References of "Reproduction in Domestic Animals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailBreed-cross pregnancies increased plasmatic pregnancy-associated glycoprotein (PAG) concentration in pregnant Neospora-seropositive dairy cows
López-Gatius, Fernando; Garbayo, J. M.; Serrano, B. et al

in Reproduction in Domestic Animals (2006), 41(suppl 2), 112

Detailed reference viewed: 33 (4 ULg)
Full Text
Peer Reviewed
See detailThe Glycosylation of Bovine Pregnancy-Associated Glycoproteins Changes before Parturition
Klisch, K.; Herzog, K.; Feldmann, M. et al

in Reproduction in Domestic Animals (2006)

Detailed reference viewed: 18 (2 ULg)
Full Text
Peer Reviewed
See detailEffects of a single injection of a progesterone receptor antagonist during the third month of gestation in sheep
Taverne, M. A. M.; Regeling, J. I.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2006), 41(4), 310

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailSuitability of bovine pregnancy-associated glycoprotein (bPAG-1) for early pregnancy diagnosis in cows
Friedrich, M.; Kuwer, A.; Beckers, Jean-François ULg et al

in Reproduction in Domestic Animals (2005), 40(4), 274

Detailed reference viewed: 14 (3 ULg)
Full Text
Peer Reviewed
See detailEffect of the type and number of prostaglandin treatments on corpus luteum, the largest follicle and progesterone concentration in dairy cows
Repasi, A.; Beckers, Jean-François ULg; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2005), 40(5), 436-442

Lactating dairy cows (n = 72) with a mature corpus luteum (CL) (diameter of >= 17 mm) determined by ultrasonography and having a follicle with a diameter of >= 10 min were randomly assigned to four groups ... [more ▼]

Lactating dairy cows (n = 72) with a mature corpus luteum (CL) (diameter of >= 17 mm) determined by ultrasonography and having a follicle with a diameter of >= 10 min were randomly assigned to four groups. Cows were treated with cloprostenol i.m. once or twice, or with dinoprost i.m. once or twice with an 8-h interval. The ovaries of each cow were scanned daily by transrectal ultrasonography to measure the changes in the areas of CL and the largest follicle and to determine the occurrence of ovulation. Oestrus was verified twice daily. In addition, blood sample was withdrawn from each cow daily for measuring progesterone (P4) concentrations. Significant decreases in the percentage changes relative to areas of CL and P4 concentrations or increases in the percentage changes in the area of the largest follicle on day 0 were detected in each group during the experiment. However, the type of the drug and the number of the treatments had no significant effect on those parameters. Cows ovulated with or without showing oestrus (group A) and cows exhibiting no oestrus and ovulation (group 13) were also evaluated. In contrast to the mean area of the CL and the mean concentration of P4 on day 0, the mean area of the largest follicles between, the two groups on day 0 differed significantly Significant decreases in the percentage changes relative to the area of the CL and P4 concentration or increases in the percentage changes relative to the area of the largest follicle during the experiment were detected in both groups; however, there were no group differences. Treatment of dairy cows with two injections of prostaglandins (cloprostenol or dinoprost) at an 8-h interval resulted in more cows being, observed in oestrus within 5 days after treatment and having significantly higher pregnancy rate than those treated with a single prostaglandin injection. [less ▲]

Detailed reference viewed: 26 (1 ULg)
Full Text
Peer Reviewed
See detailThe viability of in vitro produced sheep embryos is negatively affected by the presence of estradiol during in vitro maturation
Poulin, N.; Cognié, Y.; Baril, G. et al

in Reproduction in Domestic Animals (2005), 40(4), 92

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailEmbryonic signals and pregnancy diagnosis in ruminants
Beckers, Jean-François ULg; Touati, Kamal ULg; Alvarez-Oxiley, A. V. et al

in Reproduction in Domestic Animals (2004), 39(4), 252

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailOvine pregnancy-associated glycoprotein profiles in ewes pregnant with singletons or twins
Vandaele, L.; Verberckmoes, S.; Van Soom, A. et al

in Reproduction in Domestic Animals (2004), 39(4), 278

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailUse of pregnancy specific proteins, P4, PGFM and E1S assays to monitor pregnancy in sheep
Gajewski, Z.; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Reproduction in Domestic Animals (2004), 39(4), 288

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailNew possibilities for the diagnosis of early pregnancy (EP) and late embryonic mortality (LEM) in the cow
Szenci, O.; Taverne, M. A. M.; Beckers, Jean-François ULg

in Reproduction in Domestic Animals (2004), 39(4), 252

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailEffect of different doses of prostaglandin on the area of corpus luteum, the largest follicle and progesterone concentration in the dairy cow
Repasi, A.; Beckers, Jean-François ULg; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2003), 38(6), 423-428

Lactating dairy cows with a mature corpus luteum (CL) (diameter of greater than or equal to17 mm determined by ultrasonography) and having a follicle with a diameter of 10 mm (n = 49) were randomly ... [more ▼]

Lactating dairy cows with a mature corpus luteum (CL) (diameter of greater than or equal to17 mm determined by ultrasonography) and having a follicle with a diameter of 10 mm (n = 49) were randomly assigned to three groups. The first group was treated with a single dose of exogenous prostaglandin (25 mg), while the second group was treated with 35 mg on day 0, and the third group served as control in order to evaluate the effect of rectal manipulation on the CL during ultrasonographic examination. Blood samples were collected daily for analysing progesterone (P4) concentrations. In group 1 the incidence of oestrus and artificial insemination (AI) in 10 days after treatment was 95% (19 of 20). The conception rate was 31.6%, and the average time to oestrus after treatment was 3.7 day. In group 2 the incidence of oestrus and A.I. was 84.2% (16/19). The conception rate was 31.2%, and the average time to oestrus after treatment was 2.8 day. In the untreated group only two cows (2/10) exhibited oestrus during the examined period and none of them became pregnant. There were no significant differences between the two treated groups in terms of reduction in the area of CL and P4 concentrations and of an increase in the area of the dominant follicles. At the same time, the decrease in the percentage changes relative to the area of CL and to the concentrations of P4 were statistically significant in both treated groups. [less ▲]

Detailed reference viewed: 12 (2 ULg)
Full Text
Peer Reviewed
See detailPregnancy-associated glycoprotein concentrations in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep.
El Amiri, B.; Cognié, Y.; Sulon, J. et al

in Reproduction in Domestic Animals (2003), 38(4), 322

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailValidation of two homologous radioimmunoassays for measuring pregnancy-associated glycoprotein in ewes.
El Amiri, B.; Karen, A.; Sulon, J. et al

in Reproduction in Domestic Animals (2003), 38(4), 358

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailPAG profiles in postpartum dairy cows and newborn calves
Ropstad, E; Kalberg, K; Dahl, E et al

in Reproduction in Domestic Animals (2002), 37(4), 249

In ruminants, pregnancy associated glycoproteins (PAG), synthesized in the outer layers of the trophoblast, enter the maternal bloodstream. In this study, blood samples from cows in the postpartum period ... [more ▼]

In ruminants, pregnancy associated glycoproteins (PAG), synthesized in the outer layers of the trophoblast, enter the maternal bloodstream. In this study, blood samples from cows in the postpartum period, pregnant cows and calves were collected to determine 1) the clearance rate of PAG, 2) the accuracy of PAG measurements used for pregnancy diagnosis and 3) PAG concentrations and clearance rate in newborn calves. A curvilinear decline in PAG was found with increasing days from calving. Between day 70–80 pp 3 out of 11 samples (27%) had PAG concentrations > 4 ng/ml plasma. A cut-off value of 4 ng/ml was found to give the best accuracy for pregnancy diagnosis 28–36 days after service (n ¼58: sensitivity¼0.97; specificity ¼1.0). PAG concentrations in newborn calves (n¼10) varied between 78–880 ng/ml, declining with increasing age in a curvilinear pattern to < 1 ng/ml between 20–40 days pp. In two male twin calves PAG concentrations were 880 and 101 ng/ml, respectively, at one day of age. We conclude: 1) 4 ng PAG /ml provides the best cut-off value for pregnancy diagnosis. 2) Cows serviced before 60 days pp can produce false positive pregnancy diagnoses due to PAG originating from the previous pregnancy. 3) High PAG concentrations can be found in newborn calves. [less ▲]

Detailed reference viewed: 1 (0 ULg)
Full Text
Peer Reviewed
See detailComparison between transrectal ultrasonography and pregnancy-associated glycoprotein determination for early pregnancy diagnosis in the goat
Quesada, E; Gonzalez, F; Calero, P et al

in Reproduction in Domestic Animals (2002), 37(4), 247

The aim of the present workwas to compare the efficiency of transrectal ultrasonography technique and the determination of the pregnancy-associated glycoproteins (PAG) concentrations in plasma samples for ... [more ▼]

The aim of the present workwas to compare the efficiency of transrectal ultrasonography technique and the determination of the pregnancy-associated glycoproteins (PAG) concentrations in plasma samples for pregnancy diagnosis in goats. Thirty nine Canary goats were synchronized and naturally mated every 12 hours throughout oestrous. Blood samples were collected on days 17, 18, 19, 20, 22, 24, 26 and 28 and transrectal ultrasonography was carried out on days 20, 22, 24, 26 and 28 (Day 0¼day of the last mating). PAG concentrations were analysed by a RIA method previously validated. There were 23 pregnant goats (10 single and 13 multiple pregnancies) and 16 nonpregnant goats. The pregnancy diagnosis was a little earlier by PAG determination than transrectal ultrasonography (sensitivity: 95 and 91% on Day 24 and 100 and 96% on Day 26, respectively). The ultrasonography allowed to discriminate between single and multiple pregnancies in 70% of goats (16/23) on Day 28 of pregnancy. The PAG concentrations were significantly higher in multiple than single pregnancies from Day 19 onwards, suggesting that it could also be used as a tool to discriminate between single and multiple gestations. (Supported by D.G. de Universidades del Gobierno de Canarias). [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailComparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma
Perenyi, Z. S.; Szenci, Otto; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2002), 37(2), 100-104

Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression pattern. When PAGs are released in the maternal blood they can be used for pregnancy diagnosis, pregnancy follow-up and for the monitoring of the trophoblastic function. Three different radioimmunoassay systems (RIA 1, RIA 2 and RIA 3) using antisera produced against PAG I67 (RIA 1), PAG55+62 (RIA 2) and PAG55+59 (RIA 3) were used in this investigation in order to measure the PAG concentration in plasma samples withdrawn from pregnant cows and heifers during different periods following artificial insemination (AI). These systems were able to detect PAG molecules in the maternal blood as early as 21 days after AI in different concentrations (RIA 1: 0.43 +/- 0.24 ng/ml, mean +/- SD; RIA 2: 0.48 +/- 0.24 ng/ml; RIA 3: 0.64 +/- 0.37 ng/ml). On days 32 and 42 RIA 2 (4.30 +/- 1.32 ng/ml and 5.56 +/- 1.95 ng/ml) and RIA 3 (4.17 +/- 1.15 ng/ml and 5.60 +/- 1.89 ng/ml) presented significantly (p < 0.0001) higher PAG concentrations than those of RIA 1 (2.43 +/- 0.81 ng/ml and 4.01 +/- 1.48 ng/ml), respectively. After day 21, significant correlations (p < 0.0001; r >/= 0.929) were determined between the three systems. Additionally the three individual PAG profiles presented in this study showed that PAG molecules secreted in the maternal blood between 21 and 50 days after AI were better recognized by the RIA 2 and RIA 3 systems. This study clearly indicated that the ability of a RIA test to recognize PAG molecules in the maternal blood can be improved by carefully selecting the antiserum. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailAspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins
Perenyi, Z. S.; Szenci, O.; Drion, Pierre ULg et al

in Reproduction in Domestic Animals (2002), 37(6), 324-329

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml - 1 mg/ml). Pepsinogen cross-reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 microg/ml and 500 microg/ml concentrations, respectively. In the presence of pepsin, cross-reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 microg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross-react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross-reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. [less ▲]

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailBovine pregnancy-associated glycoprotein profiles as indicators of trophoblastic function after in vitro manipulation or culture
Ectors, Fabien ULg; Sulon, José; Delval, A. et al

in Reproduction in Domestic Animals (1997, February), 32(1-2), 52

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailThe ovarian follicle in cow: in vivo growth and in vitro culture
Beckers, Jean-François ULg; Drion, Pierre ULg; Figueiredo, J. R. et al

in Reproduction in Domestic Animals (1996), 31

Detailed reference viewed: 80 (10 ULg)
Full Text
Peer Reviewed
See detailEffects of repeated use of progestagen-PMSG treatment for estrus control in dairy goats out of breeding season
Baril, Gérard; Remy, Benoît ULg; Vallet, J. C. et al

in Reproduction in Domestic Animals (1992), 27(3), 161-168

Contents: In order to analyze the effects of repeated use of progestagen-PMSG treatment, estrus and pregnancy results have been analyzed for 1989 in a Saânen dairy goat herd in which breeding takes place ... [more ▼]

Contents: In order to analyze the effects of repeated use of progestagen-PMSG treatment, estrus and pregnancy results have been analyzed for 1989 in a Saânen dairy goat herd in which breeding takes place each year out of season after FGA/PMSG treatment. After the first 1989 treatment (169 goats), percentage of goats showing estrus and kidding have been lower for 59 multiparous than for 46 primiparous and 64 nulliparous females. Moreover, when 38 goats are treated for a second time in 1989, 44.7% exhibited estrus vs 71.0% after the first treatment (P < 0.05). The PMSG binding level before the 1st 1989 treatment is higher for multiparous (17.5 ± 23.1%) than nulli and primiparous (-0.06 ± 0.7 and 1.2 ± 1.9%) and is increased for all parities after treatment (23.2 ± 26.4 after vs 5.7 ± 15.0% before, P < 0.01). For nulliparous and primiparous females; PMSG binding levels are not different for pregnant or not pregnant nulliparous and primiparous goats. On the opposite, PMSG binding rates are higher in non pregnant (25.7 ± 23.3) than in pregnant multiparous goats (6.5 ± 15.9) (P < 0.01). However, when the binding rate is ≤ 5.12% (computerized distributions) multiparous goats exhibit estrus and pregnancy at levels not different from nulliparous or primiparous females (% estrus 95.8 vs 100 or 97.8%, % pregnancy 66.7 vs 70.3 and 63.0% respectively). Repeated use of PMSG during the female life or during one given year leads to active immunization against PMSG (as measured by percentage of binding of PMSG in plasma) decreasing the efficiency of ovarian stimulation out of breeding season. [less ▲]

Detailed reference viewed: 33 (1 ULg)