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See detailEffect of γ-radiation on free radicals formation, structural changesand functional properties of wheat starch
Atrous, Hager; Benbettaieb, Nasreddine; Hosni, Faouzi et al

in International Journal of Biological Macromolecules (2015), 80

Wheat starch was treated by different γ-radiation doses (3, 5, 10, 20, 35 and 50 kGy). The effects of γ-radiation on structural, thermal, physicochemical, morphological and rheological properties of wheat ... [more ▼]

Wheat starch was treated by different γ-radiation doses (3, 5, 10, 20, 35 and 50 kGy). The effects of γ-radiation on structural, thermal, physicochemical, morphological and rheological properties of wheat starch were studied. The presence of free radicals after γ-radiation treatment, which number decreased with time was confirmed. Structural analysis revealed decreases in the intensities of the O–H and C–H stretches and glycosidic linkages indicating the depolymerization of amylose and probably amy-lopectin into shorter chain molecules, but showed that γ-radiation treatment did not affect the crystalline structure. Differential scanning calorimetric (DSC) thermograms showed the absence of significant differences in the gelatinization temperatures, as well as the corresponding transition enthalpies since the DSC parameters are related to the crystalline ordering within the granules. Apparent amylose content decreased linearly with increasing irradiation dose leading to an increase in water solubility index. Anincrease in the swelling power was observed after irradiation treatment until 20 kGy, followed by a rapiddecrease at higher doses. Microscopic observations showed that the effect of γ-radiation was more visible on starch pastes than on starch granules. Rheological properties of the starch pastes decreased within creasing irradiation dose as a result of glycosidic bond cleavage. [less ▲]

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See detailStructural, functional, and ACE inhibitory properties of water-solublepolysaccharides from chickpea flours
Mokni Ghribi, abir; Sila, Assaâd; Maklouf Gafsi, Ines et al

in International Journal of Biological Macromolecules (2015), 75

tThe present study aimed to characterize and investigate the functional and angiotensin-I convertingenzyme (ACE) inhibition activities of chickpea water-soluble polysaccharides (CPWSP). Physico ... [more ▼]

tThe present study aimed to characterize and investigate the functional and angiotensin-I convertingenzyme (ACE) inhibition activities of chickpea water-soluble polysaccharides (CPWSP). Physico-chemicalcharacteristics were determined by nuclear magnetic resonance spectroscopy (NMR), Fourier transform-infrared spectroscopy (FT-IR) analysis, and X-ray diffractometry (XRD). Functional properties (waterholding capacity: WHC, water solubility index: WSI, swelling capacity: SC, oil holding capacity: OHC,foaming, and emulsion properties) and ACE activities were also investigated using well-established pro-cedures. The FT-IR spectra obtained for the CPWSP revealed two significant peaks, at about 3500 and500 cm−1, which corresponded to the carbohydrate region and were characteristic of polysaccharides.All spectra showed the presence of a broad absorption between 1500 and 670 cm−1, which could beattributed to C−H, C−O, and O−H bands in the polysaccharides. CPWSP had an XRD pattern that wastypical for a semi-crystalline polymer with a major crystalline reflection at 19.6◦C. They also displayedimportant techno-functional properties (SWC, WSI, WHC, and OHC) that can be modulated accordingto temperature. The CPWSP were also noted to display good anti-hypertensive activities. Overall, theresults indicate that CPWSP have attractive chemical, biological, and functional properties that makethem potential promising candidates for application as alternative additives in various food, cosmetic,and pharmaceutical preparations. [less ▲]

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See detailImpact of glycerol and nitrogen concentration on Enterobacter A47 growth and exopolysaccharide production
Torres, Ch.; Marques, R.; Ferreira, A.R. et al

in International Journal of Biological Macromolecules (2014), 71

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See detailCharacterization of sugar beet pectic-derived oligosaccharides obtained by enzymatic hydrolysis.
Combo, Agnan Marie Michel ULg; Aguedo, Mario ULg; Quiévy, N et al

in International Journal of Biological Macromolecules (2013), 52(1), 148-156

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See detailComparison of chitosan/siRNA and trimethylchitosan/siRNA complexes behaviour in vitro
Dehousse, Vincent ULg; Garbacki, Nancy ULg; Jaspart, Séverine ULg et al

in International Journal of Biological Macromolecules (2010), 46

Chitosan and trimethylchitosan (TMC)-siRNA nanoparticles were produced by simple complexation technique or by ionic gelation using tripolyphosphate (TPP). The obtained complexes were characterized in ... [more ▼]

Chitosan and trimethylchitosan (TMC)-siRNA nanoparticles were produced by simple complexation technique or by ionic gelation using tripolyphosphate (TPP). The obtained complexes were characterized in terms of physicochemical properties such as size, zeta potential, complexation efficiency and stability. Furthermore, cytotoxicity, cell uptake and transfection efficiency of polyplexes were evaluated in vitro. Under pH condition of cell culture medium, a strong decrease in siRNA condensation efficiency was observed with chitosan nanoparticles. This characteristic resulted in low transfection efficiencies in HEK293 cell line. Formulation of chitosan polyplexes with TPP led to improvement of polyplexes stability but no significant increase in transfection efficiency was observed compared to simple chitosan complexes. By contrast, TMC complexes did not have pH dependency on siRNA complexation. TMCsiRNA nanoparticles were stable in physiological condition. Accordingly, cellular uptake was increased compared to chitosan polyplexes. However, improvement of transfection efficiency was low regarding to cellular uptake of these complexes. Chitosan and TMC complexes present some characteristics favourable for siRNA delivery, such as ability to integrate siRNA into small discrete particles or low toxicity of the complexes. This study also highlights the importance of complexes stability in physiological environment for siRNA transfection purposes. [less ▲]

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See detailPresence of histone H1 and the electric birefringence behavior of nucleosome fractions from sucrose gradient and column chromatography
Houssier, Claude ULg; Hacha, renée; De Pauw-Gillet, Marie-Claire ULg et al

in International Journal of Biological Macromolecules (1981), 3(1), 3159-62

Differences in H1 content and electric birefringence properties are observed in chromatin fractions and can explain tail folding against the nucleosome core.

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