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See detailINOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHY
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, D. et al

in World Rabbit Science (2009), 17(4), 185-193

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼]

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲]

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See detailExperimentally induced epizootic rabbit enteropathy: Clinical, histopathological, ultrastructural, bacteriological and haematological findings
Dewrée, Roxane; Meulemans, L.; Lassence, Cédric ULg et al

in World Rabbit Science (2007), 15(2, APR-JUN), 91-102

Epizootic rabbit enteropathy is an emerging disease that has appeared in French intensive enclosed rabbit farms since the beginning of 1997. Common clinical signs are mild watery diarrhoea with ... [more ▼]

Epizootic rabbit enteropathy is an emerging disease that has appeared in French intensive enclosed rabbit farms since the beginning of 1997. Common clinical signs are mild watery diarrhoea with considerable distension of the abdomen. At necropsy, a significant dilation of the stomach and small intestine without gross evidence of acute or chronic enteric lesions (inflammation or congestion) was observed. The purpose of this study was to describe the anatomopathologic changes concerning the small intestine and those concerning the blood profile, in experimentally infected rabbits. In a first part of the experiment, thirty animals were inoculated with a reference inoculum and five were kept as controls for clinical signs examination and histopathological study. In a second part, 17 out of the inoculated rabbits and the 5 controls animals were randomly assigned to blood testing. Microscopic lesions were studied in sections from the different parts of the small intestine (duodenum, jejunum and ileum) by light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The histological findings revealed only limited inflammation in inoculated animals. Major villous changes were atrophy, fusion, destruction and loss of epithelial cells. In inoculated rabbits, the congestion and dilation of blood vessels of jejunal lamina propria were significantly higher than in control animals (P<0.005). There was significantly more (P<0.05) apoptosis of cells of the jejunal epithelium in inoculated rabbits than in control animals. Infiltration of polymorphonuclear neutrophiles was observed into the jejunal or ileal tunica muscularis. SEM performed on the intestinal tract of 15 inoculated rabbits revealed blankets and globular particles of mucus associated with numerous bacteria on jejunum and ileum villi. This was; not observed in the intestinal tract of control rabbits. Bacteria were found adhering to the epithelial surface and inside intestinal epithelial cells in a few animals by TEM and by light microscopy after Warthin-Starry staining. None of the bacteria isolated from the intestinal mixed contents and cultivated on usual media, are commonly known as rabbit's pathogens. Regarding the haernatological profile, neutrophil counts significantly increased (P<0.05) and lymphocyte counts significantly decreased (P<0.01), in inoculated rabbits compared to those of the control group. [less ▲]

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See detailVariations in plasma matrix metalloproteinases and placental pregnancy-associated glycoproteins during gestation in rabbits (Oryctolagus cuniculus)
Othmani-Mecif, Khira; Benazzoug, Y.; Jacob, M. P. et al

in World Rabbit Science (2006), 14(1, JAN-MAR), 39-50

Adaptation to pregnancy involves major maternal anatomical, physiological and metabolic modifications to support the mother's metabolic needs and those of the growing foetus. Invasion of embryonic ... [more ▼]

Adaptation to pregnancy involves major maternal anatomical, physiological and metabolic modifications to support the mother's metabolic needs and those of the growing foetus. Invasion of embryonic territories and implantation in humans and other mammals is the result of an active biochemical process. The invasive faculty of cytotrophoblastic cells depends on their capacity to secrete proteolytic enzymes such as matrix metalloproteinases (MMPs). Many studies have addressed the variations in MMPs in placental tissues, but few have reported on their measurements in plasma. Furthermore, the Pregnancy-Associated Glycoproteins (PAGs), which are synthesized in the syncytiotrophoblast and used as early markers of pregnancy in domestic and wild animals, have not yet been studied in rabbits. In this study, we attempt to purify PAGs from rabbit placenta using a previously described methodology and to measure their concentrations as well as those of MMP-2 and MMP-9 in the plasma throughout pregnancy. In the course of the work, we noted certain modifications of the biochemical parameters in plasma during gestation, essentially in glycemia and lipemia. We detected proteins immunologically-related to PAGs in placental extracts between D-14 to D-21. Until now, it has been impossible to develop a homologous RIA in order to measure PAGs in rabbit plasma, as the recovery of PAGs during the purification procedure was very low and PAGs were undetectable in rabbit plasma using heterologous RIAs. Finally, we demonstrated a rise in MMP-2 and -9 at the middle and the end of the gestation. However, the small variations presented by these matrix metalloproteinases preclude the use of plasma levels of these enzymes as early markers of pregnancy progress in the rabbit. [less ▲]

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See detailVirus infections of rabbits
Marlier, Didier ULg; Bertagnoli, Stéphane

in World Rabbit Science (2000, July 04), 8(supplément 1),

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See detailProduction of anti-PMSG antibodies and its relation to the productivity of rabbit does
Lebas, F.; Theau-Clement, M.; Remy, Benoît ULg et al

in World Rabbit Science (1996), 4(2), 57-62

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