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See detailFactors Affecting Plasma Pregnancy-associated Glycoprotein 1 Concentrations Throughout Gestation in High-producing Dairy Cows.
Serrano, B.; Lopez-Gatius, F.; Santolaria, P. et al

in Reproduction in Domestic Animals (2009), 44(4), 600-605

This study was designed to establish the factors, if any, which could affect plasma pregnancy-associated glycoprotein-1 (PAG-1) expression in a study population of 87 pregnant, high-producing dairy cows ... [more ▼]

This study was designed to establish the factors, if any, which could affect plasma pregnancy-associated glycoprotein-1 (PAG-1) expression in a study population of 87 pregnant, high-producing dairy cows. The factors examined were: semen providing breed (Holstein-Friesian vs Limousin), outcome of gestation (male vs female newborn, and singleton vs twin pregnancies), lactation number, milk production at pregnancy diagnosis, plasma progesterone concentration, season of gestation (warm period, March-November vs cool period, December-February), and day of gestation (40, 90, 120, 150, 180 and 210). Pregnancy was diagnosed by transrectal ultrasound on day 40 post-insemination and by palpation per rectum on days 90, 120, 150, 180 and 210. Blood samples were collected from each animal immediately before each pregnancy diagnosis. The relative contributions of the different factors on PAG-1 concentrations were evaluated by GLM repeated measures analysis of variance. No significant effects of the herd, foetal sex, milk production, lactation number and plasma progesterone concentrations were observed. In contrast, twin pregnancy, the use of Limousin semen and conception during the cool period were correlated with significantly increased plasma PAG-1 concentrations throughout gestation. Our data indicate that both cow well-being during early placental development, determined in our conditions by reduced heat stress when conception occurred in the cool season, and crossbreed pregnancies lead to improved PAG-1 production throughout the gestation period. [less ▲]

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See detailAnomalous Pregnancies during Late Embryonic/Early Foetal Period in High Producing Dairy Cows.
Serrano, B.; Lopez-Gatius, F.; Hunter, R. et al

in Reproduction in Domestic Animals (2009), 44(4), 672-676

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1 ... [more ▼]

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations. Data derived from a large-scale ultrasound pregnancy diagnosis programme in high producing dairy cows. Over a 3-year period (2004-2007), a very low incidence (0.5%: 15 of 3094) of anomalous pregnancies was recorded. The results revealed that the following anomalies were detected on days 35-41 of gestation in cows carrying singletons with one single corpus luteum: embryo death in eight cows (0.3%); and embryo in the uterine horn contralateral to the corpus luteum in seven cows (0.2%). All these animals suffered pregnancy loss during the early foetal period. In cows carrying dead embryos, no signs of conceptus degeneration were observed on pregnancy diagnosis. Amnion size (approximately 25 mm diameter) and uterine horn fluid contents were estimated to be similar to those of the normal pregnant cows in this period. In the contralateral gestations, live embryos were observed in all ultrasound checks before pregnancy loss. Uterine fluid contents increased in the two cows in which gestation continued for more than a week. In the cases of embryo death but not in those of contralateral gestation, a drop in PAG-1 levels was noted prior to pregnancy loss. Two cows carrying dead embryos increased with time allantoic fluid contents. The PAG-1 values increased with time in one cow bearing a dead embryo (from 2.31 to 6.79 ng/ml) and in two of the contralateral gestations (from 1.66 to 2.33 ng/ml and from 0.39 to 6.79 ng/ml, respectively). Results of this study indicate that the foetal membranes continue to undergo some activity following embryo death, and that contralateral pregnancy may determine failure of the gestation process. [less ▲]

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See detailSerum Profiles of Pregnancy-Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle.
Lobago, F.; Bekana, M.; Gustafsson, H. et al

in Reproduction in Domestic Animals (2009), 44(4), 685-692

Contents This study presents serum concentrations of pregnancy-associated glycoprotein (PAG), oestrone sulphate (E1-S) and progesterone (P4), and the effects of some dam and foetus-related factors on ... [more ▼]

Contents This study presents serum concentrations of pregnancy-associated glycoprotein (PAG), oestrone sulphate (E1-S) and progesterone (P4), and the effects of some dam and foetus-related factors on these profiles during gestation in Borana and crossbred cattle. The PAG concentrations at 4th week post-conception ranged from 1.5-5.5 and 2.1-4.7 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The mean PAG concentrations increased progressively from 4th to 33rd week of gestation (from 3.3-173 ng/ml for Borana and 4.2-240 ng/ml for crossbred cattle) and reached peak around calving. Breed, parity status, dam body weight, foetal sex and foetal birth weight significantly influenced the PAG concentrations. After delivery, the PAG concentrations declined steadily to 5.7 ng/ml in Borana (n = 7) and 3.9 ng/ml in crossbred (n = 6) cattle 10 weeks post-partum. The serum E1-S concentrations at 17th week of pregnancy ranged from 0.3-2.6 and 0.9-5.7 ng/ml in Borana (n = 8) and crossbred (n = 9) cattle, respectively. The mean E1-S concentrations increased progressively from 17th to 33rd week of gestation (from 1.1-4.6 ng/ml for Borana and 2.7-10.8 ng/ml for crossbred). Breed, parity status, dam body weight and foetal sex significantly influenced E1-S concentrations. The P4 concentrations at 4th week of pregnancy ranged from 3.2-5.1 and 1.7-8.9 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The P4 level remained elevated throughout pregnancy. This study indicated that the serum PAG and P4 concentrations at 4th and E1-S approximately 17th week of pregnancy were above the cut-off value for pregnancy test and the hormonal profiles observed were comparable to the previous reports. Furthermore, the PAG and E1-S profiles were considerably influenced by factors such as breed, weight and parity status of the dam, and foetal sex and foetal birth weight (only PAG). [less ▲]

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See detailEffects of intraovarian application of insulin-like growth factor-1 (IGF-1) on the superovulatory response of diary cattle.
Velazquez, M. A.; Hadeler, K. G.; Beckers, Jean-François ULg et al

in Reproduction in Domestic Animals (2009), 44(S1), 38

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See detailRecent possibilities for diagnosing early pregnancy (EP) in small ruminants and pigs.
Szenci, O; Beckers, Jean-François ULg; Taverne, M.A.M

in Reproduction in Domestic Animals (2008), 43(5), 104

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See detailPregnancy diagnosis in the mare by semi quantitative relaxin quick assay kit
Ponthier, Jérôme ULg; van de Weerdt, marie-Lys; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2008, July), 43(s3), 111

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See detailEffects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production.
De Roover, R.; Feugang, J. M.; Bols, P. E. J. et al

in Reproduction in Domestic Animals (2008), 43(2), 239-245

The aim of this retrospective study was to compare the number of follicles, cumulus oocyte complexes (COCs) and cultured In Vitro Produced (IVP) embryos obtained from 1396 non-stimulated Ovum Pick-up (OPU ... [more ▼]

The aim of this retrospective study was to compare the number of follicles, cumulus oocyte complexes (COCs) and cultured In Vitro Produced (IVP) embryos obtained from 1396 non-stimulated Ovum Pick-up (OPU) sessions on 81 donor animals in a twice weekly OPU scheme. Results were obtained from 640 sessions following FSH-LH superstimulation, on 112 donors subjected to OPU once every 2 weeks. The stimulation protocol started with the insertion of an ear implant containing 3 mg norgestomet (Crestar, Intervet, Belgium) 8 days before puncture (day -8). The dominant follicle was ablated by ultrasound-guided follicle puncture on day -6. On day -3 and day -2, cows were injected with FSH (Ovagen, ICP) twice daily (8 am to 8 pm), i.e. a total dose of 160 mug FSH and 40 mug LG per donor per stimulation cycle. Animals were punctured 48 h after the last FSH injection (day 0). Progesterone implants were removed the next day. Stimulated donor cows were treated with this protocol at 14-day intervals. Follicles were visualized with a Dynamic Imaging ultrasound scanner, equipped with a 6.5 MHz sectorial probe. Follicles were punctured with 55 cm long, 18 gauge needles at an aspiration pressure corresponding to a flow rate of 15 ml/min. Cumulus oocyte complexes were recovered and processed in a routine IVF set-up. Results demonstrate that, expressed per session, FSH stimulation prior to OPU increases production efficiency with significantly more follicles punctured and oocytes retrieved. However, when overall results during comparable 2-week periods are considered (four non-stimulated sessions vs one stimulated), more follicles are punctured and more oocytes are retrieved using the non-stimulated protocol. No significant differences in the number of cultured embryos could be detected, indicating that FSH/LH stimulation prior to OPU might have a positive effect on in vitro oocyte developmental competence as more embryos are cultured with less, presumably better-quality, oocytes [less ▲]

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See detailPregnancy-associated glycoprotein secretion in North Moroccan goats
Chentouf, M.; El Amiri, B.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2008), 43(6), 696-700

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral ... [more ▼]

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral blood was collected every day during the first month of pregnancy, afterward the blood samples were collected trice a week. The PAG were first detected at day 20 of pregnancy, their levels increase to week 8 of gestation then decrease slightly until parturition. Statistical differences between goats carrying one or two foetuses are observed from week 5 of pregnancy until parturition. Factorial Discriminant Analysis provides mathematical models for the discrimination between the litter size using the PAG level and the week of pregnancy. Using only one blood sample per week, high sensitivity, specificity and overall accuracy (74%, 88% and 81%) were obtained using these models. It is concluded that the PAG RIA is an effective tool for early diagnosis of pregnancy and for discrimination between the litter sizes in North Moroccan goats. [less ▲]

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See detailNo effect of parity on concentrations of bovine pregnancy associated glycoprotein (PAG) measurement by radioimmunoassay.
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 90

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See detailCorrelation between five radioimmunoassay systems for bovine pregnancy associated glycoprotein (PAG).
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 90

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See detailPregnancy diagnosis in ruminant species.
Melo de Sousa, Noelita ULg; Szenci, O.; Taverne, M. A. M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 104

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See detailAssessment of three ovulation induction protocols in mares (hCG, Buserelin and Crude Equine Gonadotropin)
Mourier, E.; Ponthier, Jérôme ULg; Beckers, Jean-François ULg et al

in Reproduction in Domestic Animals (2007, September), 42(Suppl. 2), 76

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See detailInfluence of continuous breeding goats on return to cyclicity in seasonal breeding goats
Prvanovic, N; Vince, S; Duricic, D et al

in Reproduction in Domestic Animals (2007), 42

The boer goats which originated from the South African Republic are continous breeding animals when kept in the northern hemisphere and they kidd three times in 2 years. In this study, a mixed herd was ... [more ▼]

The boer goats which originated from the South African Republic are continous breeding animals when kept in the northern hemisphere and they kidd three times in 2 years. In this study, a mixed herd was monitored to determine whether boer goats could induce return to cyclicity in sesonal breeding goats. All goats (n = 20) were pluriparous and kidded during January and February. During January a mixed group was formed (10 boer goats kept together with 10 domestic breed goats). At the same time a group of domestic breed goats (n = 12), kept in the same manner, 40 km away from the experimental group was used as controls. Blood samples were collected every 72 h to determine level of progesterone in both herds. Goats were monitored at the same time to observe for signs of oestrus. Progesterone concentrations in sera samples were determined using a RIA method. Data were analysed by ANOVA using Statistica software. We observed visual oestrus in all boer goats and in eight domestic breed goats from the mixed group during March and April. Progesterone concentrations confirmed the presence of functional corpora lutea between two visual cycles in these animals. No visual oestrus or increases in progesterone were observed in the control group of goats. In conclusion, continuous breeding boer goats induced oestrus in 80% (n = 8) of seasonal breeding goats when kept in a mixed herd. [less ▲]

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See detailMeasurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep
El Amiri, B.; Karen, A.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2007), 42(3), 257-262

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer ... [more ▼]

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG(57+59kDa)) and R805 (against ovPAG5(58+61kDa)) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p < 0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding. [less ▲]

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See detailComparison of five radioimmunoassay systems for PAG measurement: Ability to detect early pregnancy in cows
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2007), 42(4), 433-440

This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems ... [more ▼]

This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems: RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool. Their ability to distinguish between non-pregnant and pregnant females at day 30 after artificial insemination (Al) was investigated. The antisera were raised in rabbits against different PAG preparations. All RIA systems proved to be sensitive, repeatable and accurate for measuring PAG concentrations. The dilutions of plasma samples taken at an early stage of pregnancy were found to be parallel to the standard curves. No cross-reaction was observed with different carbohydrates, either with Pregnant Marc Serum Gonadotropin (PMSG) or human Chorionic Gonadotropin (hCG). The concentrations of PAG in pregnant females at day 30 after Al were shown to be higher with the use of antisera R#706, R#780, R#809 and Pool when compared with antiserum R#497. All the RIA systems gave 100% sensitivity and negative predictive values. On the other hand, the use of antisera R#780 and R#809 resulted in lower specificity and positive predictive values. The present study clearly shows that the ability of PAG-RIA systems to diagnose pregnancy specifically at day 30 after Al can be improved by using a combination of antisera raised against different forms of PAG. [less ▲]

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See detailApproaches to unravel the pathophysiological background of unexplained perinatal mortality in dairy cows
Taverne, M. A. M.; van der Weijden, G. C.; Jonker, F. H. et al

in Reproduction in Domestic Animals (2007), 42(Suppl. 2), 64

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See detailBreed-cross pregnancies increased plasmatic pregnancy-associated glycoprotein (PAG) concentration in pregnant Neospora-seropositive dairy cows
López-Gatius, Fernando; Garbayo, J. M.; Serrano, B. et al

in Reproduction in Domestic Animals (2006), 41(suppl 2), 112

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See detailThe Glycosylation of Bovine Pregnancy-Associated Glycoproteins Changes before Parturition
Klisch, K.; Herzog, K.; Feldmann, M. et al

in Reproduction in Domestic Animals (2006)

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See detailEffects of a single injection of a progesterone receptor antagonist during the third month of gestation in sheep
Taverne, M. A. M.; Regeling, J. I.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2006), 41(4), 310

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