  Development of a performant method for glucocapparin determination in Boscia senegalensis Lam ex. Poir. : A study of the variability; ; et al in American Journal of Analytical Chemistry (2013), 4 This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X − 0.03 (R2 = 0.9998 ... [more ▼] This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X − 0.03 (R2 = 0.9998). Enzymatic hydrolysis of glucocapparin extracted from leaves and fruits of B. senegalensis, analyzed by SPME-GC-MS confirmed the presence of methylisothiocyanate as the main hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. senegalensis leaves and fruits collected in 4 localities in Senegal showed differences between organs according localities and periods of harvest. Glucocapparin content was very high in dry season particularly in January and the lowest rates were recorded during the rainy period between August and November. [less ▲] Detailed reference viewed: 19 (3 ULg) Fast-GC quantifacation of harmonine, the major defense alkaloid of the multicolored Asian ladybird Harmonia axyridis (Pallas)Fischer, Christophe  ; Laurent, Pascal ; Heuskin, Stéphanie et alin American Journal of Analytical Chemistry (2012), 3 These last few years the multicolored Asian ladybird, Harmonia axyridis (Pallas), previously introduced in Europe and North America as a biological control agent, has swiftly spread out on those ... [more ▼] These last few years the multicolored Asian ladybird, Harmonia axyridis (Pallas), previously introduced in Europe and North America as a biological control agent, has swiftly spread out on those territories and turns out to be a pest in several ways. In order to improve understanding of several key points of the biology of this ladybird, a novel fast-GC method of quantification of harmonine, the main defense alkaloid of H. axyridis, has been designed and validated using the accuracy profile concept for concentrations ranging from 13 to 264 µg/individual. This method allows quantifying harmonine in a single insect with a maximal error risk of 20%. For ladybirds collected in spring, mean harmonine concentration observed was 106.6 µg/individual with a very high standard deviation of 80.2 µg/individual, which can easily be explained by the strong asymmetry of the results distribution. Males and females seem equally chemically protected as the harmonine concentration did not significantly differ between sexes. This method is currently used to improve our comprehension of several key points of H. axyridis biology including aggregation behavior and aposematism. [less ▲] Detailed reference viewed: 44 (6 ULg)Fast-GC quantification of harmonine, the major defense alkaloid of the multicolored asian ladybird Harmonia axyridis (Pallas)Fischer, Christophe ; ; Heuskin, Stéphanie et alin American Journal of Analytical Chemistry (2012), 3 Detailed reference viewed: 4 (3 ULg) |
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