References of "Arthritis and Rheumatism"
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See detailGene expression pattern of synovial cells from inflammatory and normal areas of osteoarthritis synovial membrane.
Lambert, Cécile ULg; Dubuc, Jean-Emile; Montell, Eulalia et al

in Arthritis and Rheumatism (2014), sous presse

Objective: The aim of this study was to compare the gene expression pattern of synovial cells from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same ... [more ▼]

Objective: The aim of this study was to compare the gene expression pattern of synovial cells from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same osteoarthritis (OA) patient. Methods: Synovial tissues were obtained from 12 knee OA patients at the time of total knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria and sorted as N/R and I. Biopsies were cultured separately for 7 days. Microarray gene expression profiling between N/R and I areas was performed. Western blot and immunohistochemistry confirmed the identified genes that were differentially expressed. Results: 896 differentially expressed genes between N/R and I zones were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory network, TREM1 and S100A9 were strongly up-regulated. MMP-3 and -9, cathepsin H and S were significantly up-regulated in the cartilage catabolism pathway, whereas the most up-regulated anabolism enzyme was HAS1. Wnt-5A and LRP5 were up-regulated whereas FZD2 and DKK3 were down-regulated in the Wnt signaling. Finally, STC1, a protein involved in angiogenesis was identified as the most up-regulated gene in I zones compared to N/R zones. Conclusion: This study is the first to identify different expression pattern between two areas of the synovial membrane in the same patient. These differences concern several key pathways involved in OA pathogenesis. This analysis also provides information regarding new genes and proteins as potential targets for the future therapeutic. (c) 2013 American College of Rheumatology. [less ▲]

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See detailOleuropein or rutin consumption decreases the spontaneous development of OA in Hartley guinea pig
Sanchez, Christelle ULg; Horcajada, Marie-Noëlle; Membrez, Fanny et al

in Arthritis and Rheumatism (2013), 65

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See detailSirtuin 1 enzymatic activity is required for cartilage homeostasis in vivo in a mouse model.
Gabay, Odile; Sanchez, Christelle ULg; Dvir-Ginzberg, Mona et al

in Arthritis and Rheumatism (2013), 65(1), 159-66

OBJECTIVE: We and others previously demonstrated that sirtuin 1 (SIRT-1) regulates apoptosis and cartilage-specific gene expression in human chondrocytes and mouse models. This study was undertaken to ... [more ▼]

OBJECTIVE: We and others previously demonstrated that sirtuin 1 (SIRT-1) regulates apoptosis and cartilage-specific gene expression in human chondrocytes and mouse models. This study was undertaken to determine if SIRT-1 enzymatic activity plays a protective role in cartilage homeostasis in vivo, by investigating mice with SIRT-1 mutations to characterize their cartilage. METHODS: Articular cartilage was harvested from the paws and knees of 5- and 6-month-old wild-type (WT) mice and mice homozygous for SIRT-1(tm2.1Mcby) (SIRT-1(y/y) ), an allele carrying a point mutation that encodes a SIRT-1 protein with no enzymatic activity (y/y mice). Mice ages 2 days old and 6-7 days old were also examined. Mouse joint cartilage was processed for histologic examination or biochemical analyses of chondrocyte cultures. RESULTS: We found that articular cartilage tissue sections from y/y mice of up to 6 months of age contained reduced levels of type II collagen, aggrecan, and glycosaminoglycan compared to sections from WT mice. In contrast, protein levels of matrix metalloproteinase 8 (MMP-8), MMP-9, and MMP-13 were elevated in the cartilage of y/y mice. In addition, chondrocyte apoptosis was elevated in SIRT-1 mutant mice as compared to their WT littermates. Consistent with these observations, protein tyrosine phosphatase 1b was elevated in the y/y mice. CONCLUSION: Our in vivo findings in this animal model demonstrate that mice with defective SIRT-1 also have defective cartilage, with elevated rates of cartilage degradation with age. Hence, normal cartilage homeostasis requires enzymatically active SIRT-1 protein. [less ▲]

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See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Arthritis and Rheumatism (2012, October), 64(10 (Suppl)), 760

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See detailFibulin 3 peptides Fib3-1 and Fib3-2 are potential biomarkers of osteoarthritis.
Henrotin, Yves ULg; Gharbi, Myriam; Mazzucchelli, Gabriel ULg et al

in Arthritis and Rheumatism (2012), 64(7), 2260-7

OBJECTIVE: This study was undertaken to identify new biomarkers of osteoarthritis (OA) by proteomics analysis and to develop specific immunoassays to detect and quantify them. METHODS: Proteomics analysis ... [more ▼]

OBJECTIVE: This study was undertaken to identify new biomarkers of osteoarthritis (OA) by proteomics analysis and to develop specific immunoassays to detect and quantify them. METHODS: Proteomics analysis was performed in urine samples from 10 women (mean+/-SD age 76.0+/-5.0 years) undergoing knee replacement surgery due to severe OA and 5 healthy women (mean+/-SD age 25.6+/-2.6 years). Protein content was analyzed by 2-dimensional differential gel electrophoresis. Protein spots that exhibited an OA:control abundance ratio of >/=1.5 were identified by mass spectrometry. Specific enzyme-linked immunosorbent assays were developed and validated in serum obtained from 236 healthy subjects ages 20-64 years and from 76 patients with severe radiologic knee OA (mean+/-SD age 68.8+/-11.9 years). Immunohistochemical analysis was performed on articular cartilage from tibial plateaus. RESULTS: Thirteen proteins within spots that were significantly modified between groups were identified. Two peptides of fibulin 3, named Fib3-1 and Fib3-2, were of particular interest. Two antisera directed against these peptides were used to develop immunoassays. Compared with age-matched healthy subjects, median levels of serum Fib3-1 and Fib3-2 were elevated in OA patients (54.6 pM versus 85.1 pM [P<0.0001] and 144.4 pM versus 191.4 pM [P<0.0001], respectively). Using area under the receiver operating characteristic curve analysis, we demonstrated that Fib3-1 and Fib3-2 levels discriminate between OA and normal populations. Immunostaining revealed the presence of Fib3-1 and Fib3-2 in chondrocytes and in the extracellular matrix of the superficial layer of the fibrillated cartilage. CONCLUSION: Our findings indicate that Fib3-1 and Fib3-2 are potential biochemical markers for the diagnosis of OA. [less ▲]

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See detailHeterogeneous nuclear ribonucleoproteins as targets of autoantibodies in systemic rheumatic diseases.
Op De Beeck, Katrijn; Maes, Liesbeth; Van den Bergh, Karolien et al

in Arthritis and Rheumatism (2012), 64(1), 213-221

BACKGROUND: Heterogeneous nuclear ribonucleoproteins (hnRNP) are abundant nucleoplasmic pre-mRNA-binding proteins. The aim of this study was to unravel the mosaic of autoantibodies to hnRNP's in systemic ... [more ▼]

BACKGROUND: Heterogeneous nuclear ribonucleoproteins (hnRNP) are abundant nucleoplasmic pre-mRNA-binding proteins. The aim of this study was to unravel the mosaic of autoantibodies to hnRNP's in systemic rheumatic diseases. METHODS: Recombinant human hnRNP A1, B1, C1, E1, F, Gi, H1, I, K, and P2 were prepared. Antibodies to these antigens were determined by Western blotting and ELISA (for hnRNP B1, E1, F, and H1) in controls (chronic fatigue syndrome) and in patients with various connective tissue disorders. RESULTS: Western blotting analysis on 106 controls and 298 patients with a connective tissue disorder revealed that antibodies to all tested hnRNP antigens, except hnRNP Gi, were significantly more prevalent in Sjogren's syndrome (SS) than in controls. The highest reactivity was found for hnRNP B1, E1, F, and H1 (reactivity in >45% of patients with SS and in 2.8% of controls). Reactivity to hnRNP B1, E1, F, and H1 was also evaluated by ELISA in controls and in 228 patients with a connective tissue disease. Reactivity to at least 2 of the 4 tested antigens was found in 1.1% of controls, 16% of patients with systemic lupus erythematosus (SLE), and 18% of patients with SS. Reactivity to at least 3 of the 4 antigens was found in none of the controls, in 3.2% of patients with SLE, and in 15% of patients with SS. CONCLUSIONS: several hnRNP's are target antigens in SS. The combined presence of antibodies to several hnRNP's was strongly associated with connective tissue disease in general and SS in particular. [less ▲]

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See detailCorrection of vitamin D insufficiency with the fixed daily combination strontium ranelate 2 g/vitamin D3 1000 IU over 12 months
Rizzoli, R; Dawson-Hughes, B; Kaufman, JM et al

in Arthritis and Rheumatism (2012), 64(S10), 835

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See detailClinically meaningful effect of strontium ranelate on knee osteoarthritis symptoms
Bruyère, Olivier ULg; Bellamy, N; Brown, J et al

in Arthritis and Rheumatism (2012), 64(S10), 110

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See detailStrontium ranelate in knee osteoarthritis trial (SEKOIA) : a structural and symptomatic efficacy
Reginster, Jean-Yves ULg; Chapurlat, R; Christiansen, C et al

in Arthritis and Rheumatism (2012), 64(S10), 681

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See detailRegulation of subchondral bone osteoblast metabolism by cyclic compression.
Sanchez, Christelle ULg; Pesesse, Laurence ULg; Gabay, Odile et al

in Arthritis and Rheumatism (2012), 64(4), 1193-203

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure ... [more ▼]

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure applied to the articulation was responsible for these metabolic changes. This study was undertaken to evaluate the effects of cyclic compression on osteoblasts from OA subchondral bone. METHODS: Osteoblasts were isolated from sclerotic and nonsclerotic areas of human OA subchondral bone. After 28 days, the osteoblasts were surrounded by an abundant extracellular matrix and formed a resistant membrane, which was submitted to cyclic compression (1 MPa at 1 Hz) for 4 hours. Gene expression was evaluated by reverse transcription-polymerase chain reaction. Protein production in culture supernatants was quantified by enzyme-linked immunosorbent assay or visualized by immunohistochemistry. RESULTS: Compression increased the expression of genes coding for interleukin-6 (IL-6), cyclooxygenase 2, RANKL, fibroblast growth factor 2, IL-8, matrix metalloproteinase 3 (MMP-3), MMP-9, and MMP-13 but reduced the expression of osteoprotegerin in osteoblasts in both sclerotic and nonsclerotic areas. Colalpha1(I) and MMP-2 were not significantly affected by mechanical stimuli. Nonsclerotic osteoblasts were significantly more sensitive to compression than sclerotic ones, but after compression, differences in messenger RNA levels between nonsclerotic and sclerotic osteoblasts were largely reduced or even abolished. Under basal conditions, sclerotic osteoblasts expressed similar levels of alpha5, alphav, beta1, and beta3 integrins and CD44 as nonsclerotic osteoblasts but 30% less connexin 43, an important mechanoreceptor. CONCLUSION: Genes involved in subchondral bone sclerosis are mechanosensitive. After compression, nonsclerotic and sclerotic osteoblasts expressed a similar phenotype, suggesting that compression could be responsible for the phenotype changes in OA subchondral osteoblasts. [less ▲]

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See detailRelationship between changes in bone mineral density and incidence of fracture with 6 years of Denosumab treatment
Bolognese, MA; Miller, PD; Reginster, Jean-Yves ULg et al

in Arthritis and Rheumatism (2012), 64(S10), 847

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See detailEffects of Strontium ranelate on knee osteoarthritis pain : a responder analysis
Reginster, Jean-Yves ULg; Chapurlat, R; Bellamy, N et al

in Arthritis and Rheumatism (2012), 64(S10), 110

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See detailSIRT1-deficient mice exhibit an altered cartilage phenotype
Gabay, Odile; Sanchez, Christelle ULg; Dvir-Ginzberg, Mona et al

in Arthritis and Rheumatism (2011), 63(10), 702

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See detailExtended safety observations from denosumab administration in postmenopausal women from FREEDOM and FREEDOM extension trials
Brown, J. P.; Bone, H. G.; Chapurlat, R. et al

in Arthritis and Rheumatism (2011), 63(S10), 431-432

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See detailNew alginate-chitosan hydrogel beads with anti-inflammatory and anabolic effects on human chondrocytes
Oprenyeszk, Frédéric ULg; Sanchez, Christelle ULg; Dubuc, Jean-Emile et al

in Arthritis and Rheumatism (2011), 63(10), 697

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See detailMaintenance of antifracture efficacy over 10 years with strontium ranelate in postmenopausal osteoporosis
Reginster, Jean-Yves ULg; Kaufman, J. M.; Devogelaer, J. P. et al

in Arthritis and Rheumatism (2011), 63(S10), 436

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See detailEffectiveness of zoledronic acid in the prevention and treatment of glucocorticoid-induced osteoporosis in men and premenopausal women
Saag, k; Roux, C.; Devogelaer, J. P. et al

in Arthritis and Rheumatism (2010, October), 62(10), 902-903

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See detailLong-term denosumab treatment of postmenopausal women with osteoporosis: results from the first year extension study of the FREEDOM trial
Chapurlat, R.; Papapoulos, Socrates; Bone, Henry G et al

in Arthritis and Rheumatism (2010, October), 62(10), 903

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