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See detailIDS iSYS automated intact procollagen-1-Nterminus pro-peptide assay: method evaluation and reference intervals in adults and children
Morovat, Alireza; Catchpole, Anthony; MEURISSE, Angélique ULiege et al

in Clinical Chemistry & Laboratory Medicine (2013), 51(10), 2009-2018

Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen- I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference ... [more ▼]

Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen- I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference intervals for PINP in both adults and children. Methods: Assay imprecision, recovery and interference were studied. Serum and plasma values were compared, and PINP stability was assessed. Using 828 specimens, IDS iSYS intact PINP and Roche E170 total PINP values were compared. Specimens from 597 adults and 485 children and adolescents were used to establish reference intervals for intact PINP. Results: The method demonstrated good recovery and acceptable imprecision. The assay was unaffected by icterus and lipaemia, but haemolysis decreased measured PINP. Serum and plasma values were comparable. There was a non-linear relation between IDS intact and Roche total PINP values. Pre- and post-menopausal women had comparable PINP values, but there was a difference between women of different age groups. Serum PINP in men showed a decline in young age up to 45 years, but remained steady thereafter. Separate reference intervals were established for four age groups in women and for two age groups in men. Data for children were partitioned into four-year age groups, and these showed PINP to be high with no major gender differences until 12 years of age. Thereafter, values in females decreased in 13–16 years age groups and further in 17–20 years age groups, whereas PINP increased in boys of 13–16 years of age with a subsequent decline at 17–20 years. Conclusions: The IDS iSYS PINP intact assay appears to be reliable. We have established gender- and age-related reference intervals for children and adults based on a relatively large healthy North European population. [less ▲]

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See detailTechnical and clinical evaluation of the VITROS Immunodiagnostic Products 25-OH Vitamin D Total Assay - comparison with marketed automated immunoassays and a liquid chromatography - tandem mass spectrometry method
CAVALIER, Etienne ULiege; ROUSSELLE, Olivier ULiege; FERRANTE, Nunzio ULiege et al

in Clinical Chemistry & Laboratory Medicine (2013), 51(10), 1983-1989

Background: The study was conducted to evaluate the technical and clinical performance of the VITROS ® Immunodiagnostic Products 25-OH Vitamin D Total Assay, and compare it with the performance of five ... [more ▼]

Background: The study was conducted to evaluate the technical and clinical performance of the VITROS ® Immunodiagnostic Products 25-OH Vitamin D Total Assay, and compare it with the performance of five marketed automated assays and a liquid chromatography/mass spectrometry reference method (LC-MS/MS). Methods: Three hundred patient serum samples were used to compare the correlation of the VITROS ® 25-OH Vitamin D Total Assay with both the other immunoassays and the LC-MS/MS method, using Passing-Bablok regression and Bland-Altman analyses. Concordance of the diagnosis of vitamin D status was calculated to test the agreement between the different assays. In addition, samples containing vitamin D2 were used to test the assay ’ s ability to detect the D2 form of the vitamin. Results and conclusions: These results from the VITROS ® 5-OH Vitamin D Total Assay generally correlated well with those from most of the marketed immunoassays. Cross-reactivity of the D2 form was calculated as being close to 100%. Additionally, we found substantial variability in performance amongst the various assays, which suggests the need for optimisation and recalibration of commercial methods. [less ▲]

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See detailThe diagnostic utility of folate receptor autoantibodies in blood
Sequeira, JM; RAMAEKERS, Vincent ULiege; Quadros, EV

in Clinical Chemistry & Laboratory Medicine (2013), 51(3), 545-54

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See detailClinical recognition and aspects of the cerebral folate deficiency syndromes
RAMAEKERS, Vincent ULiege; Sequeira, JM; Quadros, EV

in Clinical Chemistry & Laboratory Medicine (2013), 51(3), 497-511

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See detailEvaluation of the cross-reactivity of 25-hydroxyvitamin D2 on seven commercial immunoassays on native samples
LE GOFF, Caroline ULiege; PEETERS, Stéphanie ULiege; CRINE, Yannick ULiege et al

in Clinical Chemistry & Laboratory Medicine (2012), 50(11), 2031-2

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See detailAberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report
CAVALIER, Etienne ULiege

in Clinical Chemistry & Laboratory Medicine (2012), 50(3), 583-584

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See detailElevation of cardiac and oxidative stress biomarkers after a running activity in sedentary subjects
LE GOFF, Caroline ULiege; Bury, Thierry ULiege; Rodriguez de la Cruz, Carlos ULiege et al

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(Special Suppl), 313

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See detailFatty acids and cardiovascular risk
LE GOFF, Caroline ULiege; Leroy, Ludovic; Kaux, Jean-François ULiege et al

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(Special Suppl), 313

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See detailHuman anti-animal antibodies: even today, it is still a problem in routine practice: illustration with 4 clinical cases.
CAVALIER, Etienne ULiege; CARLISI, Ignazia ULiege; DELANAYE, Pierre ULiege

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(s1), 409

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See detailVitamin D: clinical relevance, analytical issues
CAVALIER, Etienne ULiege

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(s1), 66

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See detailCystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies
Bargnoux, A. S.; Cavalier, Etienne ULiege; Cristol, J. P. et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 265-70

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human ... [more ▼]

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance of this new assay was evaluated. Clinical relevance was determined by comparison with a reference method in a cohort of kidney transplant patients. Patients and methods: First, the analytical performance of the Binding Site cystatin C kit was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy. Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in our population of renal transplantation. Conclusions: The use of algorithms based on cystatin C and creatinine could provide a reliable estimate of GFR in kidney transplantation. [less ▲]

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See detailValidation of the Abbott Architect 25(OH)-vitamin D assay
CAVALIER, Etienne ULiege; CARLISI, Ignazia ULiege; BEKAERT, Anne-Catherine ULiege et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(s1), 418

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See detailAnalytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)
Cavalier, Etienne ULiege; Carlisi, Ignazia ULiege; Bekaert, Anne-Catherine ULiege et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 271-275

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See detailAnalytical validation of the BAP OSTASE on Liaison (DiaSorin).
Cavalier, Etienne ULiege; Rozet, Eric ULiege; Carlisi, Ignazia ULiege et al

in Clinical Chemistry & Laboratory Medicine (2010), 48(1), 67-72

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See detailA new statistical method for evaluating long-term analytical performance of laboratories applied to an external quality assessment scheme for flow cytometry.
Coucke, Wim; Van Blerk, Marjan; Libeer, Jean*-Claude et al

in Clinical Chemistry & Laboratory Medicine (2010), 48(5), 645-50

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the ... [more ▼]

BACKGROUND: The Belgian External Quality Assessment Scheme for Flow Cytometry evaluates the long-term analytical performance of participating laboratories by calculating a regression line between the target and reported values of each parameter for each laboratory during the past 3 years. This study aims to develop a method to find laboratories with aberrant variability or bias using robust techniques and to obtain robust estimates of the variability. METHODS: A method is proposed to find outliers with respect to the individual regression line, followed by a step to find regression lines with excessive variability and finally a step to find regression lines with high bias. RESULTS: The model was applied to the results obtained by 52 laboratories for CD4%. From the 1340 data points, 35 were determined to be regression outliers. The second step revealed one regression line with excessive variability; the third step detected three regression lines with exceeding bias. CONCLUSIONS: The methodology allows assessment of the long-term performance of laboratories, taking into account samples with different target values. Outliers in the first step indicate accidental mistakes, outliers in the second and third step point to high analytical variability or bias. [less ▲]

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See detailInterpretation of serum parathyroid hormone concentrations in dialysis patients: what do the KDIGO guidelines change for the clinical laboratory?
Souberbielle, Jean-Claude; Cavalier, Etienne ULiege; Jean, Guillaume

in Clinical Chemistry & Laboratory Medicine (2010), 48(6), 769-774

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See detailMulticentre evaluation of the Tosoh HbA1c G8 analyser
Chapelle, Jean-Paul ULiege; Teixeira, Jelda ULiege; Maisin, Diane et al

in Clinical Chemistry & Laboratory Medicine (2010), 48(3), 365-371

Detailed reference viewed: 85 (3 ULiège)