seen that transitions connecting to the v[SUP][/SUP]<2 vibrational levels of the H[SUB]2[/SUB] electronic ground state are partially self-absorbed by H[SUB]2[/SUB] molecules overlying the auroral emission. Because of its low spectral resolution (Ë 5.5 Å), the UVIS EUV spectrum we analyzed does not allow us to unequivocally determine reasonable ranges of temperatures and H[SUB]2[/SUB] columns. On the other hand, the high spectral resolution (Ë 0.2 Å) of the FUSE LiF1a and LiF2a EUV spectra we examined resolve the H[SUB]2[/SUB] rotational lines and makes it possible to determine the H[SUB]2[/SUB] temperature. The modeled spectrum best fitting the FUSE LiF1a observation reveals a temperature of 500 K and self-absorption by a H[SUB]2[/SUB] vertical column of 3×10[SUP][/SUP]cm[SUP][/SUP]. When converted to energy of precipitating electrons, this H[SUB]2[/SUB] column corresponds to primary electrons of Ë 10 keV. The model that best fits the LiF2a spectrum is characterized by a temperature of 400 K and is not self-absorbed, making this segment ideal to determine the H[SUB]2[/SUB] temperature at the altitude of the auroral emission. The latter value is in agreement with temperatures obtained from H3+ infrared polar spectra. Self-absorption is detectable in the LiF2a segment for H[SUB]2[/SUB] columns exceeding 6×10[SUP][/SUP]cm[SUP][/SUP], which sets the maximum mean energy determined from the FUSE observations to Ë 15 keV. The total electron energy range of 10 18 keV deduced from FUV and EUV observations places the auroral emission peak between the 0.1 and 0.3 mubar pressure levels. These values should be seen as an upper limit, since most of the Voyager UVS spectra of Saturn's aurora examined by Sandel et al. [Sandel, B.R., Shemansky, D.E., Broadfoot, A.L., Holberg, J.B., Smith, G.R., 1982. Science 215, 548] do not exhibit methane absorption. The auroral H[SUB]2[/SUB] emission is thus likely located above but close to the methane homopause. The H[SUB]2[/SUB] auroral brightness in the 800 1700 Å bandwidth varies from 2.9 kR to 139 kR, comparable to values derived from FUV Faint Object Camera (FOC) and STIS images. [less ▲]

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See detailCharacteristics of Saturn's polar atmosphere and auroral electrons derived from HST/STIS, FUSE and Cassini/UVIS spectra
Gustin, Jacques ULg; Pryor, W.; Feldman, P. et al

Poster (2008, December 01)

Ultraviolet spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic ... [more ▼]

Ultraviolet spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic Explorer (FUSE) have been compared to synthetic spectra of electron-excited H2 in order to derive various auroral characteristics, such as the energy of the primary precipitating electrons and the H2 temperature at the altitude of the aurora. Two physical processes have been exploited: the absorption by hydrocarbons in the FUV and H2 self-absorption in the EUV. We find energies in the range 10-18 keV, which locates Saturns's aurora between 0.1 and 0.3 μ bar. We also determined that the auroral H2 emission is characterized by a temperature of ~400K, consistent with temperatures measured in the infrared, but much higher than what is expected from equatorial atmospheric models. These new results bring valuable constraints on both polar atmospheric models and theoretical studies of the ionosphere-magnetosphere coupling. [less ▲]

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See detailCharacteristics of Saturn's polar atmosphere and auroral electrons derived from HST/STIS, FUSE and Cassini/UVIS spectra
Gustin, Jacques ULg; Pryor, W.; Feldman, P. et al

Poster (2008, December 01)

Ultraviolet spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic ... [more ▼]

Ultraviolet spectra of Saturn's aurora obtained with the Hubble Space Telescope Imaging Spectrograph (STIS), the Cassini Ultraviolet Imaging Spectrograph (UVIS) and the Far Ultraviolet Spectroscopic Explorer (FUSE) have been compared to synthetic spectra of electron-excited H2 in order to derive various auroral characteristics, such as the energy of the primary precipitating electrons and the H2 temperature at the altitude of the aurora. Two physical processes have been exploited: the absorption by hydrocarbons in the FUV and H2 self-absorption in the EUV. We find energies in the range 10-18 keV, which locates Saturns's aurora between 0.1 and 0.3 μ bar. We also determined that the auroral H2 emission is characterized by a temperature of ~400K, consistent with temperatures measured in the infrared, but much higher than what is expected from equatorial atmospheric models. These new results bring valuable constraints on both polar atmospheric models and theoretical studies of the ionosphere-magnetosphere coupling. [less ▲]

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See detailCharacteristics of the iron moment in Dy-Fe and Dy-FeCo amorphous alloys studied by X-ray magnetic circular dichroism
Fleury-Frenette, Karl ULg; Dhesi, S. S.; van der Laan, G. et al

in Journal of Magnetism & Magnetic Materials (2000), 220(1), 45-51

The local magnetic moment of Fe in Dy-Fe and Dy-FeCo amorphous alloys has been studied using X-ray absorption spectroscopy and X-ray magnetic circular dichroism (XMCD). The Fe orbital and spin magnetic ... [more ▼]

The local magnetic moment of Fe in Dy-Fe and Dy-FeCo amorphous alloys has been studied using X-ray absorption spectroscopy and X-ray magnetic circular dichroism (XMCD). The Fe orbital and spin magnetic moments have been obtained for a range of alloy compositions by applying the sum rules to the XMCD spectra. The room temperature variations of the average components of the Fe moments as a function of Dy concentration and with the substitution of Fe by Co have been determined. A sharp reversal of the total magnetic moment was found at 28 +/- 1 at% Dy for both alloys. (C) 2000 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailThe characteristics of the O2 Herzberg II and Chamberlain bands observed with VIRTIS/Venus Express
Migliorini, A.; Piccioni, G.; Gérard, Jean-Claude ULg et al

in Icarus (2013)

The oxygen Venus nightglow emissions in the visible spectral range have been known since the early observations from the Venera spacecraft. Recent observations with the VIRTIS instrument on board Venus ... [more ▼]

The oxygen Venus nightglow emissions in the visible spectral range have been known since the early observations from the Venera spacecraft. Recent observations with the VIRTIS instrument on board Venus Express allowed us to re-examine the Herzberg II system of O2 and to further study its vertical distribution, in particular the (0–m00 with m00 = 7–13) bands. The present work describes the vertical profile of the observed bands and relative intensities from limb observation data. The wavelength-integrated intensities of the Herzberg II bands, with m00 = 7–11, are inferred from the recorded spectra. The resulting values lie in the range of 84–116 kR at the altitudes of maximum intensity, which are found to lie in the range of 93–98 km. Three bands of the Chamberlain system, centered at 560 nm, 605 nm, and 657 nm have been identified as well. Their emission peak is located at about 100 km, 4 km higher than the Herzberg II bands. For the first time, the O2 nightglow emissions were investigated simultaneously in the visible and in the IR spectral range, showing a good agreement between the peak position for the Herzberg II and the O2ða1Dg—X3R g Þ bands. An airglow model, proposed by Gérard et al. (Gérard, J.C., Soret, L., Migliorini, A., Piccioni, G. [2012]. Icarus.) starting from realistic O and CO2 vertical distributions derived from Venus-Express observations, allows reproduction of the observed profiles for the three O2 systems. [less ▲]

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See detailCharacteristics of the pulsatile liberation of LH in Italian Simmental cows from the dry period to early lactation
Prandi, Alberto; Motta, Mario; Rossi, A. et al

in Bulletin des Recherches Agronomiques de Gembloux (1995), 30

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See detailCharacteristics of the size distribution of recent and historical landslides in a populated hilly region
Van den Eeckhaut, M.; Poesen, J.; Govers, G. et al

in Earth and Planetary Science Letters (2007), 256(3-4), 588-603

Despite the availability of studies on the frequency density of landslide areas in mountainous regions, frequency-area distributions of historical landslide inventories in populated hilly regions are ... [more ▼]

Despite the availability of studies on the frequency density of landslide areas in mountainous regions, frequency-area distributions of historical landslide inventories in populated hilly regions are absent. This study revealed that the frequency-area distribution derived from a detailed landslide inventory of the Flemish Ardennes (Belgium) is significantly different from distributions usually obtained in mountainous areas where landslides are triggered by large-scale natural causal factors such as rainfall, earthquakes or rapid snowmelt. Instead, the landslide inventory consists of the superposition of two populations, i.e. (i) small (<1-2 . 10(-2) km(2)), shallow complex earth slides that are at most 30 yr old, and (ii) large (> 1-2 . 10(-2) km(2)), deep-seated landslides that are older than 100 yr. Both subpopulations are best represented by a negative power-law relation with exponents of -0.58 and -2.31 respectively. This study focused on the negative power-law relation obtained for recent, small landslides, and contributes to the understanding of frequency distributions of landslide areas by presenting a conceptual model explaining this negative power-law relation for small landslides in populated hilly regions. According to the model hilly regions can be relatively stable under the present-day environmental conditions, and landslides are mainly triggered by human activities that have only a local impact on slope stability. Therefore, landslides caused by anthropogenic triggers are limited in size, and the number of landslides decreases with landslide area. The frequency density of landslide areas for old landslides is similar to those obtained for historical inventories compiled in mountainous areas, as apart from the negative power-law relation with exponent -2.31 for large landslides, a positive power-law relation followed by a rollover is observed for smaller landslides. However, when analysing the old landslides together with the more recent ones, the present-day higher temporal frequency of small landslides compared to large landslides, obscures the positive power-law relation and rollover. (C) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailCharacteristics of Urban Food Insecurity: The Case of Kinshasa
Lebailly, Philippe ULg; Muteba Kalala, Damien ULg

in African Review of Economics and Finance (2011), 3(1), 58-68

Feeding Kinshasa? How to ensure food security for millions of inhabitants who have to survive on low wages well below the poverty line? This is the daily challenge for a multitude of poor people in ... [more ▼]

Feeding Kinshasa? How to ensure food security for millions of inhabitants who have to survive on low wages well below the poverty line? This is the daily challenge for a multitude of poor people in Kinshasa! Some see this situation as an opportunity for strengthening local producers. In the Democratic Republic of Congo (DRC), the constraints affecting the agricultural economy mean it cannot supply the city of Kinshasa on the basis of predominantly local production but through food imports. In a context of crisis as experienced in the DRC, food is of strategic importance and the focus is essentially on quantity: households turn to cheaper products and high calorie intake at the expense of high-protein foods, leading to unbalanced diets. This imbalance is exacerbated by an influx of imported products, often of poor nutritional quality but at very competitive prices and responding to new eating habits of urban consumers. To understand the issue of food security in DRC, a country with high agricultural potential but 70% of whose population is affected by food insecurity, various research devices have been established by an interdisciplinary research group to support policy, funded by the Belgian Development Cooperation Department (DGDC / CUD): the Research Group Supporting the Policy for Food and Agriculture in Africa (GRAP 3A). This paper presents one of the main focuses of the inter-university research program conducted jointly with researchers from the University of Kinshasa: an approach to food insecurity and strategies for a sustainable food policy based on the study of household food consumption in Kinshasa carried out by various surveys. [less ▲]

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See detailCharacterization of Clostridium perfringens isolates from goats.
Phukan, A.; Dutta, G.-N.; Daube, Georges ULg et al

in Indian Veterinary Journal (The) (1997), 74

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See detailCharacterization and Adsorption Study of Thymol on Pillared Bentonite
El Miz, Mohamed; Salhi, S.; Chraibi, I. et al

in Open Journal of Physical Chemistry (2014), 4(3), 98-116

Pillared clay (PILC) was prepared from Moroccan clay and characterized, and its aqueous thymol adsorption capacities were studied using a batch equilibrium technique. So, we tested the encapsulation of ... [more ▼]

Pillared clay (PILC) was prepared from Moroccan clay and characterized, and its aqueous thymol adsorption capacities were studied using a batch equilibrium technique. So, we tested the encapsulation of thymol by aluminum pillared clay (PILC). The PILCs displayed a total surface area of 270 m2/g, a total pore volume of 0.246 cm3/g and an average pore diameter of 8.9 Å, which corresponds to the size of Al13 forming the pillars between the clay layers. The adsorption capacity shown by the PILCs for thymol from water is close to 319 mg∙g−1 for low solid/liquid ratio (0.2%). This result suggests that the PILCs have both hydrophobic and hydrophilic characteristics, as a result of the presence of silanol and siloxane groups formed during the pillaring and calcination of the PILCs. The experimental data were analyzed by the Freundlich and the Langmuir isotherm types for low values of equilibrium concentration. The rise of the isotherm in this range of concentrations was related to the affinity of thymol for clay sites, and the equilibrium data fitted well with the Freundlich model with maximum adsorption capacity of 319.51 mg/g for a ratio RS/L = 0.2%. Pseudo-first and pseudo-second-order kinetic models were tested with the experimental data and pseudo-first order kinetics was the best for the adsorption of thymol with coefficients of correlation R² ≥0.986, and the adsorption was rapid with 90% of the thymol adsorbed within the first 20 min. [less ▲]

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See detailCharacterization and Cloning of Chitin Deacetylases from Rhizopus Circinans
Gauthier, Carole ULg; Clerisse, Fabienne ULg; Dommes, Jacques ULg et al

in Protein Expression & Purification (2008), 59

Chitin deacetylase catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls. Here a chitin deacetylase secreted by Rhizopus circinans was purified to homogeneity ... [more ▼]

Chitin deacetylase catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls. Here a chitin deacetylase secreted by Rhizopus circinans was purified to homogeneity and partially characterized. The enzyme exhibits an apparent molecular weight of approximately 75kDa. At 37 degrees C it shows optimal activity at pH 5.5-6. Its pH stability and thermal stability are good. Mn(2+) and Mg(2+) slightly enhance the activity of the enzyme and Cu(2+) strongly inhibits it. An R. circinans cDNA library was constructed and screened with a homologous probe synthesized by RT-PCR or with synthetic primers derived from the N-terminal amino-acid sequence of the native purified chitin deacetylase. Three chitin deacetylase cDNAs (RC, D2, and I3/2) were isolated from the cDNA library and sequenced. These cDNAs exhibit features characteristic of chitin deacetylase sequences: the presence of a polysaccharide deacetylase domain, a metal-binding triad, the conserved catalytic residues, and high homology with various chitin deacetylase genes. The cDNAs were cloned in a Pichia pastoris expression system and produced as polyhistidine-tagged proteins. Only one recombinant enzyme (called RC) was active under the tested conditions. It was purified to homogeneity in a single step and further characterized. The protein showed an apparent molecular mass of approximately 75kDa and, like the native enzyme, showed optimal activity at pH 5.5-6 at 37 degrees C. It was strongly inhibited by Cu(2+). The isolation of several chitin deacetylase cDNAs from the same microorganism is discussed. [less ▲]

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See detailCharacterization and dynamics of the auroral electron precipitation during substorms deduced from IMAGE-FUV
Meurant, M.; Gérard, Jean-Claude ULg; Hubert, Benoît ULg et al

in Journal of Geophysical Research. Space Physics (2003), 108(A6),

[1] The FUV imager on board the IMAGE satellite provides simultaneous images of the north polar aurora every 2 min in three spectral channels. The Wideband Imaging Camera (WIC) responds primarily to the N ... [more ▼]

[1] The FUV imager on board the IMAGE satellite provides simultaneous images of the north polar aurora every 2 min in three spectral channels. The Wideband Imaging Camera (WIC) responds primarily to the N-2 LBH bands while one of the Spectral Imagers (SI13) includes the OI 135.6 nm emission and nearby LBH bands. The third channel (SI12) is sensitive to Doppler-shifted Lyman-alpha emission at 121.8 nm generated by proton precipitation. The relative magnitude of the WIC and SI13 signals depends on the altitude distribution of the energy deposition, in response to the differential O-2 absorption and the altitude dependence of the neutral composition. The ratio of simultaneous images from WIC and SI13 is used to derive the spatial distribution of the characteristic energy of the precipitating auroral electrons and the energy flux they carry. The method is described and the uncertainties introduced by possible perturbations of the neutral composition known to occur in the auroral thermosphere are discussed. The first part of this study describes a validation of this method performed by comparing precipitation characteristics derived from FUV with in situ measurements from two coincident passes of the NOAA-16 satellite. They are shown to agree within about 45%. The second part applies this ratio method to analyze the time evolution of auroral activity which occured during two substorms on 28 October 2000. The time evolution is displayed in the form of magnetic local time and magnetic latitude keograms. It is shown that the pattern of the electron average energy distribution exhibits both spatial and temporal changes. Comparison with FAST in situ electron spectrograms confirms the ability of IMAGE to detect precipitation events with a similar to200 km spatial scale. However the characteristics of the physical process leading to electron acceleration cannot be identified with FUV. The highest values of the average energy are colocated in time and space with the largest electron precipitation fluxes. A dawnward motion of bright features is observed in the postmidnight at speeds on the order of 5 magnetic local time hours/UT hour. [less ▲]

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See detailCharacterization and impact of reduced copper plating overburden on 45 nm interconnect performances
Dubreuil, Olivier ULg; Caubet-Hilloutou, Véronique; Guillan, Julie et al

in Microelectronic Engineering (2010, March), 87(3),

During first metal level interconnects fabrication, a controlled modification of the electro-deposited copper over-deposition (overburden) is performed using a partial chemical–mechanical polishing (CMP ... [more ▼]

During first metal level interconnects fabrication, a controlled modification of the electro-deposited copper over-deposition (overburden) is performed using a partial chemical–mechanical polishing (CMP) step. Next, copper microstructure is stabilized with a short duration hot-plate anneal. Overburden is then removed during CMP end-of-step. Ionic microscopy and EBSD observations of overburden thickness reduction reveal that copper grain growth occurs differently, according to patterned geometries and with a strong (1 1 1) texture, as observed in modified films. Reduction of overburden thickness also reveals the capacity of anneal temperature to impact electrical performances. Reliability is impacted for thinnest wires. [less ▲]

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See detailCharacterization and modeling of a scroll expander with air and ammonia as working fluid
Mendoza, Luis Carlos; Navarro-Esbrí; Bruno, Joan Carles et al

in Applied Thermal Engineering (2014), 70

This paper presents the experimental characterization and modeling of a scroll expander. The expander used here is a scroll compressor modified to work as an expander. It is characterized in two ... [more ▼]

This paper presents the experimental characterization and modeling of a scroll expander. The expander used here is a scroll compressor modified to work as an expander. It is characterized in two experimental setups using air and ammonia as working fluids. The paper studies how the main operating variables (supply pressure and temperature, pressure ratio, rotational speed and lubrication) influence the performance of the scroll expander. A semi-empirical model is proposed to determine the scroll expander performance. This model uses some semi-empirical parameters (such as built-in volume ratio, leakage area and mechanical losses), obtained through experimentation, to calculate the mechanical power, supply mass flow rate and exhaust temperature. Using this semi-empirical model, the deviations in the calculated mechanical power, exhaust temperature and supply mass flow rate are ±9%, ±4 K and ±5% Hz. [less ▲]

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See detailCharacterization and Numerical Simulation of the Jovian UV Aurora Observed with the HST Cameras
Grodent, Denis ULg; Dols, V.; Gérard, Jean-Claude ULg et al

in Science with the Hubble Space Telescope - II (1996)

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See detailCharacterization and optimization of GMO-based gels with long term release for intraarticular administration
Réeff, J.; Gaignaux, A.; Goole, J. et al

in International Journal of Pharmaceutics (2013), 451(1-2), 95-103

Osteoarthritis is characterized by slow degenerative processes in the articular cartilage within synovial joints. It could be interesting to develop a sustained-release formulation that could be effective ... [more ▼]

Osteoarthritis is characterized by slow degenerative processes in the articular cartilage within synovial joints. It could be interesting to develop a sustained-release formulation that could be effective on both pain/inflammation and restoration of mechanical integrity of the joint. Recently, an injectable system based on glycerol monooleate (GMO), containing clonidine as a model hydrophilic analgesic/anti-inflammatory drug and hyaluronic acid as a viscoelastic scaffold, showed promising potential as a biodegradable and biocompatible preparation to sustain the drug activity. However, drug release from the system is relatively fast (complete within 1 week) and the underlying drug release mechanisms not fully understood. The aims of this study were: (i) to significantly improve this type of local controlled drug delivery system by further sustaining clonidine release, and (ii) to elucidate the underlying mass transport mechanisms. The addition of FDA-approved inactive ingredients such as sodium oleate or purified soybean oil was found to be highly effective. The release rate could be substantially reduced (e.g., 50% release after 10 days), due to the increased hydrophobicity of the systems, resulting in slower and reduced water uptake and reduced drug mobility. Interestingly, Fick's second law of diffusion could be used to quantitatively describe drug release. [less ▲]

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See detailCHARACTERIZATION AND OPTIMIZATION OF PEPTIDE ENCAPSULATION IN PEGYLATED LIPOSOMES
Ducat, Emilie ULg; Brion, Michael; Evrard, Brigitte ULg et al

Poster (2009)

Purpose: The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two formulations (SPC:CHOL:mPEG-750-DSPE (47:47:6) or SPC:CHOL:mPEG-2000-DSPE (47:47:6)) were investigated ... [more ▼]

Purpose: The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two formulations (SPC:CHOL:mPEG-750-DSPE (47:47:6) or SPC:CHOL:mPEG-2000-DSPE (47:47:6)) were investigated. Methods: Blank SUV liposomes were prepared by the lipid film hydration and the encapsulation was achieved by applying freeze-thawing cycles. Because many factors may influence peptide entrapment (number of freeze-thawing cycles (NC), lipid concentration (LC), peptide concentration (PC), mixing time (MT) and liposome composition (COMP)), a design of experiment (DOE) was performed. Results: The screening permitted to identify two factors having a positive and significant influence on the encapsulation efficiencies (NC and LC) while the liposome composition had a relatively weak effect. For the second part of the DOE, the positive factors were optimized for liposomes containing mPEG2000. The obtained results revealed a theoretical optimum at 64.75±3.55% when 11 cycles were applied and for the following LC: 36.1mM SPC, 36.1mM CHOL and 4mM mPEG-2000-DSPE. Experimental results showed an encapsulation efficiency of 62.68±2.93%. Conclusion: The DOE led to significant improvement of encapsulation for liposomes containing mPEG2000. Thereafter, an optimization design for liposomes containing mPEG750 will be started. Acknowledgements: This work was supported by the Ministry of the Walloon Region. [less ▲]

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