Regional variability in stratum corneum reactivity to antiseptic formulations.
Quatresooz, Pascale ; Xhauflaire, Emmanuelle ; Pierard, Claudine et al
in Contact Dermatitis (2007), 56(5), 271-3
Skin does not react in an identical way to the action of chemicals over all anatomic sites. Accordingly, distinct regional differences have been described in relation to irritancy. The present study ... [more ▼]
Skin does not react in an identical way to the action of chemicals over all anatomic sites. Accordingly, distinct regional differences have been described in relation to irritancy. The present study assesses the regional variations of stratum corneum (SC) reactivity to 3 proprietary antiseptic solutions (povidone iodine (PVP-I), 70 mg/ml and 100 mg/ml, and chlorhexidine digluconate 50 mg/ml) using the corneoxenometry (CXM) bioassay. SC was harvested from the volar forearm, the forehead and the back in 30 young adults. Each SC sample was covered by one of the neat test product or deionized water for 2 hr at 20 degrees C. The intrinsic staining property of each antiseptic on SC was assessed by reflectance colorimetry. For the CXM bioassay, samples were then stained by a toluidine blue-basic fuschin solution in order to show protein denaturation induced by the test products. The colorimetric index of mildness (CIM = L*- Chroma C*) was measured by colorimetry. Data show that PVP-I 100 mg/ml was the least reactive antiseptic to the SC. It was significantly milder than the 2 other antiseptics. [less ▲]Detailed reference viewed: 20 (1 ULg)
Skin capacitance imaging and corneosurfametry. A comparative assessment of the impact of surfactants on stratum corneum.
Xhauflaire, Emmanuelle ; ; et al
in Contact Dermatitis (2006), 54(5), 249-53
Silicon image sensor (SIS) technology was recently introduced as an innovative tool (SkinChip, L'Oreal) providing sensitive imaging of the skin capacitance. This method can detect discrete focal ... [more ▼]
Silicon image sensor (SIS) technology was recently introduced as an innovative tool (SkinChip, L'Oreal) providing sensitive imaging of the skin capacitance. This method can detect discrete focal variations in skin surface hydration, and thus early discrete manifestations of skin irritation induced by surfactants. In the present in vivo study, 2 neat and diluted shampoos, and 5% and 10% sodium laurylsulfate solutions were tested on human skin. Each surfactant solution was gently rubbed on the skin using wet hair wicks mimicking the casual use of a shampoo on the scalp. Clinical and SIS evaluations were carried out. In addition, the same products were tested using the ex vivo corneosurfametry bioassay performed on human stratum corneum (SC) harvested by cyanoacrylate skin surface strippings. The colourimetric index of mildness (CIM) was measured on these samples. The product reactivity with the SC was recognized by darker skin capacitance images, and by both lowered SkinChip-generated values and lowered CIM values. The extent in changes varied according to the nature of the test products and their concentrations. The SkinChip image changes likely corresponded to the acute surfactant-induced water swelling of the corneocytes. Skin capacitance imaging and corneosurfametry allow to disclose discrete surfactant-induced alterations of corneocytes. [less ▲]Detailed reference viewed: 83 (0 ULg)
Contact and photocontact allergy to ketoprofen. The Belgian experience.
; ; et al
in Contact Dermatitis (2004), 50(4), 238-241
Topical ketoprofen (KP) is widely used because of its anti-inflammatory effect. However, photocontact dermatitis is a side-effect. Between May 2001 and June 2002, the Belgian Contact & Environmental ... [more ▼]
Topical ketoprofen (KP) is widely used because of its anti-inflammatory effect. However, photocontact dermatitis is a side-effect. Between May 2001 and June 2002, the Belgian Contact & Environmental Dermatitis Group conducted a prospective, open patch and photopatch test study in 20 patients suspected of KP dermatitis. Severe skin symptoms requiring systemic corticotherapy occurred in 47%. 5 patients were hospitalized. 1 patient showed prolonged photosensitivity. All patients were tested with KP and the other constituents of KP gel. Attribution to KP was demonstrated in all cases. Patch and photopatch tests with KP 2% in petrolatum showed contact photoallergy in 17 patients, contact allergy in 1 patient and photoaggravated contact allergy in 2 patients. 5 patients also reacted to the fragrance components lavender (Lavandula augustifolia) oil and/or neroli (Citrus aurantium dulcis) oil 5% in alcohol. However, in 4 of these, irritant reactions to the ethanolic dilutions could not be ruled out. Additional tests with 3 non-steroidal anti-inflammatory drugs without benzophenone structure ibuprofen, naproxen and diclofenac identified only 1 contact allergic reaction to diclofenac. Cross-reactivity to the substituted benzophenones, oxybenzone and sulisobenzone occurred only to the first in less than 30% of the patients. A high frequency (69%) of contact allergy to fragrance mix was found. Dermatologists should be aware of the severity of photoallergic reactions to KP and the risk of cross-sensitization. [less ▲]Detailed reference viewed: 12 (0 ULg)
Responsive corneosurfametry following in vivo skin preconditioning.
Uhoda, Emmanuelle ; Goffin, Véronique ; Pierard, Gérald
in Contact Dermatitis (2003), 49(6), 292-6
Skin is subjected to many environmental threats, some of which altering the structure and function of the stratum corneum. Among them, surfactants are recognized factors that may influence irritant ... [more ▼]
Skin is subjected to many environmental threats, some of which altering the structure and function of the stratum corneum. Among them, surfactants are recognized factors that may influence irritant contact dermatitis. The present study was conducted to compare the variations in skin capacitance and corneosurfametry (CSM) reactivity before and after skin exposure to repeated subclinical injuries by 2 hand dishwashing liquids. A forearm immersion test was performed on 30 healthy volunteers. 2 daily soak sessions were performed for 5 days. At inclusion and the day following the last soak session, skin capacitance was measured and cyanoacrylate skin-surface strippings were harvested. The latter specimens were used for the ex vivo microwave CSM. Both types of assessments clearly differentiated the 2 hand dishwashing liquids. The forearm immersion test allowed the discriminant sensitivity of CSM to increase. Intact skin capacitance did not predict CSM data. By contrast, a significant correlation was found between the post-test conductance and the corresponding CSM data. In conclusion, a forearm immersion test under realistic conditions can discriminate the irritation potential between surfactant-based products by measuring skin conductance and performing CSM. In vivo skin preconditioning by surfactants increases CSM sensitivity to the same surfactants. [less ▲]Detailed reference viewed: 23 (0 ULg)