References of "Clinical Chemistry & Laboratory Medicine"
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See detailA new statistical method for evaluating long-term analytical performance of laboratories applied to an external quality assessment scheme for flow cytometry.
Coucke, Wim; Van Blerk, Marjan; Libeer, Jean*-Claude et al

in Clinical Chemistry & Laboratory Medicine (2010), 48(5), 645-50

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the ... [more ▼]

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the target and reported values of each parameter for each laboratory during the past 3 years. This study aims to develop a method to find laboratories with aberrant variability or bias using robust techniques and to obtain robust estimates of the variability. METHODS: A method is proposed to find outliers with respect to the individual regression line, followed by a step to find regression lines with excessive variability and finally a step to find regression lines with high bias. RESULTS: The model was applied to the results obtained by 52 laboratories for CD4%. From the 1340 data points, 35 were determined to be regression outliers. The second step revealed one regression line with excessive variability; the third step detected three regression lines with exceeding bias. CONCLUSIONS: The methodology allows assessment of the long-term performance of laboratories, taking into account samples with different target values. Outliers in the first step indicate accidental mistakes, outliers in the second and third step point to high analytical variability or bias. [less ▲]

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See detailANALYTICAL PERFORMANCE OF THE C-TERMINAL FGF-23 (IMMUTOPICS)
Cavalier, Etienne ULg; Rozet, Eric ULg; Carlisi, Ignazia ULg et al

in Clinical Chemistry & Laboratory Medicine (2009, June), 47(M-A032),

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See detailVitamin D: current status and perspectives.
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Chapelle, Jean-Paul ULg et al

in Clinical Chemistry & Laboratory Medicine (2009), 47(2), 120-127

Abstract The role of vitamin D in maintaining bone health has been known for decades. Recently, however, the discovery that many tissues expressed the vitamin D receptor and were able to transform the 25 ... [more ▼]

Abstract The role of vitamin D in maintaining bone health has been known for decades. Recently, however, the discovery that many tissues expressed the vitamin D receptor and were able to transform the 25-OH vitamin D into its most active metabolite, 1,25-(OH)(2) vitamin D, has led to a very promising future for this "old" molecule. Indeed, observational studies, and more and more interventional studies, are raising the importance of a significant vitamin D supplementation for not-only skeletal benefits. Among them, 25-OH vitamin D has been found to play an important role in prevention of cancers, auto-immune diseases, cardiovascular diseases, diabetes, and infections. Vitamin D deficiency, defined as serum 25-OH vitamin D levels <30 ng/mL, is very common in our population. The cost/benefit ratio and some recently published studies are clearly now in favor of a controlled and efficient vitamin D supplementation in these patients presenting a 25-OH vitamin D level <30 ng/mL. More attention should also be focused on pregnant and lactating women, as well as children and adolescents. Clin Chem Lab Med 2009;47. [less ▲]

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See detailAn unusual interference in parathormone assay caused by anti-goat IgG: a case report.
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Ignazia ULg et al

in Clinical Chemistry & Laboratory Medicine (2009), 47(1), 118

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See detailCurrent practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme.
Van Blerk, Marjan; Van Campenhout, Christel; Bossuyt, Xavier et al

in Clinical Chemistry & Laboratory Medicine (2009), 47(1), 102-8

BACKGROUND: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian ... [more ▼]

BACKGROUND: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian National External Quality Assessment Scheme from 2000 to 2005. METHODS: During this period, nine samples with different specificities were sent for analysis. Participants were surveyed for methodology used and were asked to report staining pattern and titer of ANAs. In 2002, an attempt was made to improve the comparability of quantitative ANA results by the provision of a commercial reference material and to relate observed differences to methodology. RESULTS: With one exception, all participants employed a microscope-based indirect immunofluorescence assay with human epithelial cell line 2 cells. Most laboratories were accurate in describing the pattern. The percentage of unacceptable answers was greater for samples with borderline levels of antibody and for samples showing a cytoplasmic pattern. An improvement in the detection of anticentromere antibodies was observed. For all samples, a wide range of titers was reported. The provision of the secondary reference preparation led to improved inter-laboratory concordance. Comparison of methodology variables revealed a correlation between unstandardized titers and the power of the lamp of the microscope and the use of a dark room. CONCLUSIONS: The EQAS results presented in this work provide valuable insights into the state of the art of ANA testing as practiced in the Belgian and Luxembourg Laboratories and illustrate the important value of a national EQAS for ANA testing as a tool to improve performance and interlaboratory comparability of laboratory results. [less ▲]

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See detailCystatin C: current position and future prospects.
Séronie-Vivien, Sophie; Delanaye, Pierre ULg; Piéroni, Laurence et al

in Clinical Chemistry & Laboratory Medicine (2008), 46(12), 1664-1686

Abstract Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of cystatin C is mainly determined by ... [more ▼]

Abstract Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. This review summarises current knowledge about the physiology of cystatin C and about its use as a renal marker, either alone or in equations developed to estimate the glomerular filtration rate. This paper also reviews recent data about the other applications of cystatin C, particularly in cardiology, oncology and clinical pharmacology. Clin Chem Lab Med 2008;46:1664-86. [less ▲]

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See detailPTH stability : preanalytical conditios influences for Liaison (Diasorin)
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Ignazia ULg et al

in Clinical Chemistry & Laboratory Medicine (2007, June), 45

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See detailEvaluation of heterophile antibodies interference on Liaison PTH (Diasorin)
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Ignazia ULg et al

in Clinical Chemistry & Laboratory Medicine (2007, June), 45

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See detailAnalytical validation of the new version of the Liaison N-Tact PTH assay
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Agnès et al

in Clinical Chemistry & Laboratory Medicine (2007), 45(1), 105-107

We performed analytical validation of the new version of the Liaison N-Tact PTH (parathormone) assay according to NCCLS guidelines and compared this new generation of reagent with the Roche Elecsys PTH ... [more ▼]

We performed analytical validation of the new version of the Liaison N-Tact PTH (parathormone) assay according to NCCLS guidelines and compared this new generation of reagent with the Roche Elecsys PTH assay. We showed that this new version is a sensitive and precise method with good recovery. Significant carryover was observed, but with limited clinical implications. We demonstrated that the new version of the Liaison PTH is in reasonably good agreement with the Roche Elecsys PTH assay, and as we observed no differences in a hemodialyzed population, moving from one method to the other should not affect the daily follow-up of patients. However, one should be cautious with the highest values (> 500 pg/mL). We established reference intervals of 12-54 pg/mL for the Liaison and 14-52 pg/mL for the Elecsys assay. [less ▲]

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See detailAnalytical validation of the new plasma calibrated Accu-Chek (R) Test Strips (Roche Diagnostics)
Meex, Cécile ULg; Poncin, José ULg; Chapelle, Jean-Paul ULg et al

in Clinical Chemistry & Laboratory Medicine (2006), 44(11), 1376-1378

Background: The Accu-Chek Inform glucose monitor is a point-of-care system for testing blood glucose. New test strips, calibrated to deliver glucose plasma-like values, were launched on the market in May ... [more ▼]

Background: The Accu-Chek Inform glucose monitor is a point-of-care system for testing blood glucose. New test strips, calibrated to deliver glucose plasma-like values, were launched on the market in May 2005. The aim of our study was to perform analytical validation of these new strips. Methods: We compared the new plasma strips with whole blood strips; results for the plasma strips with plasma values obtained using a clinical analyzer and with whole blood values given by the glucose electrode of a blood gas analyzer; and the influence of the type of blood (capillary or venous) on the results obtained by the glucose monitor with the plasma calibrated strips. Results: Plasma strips give on average 7% higher results than the previous whole blood strips. However, the results given by the plasma strips on capillary whole blood, even if well correlated, are not completely comparable with those given by an analyzer for venous plasma. Nevertheless, these plasma strips and the glucose electrode of a blood gas analyzer give comparable results. Conclusions: Accu-Chek Inform plasma strips are a good method for monitoring of blood glucose values in patients with diabetes. [less ▲]

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See detailComparison of liaison N-tact PTH (diasorin) and N-tact PTH SPIRMA (diasorin) in hemodialyzed patients
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Krzesinski, Jean-Marie ULg et al

in Clinical Chemistry & Laboratory Medicine (2005), 43(8), 890-891

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See detailEnvironmental dichlorodiphenyltrichlorethane or hexachlorobenzene exposure and breast cancer: is there a risk?
Charlier, Corinne ULg; Foidart, Jean-Michel ULg; Pitance, François ULg et al

in Clinical Chemistry & Laboratory Medicine (2004), 42(2), 222-227

The carcinogenic potential of environmental xenoestrogens (i.e., organochlorines) is a matter of controversy. Their pathogenic role as promoters in breast cancer has been previously suggested. In Europe ... [more ▼]

The carcinogenic potential of environmental xenoestrogens (i.e., organochlorines) is a matter of controversy. Their pathogenic role as promoters in breast cancer has been previously suggested. In Europe, despite their prohibition since the '70s, residues persist in soil and rivers resulting in a widespread contamination of the general population. In this study, we have compared the serum levels of p,p'-1,1-dichloro-2, 2-bis (4-chlorophenyl) ethylene (DDE) and hexachlorobenzene (HCB) in 231 women at the time of breast cancer discovery and in 290 agematched healthy controls. p,p'-DDE was found in 76.2% of cases and in 71.1% of controls but HCB was present only in 12.6% of cases (29 from 231) and in 8.9% of controls (26 from 290). Even if taking all undetectable results (recorded as "0") into consideration, mean values were significantly different in cases when compared to controls. The serum level of p,p'-DDE was 3.46+/-3.48 ppb (0.58+/-0.58 mug/g lipid) in patients and 1.85+/-2.09 ppb (0.31+/-0.35 mug/g lipid) in controls (p<0.0001). The HCB serum level was 0.66+/-1.25 ppb (0.11+/-0.21 mug/g lipid) in patients and 0.20+/-1.02 ppb (0.03+/-0.17 mug/g lipid) in controls (p<0.0001). When considering p,p'-DDE and HCB as binary variables (1 if higher than the limit of quantification, 0 if lower), the presence of both residues was significantly associated with an increased risk of breast cancer development (OR 2.21, 95% CI 1.41-3.48 for p,p'-DDE and OR 4.99, 95% CI 2.95-8.43 for HCB). No excess was observed among parous women or when familial history of breast cancer was considered. In the cancer group, no differences in serum levels of p,p'-DDE or HCB were found in relation with estrogenreceptor (ER) status, Bloom stage or lymph node metastasis, but the HCB level was moderately correlated with tumor size (p=0.026). [less ▲]

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See detailThe use of the Tosoh AIA-600II and AIA-1800 immunoassay analysers for the determination of CTNI
Chapelle, Jean-Paul ULg; Aldenhoff, Marie-Claire ULg; Gielen, Jacques

in Clinical Chemistry & Laboratory Medicine (2003), 41

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See detailMultimarker approach to risk stratification
Chapelle, Jean-Paul ULg

in Clinical Chemistry & Laboratory Medicine (2003), 41

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See detailInfluence of Hyperbilirubinemia on Spectrophotometric Measurement of 30 Biochemical Tests, using the Roche® Modular P-module
Goffinet, Julie ULg; Bodson, Quentin; Chapelle, Jean-Paul ULg et al

in Clinical Chemistry & Laboratory Medicine (2003), 41

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See detailDetermination of organochlorine pesticide residues in the blood of healthy individuals
Charlier, Corinne ULg; Plomteux, Guy ULg

in Clinical Chemistry & Laboratory Medicine (2002), 40(4), 361-364

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See detailComparison of plasma cardiac troponins T and I in chronically hemodialyzed patients in relation to cardiac status and age
Chapelle, Jean-Paul ULg; Dubois, Bernard ULg; Bovy, Christophe ULg et al

in Clinical Chemistry & Laboratory Medicine (2002), 40(3), 240-245

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See detailAre cardiac markers useful in patients with chronic renal failure?
Chapelle, Jean-Paul ULg

in Clinical Chemistry & Laboratory Medicine (2001), 39(suppl), 36

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