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See detailMeasuring the general phytosanitary situation: development of a plant health barometer
Wilmart, Olivier; Van Huffel, Xavier; Diricks, Herman et al

in European Journal of Plant Pathology (2014), Online First Articles

The development of a plant health barometer, an instrument to measure the general phytosanitary situation on a national level (Belgium) and on a yearly basis and to monitor its evolution over time, is ... [more ▼]

The development of a plant health barometer, an instrument to measure the general phytosanitary situation on a national level (Belgium) and on a yearly basis and to monitor its evolution over time, is described. The elaboration of a set of 13 plant health indicators (PHI’s) as the basis for the plant health barometer is discussed. These indicators were weighted by experts - including scientists, policy makers and agroindustrial representatives - to determine their relative impact in the barometer. The result of the barometer is expressed as a comparison with the previous year. Based on the results of the 13 PHI’s, it is concluded that the general plant health in Belgium shows a positive evolution from 2007 until 2010 and a negative evolution from 2010 until 2012. The plant health barometer provides a overview of the phytosanitary situation of plants and plant products in Belgium and is a tool to communicate in an intelligible, comprehensible manner on aspects of plant health to consumers and professional stakeholders in the plant production chain. Together with the food safety barometer and the animal health barometer, the plant health barometer is one of the three instruments to provide a overview on the general status of the safety of the food chain in Belgium. [less ▲]

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See detailcDNA-AFLP analysis of gene expression changes in apple trees induced by phytoplasma infection during compatible interaction
Aldaghi, Majid; Bertaccini, Assunta; Lepoivre, Philippe ULg

in European Journal of Plant Pathology (2012), 134

Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment ... [more ▼]

Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique was used. A rootstock of apple (MM106) susceptible to ‘Ca. P. mali’ was used to extend the range of the potential host responses by the maximum number of identified genes that will be deregulated by phytoplasma in apple. Gene expression comparisons were studied in three directions: healthy versus infected samples, symptomatic versus nonsymptomatic sample, and AP-infected versus ATinfected sample. Forty-five genes whose steady-state levels of expression significantly changed in response to phytoplasma infection were identified. Among their partial cDNA sequences, only 27 showed similarity to DNA or protein data bases; of these, 18 were related to known genes in plants, and the rest were related to unknown or hypothetical proteins. Eighteen out of 45 did not show any similarity with sequences in data bases (potential novel genes). Quantitative real-time RT-PCR (qRT-PCR) was used to confirm differential expression of AFLP identified genes, and showed the similar profile expression for 11 known genes among 18, and for 13 unknown, hypothetical or novel genes among 27. Changes in gene expression involved a wide spectrum of biological functions, including processes of metabolism, cell defence, senescence, photosynthesis, transport, transcription, signal transduction and protein synthesis. This is the first study of global gene profiling in plants in response to phytoplasma infections using cDNA-AFLP, and a model is proposed to explain the mode of action of the ‘Ca. P. mali’ in apple. [less ▲]

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See detailAssessment Of Pichia Anomala (Strain K) Efficacy Against Blue Mould Of Apples When Applied Pre- Or Post-Harvest Under Laboratory Conditions And In Orchard Trials
Lahlali, R.; Massart, Sébastien ULg; De Clercq, D. et al

in European Journal of Plant Pathology (2009), 123(1), 37-45

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See detailStudy of bacterial determinants involved in the induction of systemic resistance in bean by Pseudomonas putida BTP1
Ongena, MARC ULg; Giger, A.; Jacques, Philippe ULg et al

in European Journal of Plant Pathology (2002), 108(3), 187-196

The ability of Pseudomonas putida BTP1 to induce resistance in bean to Botrytis cinerea was demonstrated in soil experiments on plants pre-inoculated at the root level with the bacteria before challenge ... [more ▼]

The ability of Pseudomonas putida BTP1 to induce resistance in bean to Botrytis cinerea was demonstrated in soil experiments on plants pre-inoculated at the root level with the bacteria before challenge with the leaf pathogen. As a first step to characterize the molecules from BTP1 responsible for induction of systemic resistance in bean, heat-killed cells and supernatant from culture in an iron-limited medium were tested for their protective effect. Most of the resistance-eliciting activity of the strain was retained in the crude cell-free culture fluid. In vivo assays with samples from successive fractionation steps of the BTP1 supernatant led, (i) to the conclusion that salicylic acid, pyochelin and pyoverdin, previously identified as Pseudomonas determinants for induced systemic resistance (ISR), were not involved in systemic resistance triggered by BTP1, and (ii) to the isolation of fractions containing one main metabolite that retained most of the resistance-inducing activity in bean. Although this molecule remains to be structurally characterized, its isolation is an addition to the range of determinants from plant growth-promoting rhizobacteria (PGPR) known to stimulate plant defences. [less ▲]

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See detailRapid And Homogenous Detection Of Apple Stem Pitting Virus By Rt-Pcr And A Fluorogenic 3 ' Minor Groove Binder-Dna Probe
Salmon, Marc ULg; Vendrame, Marina; Kummert, Jean et al

in European Journal of Plant Pathology (2002), 108(8), 755-762

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See detailCultural characteristics, pathogenicity and vegetative compatibility of Fusarium udum isolates from pigeonpea (Cajanus Cajan (L.) Millsp.) in Kenya
Kiprop, E. K.; Mwang'Ombe, A. W.; Baudoin, Jean-Pierre ULg et al

in European Journal of Plant Pathology (2002), 108(2), 147-154

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