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See detailSynoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation
Schneider, Nicole ULg; Mouithys-Mickalad, Ange ULg; Lejeune, Jean-Philippe ULg et al

in Biochemical & Biophysical Research Communications (2005), 334(2), 669-673

By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes ... [more ▼]

By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10(7)cells; O(2) consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n=3 for each cell line). Both types of synoviocytes showed a high O(2) consumption, which was slowered after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O(2) consumption, unchanged by anoxia, and no free radical production. These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis. [less ▲]

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See detailChemical structure of the cell wall of Mycobacterium smegmatis. I. Isolation and partial characterization of the peptidoglycan
Petit, J. F.; Adam, A.; Wietzerbin-Falszpan, J. et al

in Biochemical & Biophysical Research Communications (1969), 35(4), 478-485

Cell walls of were prepared by French press disruption and treatment with trypsin and chymotrypsin. Free lipids were extracted by organic solvents. Part of the bound lipids were solubilized by an alkaline ... [more ▼]

Cell walls of were prepared by French press disruption and treatment with trypsin and chymotrypsin. Free lipids were extracted by organic solvents. Part of the bound lipids were solubilized by an alkaline treatment and most of the remaining lipids and neutral sugars by mild acid hydrolysis, leaving an insoluble residue which has the composition of a peptidoglycan. This residue was solubilized by AL1 enzyme. It was possible to isolate from the lysate a tripeptide Ala-Glu-DAP, three tetrapeptides Ala-Glu-DAP-Ala and also a tetrasaccharide and a disaccharide which give equimolar amounts of glucosamine and muramic acid after acid hydrolysis. The disaccharide differs from the typical [beta]-1,4-N-acetylglucosaminyl-N-acetylmuramic acid by its Rf values in various solvents. [less ▲]

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See detailOn the Linkage between Teichoic Acid and the Glycopeptide in the Cell Wall of Staphylococcus Aureus
Strominger, Jack L.; Ghuysen, Jean-Marie ULg

in Biochemical & Biophysical Research Communications (1963), 12(5), 418-424

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