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See detailThe A2 gene of alcelaphine herpesvirus-1 is a transcriptional regulator affecting cytotoxicity in virus-infected T cells but is not required for malignant catarrhal fever induction in rabbits.
Parameswaran, Nevi; Dewals, Benjamin G ULg; Giles, Tom C. et al

in Virus research (2014)

Alcelaphine herpesvirus-1 (AlHV-1) causes malignant catarrhal fever (MCF). The A2 gene of AlHV-1 is a member of the bZIP transcription factor family. We wished to determine whether A2 is a virulence gene ... [more ▼]

Alcelaphine herpesvirus-1 (AlHV-1) causes malignant catarrhal fever (MCF). The A2 gene of AlHV-1 is a member of the bZIP transcription factor family. We wished to determine whether A2 is a virulence gene or not and whether it is involved in pathogenesis by interference with host transcription pathways. An A2 gene knockout (A2DeltaAlHV-1) virus, revertant (A2revAlHV-1) virus, and wild-type virus (wtAlHV-1) were used to infect three groups of rabbits. A2DeltaAlHV-1-infected rabbits succumbed to MCF, albeit with a delayed onset compared to the control groups, so A2 is not a critical virulence factor. Differential gene transcription analysis by RNAseq and qRT-PCR validation of a selection of these was performed in infected large granular lymphocyte (LGL) T cells obtained in culture from the MCF-affected animals. A2 was involved in the transcriptional regulation of immunological, cell cycle and apoptosis pathways. In particular, there was a bias towards gammadelta T cell receptor (TCR) expression and downregulation of alphabeta TCR. TCR signalling, apoptosis, cell cycle, IFN-gamma and NFAT pathways were affected. Of particular interest was partial inhibition of the cytotoxicity-associated pathways involving perforin and the granzymes A and B in the A2DeltaAlHV-1-infected LGLs compared to controls. In functional assays, A2DeltaAlHV-1-infected LGLs were significantly less cytotoxic than wtAlHV-1- and A2revAlHV-1-infected LGLs using rabbit corneal epithelial cells (SIRC) as targets. This implies that A2 is involved in a pathway enhancing the expression of LGL cytotoxicity. This is important as virus-infected T cell cytotoxicity in vivo has been suggested as a potential mechanism of disease induction in MCF. [less ▲]

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See detailCurrent impact and future directions of high throughput sequencing in plant virus diagnostics.
Massart, Sébastien ULg; Olmos, Antonio; Jijakli, Haissam ULg et al

in Virus Research (2014), 188

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of ... [more ▼]

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of highthroughput sequencing (also called Next Generation Sequencing – NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed. [less ▲]

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See detailCervid herpesvirus 2 experimentally reactivated in reindeer can produce generalized viremia and abortion
das Neves, C.; Mork, T.; Thiry, Julien ULg et al

in Virus Research (2009), 145

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See detailCharacterization of caprine herpesvirus 1 glycoprotein D gene and its translation product
Keuser, Véronique; Detry, Bruno; Thiry, Julien ULg et al

in Virus Research (2006), 115(2), 112-121

Caprine herpesvirus 1 (CpHV- 1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 ... [more ▼]

Caprine herpesvirus 1 (CpHV- 1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 (BoHV-1) which causes infectious bovine rhinotracheitis. Glycoprotein D (gD) mediates important functions in alphaherpesviruses and is also a main inummogen. The sequence of CpHV-1 gD gene and the biochemical properties of its translation product were analyzed and compared to those of BoHV-1 and other alphaherpesviruses. A relatively high homology was found between CpHV-1 and BoHV-1 glycoproteins D amino acid sequences (similarity of 68.8%). Moreover, six cysteine residues are conserved by CpHV-1 gD and the other studied alphaherpesviruses. CpHV-1 gD has a molecular mass similar to BoHV-1 gD and contains complex N-linked oligosaccharides. In contrast to the BoHV-1 gD, CpHV-1 gD is expressed as a late protein. In spite of the observed differences which could influence its biological functions, CpHV-1 gD, shares most characteristics with other alphaherpesviruses and especially BoHV-1. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailOncogenic potential of Tax requires phosphorylation on serines 106 et 293.
Willems, Luc ULg; Grimonpont, Catherine; Kerkhofs, Pierre et al

in Virus Research (1997), 47(2 -Special Issue), 121-122

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See detailMutations in the immunosuppressive peptide of bovine leukema virus affect fusion and infectivity in vivo
Gatot, Jean-Stéphane; Kettmann, Richard ULg; Callebaut-Mornon, Isabelle et al

in Virus Research (1997), 47(2-Special Issue), 103

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See detailEvidence For The Assignment Of Two Strains Of Splv To The Genus Potyvirus Based On Coat Protein And 3' Non-Coding Region Sequence Data
Colinet, Dominique; Kummert, Jean; Lepoivre, Philippe ULg

in Virus Research (1997), 49(1), 91-100

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