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See detailUpscaling of an electronic nose for continuously stirred tank reactor stability monitoring from pilot-scale to real-scale agricultural co-digestion biogas plant
Adam, Gilles ULg; Lemaigre, Sébastien; Goux, Xavier et al

in Bioresource Technology (in press)

This study investigated the use of an electronic nose for on-line anaerobic reactor state monitoring at the pilot-scale level and then upscaling to the full-scale level. E-nose indicator was compared to ... [more ▼]

This study investigated the use of an electronic nose for on-line anaerobic reactor state monitoring at the pilot-scale level and then upscaling to the full-scale level. E-nose indicator was compared to classical state indicators such as pH, alkalinity, volatile fatty acids concentration and to other gas phase compounds. Multivariate statistical process control method, based on principal component analysis and the Hotelling's T² statistics was used to derive an indicator representative of the reactor state. At the pilot-scale level, the e-nose indicator was relevant and could distinguish 3 process states: steady-state, transient and collapsing process. At the full-scale level, the e-nose indicator could provide the warning of the major disturbance whereas two slight disturbances were not detected and it gave one major false alarm. This work showed that gas phase relation with anaerobic process should be deeper investigated, as an e-nose could indicate the reactor state, focusing on the gas phase. [less ▲]

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See detailImproving effect of metal and oxide nanoparticles encapsulated in porous silica on fermentative biohydrogen production by Clostridium butyricum.
Beckers, Laurent; Hiligsmann, Serge ULg; Lambert, Stéphanie ULg et al

in Bioresource Technology (2013), 133C

This paper investigated the enhancement effect of nanometre-sized metallic (Pd, Ag and Cu) or metallic oxide (Fe(x)O(y)) nanoparticles on fermentative hydrogen production from glucose by a Clostridium ... [more ▼]

This paper investigated the enhancement effect of nanometre-sized metallic (Pd, Ag and Cu) or metallic oxide (Fe(x)O(y)) nanoparticles on fermentative hydrogen production from glucose by a Clostridium butyricum strain. These nanoparticles (NP) of about 2-3nm were encapsulated in porous silica (SiO(2)) and were added at very low concentration (10(-6)molL(-1)) in batch hydrogen production test. The cultures containing iron oxide NP produced 38% more hydrogen with a higher maximum H(2) production rate (HPR) of 58% than those without NP or with silica particles only. The iron oxide NP were used in a 2.5L sequencing-batch reactor and showed no significant effect on the yields (established at 2.2mol(hydrogen)mol(glucose)(-1)) but an improvement of the HPR (+113%, reaching a maximum HPR of 86mL(hydrogen)L(-1)h(-1)). These results suggest an improvement of the electron transfers trough some combinations between enzymatic activity and inorganic materials. [less ▲]

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See detailProduction of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.
Bechet, Max; Castera-Guy, Joany; Guez, Jean-Sebastien et al

in Bioresource technology (2013), 145

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin ... [more ▼]

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin producer. Compared to the parental strain, both mutants overproduced constitutively mycosubtilin, while BBG125 had lost the ability to synthesize surfactin. Surprisingly, BBG125 was found to produce about 2-fold less mycosubtilin than BBG116 despite an expected higher availability of the cytoplasmic precursors and cofactors pool for biosynthesis. Further physiological characterization of BBG125 also highlighted: (i) a strong influence of temperature on mycosubtilin biosynthesis in BBG125 with a maximal productivity observed at 22 degrees C, compared to 15 and 30 degrees C; (ii) substantial changes in fatty acid profiles and thereby in mycosubtilin isoforms, compared to the wild-type strain; and (iii) the presence of five novel mycosubtilin isoforms. The antifungal activities of the new mix were higher than or equal to those of purified isoforms. [less ▲]

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See detailInfluence of steam explosion on physico-chemical properties and hydrolysis rate of pure cellulose fibers
Jacquet, Nicolas ULg; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

in Bioresource Technology (2012), 121(221-227),

The aim of the present study is to compare the effect of different steam explosion treatments on physicochemical properties and hydrolysis rate of a pure bleached cellulose. The results showed that ... [more ▼]

The aim of the present study is to compare the effect of different steam explosion treatments on physicochemical properties and hydrolysis rate of a pure bleached cellulose. The results showed that moderate steam explosion treatments (severity factor below 5.2) did not improve the enzymatic hydrolysis rate of the cellulose fibers. The characterization of the obtained samples showed an increase of the cellulose accessibility coupled with an increase of the overall crystallinity of the substrate. In these conditions, the higher accessibility is counterbalanced by the increased crystallinity. Indeed, a greater proportion of the substrat is accessible by only a fraction of the enzymatic complex (exo-glucanases) activities. When the severity factor reached 5.2, a decrease of the cellulose enzymatic hydrolysis rate was observed. In this case, TGA analysis showed an increase of the char level at the end of the pyrolysis which traduced an important thermal degradation of the samples. The thermal degradation of cellulose lead to an important change in substrate composition, which induced a decrease of the cellulose ratio available for hydrolysis and caused a decrease of the hydrolysis yields. [less ▲]

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See detailStudy of the interactive effect of temperature and pH on exopolysaccharide 3 production by Enterobacter A47 using multivariate statistical analysis
Torres, Cristiana A.V.; Antunes, Silvia; Ricardo, Ana Rita et al

in Bioresource Technology (2012), 119

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See detailImpact of energy supply and oxygen transfer on selective lipopeptide production by Bacillus subtilis BBG21.
Fahim, Sameh; Dimitrov, Krasimir; Gancel, Frederique et al

in Bioresource technology (2012), 126

The influence of power dissipation and volumetric oxygen transfer coefficient k(L)a on Bacillus subtilis productivity of lipopeptides surfactin and fengycin was studied in shake flasks in view of scaling ... [more ▼]

The influence of power dissipation and volumetric oxygen transfer coefficient k(L)a on Bacillus subtilis productivity of lipopeptides surfactin and fengycin was studied in shake flasks in view of scaling-up of this fermentation process. The experiments performed with different flask sizes, relative filling volumes, and shaking frequencies confirmed clearly that lipopeptide production changed in function of power dissipation, via interfacial gas-liquid contact surface and oxygen supply. It was demonstrated that k(L)a is the key parameter controlling the productivity and the selectivity of the bioreaction. Varying the oxygen transfer conditions, the synthesis could be oriented to mixed production or to surfactin mono-production. The fraction of surfactin towards total lipopeptides produced and the maximal surfactin production both increased with k(L)a increase (surfactin concentration about 2 g L(-1) at k(L)a=0.04-0.08 s(-1)), while the maximal fengycin production (fengycin concentration about 0.3 g L(-1)) was obtained at moderate oxygen supply (k(L)a=0.01 s(-1)). [less ▲]

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See detailComparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria
Hiligsmann, Serge ULg; Masset, Julien ULg; Hamilton, Christopher ULg et al

in Bioresource Technology (2011), 102

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO2 and another gaseous compound such as hydrogen or ... [more ▼]

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO2 and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose. The experimental results compared the hydrogen production yield of 19 different pure strains and sludges : facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1,36 mol H2/mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains. [less ▲]

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See detailEffective Production of the Biodiesel Additive STBE by a Continuous Flow Process
Monbaliu, Jean-Christophe ULg; Winter, Marc; Chevalier, Bérengère et al

in Bioresource Technology (2011), 102

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See detailIsolation of bioactive peptides from tryptone that modulate lipase production in Yarrowia lipolytica
Turki, Souassen; Ben Kraeim, Ines; Weekers, F. et al

in Bioresource Technology (2009), 100

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See detailDietary fibre components and pectin chemical features of peels during ripening in banana and plantain varieties.
Happi Emaga, Thomas ULg; Robert, Christelle; Ronkart, Sébastien ULg et al

in Bioresource Technology (2008), 99(10), 4346-4354

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