Multicopy suppression screen in a Saccharomyces cerevisiae strain lacking the Rab GTPase-activating protein Msb3p.

in Biotechnology Letters (2011), 33(1), 123-9

Culture And Spray-Drying Of Tsukamurella Paurometabola C-924: Stability Of Formulated Powders

in Biotechnology Letters (2007), 29(11),

The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 628C ... [more ▼]

The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 628C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage condition was at 48C under vacuum. A new method for the calculation of cell death order for bacteria stored at low temperatures was developed. Powders stored under vacuum showed an Arrhenius behavior in relation to cell death kinetics. [less ▲]

Expression of alternansucrase in potato plants

in Biotechnology Letters (2007), 29

Alternan, which consists of alternating alpha(1 - 3)/alpha (1 - 6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides ... [more ▼]

Alternan, which consists of alternating alpha(1 - 3)/alpha (1 - 6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g–1 fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch- synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico- chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [alpha (1 - 3)-linked glucosyl residues and dextran [alpha (1 - 6)-linked glucosyl residues]. [less ▲]

The Tre2 Oncoprotein, Implicated In Ewing'S Sarcoma, Interacts With Two Components Of The Cytoskeleton

in Biotechnology Letters (2006), 28(4),

Multicopy Suppression Screen In The Msb3 Msb4 Saccharomyces Cerevisiae Double Mutant, Affected In Ypt/Rabgap Activity

in Biotechnology Letters (2005), 27(19),

Alkaloid accumulation in Catharanthus roseus cell suspension cultures fed with stemmadenine

in Biotechnology Letters (2004), 26(10), 793-798

Feeding stemmadenine to Catharanthus roseus cell suspension culture resulted in the accumulation of catharanthine, tabersonine and condylocarpine. Condylocarpine is not an intermediate in the pathway to ... [more ▼]

Feeding stemmadenine to Catharanthus roseus cell suspension culture resulted in the accumulation of catharanthine, tabersonine and condylocarpine. Condylocarpine is not an intermediate in the pathway to catharanthine or tabersonine when it is fed to the cultures. The results support the hypothesis that stemmadenine is an intermediate in the pathway to catharanthine and tabersonine. [less ▲]

A recombinant beta-O-glucosidase from Caldocellum saccharolyticum to hydrolyse desulfo-glucosinolates

in Biotechnology Letters (2001), 23(6), 443-446

Glucosinolates, natural compounds found in Brassicaceae, can easily be transformed into desulfo-glucosinolates by action of Helix pomatia sulfatase. The recombinant beta -O-glucosidase from Caldocellum ... [more ▼]

Glucosinolates, natural compounds found in Brassicaceae, can easily be transformed into desulfo-glucosinolates by action of Helix pomatia sulfatase. The recombinant beta -O-glucosidase from Caldocellum saccharolyticum does not catalyse glucosinolate degradation but can hydrolyse desulfo-glucosinolates (thio-D-glucosidic substrates) to produce the corresponding pure nitriles, including valuable homochiral representatives. [less ▲]

Conversion of green note aldehydes into alcohols by yeast alcohol dehydrogenase

in Biotechnology Letters (1999), 21(7), 629-633

'Green note' aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into ... [more ▼]

'Green note' aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into (Z)-3-hexenol, Pichia anomala gave the best results. Conversion yields higher than 90% were also obtained by directly conducting the reaction in the medium where (Z)-3 -hexenal is produced by the action of lipoxygenase and hydroperoxide lyase on linolenic acid. [less ▲]

Electrical microenvironment influence on the hydrolytic activity of free and immobilised Yarrowia lipolytica lipase

in Biotechnology Letters (1996), 18(1), 73-78

The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia lipolytica lipase has been investigated. Sodium taurocholate and Sedipur 400, an anionic polyacrylamide ... [more ▼]

The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia lipolytica lipase has been investigated. Sodium taurocholate and Sedipur 400, an anionic polyacrylamide, enhance the negative character of the fatty droplets of substrate and tend to improve the lipolytic activity while the cationic polyacrylamide (Sedipur 900) has opposite effects. Ca+ which reduces the fatty droplets charge as Sedipur 900, is however a good activator of the enzyme. The role of electrical properties on the optimum pH of the immobilised enzyme is clearer. Immobilisation of the lipase on a positively charged support shifts its optimum pH to acidic pH by repulsion towards H+ ions around the support. [less ▲]

OPTIMIZATION OF LIPASE-CATALYZED SYNTHESIS OF CITRONELLYL ACETATE IN SOLVENT-FREE MEDIUM

in Biotechnology Letters (1994), 16(7), 693-696

Citronellol esterification catalyzed by Candida antarctica lipase is performed with high yields (74%) in a solvent-free medium. The use of dessicants gives a 10% yield enhancement and substrate excess ... [more ▼]

Citronellol esterification catalyzed by Candida antarctica lipase is performed with high yields (74%) in a solvent-free medium. The use of dessicants gives a 10% yield enhancement and substrate excess consumption by adding calculated amounts of acetic acid lead to a 80% pure citronellyl acetate. Quantities up to 100 ml are treated with success. [less ▲]