References of "Anticancer Research"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailDetection and quantification of human papillomavirus in benign and malignant parotid lesions.
Descamps, Geraldine; Duray, Anaelle; Rodriguez, Alexandra et al

in Anticancer Research (2012), 32(9), 3929-32

Background/Aim: Human papillomavirus (HPV) is implicated in head and neck squamous cell carcinomas. However, the causal role of HPV in carcinomas of the parotid gland remains uncertain and less documented ... [more ▼]

Background/Aim: Human papillomavirus (HPV) is implicated in head and neck squamous cell carcinomas. However, the causal role of HPV in carcinomas of the parotid gland remains uncertain and less documented. This study aimed to determine the potential implication of HPV in the development of benign and malignant lesions of the parotid gland. MATERIALS AND METHODS: Paraffin-embedded biopsies were obtained from 40 patients with benign parotid gland tumors and from 39 patients with parotid gland carcinomas. The 79 samples were evaluated for the presence of HPV DNA using both GP5+/GP6+ consensus Polymerase Chain Reaction (PCR) and type-specific E6/E7 PCR to detect 18 HPV types. RESULTS: Our results showed a low prevalence of HPV, with only three HPV-positive cases among the 40 benign tumors and one infected carcinoma in the malignant population. CONCLUSION: No association between the presence of HPV DNA and the development of parotid gland tumors was found in our study. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailOverexpression of CD9 in human breast cancer cells promotes the development of bone metastases.
Kischel, Philippe; Bellahcene, Akeila ULg; Deux, Blandine et al

in Anticancer Research (2012), 32(12), 5211-20

BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental ... [more ▼]

BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental MDA-MB-231 breast cancer cell line. Here, we investigated the putative relationship between CD9 expression and the osteotropic phenotype. MATERIALS AND METHODS: Overexpression of CD9 was analyzed by immunoblotting in different cell lines. Immunohistochemistry was used to assess CD9 expression in primary tumors and metastatic lesions. In vivo experiments were conducted in mice using a monoclonal antibody against CD9. RESULTS: CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. Finally, in vivo experiments showed that an antibody against CD9 delays homing of B02 cells in bone marrow, slowing down bone destruction. CONCLUSION: Our study reveals a potential implication of CD9 in the formation of bony metastases from breast cancer cells. [less ▲]

Detailed reference viewed: 17 (3 ULg)
Full Text
Peer Reviewed
See detailFirst study of oral artenimol-R in advanced cervical cancer: clinical benefit, tolerability and tumor markers
Jansen, Frans Herwig; Adoubi, Innocent; Kouassi, Comoe J.C. et al

in Anticancer Research (2011), 31(12), 4417-4422

Background/Aim: Artenimol-R is cytotoxic in transformed cervical cells and safety in humans is yet to be established. The present study investigates the clinical benefits, safety and the tumor marker ... [more ▼]

Background/Aim: Artenimol-R is cytotoxic in transformed cervical cells and safety in humans is yet to be established. The present study investigates the clinical benefits, safety and the tumor marker effect of orally administered Artenimol-R in patients with advanced cervix carcinoma. Patients and Methods: Ten patients were treated with Artenimol-R for 28 days. Clinical symptoms, vaginal discharge and pain were followed-up. Adverse events were recorded. Biopsy samples were analyzed by immunohistochemistry for the expression of relevant tumor markers. Results: Artenimol-R treatment induced clinical remission with a median time for the disappearance of the symptoms being 7 days. No adverse events of grade 3 or 4 occurred. The expression of p53, Epidermal growth factor receptor (EGFR), and antigen Ki-67 as a cellular marker of proliferation, as well as the number of blood vessels stained by the CD31 antibody decreased, whereas the expression of transferrin receptor protein 1 (CD71) increased. Conclusion: The current pilot study provides evidence on the improvement of the clinical symptoms and the good tolerability of Artenimol-R in patients with advanced carcinoma of the cervix uteri. A survival trial with Artenimol- R in advanced patients is warranted. [less ▲]

Detailed reference viewed: 40 (2 ULg)
Full Text
See detailLNCaP prostate cancer imaging with biologically functionalized gold nanoparticles in 2D and 3D cell culture
Schol, Daureen ULg; Fleron, Maximilien ULg; Greisch, Jean - François et al

in Anticancer Research (2008), 28

One of the main objectives of this project is to realize and validate a versatile lab system composed of functionalized nanoparticles for diagnosis of different superficial and accessible cancers, e.g ... [more ▼]

One of the main objectives of this project is to realize and validate a versatile lab system composed of functionalized nanoparticles for diagnosis of different superficial and accessible cancers, e.g. prostate cancer. Gold nanorods have been synthesized and functionalized with antibodies targeting specific antigens on cancer cell lines. [less ▲]

Detailed reference viewed: 67 (21 ULg)
Full Text
Peer Reviewed
See detailProteomic analysis of telomerase inhibition by telomere specific ligands
Mazzucchelli, Gabriel ULg; Gabelica, Valérie ULg; Smargiasso, Nicolas ULg et al

in Anticancer Research (2008), 28(5c), 3257-3258

Telomeres consist of protein complexes and repeated ‘TTAGGG’ double strand DNA sequences ended by a 3’ single strand DNA of the same sequence. Progressive telomere shortening is observed in vitro upon ... [more ▼]

Telomeres consist of protein complexes and repeated ‘TTAGGG’ double strand DNA sequences ended by a 3’ single strand DNA of the same sequence. Progressive telomere shortening is observed in vitro upon cell divisions and with ageing in vivo. At a critical telomere length, shortened telomeres trigger a permanent growth arrest known as replicative senescence. Telomerase is an RNA-dependent DNA polymerase that extends telomeres by adding ‘TTAGGG’ repeats. It consists of a functional RNA component (hTR) which serves as template and a catalytic protein (hTERT) with reverse transcriptase activity. The expression of hTERT alone is sufficient for the immortalisation of cells. Telomerase is highly expressed in tumor cells but at very low level in most somatic cells. These observations make the telomerase an attractive target for anticancer strategies. One of these strategies relies on the use of drug candidates able to stabilize the particular telomere G-quadruplex DNA structures. The stabilization of these structures makes the telomere inaccessible for telomerase and thus inhibits telomerase activity. The effect of the hTERT transfection was first studied on the proteome of human WI38 fibroblast cells (1). Then, the proteome alteration response of hTERT transfected WI38 cells induced by the treatment of two G-quadruplexes ligands, telomestatin and TMPyP4, was analyzed. Both compounds can inhibit telomerase but have different selectivity for the different G-quadruplexes structures. Proteome analysis of the treated cells reveals that TMPyP4 induces much more protein expression alterations than telomestatin probably due to its poor selectivity. TMPyP4 induces especially a drastic down expression of the hnRNPs, a modulation of the proteasome pathway, an apparent decrease of the translation and an over expression of several molecular chaperones. Telomestatin induces in particular an over expression of the protein BCL2A1 which is involved in drug resistance of cancer cells and a probable increase of the translation. Both treatments have a common effect particularly on the molecular chaperone CCT (down expression), HSP90 alpha (over expression) and hnRNP D (down expression). The protein HSP90 alpha is also over expressed in hTERT transfected cells compared to parental cells. This protein is already a promising anticancer target protein due to its central role in oncogenesis and in telomerase activity regulation. 1 Mazzucchelli et al: Proteome Science 6: 12, 2008. [less ▲]

Detailed reference viewed: 118 (25 ULg)
Full Text
Peer Reviewed
See detailApoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells
Gerkens, Pascal; Dobson, Rowan ULg; tabatadze, Nino et al

in Anticancer Research (2007), 27

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells ... [more ▼]

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells. Materials and Methods: the end-point metabolic (WST1) and lactate dehydrogenase (LDH) assays were used. Cell cycle analysis and apoptosis were measured by flow cytometry, DNA laddering and caspase 3 analyses. Results: the HL-60 cell line was more sensitive to Hcol-A1 and Gig-D (IC50 3-5 ÌM) than to Gig-E and Hcol-A (IC50 8-13 ÌM; WST1 assay). This was related to LDH release. The induction of apoptosis could be detected without caspase 3 activation after 24 h of treatment. DNA fragmentation could be detected only with Gig-D. With Hcol- A1 and Gig-D, an accumulation of cells in the S-phase and an increase of cells in sub-G1 peak were observed. By the annexinV-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (AAD) assay, the majority of cells were in late apoptosis with Gig-D, and in necrosis with Hcol-A1. Conclusion: Hcol-A1 is more cytotoxic than Gig-D, followed by Gig-E and finally Hcol-A. This is related to a membrane permeabilization effect, leading to cytolysis [less ▲]

Detailed reference viewed: 54 (11 ULg)
Full Text
Peer Reviewed
See detailInduction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane Diterpene
Block, Sébastien; Gerkens, Pascal; Peulen, Olivier ULg et al

in Anticancer Research (2005), 25(1A), 363-368

Background: Trachylobane diterpenes are secondary metabolites quite rare in nature and their bioactivities are poorly known. Recently we have described the cytotoxic activity of ent- trachyloban-3‚-ol ... [more ▼]

Background: Trachylobane diterpenes are secondary metabolites quite rare in nature and their bioactivities are poorly known. Recently we have described the cytotoxic activity of ent- trachyloban-3‚-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine. Materials and Methods: Cell viability on several cell lines, cell morphology, DNA laddering, annexin V and caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis. Results: ent-trachyloban-3‚-ol exerts a dose-dependent cytotoxic effect and which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 ÌM after 24-h treatment. Conclusion: We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner. [less ▲]

Detailed reference viewed: 17 (5 ULg)
Full Text
Peer Reviewed
See detailSequential administration of epirubicin and paclitaxel for advanced breast cancer. A phase I randomised trial.
Focan, C.; Graas, M. P.; Beauduin, M. et al

in Anticancer Research (2005), 25(2B), 1211-7

Forty-six previously untreated patients with advanced breast cancer were eligible for the present randomised phase I study. It aimed to evaluate the toxicity and activity of a therapeutic sequence with ... [more ▼]

Forty-six previously untreated patients with advanced breast cancer were eligible for the present randomised phase I study. It aimed to evaluate the toxicity and activity of a therapeutic sequence with epirubicin on day 1 followed by paclitaxel on day 2 (sequence A) or the reverse sequence, ie., paclitaxel on day 1 followed by epirubicin on day 2 (sequence B). The starting doses of epirubicin and paclitaxel, administered either according to sequence A or B, (level 1 cohort) were 90 mg/m2 and 175 mg/m2, respectively. Per cohort of 3 patients, the dose of paclitaxel was increased by 25 mg/m2 (levels 2 and 4) and of epirubicin by 10 mg/m2 (levels 3 and 5). Treatment was repeated with 3-week intervals. The maximal tolerated dose (MTD) was achieved at level 1 in sequence B (paclitaxel first) and level 3 (epirubicin 100 mg/m2 followed by paclitaxel 200 mg m2) in sequence A. Dose limiting toxicity (DLT) was neutropenia (+/- febrile) in both sequences. Cardiac events occurred in 28% of the patients; significant decrease in left ventricular ejection function (LVEF) was observed in 8/33 and in 2/13 patients in sequence A and B, respectively. This was associated with 5 and 1 cardiac heart failure (CHF), respectively. In 43 evaluable patients, 10 CR and 25 PR were observed (overall response rate 81%). In the 20 patients with locally advanced disease (LABC), the respective numbers were 7 CR and 11 PR; in the 23 metastatic (MBC) patients, 3 CR and 14 PR were recorded. The median survival of the both groups was not reached at 33 + months. In conclusion , the combination of epirubicin and paclitaxel has significant activity in breast cancer. The recommended sequence of both drugs in combination therapy, mainly to avoid neutropenia, is epirubicin day 1 followed by paclitaxel on day 2. Cardiac toxicity remains problematic in either sequence of administration. [less ▲]

Detailed reference viewed: 26 (1 ULg)
Peer Reviewed
See detailThe plant alkaloid usambarensine intercalates into DNA and induces apoptosis in human HL60 leukemia cells
Dassonneville, L.; Wattez, N.; Mahieu, C. et al

in Anticancer Research (1999), 19(6B), 5245-5250

Detailed reference viewed: 30 (3 ULg)
Peer Reviewed
See detailApoptosis During the Development of Radiogenic Thymic Lymphomas: Effects of Treatments Inhibiting Lymphoma Development
Humblet, Chantal ULg; Denis, Ghislaine; Greimers, Roland ULg et al

in Anticancer Research (1998), 18(5A, Sep-Oct), 3469-74

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an ... [more ▼]

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an abnormal thymic microenvironment. A bone marrow graft or repeated cytokine injections prevent lymphoma development. We think that these treatments restore altered mechanisms controlling apoptosis. MATERIALS AND METHODS: Apoptosis was analyzed by flow cytometry in thymocytes from different groups of mice (control, preleukemic, prevented mice). RESULTS: The apoptotic rates did not change in freshly isolated thymocytes from different experimental groups. However, after culture, the level of apoptosis increased in preleukemic thymuses; and returned to normal value in cultured thymocytes from irradiated mice after lymphoma preventing treatments. Furthermore, thymic microenvironmental factors can control thymocyte apoptosis. CONCLUSION: We propose that after leukemogenic irradiation, there is an increase of cells with an activated suicide program, but that alterations of thymic environmental factors rescue them from apoptosis, allowing their further neoplastic transformation. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Peer Reviewed
See detailP53, Bax and Bcl-2 in Vivo Expression in the Murine Thymus after Apoptogenic Treatments
Denis, Ghislaine; Humblet, Chantal ULg; Verlaet, Myriam ULg et al

in Anticancer Research (1998), 18(5, Sep-Oct), 3315-21

BACKGROUND: Neoplasia can results from a lack of cell elimination by apoptosis. In order to determine if mechanisms controlling apoptosis are disturbed during neoplastic transformation in a model of ... [more ▼]

BACKGROUND: Neoplasia can results from a lack of cell elimination by apoptosis. In order to determine if mechanisms controlling apoptosis are disturbed during neoplastic transformation in a model of murine radio-induced thymic lymphomas, we have assessed the kinetics of p53, Bax and Bcl-2 in situ expression after induction of thymic apoptosis by irradiation or glucocorticoids at first in normal mice. MATERIALS AND METHODS: TUNEL method was used for in situ detection of apoptosis and protein expression was determined by indirect immunohistochemistry. RESULTS: After hydrocortisone injection, levels of p53 and Bax, but not Bcl-2, expression were raised. A whole body sublethal irradiation led to an increase of p53 and Bcl-2, but not Bax, expression. CONCLUSIONS: This is the first in vivo report of in situ protein expression in the thymus after apoptogenic treatments of mice. The results suggest that Bax could be involved in glucocorticoid-mediated apoptosis. The increased levels of Bcl-2 expression are discussed. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailCytotoxic interactions of 5-fluorouracil and nucleoside analogues in vitro
Li, Y-X; COUCKE, Philippe ULg; Paschoud, N et al

in Anticancer Research (1997), 17(1A), 21-27

The cytotoxic interaction of combined 5-fluorouracil (5-FU) with different nucleoside analogues was investigated in vitro on a colon (WiDr) and a breast (MCF-7) cancer cell line. Azidothymidine (AZT), 3 ... [more ▼]

The cytotoxic interaction of combined 5-fluorouracil (5-FU) with different nucleoside analogues was investigated in vitro on a colon (WiDr) and a breast (MCF-7) cancer cell line. Azidothymidine (AZT), 3'-deoxy-2', 3'-didehydrothymidine (D4T), 5-iododeoxyuridine (IdUrd) and 2',3'-dideoxycytidine (DDC) were tested at different concentrations (5-600 microM) as modulators of 5-FU. The experimental endpoints were cellular viability and cell cycle distribution. The combination of 5-FU and AZT or D4T yielded supra-additive cytotoxic effects in both cell lines at all concentrations. On WiDr, IdUrd at high concentrations of 50 and 100 microM showed a supra-additive effect whereas at low concentrations (5, 10 and 20 microM) the effect was antagonistic. 5-FU combined with IdUrd produced a synergistic effect on MCF-7 cells at all concentrations. DDC antagonised the toxic effect of 5-FU on the WiDr cell line. In WiDr cells, a significant increase in the overall S-phase was observed 48 and 72 hours after exposure to D4T, AZT and DDC at the low concentration of 10 microM. On the contrary, this accumulation in S-phase was not present in MCF-7 cells. The combined effect of 5-FU and nucleoside analogues in vitro is dependent on the type and concentration of nucleosides and the cell-line tested. AZT, D4T and IdUrd are more likely to be subjected to more intensive in vitro and in vivo research as far as modulation of 5-FU toxicity is concerned. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailIn Vitro Cytotoxic Activity of Two Potential Anticancer Drugs Isolated from Strychnos: Strychnopentamine and Usambarensine
Bonjean, K. A.; Gillet, Marie-Claire ULg; Quetin-Leclercq, J. et al

in Anticancer Research (1996), 16(3A, May-Jun), 1129-37

The cytotoxicity and the selective antiprotozoal activity of some Strychnos alkaloids, namely strychnopentamine (SP) and usambarensine (US) (7) led us to analyze and compare their effects with emetine (EM ... [more ▼]

The cytotoxicity and the selective antiprotozoal activity of some Strychnos alkaloids, namely strychnopentamine (SP) and usambarensine (US) (7) led us to analyze and compare their effects with emetine (EM) by using mouse B16 melanoma cells cultivated in vitro. We observed by cytological analysis and proliferation rate studies that these substances induce analogous cytotoxic effects in B16 cells, but at different concentrations i.e. formation of lamellar bodies in the cytoplasm, the which contain pre-melanosomes in the case of SP and US, vacuoles and blebs. At concentrations near their respective IC50, SP and US, but not EM, decreased colony formation. We showed by incorporation of labelled precursors that SP and US first inhibit RNA synthesis while EM initially acts on protein synthesis. These alkaloids increased melanin synthesis. Furthermore, only EM and SP caused hemolysis of sheep red blood corpuscles. This could explain why the rate of antiplasmodial activity is higher for SP and EM. [less ▲]

Detailed reference viewed: 9 (3 ULg)
Peer Reviewed
See detailHeterotransplantation of Primary and Established Human Tumour Cells in Nude Mice
Noël, Agnès ULg; Borcy, V.; Bracke, M. et al

in Anticancer Research (1995), 15(1, Jan-Feb), 1-7

Previous successful transplantations of human tumour cells into athymic nude mice have been described when cells were injected with a reconstituted basement membrane (matrigel). We have compared the ... [more ▼]

Previous successful transplantations of human tumour cells into athymic nude mice have been described when cells were injected with a reconstituted basement membrane (matrigel). We have compared the development and the histology of tumours following injection with matrigel or with culture medium of a panel of tumour cells exhibiting different degrees of tumorigenicity. Two cell lines (MCF7 and MCF7/6) required matrigel in order to form tumours. When inoculated with matrigel, all the other cell lines tested [MCF7 gpt, MCF7ras, MCF7(AZ), MCF7(AZ)TD5, MDA-MB 231, HT1080] showed increased tumour take and reduced latency period. Human primary tumours (melanoma, breast and colon cancers) were transplanted successfully into nude mice, in the presence of matrigel. Breast primary tumours or cell lines gave rise to poorly differentiated carcinomas. The other tumours presented histopathological patterns typical of differentiated human cancers. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailEvaluation of Matrix Metalloproteinases and Serine Proteases Activities in Three B16 Melanoma Cell Lines with Distinct Tumorigenic Potential
Baramova, E. N.; Coucke, P.; Leprince, Pierre ULg et al

in Anticancer Research (1994), 14(3A, May-Jun), 841-6

Mouse B16 melanoma cells (B16, parental line) and two derived clones either pigmented (B16P) or non pigmented (B16NP) were cultured as monolayers (2D) or on agar, as aggregates (3D). The productions of ... [more ▼]

Mouse B16 melanoma cells (B16, parental line) and two derived clones either pigmented (B16P) or non pigmented (B16NP) were cultured as monolayers (2D) or on agar, as aggregates (3D). The productions of gelatinases A and B (72 kDa and 92 kDa type IV collagenases) and their inhibitors (TIMP1 and TIMP2), plasminogen activators (PAs) and plasminogen activator inhibitors (PAI) were investigated. The B16 cell lines did not secrete any gelatinase, but they secreted TIMP2, tissue-type (t-PA), urokinase-type (u-PA) plasminogen activators and PAI-1 like activities. High levels of PAI activity were determined in conditioned media and cellular extracts of B16NP, which could account for the lower tumorigenic potential of these cells. In 3D cultures, the cellular extracts of the three cell lines contained essentially u-PA activity. This activity could contribute to the greater tumorigenic and invasive capacities of B16, B16P and B16NP when cultured in 3D. [less ▲]

Detailed reference viewed: 14 (3 ULg)
Peer Reviewed
See detailLaminin and 67 Kd Laminin Binding Protein in Mouse B16 Melanoma Cells and 3t3 Fibroblast Spheroids
Siwek, B. L.; Munaut, Carine ULg; Bonjean, K. A. et al

in Anticancer Research (1992), 12(6B, Nov-Dec), 2011-6

Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts ... [more ▼]

Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts. Their volume and proliferation or necrosis rate were evaluated. As measured by dot blot immunoassay, laminin was mainly produced by fibroblasts rather than by melanoma cells. High levels of laminin B1 chain mRNA were detected only in spheroids composed of 3T3 fibroblasts. The levels of 67 kD laminin binding protein mRNA were high in all cell populations studied here. [less ▲]

Detailed reference viewed: 30 (3 ULg)
Full Text
Peer Reviewed
See detailCytotoxic and mitogenic activities in culture media conditioned by mouse B16 melanoma cells and 3T3 fibroblasts
Siwek, Brigitte; Wauthy, Jacques ULg; Coucke, Paul et al

in Anticancer Research (1991), 11(2), 755-759

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They ... [more ▼]

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They are generally more active on B16 than on fibroblasts. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailCytological effects of culture media conditioned B16 melanoma cells and 3T3 fibroblasts
Coucke, Paul; Siwek, B.; Gillet, Marie-Claire ULg et al

in Anticancer Research (1991), 11(2), 801-804

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these ... [more ▼]

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these fractions revealed in them mitochondria swelling, blebs, broken membranes and dead cells. [less ▲]

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailEffects of FeSO4 on B16 melanoma cells differentiation and proliferation
Gillet, Marie-Claire ULg; Siwek, Brigitte; Pozzi, G. et al

in Anticancer Research (1990), 10(4), 1029-1033

The paper shows that FeS04 can increase or reduce B16 cell proliferation and melanogenesis. Vitamin C toxicity for B16 cells was reduced in the presence of FeSO4.

Detailed reference viewed: 18 (2 ULg)
Full Text
Peer Reviewed
See detailControl of B16 mealnoma cells differentiation and proliferation by CuSO4 and vitamin C
Gillet, Marie-Claire ULg; Siwek, Brigitte; Pozzi, G. et al

in Anticancer Research (1990), 10(2A), 391-405

The paper demonstrates that the toxicity of CuSO4 in B16 melanoma cells is increased in serum-free medium and in the presence of Vitamin C. Vitamin C toxicity for B16 cells was increased in the presence ... [more ▼]

The paper demonstrates that the toxicity of CuSO4 in B16 melanoma cells is increased in serum-free medium and in the presence of Vitamin C. Vitamin C toxicity for B16 cells was increased in the presence of CUS04. [less ▲]

Detailed reference viewed: 18 (3 ULg)