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See detailDensity-Dependent Reproductive Success In Tribolium Castaneum (Herbst) (Coleoptera : Tenebrionidae)
Assie, L. K.; Brostaux, Yves ULg; Haubruge, Eric ULg

in Journal of Stored Products Research (2008), 44(3), 285-289

Two strains of Tribolium castaneum, one being susceptible to malathion (Asm) and the other malathion-specific resistant (PRm), were used to assess the effect of population density and its interaction with ... [more ▼]

Two strains of Tribolium castaneum, one being susceptible to malathion (Asm) and the other malathion-specific resistant (PRm), were used to assess the effect of population density and its interaction with genetic background in reproductive success. A highly significant allometric relationship between female body weight and fecundity (R2=0.413) and another between female body weight and larval survivorship (R2=0.561) were found. Data showed that population density exerted an indirect effect on the reproductive success. The body weight of the Asm female was not affected by variations in population density but the body weight of the PRm female increased with reduced population density. The genetic background (strain) and/or malathion-specific resistance greatly influenced reproductive success. Concerning the percentage survivorship of offspring of the two strains, there was an opposite trend with increased population density: in PRm, survivorship was lower at high density (76.64±13.75; mean±SD) and higher at low density (88.39±7.61), whereas in Asm, survivorship was higher at high density (53.39±15.57) and lower at low density (43.99±20.08). The PRm female laid more eggs than the Asm. Reproductive success was significantly higher in PRm than in Asm. In addition, the genetic background (the strain) and/or the pleiotropic effect of malathion-specific resistance had a significant effect on the fecundity of PRm because the differences in fecundity and reproductive success between Asm and PRm went beyond the body weight of the female and might be explained by their genetic background and/or the pleiotropic effect of malathion-specific resistance. [less ▲]

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See detailResponse and genetic analysis of malathion-specific resistant Tribolium castaneum (Herbst) in relation to population density
Assie, L. K.; Francis, Frédéric ULg; Gengler, Nicolas ULg et al

in Journal of Stored Products Research (2007), 43(1), 33-44

Extensive use of malathion for pest control on stored cereals has resulted in worldwide resistance in red flour beetles, Tribolium castaneum. In this paper we investigate population density effects on the ... [more ▼]

Extensive use of malathion for pest control on stored cereals has resulted in worldwide resistance in red flour beetles, Tribolium castaneum. In this paper we investigate population density effects on the malathionspecific resistance in PRm, a strain from the Philippines, in an integrated resistance management framework. Two populations of malathion-specific resistant (PRm) and a malathion- susceptible strain of T castanewn were bred at different densities: low (4 adults/g) and high (12 adults/g) density on wheat plus brewer's yeast in the laboratory. After eight generations, slopes of probit regression lines and LC50 values were used to monitor the effect of insect rearing density on the progression of malathion-specific resistance. The LC50 of the malathion- susceptible strain (Asm) did not change significantly during selection while LC(50)s varied for both the high-density and low-density lines of PRm, the LC50 of malathion ranged from 27.51 to 34.06 and from 21.14 to 29.39 Vg malathion cm(-2) for high and low density, respectively. More than 33 generations were required to achieve a 10-fold increase of resistance for the low-density line compared to only 17 generations for the high-density line. Calculations from published formulae suggested that the malathion-specific resistance of both high- and low-density lines was under monofactorial control, with complete dominance. The data showed that environmental factors such as population density differences in insect rearing and development may influence the heritability of resistance. Furthermore, the variability in results published worldwide on resistance emphasises the need to standardize test conditions across laboratories. (c) 2005 Published by Elsevier Ltd. [less ▲]

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See detailEfficacy of diatomaceous earth formulations admixed with grain against populations of Tribolium castaneum
Arnaud, Ludovic; Lan, H. Tran Thi; Brostaux, Yves ULg et al

in Journal of Stored Products Research (2005), 41(2), 121-130

The efficacy of diatomaceous earth (DE) to control stored-products Coleoptera on stored grain was examined against several populations of the red flour beetle, Tribolium castaneum (Herbst). Four ... [more ▼]

The efficacy of diatomaceous earth (DE) to control stored-products Coleoptera on stored grain was examined against several populations of the red flour beetle, Tribolium castaneum (Herbst). Four commercially available DE formulations were tested: INSECTO(R), Perma-Guard(TM), Protect-It(R) and the diatomite used for the production of Dryacide(R), each at six concentrations (100-1000ppm). A great variation of efficacy was observed among the DE formulations tested. Protect-It at concentrations up to 400 ppm was found to be the most effective formulation to control red flour beetle populations. However, a concentration of 1000 ppm of Protect-It was necessary to control all adults of all populations. Most T castaneum populations, except one from Ivory Coast (Asm), were more than 90% controlled with INSECTO and Dryacide DE at 600 ppm. At this concentration, about 88% and 22% Asm adults died with INSECTO and Dryacide DE, respectively. Perma-Guard was the least efficient DE formulation to control T castaneum adults with three populations exhibiting some survival at 1000 ppm. Reduced susceptibility to DE was observed in two populations, Asm and Lab susceptible from Kansas (Lab-S). As neither population had been previously exposed to DE, it is suggested that red flour beetles may naturally vary in susceptibility to DE. In addition, it was found that some populations can be satisfactorily controlled with some DE formulations but not with others. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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See detailEffect of ice-nucleating bacteria (Pseudomonas syringae Van Hall) on insect susceptibility to sub-zero temperatures
Mignon, Jacques ULg; Haubruge, Eric ULg; Gaspar, Charles ULg

in Journal of Stored Products Research (1998), 34(1), 81-86

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits ... [more ▼]

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits the acclimation of insects and enhances their cold tolerance. The objective of this study was to examine the influence of the concentration of the ice-nucleating-active bacterium Pseudomonas syringae (10, 100 and 1000 ppm), temperature and duration of sub-zero exposure on the cold tolerance of the granary weevil Sitophilus granarius (L.) and the saw-toothed grain beetle Oryzaephilus surinamensis (L.). After an application of 1000 ppm of powdered P. syringae to grain, the mortality of S. granarius and O. surinamensis was increased after 24-h exposure to -4 degrees C. Higher mortality was observed after exposure to colder temperatures and a dose-response relationship was evident. At near-zero (-4 to 0 degrees C) negative temperatures, no dose response was observed, and the mortality in treated grain was the same as that in untreated grain. (C) 1998 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailThe impact of sperm precedence in malathion resistance transmission in populations of the red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)
Haubruge, Eric ULg; Arnaud, Ludovic; Mignon, Jacques ULg

in Journal of Stored Products Research (1997), 33(2), 143-146

Malathion resistance in the red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) is actually a worldwide problem, and studies on resistance transmission are needed to improve ... [more ▼]

Malathion resistance in the red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) is actually a worldwide problem, and studies on resistance transmission are needed to improve insecticide resistance management. Females of Tribolium castaneum commonly mate with several males, and the last batch of male sperm preferentially fertilizes subsequent eggs. This phenomenon, a particular form of sexual selection, helps to increase resistance transmission in populations of stored product insects. We confirmed the last male sperm precedence and, in the absence of further matings, examined the evolution of mixed susceptible and malathion-resistant progeny during a 3-month period. The proportion of resistant phenotypes in female progeny was 99.6 and 3%, respectively, after the first mating with a resistant male and the second mating with a susceptible one. When females thus mated twice were isolated from males, the proportion of the resistant phenotype increased to 34.1% after 30 days. From 72 days onwards, this proportion ranged from 14.2 to 29.7%. (C) 1997, Published by Elsevier Science Ltd. [less ▲]

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See detailBiological-Activity Of Fenoxycarb Against Sitophilus-Zeamais Motsch (Coleoptera, Curculionidae)
Letellier, C.; Haubruge, Eric ULg; Gaspar, Charles ULg

in Journal of Stored Products Research (1995), 31(1),

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