Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detail(1)H, (13)C and (15)N assignments of a camelid nanobody directed against human alpha-synuclein.
Vuchelen, Anneleen; O'Day, Elizabeth; De Genst, Erwin et al

in Biomolecular NMR Assignments (2009), 3(2), 231-3

Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens ... [more ▼]

Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens (Kd approximately nM), making them ideal as structural probes for biomedically relevant motifs both in vitro and in vivo. We have previously shown that nanobody binding to amyloidogenic human lysozyme variants can effectively inhibit their aggregation, the process that is at the origin of systemic amyloid disease. Here we report the NMR assignments of a new nanobody, termed NbSyn2, which recognises the C-terminus of the intrinsically disordered protein, human alpha-synuclein (aS), whose aberrant self-association is implicated in Parkinson's disease. [less ▲]

Detailed reference viewed: 315 (75 ULg)
See detail1,2,4-Benzothiadiazine derivatives, their preparation and use
Pirotte, Bernard ULg; Lebrun, P.; De Tullio, Pascal ULg et al

Patent (1997)

Detailed reference viewed: 139 (10 ULg)
See detail1,2,4-Benzothiadiazine derivatives, their preparation and use
De Tullio, Pascal ULg; Nielsen, F. E.; Hansen, J. B. et al

Patent (1999)

Detailed reference viewed: 94 (4 ULg)
Full Text
Peer Reviewed
See detail1,6-AnhMurNAc derivatives for assay development of amidase AmiD.
Mercier, Frédéric ULg; Zervosen, Astrid ULg; Teller, Nathalie et al

in Bioorganic & Medicinal Chemistry (2010), 18(21), 7422-31

Various peptidoglycan fragments were synthesized from two anhydro-muramic acid derivatives protected with a Bn or a PMB group at the 4th position, in homogenate phase or on a solid support. In order to ... [more ▼]

Various peptidoglycan fragments were synthesized from two anhydro-muramic acid derivatives protected with a Bn or a PMB group at the 4th position, in homogenate phase or on a solid support. In order to facilitate HPLC detection, a chromophoric group was attached to the peptide chain. The periplasmic amidase sAmiD of Escherichia coli was used to cleave the amide bond between the lactyl group of the MurNAc and the alpha-amino group of L-Ala where the peptide chain was at least a dipeptide (L-Ala-gamma-D-Glu) amidated by benzylamine on the gamma-carboxyl group of D-Glu. In the presence of a tripeptide chain (L-Ala-gamma-D-Glu-L-Lys) or a tetrapeptide chain (L-Ala-gamma-D-Glu-m-A(2)pm-D-Ala) higher hydrolysis rates were observed. We have also demonstrated that the presence of TNB on the epsilon-amino group of L-Lys only has a small influence on the hydrolysis capacity of sAmiD. [less ▲]

Detailed reference viewed: 170 (46 ULg)
Peer Reviewed
See detail[1-11C]Bromoethane: an alternative to the use of [1-11C]iodoethane.
Schmitz, F.; Del Fiore, G.; Plenevaux, Alain ULg et al

in Journal of Labelled Compounds & Radiopharmaceuticals (1995), 37

Detailed reference viewed: 97 (4 ULg)
Full Text
See detail1-84 PTH and KDIGO Guidelines
CAVALIER, Etienne ULg

Conference (2012, April 18)

Detailed reference viewed: 111 (3 ULg)
Full Text
Peer Reviewed
See detail1-anilino-8-naphtalene sulfonate probes a gastric HK-ATPase potassium site whose access requires ionophores.
Festy, F.; Lins, Laurence ULg; Gallet, X. et al

in Journal of Membrane Biology (1998), 165(2), 153-60

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we ... [more ▼]

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we demonstrate that ANS binds to gastric membranes and probes conformational changes of the HK-ATPase independently of any active H for K exchange. Conformational changes require the presence of potassium-valinomycin and are not triggered by sodium. Potassium effect is enhanced by ATP, in the presence and in the absence of magnesium and, by ADP, in the presence of magnesium. Labeling of the pig HK-ATPase K518 by fluorescein-5-isothiocyanate inhibits the enzyme activity and knocks out the ATP effect on ANS fluorescence. Scherring 28080 and the monoclonal antibody 95-111, two competitive inhibitors of K-activated ATPase dephosphorylation, do not modify K-effect on ANS fluorescence but inhibit ATP effects. This supports that ANS does not probe K-site between the H1-H2 loop. Treatment of gastric membranes with trypsin does not inhibit the ANS response to potassium but does inhibit the response to ATP. This suggests that the ATP site inducing the ANS response is cytoplasmic and the potassium site is intramembranous. Titration reveals that one mole of ANS interacts with one mole of ATPase. We suggest that ANS probes a hydrophobic potassium site of gastric ATPase and that addition of ATP and ADP-Mg embed that site. [less ▲]

Detailed reference viewed: 128 (12 ULg)
Full Text
Peer Reviewed
See detail1-Year Controlled Randomised Trial of Prevention of Early Postmenopausal Bone Loss by Intranasal Calcitonin
Reginster, Jean-Yves ULg; Denis, D.; Albert, Adelin ULg et al

in Lancet (1987), 2(8574), 1481-3

79 women who had been menopausal for less than 36 months and who had not received any form of treatment to prevent bone loss were randomly assigned to a 12-month regimen of calcium 500 mg/day or calcium ... [more ▼]

79 women who had been menopausal for less than 36 months and who had not received any form of treatment to prevent bone loss were randomly assigned to a 12-month regimen of calcium 500 mg/day or calcium 500 mg plus intranasal salmon calcitonin 50 IU/day for 5 days per week. After 12 months of treatment bone mineral density had decreased in the calcium-only group by a mean of 3.16 (SEM 0.6)% (p less than 0.01) but had increased in the calcium plus calcitonin group by 1.38 (0.8)% (NS). The difference in response between the two treatment groups was also highly significant (p less than 0.01), as was the difference between values for hydroxyprolinuria/creatininuria (p less than 0.01). Endogenous calcitonin levels rose significantly in the calcium group but remained unchanged in calcitonin-treated patients. Treatment by calcitonin and calcium was not followed by increased secretion of parathyroid hormone. The findings suggest that intranasal calcitonin can counteract early postmenopausal bone loss. [less ▲]

Detailed reference viewed: 82 (5 ULg)
Full Text
See detail1. Implantation des cultures
Bodson, Bernard ULg; Roisin, Christian; Vancutsem, Françoise ULg et al

in Livre Blanc - Céréales-Gembloux - Informations avant les semis (2011, September 08)

Detailed reference viewed: 137 (15 ULg)
Full Text
See detail1. Etat
Lebailly, Philippe ULg; Barthelemy, P.-A.; Burny, Philippe ULg

in Hallet, C.; Ledent, A. (Eds.) Etat de l'Environnement wallon 1995, tome 2 : Agriculture (1996)

Detailed reference viewed: 375 (7 ULg)
Full Text
See detail1. Implantation des cultures
Eylenbosch, Damien ULg; Hiel, Marie-Pierre ULg; Meza Morales, Walter ULg et al

in Bodson, Bernard; Destain, Jean-Pierre (Eds.) Livre Blanc - Céréales (2014, September 11)

Detailed reference viewed: 10 (3 ULg)
Full Text
See detail1. Implantation des cultures
Bodson, Bernard ULg; Roisin, Christian; Vancutsem, Françoise ULg et al

in Livre Blanc - Céréales - Gembloux (2012, February 06)

Detailed reference viewed: 81 (4 ULg)
Full Text
See detail1. Implantation des cultures
Bodson, Bernard ULg; Vancutsem, Françoise ULg; Monfort, Bruno

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2007, September 13)

Detailed reference viewed: 137 (11 ULg)
See detail1.2 Evolution du cadre législatif et prise en charge des mineurs - Introduction
Gavray, Claire ULg

in Bailleau, Francis (Ed.) Evolution ou rupture ? La justice des mineurs en question (2008)

acts devoted to the evolutions in the European systems of justice of the minors.

Detailed reference viewed: 203 (40 ULg)
See detail10 ans du Réseau de médecins vigies. L'enregistrement de la morbidité en Belgique
Comité de rédaction IHE SSMG; Gosset, Christiane ULg

Report (1991)

Detailed reference viewed: 161 (0 ULg)