Clinical recognition and aspects of the cerebral folate deficiency syndromes

in Clinical Chemistry & Laboratory Medicine (2012)

Aberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report

in Clinical Chemistry & Laboratory Medicine (2012), 50(3), 583-584

Elevation of cardiac and oxidative stress biomarkers after a running activity in sedentary subjects

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(Special Suppl), 313

Vitamin D: clinical relevance, analytical issues

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(s1), 66

Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 265-70

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human ... [more ▼]

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance of this new assay was evaluated. Clinical relevance was determined by comparison with a reference method in a cohort of kidney transplant patients. Patients and methods: First, the analytical performance of the Binding Site cystatin C kit was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy. Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in our population of renal transplantation. Conclusions: The use of algorithms based on cystatin C and creatinine could provide a reliable estimate of GFR in kidney transplantation. [less ▲]

Analytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 271-275

Neutrophil gelatinase-associated lipocalin (NGAL) determined in urine with the Abbott Architect or in plasma with the Biosite Triage? The laboratory's point of view

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 339-341

Multicentre evaluation of the Tosoh HbA1c G8 analyser

in Clinical Chemistry & Laboratory Medicine (2010), 48(3), 365-371

A new statistical method for evaluating long-term analytical performance of laboratories applied to an external quality assessment scheme for flow cytometry.

in Clinical Chemistry & Laboratory Medicine (2010), 48(5), 645-50

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the ... [more ▼]

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the target and reported values of each parameter for each laboratory during the past 3 years. This study aims to develop a method to find laboratories with aberrant variability or bias using robust techniques and to obtain robust estimates of the variability. METHODS: A method is proposed to find outliers with respect to the individual regression line, followed by a step to find regression lines with excessive variability and finally a step to find regression lines with high bias. RESULTS: The model was applied to the results obtained by 52 laboratories for CD4%. From the 1340 data points, 35 were determined to be regression outliers. The second step revealed one regression line with excessive variability; the third step detected three regression lines with exceeding bias. CONCLUSIONS: The methodology allows assessment of the long-term performance of laboratories, taking into account samples with different target values. Outliers in the first step indicate accidental mistakes, outliers in the second and third step point to high analytical variability or bias. [less ▲]

VALIDATION AND ACCURACY PROFILES OF THE LIAISON BAP_OSTASE (DIASORIN), A NEW METHOD FOR THE DETERMINATION OF BONE ALKALINE PHOSPHATASE

in Clinical Chemistry & Laboratory Medicine (2009, June), 47(51),