References of "Communications in Agricultural and Applied Biological Sciences"
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See detailPotential demal exposure of florists to fungicide residues on flowers and risk assessment
Toumi; Joly, L.; Vleminckx, C. et al

in Communications in Agricultural and Applied Biological Sciences (in press)

Flowers are susceptible to many pests and diseases. Therefore, they can be sprayed several times during their growth considering that no MRL are set for flow-ers. High levels of pesticide residues ... [more ▼]

Flowers are susceptible to many pests and diseases. Therefore, they can be sprayed several times during their growth considering that no MRL are set for flow-ers. High levels of pesticide residues potentially expose daily the florists who han-dle cut flowers and possibly could endanger their health. A study was carried out to evaluate the risk for florists exposed to fungicide residues during normal profes-sional tasks. Cotton gloves were distributed to 20 florists (two pairs to each florist) and worn during two consecutive half days during normal professional tasks (from min 2 hours to max 3 hours/day) to measure their potential dermal exposure (PDE). Samples were analyzed with a multi-residue (QuEChERS) method validated by a laboratory accredited for pesticide residues and with a combination of gas and liquid chromatography tandem mass spectrometry. It appears from the results that a total of 54 fungicides with different toxicity classes were detected on cotton gloves. An average of 15.53 mg/kg fungicide residues per glove sample was meas-ured. Six of 54 are suspected of causing cancer after prolonged or repeated expo-sure. Boscalid was both the active substance for which the highest maximum and average concentrations (26.21 and 3.47 mg/kg, respectively). Famoxadone had the most critical PDE (156% AOEL for the maximum concentration). As a consequence, this study leads to conclude that Belgian florists, who worked for several years and handled a large number of flowers contaminated by high concentrations of pesti-cide residues, are exposed daily with a potential effect on their health. This sug-gests that safety standards should be set for residue levels on cut flowers. [less ▲]

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See detailRisk assessment for small farmers exposed to plant protection products in the Niger River valley
Massalatchi Illyassou, Karimoun; Adamou, R.; Schiffers, Bruno ULg

in Communications in Agricultural and Applied Biological Sciences (in press)

Plant protection products play a very important role in agriculture. However, their misuse can have serious negative impacts both on human health and environment. A study was carried out to identify the ... [more ▼]

Plant protection products play a very important role in agriculture. However, their misuse can have serious negative impacts both on human health and environment. A study was carried out to identify the plant protection products used in the Niger River valley and to observe the local pesticide management practices. Ten active substances were identified as the most used chemicals by farmers. Their toxicological properties were characterized and their respective Potential Dermal Exposures (PDE) was evaluated in order to assess the risk level for the local small growers. The UK Predictive Operator Exposure Model was used to quantify the PDE during mixing/loading and application according to the local practices. The survey shows that the most common active substances are organophosphate or pyrethroids insecticides. In addition, some other prohibited and counterfeit pesticides cocktails are also used. All active substances used in Niger River valley are highly toxic. When sprayed without personal protective equipment (PPE) they could induce significant harmful impacts on the human health after exposure. The predictive exposure levels vary from 0.0013 mg/kg bw/day to 0.4125 mg/kg bw/day, several times higher the Acceptable Operator Exposure Level (AOEL) for all actives substances. The survey also revealed that 76% of operators do not use any PPE during mixing/loading or spraying. Other bad practices observed in study area can increase the exposure of operators. Moreover, local consumers could also be exposed through intake of pesticide residues on harvested products. [less ▲]

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See detailEfficacy of Basil-Cabbage intercropping to control insect pests in Benin, West Africa
Yarou, Boni Barthélémy ULg; Assogba Komlan, Françoise; Tossou, Eric et al

in Communications in Agricultural and Applied Biological Sciences (in press)

Cabbage (Brassica oleracea L.) is a common vegetable whose production is severely limited by insect pest pressure in Western Africa. This study was conducted during the dry and rainy season in the south ... [more ▼]

Cabbage (Brassica oleracea L.) is a common vegetable whose production is severely limited by insect pest pressure in Western Africa. This study was conducted during the dry and rainy season in the south Benin to evaluate the potential of tropical basil (Ocimum gratissimum L.) for repelling cabbage pests (Hellula undalis Fabricius, Plutella xylostella L. and Spodoptera litto-ralis Boisduval). In a complete randomized block design with four replicates, the insect infestation were compared between three association modalities: (i) cabbage intercropped with tropical basil, (ii) cabbage plots surrounded by tropical basil and (iii) control (no basil in the cabbage area). The presence of tropical basil near cabbage plots significantly reduced insect pest abundance on cabbage. Damages were also less important on cabbage intercropped with tropical basil. Among both intercropping modalities, alternate rows showed the best results in terms of reducing pest populations and damages than compared to cabbage plots surrounded by tropical basil. [less ▲]

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See detailComparison of explant responses treated with leachate and leonardite sources of humic substances during in vitro rooting of woody plants.
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Communications in Agricultural and Applied Biological Sciences (2016), 81(1), 158-165

As heterogeneous mixtures of compounds resulting from the physical, chemical and microbiological transformations of organic residues, humic substances (HS) are mostly recognized for their biostimulation ... [more ▼]

As heterogeneous mixtures of compounds resulting from the physical, chemical and microbiological transformations of organic residues, humic substances (HS) are mostly recognized for their biostimulation of plant growth that firstly involve the root development and architecture before further putative improvement of nutrients uptakes. To avoid the interferences currently reported from external origins, the successive steps of rooting have been carried out using shoots and isolated leaves of birch and alder vitro-plants. Extracts issued from landfill leachate (LHS) has been compared to a stable formulation from leonardite ("Humifirst" 12% humic acid 3% and fulvic acid) commercialized by TRADECORP company's (HHS). Chemical analysis showed that LHS source typically contain much higher N (mainly as ammonium (93%) and chloride concentration than HHS. Used at low concentration (10 ppm) during root induction/initiation phase, both HS sources may be slightly unfavorable to the root formation (21% of reduction in primary root number) of alder but not of birch. While, in root elongation phase, there is an increase in the primary root length and lateral root number. The direct effects of HS on in vitro root development vary from one species to another depending on the root treatment stage. Results showed that both explants type response are equivalent in the development of a complete rooting system. [less ▲]

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See detailLINKING CATTLE GRAZING BEHAVIOR TO METHANE AND CARBON DIOXIDE DYNAMICS
Blaise, Yannick ULg; Lebeau, Frédéric ULg; Andriamandroso, Andriamasinoro ULg et al

in Communications in Agricultural and Applied Biological Sciences (2016, February), 81(1), 107-112

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well ... [more ▼]

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well as addressing the selection of low producing individuals. On pasture and in the barn, variations in CH4 emissions are observed depending on the time of the day. However, no studies have been made to link these diurnal fluctuations to behavioural phases, especially on pasture. The aim of this study was to understand the individual dynamics of CH4 production and their links to the grazing behaviour. For this purpose, a new tool was specifically developed. Five red-pied dry cows were equipped with infrared CH4 and carbon dioxide (CO2) sensors measuring concentrations in the exhaled air at 4 Hz. The animals were equipped with a heart rate belt (HR) and motion sensors to detect their feeding behaviours (grazing vs. rumination) for periods of 8 h/d. Wind speed (WS) was also monitor to verify interference with sampled gas concentrations. Results showed that using the CH4:CO2 ratio reduced the interference with WS that was observed on raw CH4 and CO2 concentration signals. CH4:CO2 ratio average over 5 min periods indicated that CH4 emissions were lower during grazing than rumination (P<0.01). The eructation frequency during grazing (0.48 eructation/min, P<0.01) was also lower than during rumination (0.65 eructation/min). HR was higher during grazing that rumination. Because HR is usually linked to metabolic CO2 production intensity, hence influencing the denominator of the CH4:CO2 ratio, further investigation should focus on the quantification of changes in fermentative and metabolic CO2 emissions along the day to estimate total CH4 production more accurately and the relationship between CH4 emissions patterns and post-feeding times. [less ▲]

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See detailFirst survey on prevalence and infestation rates of Varrroa mite in Gabon
Fabre Anguilet, Edgard ULg; Alabi, Taofic ULg; Bengone Ndong, Toussaint et al

in Communications in Agricultural and Applied Biological Sciences (2016), 81(3), 299-305

Varroa mites cause considerable damage to Apis mellifera Linnaeus colonies in North America, Europe and Asia. To control this parasite, beekeepers in these parts of world have to use chemical acaricides ... [more ▼]

Varroa mites cause considerable damage to Apis mellifera Linnaeus colonies in North America, Europe and Asia. To control this parasite, beekeepers in these parts of world have to use chemical acaricides. In Africa, this pest has been identified, and survey of Varroa infestation rates showed a lot of variation across honey bee sub-species. Generally, African colonies seemed to be resistant or tolerant to the presence of Varroa mite. The objective of our study is to explore the presence, prevalence and infestation rates of Varroa mite in Gabon. The presence and quantitative assessment of Varroa mites were performed in 55 wild colonies of Apis mellifera adansonii Latreille in two locations. Our results showed that: (1) 70% and 48% of the wild colonies studied were infested with Varroa destructor according to locations, (2) Varroa infestation rate was lower than 0.5 mite per 100 bees regardless location. The infestation rates obtained were still very low compared to those observed in various other regions in Africa. In perspective, it would be interesting to explore the reasons that could explain the low infestation rates which were observed. [less ▲]

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See detailA survey of pesticide residues in cut flowers from various countries
Toumi, Khaoula ULg; Vleminckx, Christiane; Van Loco, Joris et al

in Communications in Agricultural and Applied Biological Sciences (2016), 81(3), 493-502

As in any intensive culture, flowers require the use of a wide range of pesticides to control diseases and pests which can damage production and marketability. In order to evaluate the average levels of ... [more ▼]

As in any intensive culture, flowers require the use of a wide range of pesticides to control diseases and pests which can damage production and marketability. In order to evaluate the average levels of contamination of the cut flowers and to assess the risk for professionals exposed to pesticide residues when handling cut flowers, a survey was carried out with a group of florists from the Belgian largest cities. Fifty samples of roses (5 stems per bouquet) were collected: 45 bouquets were sampled in the 7 largest cities of Belgium (Antwerp, Brussels, Charleroi, Ghent, Leuven, Liege and Namur) and 5 were sampled from 5 supermarkets. Analysis of residual pesticide deposit is made by combining two multi-residue methods (GC-MS-MS and LC-MS-MS) in a laboratory accredited for pesticide residues. For all the samples analysed, a total of 97 active substances were detected, i.e. an average of 14 active substances per bouquet and a total average pesticide load of 26,03 mg/kg per flower sample. Most active substances (a.s.) reached high levels of residues, with concentrations between 10 and 50 mg/kg. Samples from Belgium and The Netherlands have a lower average number of a.s./sample, but the amount of residues is about the same in all samples (20-30 mg/kg) whatever the country of origin , except for the sample from Germany who is the worst case (22 a.s. with a total amount of 92 mg/kg). Most of the detected active substances are fungicides (dodemorph, spiroxamine, cyprodinil, fluopyram, pyrimethanil, benomyl (carbendazim), propamocarb, boscalid and iprodione) which are present on more than 20 of the 50 samples. All of them have a dermal acute toxicity. Consequently, florists who handle a large number of flowers are exposed daily with a potential effect on their health. [less ▲]

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See detailComparison of efficiency and selectivity of three bio-insecticides for the protection of tomatoes in Burkina Faso
Son, Diakalia ULg; Somda, I.; Legrève, Anne et al

in Communications in Agricultural and Applied Biological Sciences (2016), 81(3), 289-297

The search for less toxic products and alternative methods is a need to elaborate integrated control strategies for growing tomatoes in Burkina Faso to reduce the dependence of farmers on pesticides. A ... [more ▼]

The search for less toxic products and alternative methods is a need to elaborate integrated control strategies for growing tomatoes in Burkina Faso to reduce the dependence of farmers on pesticides. A trail was carried out in the village of Koula, from December 2015 to March 2016, to assess the effectivveness and action spectrum of biopesticides on several key pests of tomato (Solanum lycopersicum L.) in Burkina Faso, namely two Lepidoptera caterpillars (Helicoverpa armigera (Hub.) and Utetheisa pulchella L.) and whiteflies (Bemisia tabaci (Genn.)). The effectiveness of the three products already sold in the country has been evaluated: Bio K 16 (1.5 kg/ha), H-N (3 L/ha) and PiOL (3 L/ha). In the aim to cover the spectrum of pests, and thus coming closer to integrated Pest Management practical conditions, eache biopesticides has been applied in association with abamectine, an insecticide-acaricide (ALCARIUS 18 EC, 1 L/ha). In general, all treatments resulted in significant protection of the tomato fruit against caterpillars of moths, but had no effect on Bemisia tabaci. The combination Bacillus thuringiensis-abamectine hasprovided both the best protection of the fruits and the highest yield (19.6 T/ha). This combination could be recommended as part of IPM in tomato cultivation. [less ▲]

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See detailEVALUATION OF THERMOTOLERANT ACETOBACTER PASTEURIANUS STRAINS ISOLATED FROM MOROCCAN FRUITS CATALYZING OXIDATIVE FERMENTATION AT HIGH TEMPERATURE.
Mounir, Majid ULg; Shafiei, R.; Zarmehrkhorshid, R. et al

in Communications in Agricultural and Applied Biological Sciences (2015), 80(1), 37-43

Six strains of acetic acid bacteria were isolated from Moroccan local products and their potential as industrial strains was evaluated in lab-bioreactor. Three of them, namely TAV01, AF01 and CV01 ... [more ▼]

Six strains of acetic acid bacteria were isolated from Moroccan local products and their potential as industrial strains was evaluated in lab-bioreactor. Three of them, namely TAV01, AF01 and CV01, isolated from traditional apple vinegar, apple and cactus fruit, respectively were selected and their responses to high temperature were assessed. Morphological and biochemical identification confirmed that these strains belong to Acetobacter species. Their growth and acetic acid production were compared with the thermoresistant reference strain, Acetobacter senegalensis and mesophilic strains of Acetobacter pasteurianus. The two strains AF01 and CV01 showed abundant growth and noticeable acetic acid production ability at high temperatures (38 to 41 degrees C). A thermophilic character was observed for AF01 strain. Indeed, this bacterium grew better at 38 than 30 degrees C. [less ▲]

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See detailIMPLEMENTATION OF A METAL STRUCTURED PACKING IN A FUNGAL BIOFILM REACTOR FOR THE PRODUCTION OF A RECOMBINANT PROTEIN BY ASPERGILLUS ORYZAE
Zune, Quentin ULg; Delepierre, Anissa; Toye, Dominique ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07)

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See detailUsing micro-injection technique to assess fungal toxicity in mosquito control
Bawin, Thomas ULg; Boukraa, Slimane; Seye, Fawrou et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07), 79(1), 181-185

Topical application of insecticidal compounds allows directly exposing these substances on insect tissues and measuring their toxicity while ignoring many factors. However, this technique remains ... [more ▼]

Topical application of insecticidal compounds allows directly exposing these substances on insect tissues and measuring their toxicity while ignoring many factors. However, this technique remains difficult to apply on mosquito larvae considering their aquatic lifestyle. Micro-injection could be used for the direct deposition of toxic compounds in the larvae. Capillaries exhibiting an injection tip with an external diameter of 0.5 mm have been designed from silica tubes. For each treatment, a capillary is mounted on a pump connected to a flow rate regulator. Culex quinquefasciatus larvae were injected with 10^7 spores/ml of entomopathogenic fungi (Aspergillus clavatus, Metarhizium anisopliae, Metarhizium sp.). Mortalities were recorded daily during 72h. The distribution of spores stained with methylene blue and injected into the body of larvae was also observed according to the system described. Results showed that spores were distributed over the whole body. The injection of Aspergillus clavatus, Metarhizium anisopliae and Metarhizium sp spores induced corrected mortalities of 62%, 53% and 57% after 72h, and differed statistically from control groups. Finally, post-mortem emergences of filaments from dead larvae were observed in the case of the three fungal strains confirming spore viability. Injection of inactivated spores (or inert bodies of similar size) could help to reject the hypothesis of a response due to the presence of foreign bodies. [less ▲]

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See detailPhysico-chemical properties and aroma profile of Acacia Honey produced in Romania
Madas, Mariana-Niculina ULg; Francis, Frédéric ULg; Marghitas, L.A. et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07), 79(1), 133-135

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See detailDevelopment of a colorimetric method for the dosage of OI- anions and I2 in aqueous media
Bafort, Françoise ULg; Barthelemy, Jean-Paul ULg; Parisi, Olivier ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(1), 155-160

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See detailA volatile sex pheromone in the invasive ladybeetle Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Fassotte, Bérénice ULg; Fischer, Christophe ULg; Durieux, Delphine ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(1), 79-81

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See detailYearly Follow-up of Methane Turbulent Exchange Over an Intensively Grazed Pasture in Belgium
Dumortier, Pierre ULg; Aubinet, Marc ULg; Beckers, Yves ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(1), 91-96

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See detailAssessing the foraging behavior of Agriotes sordidus wireworms in dual-choice olfactometers
Barsics, Fanny ULg; Fiers, Marie; Fauconnier, Marie-Laure ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(2), 151-156

The different steps of the foraging process of wireworms (Coleoptera: Elateridae) would be better understood if accurate and holistic information regarding the role of plant-produced chemicals ... [more ▼]

The different steps of the foraging process of wireworms (Coleoptera: Elateridae) would be better understood if accurate and holistic information regarding the role of plant-produced chemicals constituting their environment were available. Volatile organic compounds (VOC) play important roles in the interactions between plants and insects in many ecosystems, whether they take place aboveground or belowground. The roles of VOC are still relatively unknown for wireworms, and deserve attention. Here, we performed three experimentations with barley roots as baits. In the two first, we assessed the effect of chopped roots and fungus infected roots on the orientation of wireworms. In the third experiment, the larvae were confronted to both healthy and fungus infected roots. We discuss the results in terms of suitability of the olfactometers we designed for the investigation of olfaction in wireworms, and we provide suggestions to improve their use. [less ▲]

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See detailSilky bent grass resistance to herbicides: one year of monitoring in Belgium
Henriet, François; Bodson, Bernard ULg; Meza Morales, Walter ULg

in Communications in Agricultural and Applied Biological Sciences (2013, May 21), 78(3), 665-670

Silky bent grass (Apera spica-venti (L.) P. Beauv.) is a common weed of cereal crops widely spread in Northern and Easthern Europe (Germany, Czech Republic, ...), Northern Asia, Sibera and Canada. Up to ... [more ▼]

Silky bent grass (Apera spica-venti (L.) P. Beauv.) is a common weed of cereal crops widely spread in Northern and Easthern Europe (Germany, Czech Republic, ...), Northern Asia, Sibera and Canada. Up to now, no resistant case has been detected in Belgium but some chemical weeding failures have been observed in Wallonia fields. During summer 2011, 37 seed samples of Apera spica-venti were collected in Wallonia and submitted to resistance tests in controlled conditions. Three modes of action were tested: acetyl coenzyme-A carboxylase inhibitors (pinoxaden and cycloxydim), acetolactate synthase inhibitores (mesosulfuron+iodosulfuron, pytroxsulam and sulfometuron) and photosynthesis inhibitors (isoproturon). One susceptible standard population was included in the test in order to validate it and to permit wild populations classification according to "R" rating system developed by Moss et al (2007). Most of populations were susceptible but some populations showed resistance to at least one of the three tested modes of action. [less ▲]

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See detailHigh-speed imaging use to predict spray retention on barley leaves
Boukhalfa, Hassina dite Hafida ULg; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

in Communications in Agricultural and Applied Biological Sciences (2013, May 21), Vol 78(2)(1-386 (2013)), 31-36

Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera ... [more ▼]

Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera coupled with a retro-LED lighting. Size and velocity of the drop were extracted by image analysis. Drop impact types were determined by the operator. Drops were produced with a flat-fan nozzle mounted on a movable ramp. Two surfactants (Break-Thru® S240 and Li700 ®) were sprayed on BBCH 12 barley leaves to highlight the effect of the reduction of surface tension. Relative volume proportions were computed within of an energy scale divided into 11 classes based on the Weber number. results are compared to the results of the chemical analysis by spectrofluerometry. [less ▲]

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See detailDevelopment of an experimental device allowing plant-plant interaction studies and in situ dynamic trapping of volatile organic compounds emitted by barley (Hordeum distichon L.) roots
Delory, Benjamin ULg; Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February 08), 78(1), 97-102

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly ... [more ▼]

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly performed on aboveground plant tissues, leaving the roles played by root VOCs in plant−plant interaction unexplored. In this context, this project aims at setting up an original experimental device allowing both dynamic trapping of VOCs emitted by mechanically damaged H. distichon roots and the study of the roles played by root VOCs in intra and interspecific plant−plant interactions. The experimental device consists of Barley seedlings cultivated in closed PTFE reactors filled with wet sand. Before being analysed by gas chromatography−mass spectrometry, root VOCs are trapped via a dynamic system on Tenax cartridges using a charcoal-filtered and humidified air. Preliminary results show that 7 day-old wounded Barley roots emit C9 fatty acid derivatives (E-non-2-enal and nona-2,6-dienal) as major compounds, contrasting with aboveground plant tissues that mainly emit C6 alcohols, aldehydes, and their derivative esters. For plant−plant interaction studies, receiver plants are exposed to an airflow enriched with VOCs from root damaged Barley plants of the same age. [less ▲]

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See detailLong term measurements of volatile organic compounds exchanges above a maize field at Lonzee (Belgium)
Bachy, Aurélie ULg; Aubinet, Marc ULg; SALERNO, Giovanni ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February), 78(1), 127-132

VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role ... [more ▼]

VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role in the atmospheric chemistry and their final impact on air pollution and climate change. Evaluation of current and future VOC emissions is thus necessary and requires a comprehensive understanding of VOC production and exchange dynamics under a wide panel of climatic conditions and ecosystems. Forest and non pastured grasslands have been largely studied for the last decade. However, knowledge about VOC fluxes from croplands remains scarce. Our study focuses on the VOC exchanges between a maize field and the atmosphere. It is incorporated in a wider project that aims to study VOC fluxes from two croplands (maize and winter wheat) and a pastured grassland. VOC fluxes have been measured on a maize field during the whole growing season using a micrometeorological method (eddy covariance). While first results show half-hourly bidirectionnal exchanges among all the preselected compounds, in average methanol stands for the greatest emitted VOC, followed by green leaf volatiles, and acetic acid is the greatest taken up VOC. Small isoprene and monoterpenes fluxes are also observed. A diurnal pattern is found for all those VOC, with greater emission/uptake during the day, suggesting a flux dependence on environmental parameters. These environmental controls will be further investigated [less ▲]

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