References of "Parasitology"
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See detailDoes the acanthocephalan parasite Polymorphus minutus modify the energy reserves and antitoxic defences of its intermediate host Gammarus roeseli?
Gismondi, Eric ULg; Cossu-Leguille, Carole; Beisel, Jean-Nicolas

in Parasitology (2012), 139

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See detailIntestinal Growth and Pathology og Giardia duodenalis assemblage subtype AI, AII, B end E in the gerbil model
Bénéré, Ely; Van Assche, Tim; Van Ginneken, Chris et al

in Parasitology (2012), 139

This study investigated the significance of the genetic differences between assemblages A, B and E on intestinal growth and virulence. Intestinal growth and virulence were studied in 2 laboratory (AI: WB ... [more ▼]

This study investigated the significance of the genetic differences between assemblages A, B and E on intestinal growth and virulence. Intestinal growth and virulence were studied in 2 laboratory (AI: WB and B: GS/M-83-H7) and 6 field isolates of assemblage subtype AI, AII, B and EIII. Intestinal trophozoite burdens, body weight and faecal consistency were monitored until day 29 post-infection (p.i.), morphological (mucosal architecture and inflammation) and functional (disaccharidase and alkaline phosphatase enzyme activity) damage to the small intestine were evaluated on days 7 and 18 p.i. The assemblage subtypes AI and B were more infectious and produced higher trophozoite loads for a longer period compared to the subtypes AII and EIII. The body weight of infected gerbils was significantly reduced compared to uninfected controls, but did not differ between the assemblage subtypes. Consistent softening of the faeces was only observed with assemblage B. Assemblage B next to assemblage subtype AI elicited relatively higher pathogenicity, characterized by more extensive damage to mucosal architecture, decreased brush-border enzyme function and infiltration of inflammatory cells. Assemblage EIII and AII isolates showed relatively low virulence. The Giardia assemblage subtypes exhibit different levels of growth and virulence in the gerbil model. [less ▲]

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See detailIn vitro titration of Theileria parva tick derived stabilates
Marcotty, T.; Speybroeck, N.; Berkvens, D. et al

in Parasitology (2004), 128(Part 2), 131-137

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic ... [more ▼]

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. Parva sporozites used in this method is usually determined by in vitro titration. An alternative in vivo method of quantitification of sporozoites ill whole tick-derived stabilites is proposed. The method consists of incubating serially diluted T. Parva stabilities with boville peripheral blood lymphocytes, the host cell that is infected naturally. Allowing the cultures to incubate undisturbed for the full cultivation period (10 days) reduced the variability amoung replicate titrations. fungal contaminations were avoided by centrifuging stabilates at 400 g prior to the incubation, which did not precipiate sporozoites significantly. Fungistics, Nysatin and Flucytosine did not appear to interfere with the in vitro development of 2 stabilates but their effect on fungal growth was limited. In vitro titration data were compared to in vivo infection data for 2. In vivo titration of T. parva sporozoites should allow more ethicl and efficient research on the preparation and storage of T. Parva tick-derived stabilates. [less ▲]

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