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See detailEnhancement of bovine leukemia virus-induced syncytia formation by di- and tripeptides.
Voneche, V.; Callebaut, I.; Lambrecht, B. et al

in Virology (1993), 192(1),

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by ... [more ▼]

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by bovine leukemia virus (BLV). Peptides containing a hydrophobic contribution and/or a positive net charge strongly enhanced syncytia formation induced by BLV on CC81 indicator cells. The size of the multinucleated cells was strongly increased (up to 10-fold) in the presence of the enhancer peptides whereas no effect was observed on the indicator cells in the absence of BLV. The peptides thus amplified the fusion process initiated by BLV envelope glycoproteins. The effect was dose-dependent at concentrations ranging from 20 to 640 microM and did not result from an increased expression of BLV proteins. The peptides did not compete with anti-gp51 monoclonal antibodies for the recognition of eight well-defined epitopes of gp51. We consequently hypothesize that the enhancer peptides interact with the membrane of BLV-producing cells and/or indicator cells and propose a model based on molecular modeling. [less ▲]

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See detailGenetic Relationships between Bovine Herpesvirus 4 and the Gammaherpesviruses Epstein-Barr Virus and Herpesvirus Saimiri
Bublot, M.; Lomonte, P.; Lequarré, Anne-Sophie ULg et al

in Virology (1992), 190(2), 654-65

The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at least ... [more ▼]

The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. All of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simplex virus type 1 or the betaherpesvirus human cytomegalovirus. The genome organization of BHV-4 had also an overall colinearity with that of the gammaherpesviruses EBV and HVS. Furthermore, the BHV-4 genes content and arrangement were more similar to those of HVS than to those of EBV, suggesting that BHV-4 and HVS are evolutionarily more closely related to each other than either are to EBV. BHV-4 DNA sequences were generally deficient in CpG dinucleotide. This CpG deficiency is characteristic of gammaherpesvirus genomes and suggests that the BHV-4 latent genome is extensively methylated. Despite several biological features similar to those of betaherpesviruses, BHV-4 displays the molecular characteristics of the representative members of the gammaherpesvirinae subfamily. [less ▲]

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See detailIn vivo transfection of bovine leukemia provirus into sheep.
Willems, Luc ULg; Portetelle, Daniel ULg; Kerkhofs, P. et al

in Virology (1992), 189(2),

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See detailThe Bovine Leukemia-Virus Tax Gene Contains An Enhancer Sequence
Willems, Luc ULg; Romond, Pc.; Ghysdael, J. et al

in Virology (1991), 182(1),

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See detailUse Of Synthetic Peptides To Map Sequential Epitopes Recognized By Monoclonal-Antibodies On The Bovine Leukemia-Virus External Glycoprotein
Callebaut, I.; Burny, A.; Krchnak, V. et al

in Virology (1991), 185(1),

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See detailThe coding region for the 54-kDa protein of several pestiviruses lacks host insertions but reveals a "zinc finger-like" domain
De Moerlooze, L.; Desport, M.; Renard, A. et al

in Virology (1990), 177(2), 812-5

We sequenced cDNAs, amplified by the polymerase chain reaction (PCR), which correspond to the carboxy-terminal portion of the 54-kDa protein of various cytopathic (cp) or noncytopathic (ncp) pestiviral ... [more ▼]

We sequenced cDNAs, amplified by the polymerase chain reaction (PCR), which correspond to the carboxy-terminal portion of the 54-kDa protein of various cytopathic (cp) or noncytopathic (ncp) pestiviral strains. Except for the previously described insertions in two cp strains of the bovine viral diarrhea virus (BVDV), we did not find comparable insertions in this gene in eight pestiviral strains. The predicted amino acid sequences of this 54-kDa protein portion contain a conserved cysteine-rich stretch remarkably similar to a "zinc finger-type" binding domain found in many gene-regulatory proteins. Thus, this protein may be involved in the binding to viral RNA. [less ▲]

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See detailSynthetic Peptides Approach To Identification Of Epitopes On Bovine Leukemia-Virus Envelope Glycoprotein-Gp51
Portetelle, Daniel ULg; Dandoy, C.; Burny, A. et al

in Virology (1989), 169(1),

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See detailAntigenic Variants Of Bovine Leukemia-Virus (Blv) Are Defined By Amino-Acid Substitutions In The Nh2 Part Of The Envelope Glycoprotein-Gp51
Portetelle, Daniel ULg; Couez, D.; Bruck, C. et al

in Virology (1989), 169(1),

Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a ... [more ▼]

Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a yeast-E. coli shuttle vector carrying the PHO5 promoter, the p24 gene and the CYC1 transcription terminator. After low inorganic phosphate (Pi) induction of the PHO5 promoter, p24 accumulated in the producing cells up to a concentration representing 10% of total soluble proteins. The expression level of p24 gene was not increased by insertion of the positive regulatory gene PHO4 on the p24 expression vector. The p24 produced in this system and incubated in crude yeast extract showed a remarkably high resistance to proteolytic degradation, a feature that presumably correlates with the compact globular conformation of the protein combined to the stabilizing effect of the N-terminal residue. [less ▲]

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See detailExpression of a cDNA clone corresponding to the long open reading frame (XBL-I) of the bovine leukemia virus.
Willems, Luc ULg; Bruck, C.; Portetelle, Daniel ULg et al

in Virology (1987), 160(1), 55-9

Nucleotide sequence analysis of a cDNA clone corresponding to the XBL-I open reading-frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that ... [more ▼]

Nucleotide sequence analysis of a cDNA clone corresponding to the XBL-I open reading-frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the env gene and was part of the p34x mRNA splice donor. . .ATGG/GTAA at the end of the pol gene sequence. RNA from this clone was synthesized in vitro by the SP6 RNA polymerase and translated into a 34,000 mol wt protein in rabbit reticulocyte lysates. The protein (p34x) is recognized in Western blots by most sera of BLV-infected sheep and tumor-bearing cattle, by an anti-synthetic peptide rabbit serum, and by the serum of a rabbit immunized by XBL-I RNA programmed reticulocyte lysates. Both sera react with a 34,000 mol wt protein present in nuclei of BLV-infected cells. [less ▲]

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See detailBovine Leukemia Virus (BLV) - A structural model based on chemical crosslinking studies
Uckert, W.; Wunderlich, V.; Ghysdael, Jacques et al

in Virology (1984), 133

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See detailTopographical analysis by monoclonal antibodies of BLV-gp51 epitopes involved in viral functions.
Bruck, Claudine; Portetelle, Daniel ULg; Burny, Arsène et al

in Virology (1982), 122

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See detailGenomic Divergence among Sindbis Virus Strains
Rentier-Delrue, Françoise ULg; Young, N. A.

in Virology (1980), 106

Antigenic variants of the alphavirus Sindbis strains have been isolated from the Paleartic, Ethiopian, Oriental, and Australian zoogeographic regions. The genome of these variants were analyzed for ... [more ▼]

Antigenic variants of the alphavirus Sindbis strains have been isolated from the Paleartic, Ethiopian, Oriental, and Australian zoogeographic regions. The genome of these variants were analyzed for homology by hybridization of virion RNAs to double-stranded RNAs isolated from infected cells. Under nonstringent conditions (Tm-55°) the RNA of Oriental-Australian strains showed only 35 to 51% nucleotide sequence homology with the RNAs of the Paleartic-Ethiopian strains although homology was essentially complete among isolates within the Oriental and Australian regions and among isolates within the Paleartic and Ethiopian regions. Under more stringent conditions (Tm-26°), nucleotide sequence differences of 2 to 45% were detected among the RNAs of virus strains from geographically distant localities within each of these two major zoogeographic subdivisions. Year of isolation, passage histoty and vertebrate or invertebrate host of origin were not major determinants of sequence heterology. The hypothesis that ancestral Sindbis virus became separated by geographic barriers and evolved into two distinct types is presented. Further divergent evolution has obviously occurred wihin each of these types. [less ▲]

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See detailProperties of the DNA product of reverse transcription of turnip yellow mosaic virus RNA preparations.
Demeure, M.; Kummert, Jean; Portetelle, Daniel ULg et al

in Virology (1977), 81

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