  cDNA-AFLP analysis of gene expression changes in apple trees induced by phytoplasma infection during compatible interaction; ; Lepoivre, Philippe  in European Journal of Plant Pathology (2012), 134 Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment ... [more ▼] Abstract In order to gain insight into molecular and physiological changes in apple trees during compatible interaction with two ‘Candidatus Phytoplasma mali’ strains (AP and AT), cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique was used. A rootstock of apple (MM106) susceptible to ‘Ca. P. mali’ was used to extend the range of the potential host responses by the maximum number of identified genes that will be deregulated by phytoplasma in apple. Gene expression comparisons were studied in three directions: healthy versus infected samples, symptomatic versus nonsymptomatic sample, and AP-infected versus ATinfected sample. Forty-five genes whose steady-state levels of expression significantly changed in response to phytoplasma infection were identified. Among their partial cDNA sequences, only 27 showed similarity to DNA or protein data bases; of these, 18 were related to known genes in plants, and the rest were related to unknown or hypothetical proteins. Eighteen out of 45 did not show any similarity with sequences in data bases (potential novel genes). Quantitative real-time RT-PCR (qRT-PCR) was used to confirm differential expression of AFLP identified genes, and showed the similar profile expression for 11 known genes among 18, and for 13 unknown, hypothetical or novel genes among 27. Changes in gene expression involved a wide spectrum of biological functions, including processes of metabolism, cell defence, senescence, photosynthesis, transport, transcription, signal transduction and protein synthesis. This is the first study of global gene profiling in plants in response to phytoplasma infections using cDNA-AFLP, and a model is proposed to explain the mode of action of the ‘Ca. P. mali’ in apple. [less ▲] Detailed reference viewed: 20 (0 ULg)Assessment Of Pichia Anomala (Strain K) Efficacy Against Blue Mould Of Apples When Applied Pre- Or Post-Harvest Under Laboratory Conditions And In Orchard Trials; Massart, Sébastien ; et alin European Journal of Plant Pathology (2009), 123(1), 37-45 Detailed reference viewed: 25 (3 ULg)Inter-Laboratory Evaluation Of A Duplex RT-PCR Method Using Crude Extracts For The Simultaneous Detection Of Prune Dwarf Virus And Prunus Necrotic Ringspot VirusMassart, Sébastien ; Brostaux, Yves ; et alin European Journal of Plant Pathology (2008), 122(4), 539-547 Detailed reference viewed: 31 (8 ULg) Study of bacterial determinants involved in the induction of systemic resistance in bean by Pseudomonas putida BTP1Ongena, MARC ; ; et alin European Journal of Plant Pathology (2002), 108(3), 187-196 The ability of Pseudomonas putida BTP1 to induce resistance in bean to Botrytis cinerea was demonstrated in soil experiments on plants pre-inoculated at the root level with the bacteria before challenge ... [more ▼] The ability of Pseudomonas putida BTP1 to induce resistance in bean to Botrytis cinerea was demonstrated in soil experiments on plants pre-inoculated at the root level with the bacteria before challenge with the leaf pathogen. As a first step to characterize the molecules from BTP1 responsible for induction of systemic resistance in bean, heat-killed cells and supernatant from culture in an iron-limited medium were tested for their protective effect. Most of the resistance-eliciting activity of the strain was retained in the crude cell-free culture fluid. In vivo assays with samples from successive fractionation steps of the BTP1 supernatant led, (i) to the conclusion that salicylic acid, pyochelin and pyoverdin, previously identified as Pseudomonas determinants for induced systemic resistance (ISR), were not involved in systemic resistance triggered by BTP1, and (ii) to the isolation of fractions containing one main metabolite that retained most of the resistance-inducing activity in bean. Although this molecule remains to be structurally characterized, its isolation is an addition to the range of determinants from plant growth-promoting rhizobacteria (PGPR) known to stimulate plant defences. [less ▲] Detailed reference viewed: 20 (5 ULg)Rapid And Homogenous Detection Of Apple Stem Pitting Virus By Rt-Pcr And A Fluorogenic 3 ' Minor Groove Binder-Dna ProbeSalmon, Marc ; ; et alin European Journal of Plant Pathology (2002), 108(8), 755-762 Detailed reference viewed: 27 (3 ULg)Cultural Characteristics, Pathogenicity And Vegetative Compatibility Of Fusarium Udum Isolates From Pigeonpea (Cajanus Cajan (L.) Millsp.) In Kenya; ; Baudoin, Jean-Pierre et alin European Journal of Plant Pathology (2002), 108(2), 147-154 Detailed reference viewed: 25 (1 ULg)Molecular And Pathogenicity Characteristics Of Phytophthora Nicotianae Responsible For Root Necrosis And Wilting Of Pepper (Capsicum Annuum L.) In Tunisia; Lepoivre, Philippe in European Journal of Plant Pathology (2000), 106(9), Detailed reference viewed: 11 (1 ULg)Virulence Variation And Rapd Polymorphism In African Isolates Of Phaeoisariospis Griseola (Sacc.) Ferr., The Causal Agent Of Angular Leaf Spot Of Common Bean; Jijakli, Mohamed ; Lepoivre, Philippe in European Journal of Plant Pathology (1999), 105(6), Detailed reference viewed: 12 (1 ULg)Correlation Between Hydrolytic Enzyme Activities Measured In Bean Seedlings After Trichoderma Koningii Treatment Combined With Pregermination And The Protective Effect Against Pythium Splendens; Lepoivre, Philippe ; in European Journal of Plant Pathology (1996), 102(5), Detailed reference viewed: 3 (0 ULg) |
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