References of "Nucleic Acids Symposium Series"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailStudies of sequence specific recognition and interaction of bis-hairpin polyamide minor groove binders with target DNA duplexes
Boutorine, A. S.; Buchmann, W.; Halby, L. et al

in Nucleic Acids Symposium Series (2008), 52

Study of the binding of bis-MGB using DNA footprinting, native gel shift, thermal denaturation, mass spectrometry and circular dichroism. Elaboration of a method to determin

Detailed reference viewed: 24 (2 ULg)
Full Text
Peer Reviewed
See detailRecognition of homopyrimidine mismatches by distance-constrained macrocyclic bisintercalators
Bahr, Matthias; Gabelica, Valérie ULg; Granzhan, Anton et al

in Nucleic Acids Symposium Series (2008), 52

Binding of three macrocyclic bisintercalators to mismatch-containing duplexes was analyzed by thermal denaturation experiments, electrospray mass spectrometry studies (ESI-MS) and fluorescent intercalator ... [more ▼]

Binding of three macrocyclic bisintercalators to mismatch-containing duplexes was analyzed by thermal denaturation experiments, electrospray mass spectrometry studies (ESI-MS) and fluorescent intercalator displacement (FID) titrations. The macrocyclic bisintercalators bind to duplexes containing mismatched thymine bases with high selectivity over the fully matched one and affinity in the submicromolar range (Kd). The FID results also demonstrate that the macrocyclic naphthalene derivative BisNP preferentially binds to pyrimidine-pyrimidine mismatches compared to all other possible base mismatches. This ligand also efficiently competes with a DNA enzyme (M.TaqI) for binding to a duplex with a TT-mismatch [less ▲]

Detailed reference viewed: 27 (0 ULg)
Full Text
Peer Reviewed
See detailLigand binding to tetra-end-linked (TGGGGT)4 G-quadruplexes: an electrospray mass spectroscopy study
Amato, Jussara; Oliviero, Giorgia; Borbone, Nicola et al

in Nucleic Acids Symposium Series (2008), 52

The binding properties of a series of known G-quadruplex ligands have been studied by ESI-MS experiments. The tetramolecular (TG(4)T)(4) quadruplex and its analogues I and II blocked, respectively, at the ... [more ▼]

The binding properties of a series of known G-quadruplex ligands have been studied by ESI-MS experiments. The tetramolecular (TG(4)T)(4) quadruplex and its analogues I and II blocked, respectively, at the 3' or 5'-end by a tetra-end-linker (TEL) unit were chosen as the ligands targets. The stoichiometries of the obtained complexes as well as the ligand affinity and selectivity to the different quadruplexes were determined to deduce the ligand binding site. The TEL derivatives I and II allowed the probing of the grooves contribution to the binding of ligands to G-quadruplexes, demonstrating that the 3' and 5' quartets are not equivalent binding sites for ligand end-stacking. [less ▲]

Detailed reference viewed: 92 (7 ULg)
Full Text
Peer Reviewed
See detailA short C-rich PNA fragment capable to form novel G-quadruplex-PNA complexes
Amato, Jussara; Gabelica, Valérie ULg; Borbone, Nicola et al

in Nucleic Acids Symposium Series (2008), 52

In this work we investigated the interaction between the short ac(4)a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different ... [more ▼]

In this work we investigated the interaction between the short ac(4)a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different folding topologies. The T(G(4)T)(3)G(4)T and the recently reported tetra-end-linked-(TG(4)T)(4) G-rich oligonucleotides (GROs) were chosen and synthesized for this study. UV, CD, and MS experiments revealed the formation of novel 1:1 G-quadruplex-PNA complexes besides the expected DNA-PNA heteroduplexes [less ▲]

Detailed reference viewed: 55 (7 ULg)
Peer Reviewed
See detailThe formation and enzymatic repair of DNA modifications caused by haloethylnitrosoureas and related compounds
Ludlum, D.; Habraken, Yvette ULg; Carter, C. et al

in Nucleic Acids Symposium Series (1992), 105

Detailed reference viewed: 3 (0 ULg)