References of "Animal Reproduction Science"
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See detailReproduction in the endangered African wild dog: basic physiology, reproductive suppression and possible benefits of artificial insemination
Van Den Berghe, Femke ULg; Paris, Damien; Van Soom, Ann et al

in Animal Reproduction Science (2012), 133

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See detailPlasma urea nitrogen in relation to pregnancy rate in dairy sheep
Karen, Aly; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Animal reproduction science (2011), 124(1-2), 69-72

The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were ... [more ▼]

The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were used in the present study. Ewes were fed a diet containing 17.4% crude protein and were milked twice a day by the milking machine. The ewes were synchronized for estrus by insertion of intravaginal sponges containing 30mg flurogestone acetate for 14 days. At the time of sponge removal each ewe was administered eCG (600IU). All ewes were inseminated twice with fresh semen into the external os of the cervix at 48 and 56h after sponge removal. The day of insemination was considered as Day 0 for calculating the gestational period. Blood samples were collected from each ewe at Days 0, 18 for measurement of PUN concentrations and at Day 22 after AI for measurement of pregnancy-associated glycoprotein (PAG) by radioimmunoassay (RIA). Thirty-eight ewes (20.5%) were confirmed pregnant by PAG-RIA test at Day 22 and by ultrasonography at Day 80. The mean (+/-S.D.) concentration of PUN in all ewes at Day 0 was 12.7+/-4.6mmol/L. There were non-significant differences in the level of PUN between pregnant and non-pregnant ewes at Days 0 (12.2+/-4.2mmol/L vs. 12.8+/-4.7mmol/L, respectively) and 18 (9.6+/-2.9mmol/L vs. 10.4+/-4.0mmol/L, respectively) after AI. Mean PUN concentrations decreased significantly from Day 0 to Day 18 after AI in both pregnant and non-pregnant ewes. By using logistic regression analysis, there was no effect of PUN concentrations on the probability of pregnancy occurrence in the studied ewes (odds ratio: 0.97; 95% confidence interval: 0.9-1.05; P=0.45). In conclusion, there was no evidence of a relationship between PUN concentration and pregnancy rate for lactating AwassixMerino ewes in the present study because of low pregnancy rate observed. [less ▲]

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See detailIs neutrophil elastase associated with myeloperoxidase concentration and post-thawing parameters in equine frozen semen?
Ponthier, Jérôme ULg; de la Rebière de Pouyade, Geoffroy ULg; Desvals, Maud et al

in Animal Reproduction Science (2010), 121S

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no ... [more ▼]

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no effect on semen characteristics and to be not associated with on-sperm cells and freezability. [less ▲]

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See detailEquine frozen semen parameters in relation with total myeloperoxidase concentration
Ponthier, Jérôme ULg; Franck, Thierry ULg; Mottart, Evelyne et al

in Animal Reproduction Science (2008, September 15)

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See detailIdentification of multiple pregnancy-associated glycoproteins (PAGs) purified from the European bison (Eb; Bison bonasus L.) placentas.
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Animal Reproduction Science (2008), 112

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were ... [more ▼]

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were extracted from various cotyledonary tissues, precipitated, chromatographed (DEAE and VVA: Vicia villosa agglutinin), electrophoresed (1D- and 2D-PAGE), analysed by heterologous (cross-species) Western blotting and then micro-sequenced by Edman degradation. Finally, twelve selected VVA-purified isoforms (Ip 3.7-7.4) were entirely characterised. Nine identified NH(2)-terminal micro-sequences were found to be PAGs. On 45dpc, three identified forms were named: EbPAG(67AkDa) (RGSNLTHPLRNIGDLFYVGN), EbPAG(55BkDa) (RGSNLTHPL) and EbPAG(50CkDa) (SQISLRGSNLTI). On 60dpc, the next three forms were named: EbPAG(71DkDa) (RGSNLTIHPLRNIIDLFYVG), EbPAG(55EkDa) (RGSNLTHPLRNI) and EbPAG(50FkDa) (SQISLRGS). On 120dpc, three other forms were named: EbPAG(71GkDa) (RGSNLTHPLRNIRDLFYVG), EbPAG(60HkDa) (RGSNLTTHPLRNIKDLVVYM) and EbPAG(50IkDa) (SGSNLTTV). These EbPAG ((A-I)) sequences are unique, as they are not identical to any other PAGs purified previously in related species of the Bovidae family. However, the EbPAGs (A-I forms) have some sequence resemblance to internal sequences of various full-length polypeptide PAG precursors (in silico translated from cloned cDNAs) identified in domestic cattle. Three other novel native isoforms (J1, J2 and K): EbUPG(45kDa) J1 (SKDNYKNYIPLIVPFAT), EbUPG(45kDa) J2 (SKDNQKNYIPLIVPFAT) and EbUPG(76kDa) K (SPEFTV), were temporarily named 'unknown placental glycoproteins' (UPGs), due to their efficient VVA-purification (specific for glycoproteins only) and a lack of considerable consensus to previously sequenced placental glycoproteins in the Bovidae family. This is the first study identifying NH(2)-terminals of multiple/diverse EbPAGs and some EbUPGs purified from the synepitheliochorial cotyledonary placenta of the endangered Bison bonasus (Red list) [less ▲]

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See detailQTL and candidate genes for fecundity in sows
Buske, Bernd ULg; Sternstein, Ina; Brockmann, Gudrun

in Animal Reproduction Science (2006), 95(3), 167-183

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See detailChorionic mRNA expression and N-glycodiversity of pregnancy-associated glycoprotein family (PAG) of the European bison (Bison bonasus)
Szafranska, Bozena; Panasiewicz, G.; Dabrowski, M. et al

in Animal Reproduction Science (2005), 88(3-4), 225-243

Placental PAG mRNA expression and N-glycodiversity of multiple PAG proteins secreted in vitro by trophectoderm (chorion epithelium) of wild pecoran Bovidae taxons was not examined previously. The study on ... [more ▼]

Placental PAG mRNA expression and N-glycodiversity of multiple PAG proteins secreted in vitro by trophectoderm (chorion epithelium) of wild pecoran Bovidae taxons was not examined previously. The study on European bison (Eb) aimed: (1) to determine placental PAG mRNA expression by in situ hybridisation; (2) to identify a profile of pecoran PAG protein family secreted in vitro by cotyledonary (CT) explants; (3) to examine N-glycodiversity of the PAG proteins in this wild taxon. In addition, we compared (4) a profile and N-glycodiversity of the PAG protein family secreted in vitro by CT and interCT-trophectoderm (intCT-TRD) explants of domestic ruminants. Cotyledonary sections of the Eb were used for in situ hybridisation (ISH) with S-35-labelled probes produced with porcine PAG cDNA as templates. Various CT and intCT-TRD explants were long-term cultured in vitro. Chorionic proteins were isolated from media, ultra-filtrated ( > 10 kDa MWCO) and analysed by PAGE-Western blotting with various polyclonal anti-PAG sera. Protein samples with or without enzymatic deglycosylation were examined after different times of explant cultures. Released chorionic proteins were deglycosylated by N-glycanase F (PNGase F+) and compared to glycosylated forms (PNGase F-). This is the first paper demonstrating the PAG-like mRNA transcript expression (by ISH) and N-glycodiversity of immuno-reactive PAG-like proteins (produced in vitro by chorionic explants) of European bison. Various PAG proteins of Eb (EbPAG) were secreted by CT explants during long-term in vitro studies. Major similar to 78, similar to 67 and similar to 65 kDa EbPAG-like proteins were reduced by enzymatic deglycosylation (at least by 10 kDa). Considerably smaller amounts of similar to 45 kDa EbPAG-like proteins were also observed. In addition, we have found that various PAG proteins (30-73 kDa) were secreted by bovine CT explants, during long-term in vitro cultures. Corresponding amounts of PAG proteins, similar in M-r, were also secreted by intCT-TRD explants, whose tissues were not utilised for PAG protein extraction during other scientists' previous studies. It seems that the M-r-heterogeneity and N-glycodiversity of the PAG protein family can play very important role during feto-placental interactions in Bovidae species. [less ▲]

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See detailHormonal measurements in late pregnancy and parturition in dairy cows - possible tools to monitor foetal well being
Kornmatitsuk, B.; Veronesi, M. C.; Madej, A. et al

in Animal Reproduction Science (2002), 72(3-4), 153-164

Three dairy heifers (A, B and C) were induced to parturition with two prostaglandin (PG) F-2alpha injections on day 268 and 269 of pregnancy. Signs of approaching parturition were carefully observed. The ... [more ▼]

Three dairy heifers (A, B and C) were induced to parturition with two prostaglandin (PG) F-2alpha injections on day 268 and 269 of pregnancy. Signs of approaching parturition were carefully observed. The following parameters were registered: degrees of calving difficulty, date and time of parturition, calf's birth weight and calf's sex. Body temperature was measured and blood samples were taken every 3 h 3 days before the first PGF(2alpha) injection until 3 days after parturition. The plasma concentrations of the PGF2a metabolite, progesterone, cortisol, oestrone sulphate and pregnancy associated glycoproteins (PAGs) were analysed. Heifers A, B and C delivered 48, 51 and 57 h after the first PGF(2alpha) injection, respectively. Heifer A delivered without any signs of calving difficulty, whereas, the parturition was considered to be slight and moderate difficulty occurred in the delivery of heifers B and C, respectively. The calf of heifer C, without any abnormal gross-evidences, was stillborn. All animals had retained foetal membranes. A slight increase of the PGF(2alpha) metabolite at the time of parturition was found only in heifer C, whereas the levels dramatically increased in all animals 15-24 h after parturition. At the same time, progesterone levels decreased within 3 h after the first PGF(2alpha) injection (P < 0.05) and reached 0.8, 2.7 and 12.4 nmol/l at the time of parturition in heifers A, B and C, respectively. High release of cortisol at the time of parturition was seen in heifer C. Rising levels of oestrone sulphate around the time of parturition were recorded in all heifers, whereas, increasing levels of PAGs were recorded only in heifer A. In conclusion, the patterns of the PGF(2alpha) metabolite, cortisol, progesterone and PAGs were changed in the cases of calving difficulty and stillbirth after PGF(2alpha)-induction of parturition. However, the relationship between oestrone sulphate and PAGs and the status of foetal well being prior to parturition require further elucidation. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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