References of "Letters in Applied Microbiology"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailGenotypic and phenotypic characterisation of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis
Bardiau, Marjorie ULg; Yamazaki, Kazuko; Duprez, Jean-Noel et al

in Letters in Applied Microbiology (2013)

Detailed reference viewed: 28 (6 ULg)
Full Text
Peer Reviewed
See detailGenotypic and phenotypic charactérisation of methicilin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis
Bardiau, M; Yamazaki, K; Duprez, Jean-Noël ULg et al

in Letters in Applied Microbiology (2013)

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailGenetic diversity and amplification of different clostridial [FeFe] hydrogenases by group-specific degenerate primers
Calusinska, Magdalena ULg; Joris, Bernard ULg; Wilmotte, Annick ULg

in Letters in Applied Microbiology (2011), 53

Aims: The aim of this study was to explore and characterize the genetic diversity of [FeFe] hydrogenases in a representative set of strains from Clostridium sp. and to reveal the existence of neither yet ... [more ▼]

Aims: The aim of this study was to explore and characterize the genetic diversity of [FeFe] hydrogenases in a representative set of strains from Clostridium sp. and to reveal the existence of neither yet detected nor characterized [FeFe] hydrogenases in hydrogen-producing strains. Methods and Results: The genomes of 57 Clostridium strains (34 different genotypic species), representing six phylogenetic clusters based on their 16S rRNA sequence analysis (cluster I, III, XIa, XIb, XIV and XVIII), were screened for different [FeFe] hydrogenases. Based on the obtained alignments, ten pairs of [FeFe] hydrogenase cluster-specific degenerate primers were newly designed. Ten Clostridium strains were screened by PCRs to assess the specificity of the primers designed and to examine the genetic diversity of [FeFe] hydrogenases. Using this approach, a diversity of hydrogenase genes was discovered in several species previously shown to produce hydrogen in bioreactors: Clostridium sartagoforme, Clostridium felsineum, Clostridium roseum and Clostridium pasteurianum. Conclusions: The newly designed [FeFe] hydrogenase cluster-specific primers, targeting the cluster-conserved regions, allow for a direct amplification of a specific hydrogenase gene from the species of interest. Significance and Impact of the Study: Using this strategy for a screening of different Clostridium ssp. will provide new insights into the diversity of hydrogenase genes and should be a first step to study a complex hydrogen metabolism of this genus. [less ▲]

Detailed reference viewed: 25 (2 ULg)
Full Text
Peer Reviewed
See detailComparison between swabbing and destructive methods for microbiological pig carcass sampling.
Ghafir, Yasmine; Daube, Georges ULg

in Letters in Applied Microbiology (2008), 47

Aims: To compare the Belgian swabbing sampling method for pig carcasses with the reference destructive method with regard to Escherichia coli and aerobic plate counts, Salmonella and Campylobacter ... [more ▼]

Aims: To compare the Belgian swabbing sampling method for pig carcasses with the reference destructive method with regard to Escherichia coli and aerobic plate counts, Salmonella and Campylobacter prevalence and their relationship. Methods and Results: Recovery was significantly lower for the swabbing method and corresponded to a recovery of 36% for E. coli counts and 81% for aerobic plate counts in comparison with the destructive method. There was no significant difference between the swabbing and destructive sampling methods for the prevalence of Salmonella or Campylobacter. A higher median for E. coli counts was detected for samples where Salmonella or Campylobacter were detected. The same association was also observed between the median for aerobic plate counts and the presence of Campylobacter. Conclusions: The method of swabbing used, covering 600 cm2 on each half-pig carcass, is efficient for the sampling of pig carcasses in comparison with the reference destructive method. Significance and Impact of the Study: This study describes an efficient method for microbiological pig carcass sampling. The Belgian swabbing method should continue to be used to allow the follow up of bacterial contamination in the Belgian meat production chain. [less ▲]

Detailed reference viewed: 37 (5 ULg)
Full Text
Peer Reviewed
See detailAnti-Listerial Activity Of Bacteriocin-Producing Lactobacillus Curvatus Cwbi-B28 And Lactobacillus Sakei Cwbi-B1365 On Raw Beef And Poultry Meat
Dortu, C.; Huch, M.; Holzapfel, Wh. et al

in Letters In Applied Microbiology (2008), 47(6),

Aim: The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw ... [more ▼]

Aim: The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. Methods and Results: The sakacin P and sakacin G structural genes were identified in Lact. curvatus CWBI-B28 and Lact. sakei CWBI-B1365 using PCR amplification, respectively. The effect of the two bacteriocinogenic strains either alone or together, and that of the nonbacteriocin-producing strain Lact. sakei LMG17302, on the growth of L. monocytogenes was evaluated in beef and poultry meat. In raw beef, the pathogenic bacteria were inhibited by the bacteriocinogenic strains. The bacteriocinogenic strains had no activity in raw chicken meat when inoculated separately, while they showed a clear anti-Listeria effect when applied together. Conclusion: Sakacin G producing Lact. sakei and sakacin P producing Lact. curvatus may be applied in raw beef to inhibit L. monocytogenes. In poultry meat, the inhibition of L. monocytogenes could only be achieved by a combined application of these bacteriocin-producing strains. Significance and Impact of the Study: In some meat products, the combined application of different class IIa bacteriocin producing lactic acid bacterium can enhance the anti-listerial activity. [less ▲]

Detailed reference viewed: 62 (6 ULg)
Full Text
Peer Reviewed
See detailEffectiveness of cell-adsorbed bacteriocin produced by Lactobacillus curvatus CWBI-B28 and selected essential oils to control Listeria monocytogenes in pork meat during cold storage.
Ghalfi, H.; Benkerroum, N.; Doguiet, D. D. K. et al

in Letters in applied microbiology (2007), 44(3),

Aims: To study the effectiveness of a combination of cell-adsorbed bacteriocin (CAB; a suspension of producer cells on which maximum bacteriocin has been immobilized by pH adjustments) of a Lactobacillus ... [more ▼]

Aims: To study the effectiveness of a combination of cell-adsorbed bacteriocin (CAB; a suspension of producer cells on which maximum bacteriocin has been immobilized by pH adjustments) of a Lactobacillus curvatus strain with oregano or savory essential oil to control Listeria monocytogenes in pork meat at 4 C. [less ▲]

Detailed reference viewed: 19 (9 ULg)
Full Text
Peer Reviewed
See detailAdherence And Colonization Properties Of Lactobacillus Rhamnosus Tb1, A Broiler Chicken Isolate
Bouzaine, T.; Dubois Dauphin, Robin ULg; Thonart, Philippe ULg et al

in Letters In Applied Microbiology (2005), 40(5),

Aims: Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A ... [more ▼]

Aims: Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A selected strain showing a good adherence in BMM test was used for in vivo colonization assays. Methods and Results: In vitro assessment of adhesion of broiler chicken isolates was performed using BMM assay. Among LAB strains tested, Lactobacillus rhamnosus TB1 showed a good adherence that was comparable to the one of an Escherichia coli EPEC strain used as positive control. For in vivo colonization assays this strain was fluorescently stained with the carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) thus allowing its detection in different layers of intestinal tract after inoculation in broiler chicken. Further, stained L. rhamnosus were found with a highest value in rectum, jejunum and ileum both 3 and 24 h after administration. Conclusions: BMM assay is a quick method to test in vitro adhesion properties of bacterial strains and cFDASE- stained bacteria may be considered as an alternative method to test in vivo adhesion and colonization properties. Significance and Impact of the Study: Lactobacillus rhamnosus TB1 was therefore showed to be able to adhere strongly in vitro to BMM and in vivo to intestinal epithelial cells of chicken and may be considered as a potential probiotic for chicken. [less ▲]

Detailed reference viewed: 47 (2 ULg)
Full Text
Peer Reviewed
See detailFluorescent Pseudomonas mainly produce the dihydro form of pyoverdine at low specific growth rate.
Jacques, Philippe ULg; Ongena, MARC ULg; Bernard, F. et al

in Letters in Applied Microbiology (2003), 36(5), 259-62

AIMS: To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent Pseudomonas strains (P. putida ... [more ▼]

AIMS: To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent Pseudomonas strains (P. putida BTP16, P. fluorescens BTP7 and P. aeruginosa 7NSK2). METHODS: PVD and dihydropyoverdine (DHPVD) productions were determined by LC ESI-MS and spectrophotometry during batch and chemostat culture at different dilution rates. SIGNIFICANCE: The relatively high PVD-to-DHPVD ratio (0.57) observed in pH-controlled batch cultures suggested that a base-catalysed chemical oxidation of the dihydroform is not the prime mechanism involved in generating PVDs. Interestingly, in chemostat cultures the PVD-to-DHPVD ratio was significantly reduced at low specific growth rate. Our results suggest that the oxidation of DHPVD to PVD is catalysed by an iron-dependent enzymatic reaction rather than a chemical oxidation. [less ▲]

Detailed reference viewed: 26 (2 ULg)
Peer Reviewed
See detailEffect of water extract of Moringa oleifera seeds on the hydrolytic microbial species diversity of a UASB reactor treating domestic wastewater.
Kalogo, Y.; Rosillon, Françis ULg; Hammes, F. et al

in Letters in Applied Microbiology (2000), 31(3), 259-264

The effect of a continuous supply of a water extract of Moringa oleifera seeds (WEMOS) on the hydrolytic microbial population of biomass grown in mesophilic upflow anaerobic sludge blanket reactors ... [more ▼]

The effect of a continuous supply of a water extract of Moringa oleifera seeds (WEMOS) on the hydrolytic microbial population of biomass grown in mesophilic upflow anaerobic sludge blanket reactors treating domestic wastewater was investigated. The WEMOS-treated sludge had seemingly a wider diversity, with enterobacter and klebsiella as dominant hydrolytic bacteria, compared with the control sludge. Additional tests indicated that various hydrolytic bacteria could degrade WEMOS. It appeared that a continuous supply of WEMOS to an anaerobic digester, treating domestic wastewater, increased the diversity of hydrolytic bacteria and therefore enhanced the biological start-up of the reactor. [less ▲]

Detailed reference viewed: 39 (3 ULg)
Full Text
Peer Reviewed
See detailMedium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the γ-decalactone production by Yarrowia lipolytica
Waché, Y.; Bergmark, K.; Courthaudon, J.-L. et al

in Letters in Applied Microbiology (2000), 30(3), 183-187

Size of methyl ricinoleate droplets during biotransformation into γ-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium ... [more ▼]

Size of methyl ricinoleate droplets during biotransformation into γ-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d 32 of 2.5 μm whereas it was 0.7 μm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 μm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions. [less ▲]

Detailed reference viewed: 9 (1 ULg)