References of "Journal of Insect Physiology"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailRole of long-chain hydrocarbons in the aggregation behaviour of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Durieux, Delphine ULg; Fischer, Christophe ULg; Brostaux, Yves ULg et al

in Journal of Insect Physiology (2012)

The multicoloured Asian ladybeetles, Harmonia axyridis (Pallas), form large aggregations inside dwellings to survive cold winters, causing annoyances to householders from their number and sometimes the ... [more ▼]

The multicoloured Asian ladybeetles, Harmonia axyridis (Pallas), form large aggregations inside dwellings to survive cold winters, causing annoyances to householders from their number and sometimes the induction of allergic reactions. Migratory flight and macrosite choice of this species is well documented. H. axyridis shows a hypsotactic behaviour and a clear preference for contrasting visual elements. However, how the microsite is selected remains undocumented, although a better understanding of the implicated factors could lead to the development of new control methods for this pest. In this work, we have hypothesized that non-volatile compounds are involved in the microsite choice and the aggregation process of this beetle. Long chain hydrocarbons were identified inside aggregation sites, comprising saturated and unsaturated homologues. An aggregation bioassay was then conducted on overwintering individuals, highlighting the retention capacity of the previously cited compounds on the tested ladybeetles. Additional investigations have shown that H. axyridis males and females, originating from overwintering sites, deposit a similar blend of molecules while walking. A Y-shaped tube assay revealed that this blend is used by male and female congeners as cue, allowing individuals to orientate towards the treated side of the olfactometer. These results suggest the use of two different blends of long chain hydrocarbons by H. axyridis during its aggregative period, the first one to lead conspecifics towards aggregation sites (microsites) and the second to ensure the cohesion of the aggregation. These findings support the potential use of these blends, in association with volatiles, in the design of traps in order to control infestations of this species in dwellings. [less ▲]

Detailed reference viewed: 70 (19 ULg)
Full Text
Peer Reviewed
See detailTritrophic interactions among Macrosiphum euphorbiae aphids, their host plants and endosymbionts: investigation by a proteomic approach.
Francis, Frédéric ULg; Guillonneau, F.; Leprince, Pierre ULg et al

in Journal of Insect Physiology (2010), 56(6), 575-85

The Mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (Macrosiphum euphorbiae). This study used 2D-DIGE coupled with protein identification by MALDI-TOF-MS to compare the ... [more ▼]

The Mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (Macrosiphum euphorbiae). This study used 2D-DIGE coupled with protein identification by MALDI-TOF-MS to compare the proteome patterns of avirulent and semivirulent potato aphids and their bacterial endosymbionts on resistant (Mi-1.2+) and susceptible (Mi-1.2-) tomato lines. Avirulent aphids had low survival on resistant plants, whereas the semivirulent clone could colonize these plants. Eighty-two protein spots showed significant quantitative differences among the four treatment groups, and of these, 48 could be assigned putative identities. Numerous structural proteins and enzymes associated with primary metabolism were more abundant in the semivirulent than in the avirulent aphid clone. Several proteins were also up-regulated in semivirulent aphids when they were transferred from susceptible to resistant plants. Nearly 25% of the differentially regulated proteins originated from aphid endosymbionts and not the aphid itself. Six were assigned to the primary endosymbiont Buchnera aphidicola, and 5 appeared to be derived from a Rickettsia-like secondary symbiont. These results indicate that symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance. [less ▲]

Detailed reference viewed: 74 (19 ULg)
Full Text
Peer Reviewed
See detailAnti-predator defence mechanisms in sawfly larvae of Arge (Hymenoptera, Argidae)
Petre, Charles-Albert ULg; Detrain, Claire; Boevé, Jean-Luc

in Journal of Insect Physiology (2007), 53

Detailed reference viewed: 13 (2 ULg)
Full Text
Peer Reviewed
See detailDifferential sensitivity of two insect GABA-gated chloride channels to dieldrin, fipronil and picrotoxinin
Le Corronch, Hervé; Alix, Philippe ULg; Hue, B

in Journal of Insect Physiology (2002), 48

In the central nervous system of both vertebrates and invertebrates inhibitory neurotransmission is mainly achieved through activation of gamma-aminobutyric acid (GABA) receptors. Extensive studies have ... [more ▼]

In the central nervous system of both vertebrates and invertebrates inhibitory neurotransmission is mainly achieved through activation of gamma-aminobutyric acid (GABA) receptors. Extensive studies have established the structural and pharmacological properties of vertebrate GABA receptors. Although the vast majority of insect GABA-sensitive responses share some properties with vertebrate GABAA receptors, peculiar pharmacological properties of these receptors led us to think that several GABA-gated chloride channels are present in insects. We describe here the pharmacological properties of two GABA receptor subtypes coupled to a chloride channel on dorsal unpaired median (DUM) neurones of the adult male cockroach. Long applications of GABA induce a large biphasic hyperpolarization, consisting of an initial transient hyperpolarization followed by a slow phase of hyperpolarization that is not quickly desensitized. With GABA, the transient hyperpolarization is sensitive to picrotoxinin, fipronil and dieldrin whereas the slow response is insensitive to these insecticides.When GABA is replaced by muscimol and cis-4-aminocrotonic acid (CACA) a biphasic hyperpolarization consisting of an initial transient hyperpolarization followed by a sustained phase is evoked which is blocked by picrotoxinin and fipronil. Exposure to dieldrin decreases only the early phase of the muscimol and CACA-induced biphasic response, suggesting that two GABA-gated chloride channel receptor subtypes are present in DUM neurones. This study describes, for the first time, a dieldrin resistant component different to the dieldrin- and picrotoxinin-resistant receptor found in several insect species. [less ▲]

Detailed reference viewed: 16 (2 ULg)