References of "Pflügers Archiv : European Journal of Physiology"
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See detailThe inositol Inpp5k 5-phosphatase affects osmoregulation through the vasopressin-aquaporin 2 pathway in the collecting system.
Pernot, E.; Terryn, S.; Cheong, S. C. et al

in Pflügers Archiv : European Journal of Physiology (2011), 462

Inositol Inpp5k (or Pps, SKIP) is a member of the inositol polyphosphate 5-phosphatases family with a poorly characterized function in vivo. In this study, we explored the function of this inositol 5 ... [more ▼]

Inositol Inpp5k (or Pps, SKIP) is a member of the inositol polyphosphate 5-phosphatases family with a poorly characterized function in vivo. In this study, we explored the function of this inositol 5-phosphatase in mice and cells overexpressing the 42-kDa mouse Inpp5k protein. Inpp5k transgenic mice present defects in water metabolism characterized by a reduced plasma osmolality at baseline, a delayed urinary water excretion following a water load, and an increased acute response to vasopressin. These defects are associated with the expression of the Inpp5k transgene in renal collecting ducts and with alterations in the arginine vasopressin/aquaporin-2 signalling pathway in this tubular segment. Analysis in a mouse collecting duct mCCD cell line revealed that Inpp5k overexpression leads to increased expression of the arginine vasopressin receptor type 2 and increased cAMP response to arginine vasopressin, providing a basis for increased aquaporin-2 expression and plasma membrane localization with increased osmotically induced water transport. Altogether, our results indicate that Inpp5k 5-phosphatase is important for the control of the arginine vasopressin/aquaporin-2 signalling pathway and water transport in kidney collecting ducts. [less ▲]

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See detailDecreased renal accumulation of aminoglycoside reflects defective receptor-mediated endocytosis in cystic fibrosis and Dent's disease.
Raggi, Claudia; Fujiwara, Kunio; Leal, Teresinha et al

in Pflügers Archiv : European Journal of Physiology (2011), 462(6), 851-60

The clinical use of aminoglycoside (AG) antibiotics is limited by their renal toxicity, which is caused by drug accumulation in proximal tubule (PT) cells. Clinical studies reported that renal clearance ... [more ▼]

The clinical use of aminoglycoside (AG) antibiotics is limited by their renal toxicity, which is caused by drug accumulation in proximal tubule (PT) cells. Clinical studies reported that renal clearance of AG is enhanced in cystic fibrosis (CF) patients, which might reflect the role of CFTR in PT cell endocytosis. In order to assess the role of chloride transporters on the renal handling of AG, we investigated gentamicin uptake and renal accumulation in mice lacking functional CFTR (Cftr ( F/F)) or knock-out for the Cl(-)/H(+) exchanger ClC-5 (Clcn5 ( Y/- )). The latter represent a paradigm of PT dysfunction and defective receptor-mediated endocytosis. As compared with controls, Cftr ( F/F) and Clcn5 ( Y/- ) mice showed a 15% to 85% decrease in gentamicin accumulation in the kidney, respectively, in absence of renal failure. Studies on primary cultures of Cftr ( F/F) and Clcn5 ( Y/- ) mouse PT cells confirmed the reduction in gentamicin uptake, although colocalization with endosomes and lysosomes was maintained. Quantification of endocytosis in PT cells revealed that gentamicin, similar to albumin, preferentially binds to megalin. The functional loss of ClC-5 or CFTR was reflected by a decrease of the endocytic uptake of gentamicin, with a more pronounced effect in cells lacking ClC-5. These results support the concept that CFTR, as well as ClC-5, plays a relevant role in PT cell endocytosis. They also demonstrate that the functional loss of these two chloride transporters is associated with impaired uptake of AG in PT cells, reflected by a decreased renal accumulation of the drug. [less ▲]

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See detailCFTR and defective endocytosis: new insights in the renal phenotype of cystic fibrosis.
JOURET, François ULg; Devuyst, Olivier

in Pflügers Archiv : European Journal of Physiology (2009), 457(6), 1227-36

Inactivation of the chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) causes cystic fibrosis (CF). Although CFTR is expressed in the kidney, no overwhelming renal phenotype is ... [more ▼]

Inactivation of the chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) causes cystic fibrosis (CF). Although CFTR is expressed in the kidney, no overwhelming renal phenotype is associated with CF. Recent studies have shown that the level of CFTR mRNA in mouse kidney approaches that found in lung. CFTR is particularly abundant in the apical area of proximal tubule cells, where it co-distributes with the Cl(-)/H(+) exchanger ClC-5 and Rab5a in endosomes. The biological relevance of CFTR in proximal tubule endocytosis has been tested in CF mouse models and CF patients. Mice lacking CFTR show a defective receptor-mediated endocytosis, as evidenced by impaired uptake of (125)I-beta(2)-microglobulin, a decreased expression of the cubilin receptor in the kidney, and a significant excretion of cubilin and its low-molecular-weight ligands into the urine. Low-molecular-weight proteinuria (and particularly transferrinuria) is similarly detected in CF patients in comparison with normal controls or patients with chronic lung inflammation. These studies suggest that the functional loss of CFTR impairs the handling of low-molecular-weight proteins by the kidney, supporting a role of CFTR in receptor-mediated endocytosis in proximal tubule cells. The selective proteinuria should be integrated in the pathophysiology of multi-systemic complications increasingly observed in CF patients. [less ▲]

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See detailPhosphoinositide phosphatases in a network of signaling reactions
Blero, D.; Payrastre, B.; Schurmans, Stéphane ULg et al

in Pflügers Archiv : European Journal of Physiology (2007), 455

Phosphoinositide phosphatases dephosphorylate the three positions (D-3, 4 and 5) of the inositol ring of the poly-phosphoinositides. They belong to different families of enzymes. The PtdIns(3,4)P(2) 4 ... [more ▼]

Phosphoinositide phosphatases dephosphorylate the three positions (D-3, 4 and 5) of the inositol ring of the poly-phosphoinositides. They belong to different families of enzymes. The PtdIns(3,4)P(2) 4-phosphatase family, the tumour suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN), SAC1 domain phosphatases and myotubularins belong to the tyrosine protein phosphatases superfamily. They share the presence of a conserved cysteine residue in the consensus CX(5)RT/S. Another family consists of the inositol polyphosphate 5-phosphatase isoenzymes. The importance of these phosphoinositide phosphatases in cell regulation is illustrated by multiple examples of their implications in human diseases such as Lowe syndrome, X-linked myotubular myopathy, cancer, diabetes or bacterial infection [less ▲]

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See detailChronic mastitis-affected cows display lower lipoxin levels than acute mastitis-affected cows
Boutet, Philippe ULg; Bureau, Fabrice ULg; Degand, Guy ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailEffects of glycopyrrolate inhalation on pulmonary function in heaves-affected horses in crisis
Art, Tatiana ULg; De Moffarts, Brieuc; Van Erck, Emmanuelle ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailCeramides play a critical role in spontaneous neutrophil apoptosis
Seumois, G.; Fillet, Marianne ULg; Gillet, Laurent ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailEffects of thiazolidinediones on tumor necrosis factor R alpha induced inflammatory cytokine expression
Desmet, Christophe ULg; Warsée, Barbara; Mélotte, D. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailSTAT5 promotes granulocyte survival during inflammation
Fievez, Laurence ULg; Desmet, Christophe ULg; Pajak, B. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailInfluence of chloride and phosphate on the binding of oxygen to canine and feline red blood cells
Cambier, Carole ULg; Wiernickx; Clerbaux, Th et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailCadmium-induced lung inflammation in rats is associated with an increase of matrix metalloproteinases-2 and -9 activity in broncho-alveolar lavage fluid
Vincke, G.; Kirschvink, Nathalie; Belleflamme, M. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailOxygen consumption and blood oxygen transport in endotoxemic calves
Cambier, Carole ULg; Clerbaux, T.; Detry, B. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailSpermine-induced modifications of intestinal urea cycle
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Pflügers Archiv : European Journal of Physiology (2004)

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See detailPolyamine as innate immunity modulator
Peulen, Olivier ULg; Powroznik, Brigitte; Gharbi, Myriam ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 449

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See detailEffect of exercise on blood glutathione in healthy race horses
De Moffarts, Brieuc; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailBiochemical findings associated with atypical myopathy in grazing horses
Votion, Dominique ULg; Delguste, Catherine ULg; Amory, Hélène ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailAnti-inflammatory effect of natural proanthocyanidins: pharmacological evaluation on in vivo models.
Garbacki, Nancy ULg; Tits, Monique ULg; Damas, Jacques ULg

in Pflügers Archiv : European Journal of Physiology (2003), 446

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See detailActivation and inactivation of bradykinin in rats.
Damas, Jacques ULg; Garbacki, Nancy ULg

in Pflügers Archiv : European Journal of Physiology (2003), 446

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See detailUse of atropine to inhibit vagally mediated baroreceptor reflex in horses undergoing dobutamine stress test
Sandersen, Charlotte ULg; Detilleux, Johann ULg; Delguste, Catherine ULg et al

in Pflügers Archiv : European Journal of Physiology (2003), 446(5), 4

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See detailEchocardiographic evaluation of cardiac performance during stimulation with dobutamine in conscious horses: a preliminary study.
Sandersen, Charlotte ULg; Delguste, Catherine ULg; Becker, Martine et al

in Pflügers Archiv : European Journal of Physiology (2002), 444(1-2), 4

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