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See detailPattern of morpho-functional diversifcation of damselfishes (Pomacentridae)
Frederich, Bruno ULg

Conference (2013, December 18)

Coral reef fishes represent one of the most outstandingly diverse assemblages of vertebrates on the planet but our understanding of their mode of diversification remains limited. Currently, some ... [more ▼]

Coral reef fishes represent one of the most outstandingly diverse assemblages of vertebrates on the planet but our understanding of their mode of diversification remains limited. Currently, some biologists are testing various hypotheses about the evolutionary history of coral reef fishes and are exploring the factors driving their diversification. During my post-doctoral research, I explored the pattern of morphological diversification of damselfishes (Pomacentridae, 386 species). I produced a time-calibrated phylogeny based on 8 loci including 208 species and collected eco-morphological data (trophic data, body shape and oral jaws shape) in more than 120 species. Using various phylogenetic comparative methods, I have illusrated that the Pomacentridae observed repeated ecological radiation and morphological convergence during their evolutionary history. I have also highlighted the primary role of a ligament joining the mandible and the hyoid in the evolution and the morpho-functional diversification of pomacentrids. [less ▲]

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See detaillutte contre la mortalité périnatale: actualisation de l'asphyxie périnatale
Battisti, Oreste ULg

Conference (2013, December 18)

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See detailStructural and biochemical study of the proteins AmiC, NlpD and FtsW involved in the bacterial cell division
Rocaboy, Mathieu ULg

Doctoral thesis (2013)

Cell division in the Gram negative bacterium Escherichia coli is a highly coordinated mechanism involving various physiological functions such as chromosome segregation, cell envelope invagination ... [more ▼]

Cell division in the Gram negative bacterium Escherichia coli is a highly coordinated mechanism involving various physiological functions such as chromosome segregation, cell envelope invagination, peptidoglycan synthesis at the division site and separation of the daughter cells. All these functions require a high level of spatio-temporal regulation in order to preserve the physical integrity of the cell. At least 20 proteins required for a proper cell division are recruited to the division site to form a supramolecular complex called the divisome. This thesis work focused on three major components of the E. coli division machinery: the N-acetylmuramyl L-alanine amidase AmiC, the LytM factor NlpD and the lipid II flippase FtsW. These proteins are recruited at midcell at a late stage of cell division. FtsW is an integral membrane protein crucial for the translocation of the peptidoglycan precursor from the cytoplasm to the periplasm where it will be processed to produce septal peptidoglycan. AmiC acts as a septal peptidoglycan hydrolase that allow the separation of the daughter cells. This enzyme has been shown to be activated by the LytM factor NlpD. The crystal structure of AmiC from E. coli presented in this work confirms the presence of an inhibitory helix in the active site. The AmiC variant lacking this helix exhibits by itself an activity comparable to that of the wild type AmiC activated by NlpD. Furthermore, the direct interaction between AmiC and NlpD has been detected by microscale thermophoresis with an apparent Kd of about 13 µM. The crystal structure of AmiC also reveals the β-sandwich fold of the AMIN domain, responsible for the septal targeting of AmiC to the division site. The two symmetrical four-stranded β-sheets exhibit highly conserved motifs on the two outer faces. Along with the peptidoglycan binding capacity of the AMIN domain, results obtained so far suggest that the AMIN domain could be involved in the recognition of a specific peptidoglycan architecture or a composition different than the lateral peptidoglycan. Production screenings of FtsW from different strains were realized and FstW from E. coli was purified. This challenging project will require additional efforts to obtain sufficient amount of protein for structural investigation. Information gathered in this work confirms the high level of regulation of the hydrolytic activity at the septum and gives a structural basis for a more precise molecular characterization of the division site targeting. Disruption or over-activation of these regulation mechanisms could represent a new strategy in the development of antibacterial compounds. [less ▲]

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See detailRecycling and sustainable development
Courard, Luc ULg

Scientific conference (2013, December 17)

Conditions for developing recycling in good conditions (environmental, economical and social)

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See detailDéfinition de propriétés acido-basiques dans les liquides ioniques de seconde génération
Robert, Thierry ULg

Doctoral thesis (2013)

The ionic liquids are salts having the particularity of being molten at temperatures lower than 100 °C. Consequently, these new solvents mainly made up of ions have original physicochemical properties ... [more ▼]

The ionic liquids are salts having the particularity of being molten at temperatures lower than 100 °C. Consequently, these new solvents mainly made up of ions have original physicochemical properties. Within a few years, the ionic liquids passed from a laboratory curiosity to a true field of research impossible to circumvent, currently in full rise. Indeed, the replacement of usual organic solvents in catalytic and/or separation processes by these neoteric solvents offers many advantages but also new opportunities for the “Green Chemistry”. However, the systematic exploitation of the ionic liquids as reactional media rests in particular on the understanding of their chemical properties, which for some of them are still scarcely known, such as the acidity for example. This thesis thus aims to undertake a study of the acido-basic properties of (and in) these solvents, and more particularly to determine the accessible levels of acidity for acid solutions (with added HOTf or HNTf2) in second generation ionic liquids such as [HNEt3][NTf2], [BMIm][NTf2], [BHIm][NTf2], [BMIm][BF4], [BMIm][OTf], [BMIm][PF6] and [BMIm][SbF6]. In order to evaluate these acidity levels, we propose two different methods, each one resting on an extra-thermodynamic assumption. The first, the Hammett acidity function H0, is based on the protonation equilibrium of indicators whose pKa's are proposed as solvent independent. The second, the Strehlow potentiometric function R0(H+), consists in measuring, in a given solvent, the electrochemical potential of the proton compared to the ferricinium/ferrocene redox couple whose potential is supposed to be independent of the solvent, and then to refer it versus the Normal Hydrogen Electrode (NHE) in water. The two methods lead to the same conclusions. Firstly, the ionic liquids are generally contaminated by residual basic impurities (from solvents needed for the synthesis…) which need to be neutralized before reaching the acidity characteristic of the medium. The levels of acidity then obtained are very high and can reach values as high as R00(H+) = -10 in the case of [BMIm][BF4]. Then, the accessible level of acidity in an ionic liquid depends mainly on the nature of its anion, and not of that of its cation. We thus obtain the following classification, by decreasing acidity: [PF6-] > [BF4-] > [NTf2-] > [OTf-], indicating that the triflate is the more solvating anion It was found however that the Hammett acidity function led, for the same concentration in acid, to different levels of acidity, depending on the indicator used. The ionic liquids would consequently be media less dissociating than estimated in the literature and the Hammett function would then be related to an apparent acidity (H0)app, underestimating the real acidity. Finally, a difference in acidity between HNTf2 and HOTf is observed in [BMIm][NTf2] and [BMIm][OTf], HNTf2 showing an acidic character stronger than HOTf. On the other hand, in [BMIm][OTf] these two acids show the same acidity since that of HNTf2 has been leveled by solvent. [less ▲]

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See detailAnalyse d’un dispositif de (re)mise en emploi : Le cas d’un bureau de recrutement, recherche et sélection de personnel
Gérard, Julie ULg

Conference (2013, December 17)

Au cours de ces dernières années, le souci d’allier flexibilité et sécurité au travail a conduit à l’essor considérable des métiers de l’intermédiation (Orianne et Maroy, 2008). Dans le domaine de ... [more ▼]

Au cours de ces dernières années, le souci d’allier flexibilité et sécurité au travail a conduit à l’essor considérable des métiers de l’intermédiation (Orianne et Maroy, 2008). Dans le domaine de l’accompagnement et du développement de carrière, divers dispositifs privés d’aide et de suivi ont vu le jour afin de contribuer à l’amélioration des conditions de travail et de guider les travailleurs dans leurs transitions professionnelles. Par leur action, ces nouveaux dispositifs – que nous qualifierons de dispositifs d’intermédiation – semblent complexifier les temporalités sociales en créant de nouvelles opportunités d’emploi et/ou en redéfinissant les rythmes de carrière. À l’aide d’une étude de cas réalisée dans le secteur du recrutement belge, nous analyserons la capacité d’un processus de (re)mise en emploi à remplir de tels objectifs dans un contexte de gestion mixte du marché du travail. À partir d’une approche qualitative cumulant des entretiens semi-directifs et de l’observation participante, nous montrerons qu’en plus de régir l’offre et la demande de travail, ce dispositif d’intermédiation, par son système de cooptation interpersonnelle et d’accompagnement de carrière, assure la fluidification du marché et sécurise les transitions professionnelles. [less ▲]

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See detailAurorae at Jupiter: a selection of recent results
Bonfond, Bertrand ULg

Scientific conference (2013, December 17)

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See detailAnalyse du métier de facilitateur et de son rôle dans la diffusion de normes d’emploi : le cas d’un bureau de chômage belge
Beuker, Laura ULg

Conference (2013, December 17)

En 2004, suite aux injonctions européennes en matière d’activation impulsées par les lignes directrices de 2003-2005, la Belgique met en place le PAC (ou Plan d’Accompagnement des chômeurs). Cette ... [more ▼]

En 2004, suite aux injonctions européennes en matière d’activation impulsées par les lignes directrices de 2003-2005, la Belgique met en place le PAC (ou Plan d’Accompagnement des chômeurs). Cette importante réforme prévoit – notamment – la mise en place de la procédure d’activation du comportement de recherche d’emploi par l’Office National de l’Emploi (Ou l’ONEm). Désormais, des agents sont spécifiquement dédiés à l’évaluation des efforts menés par les demandeurs d’emploi (ou DE) pour s’insérer sur le marché du travail : ce sont les facilitateurs. Si la procédure d’évaluation est officiellement définie, la décision, quant à elle, est bel et bien fonction de l’agent, qui doit interpréter le cadre réglementaire général à l’aune de la situation singulière du demandeur d’emploi. À l’aide d’une étude de cas réalisée au sein d’un bureau de chômage situé en Belgique francophone, nous allons tenter d’ouvrir la « boîte noire » du jugement des facilitateurs. À partir d’une approche qualitative cumulant des entretiens semi-directifs et de l’observation d’interactions entre facilitateurs et demandeurs d’emploi, nous montrerons que la décision des facilitateurs n’est ni arbitraire, ni déterminée. Elle est, au contraire, le fruit de la mobilisation d’une multitude d’appuis conventionnels dans l’action (Dodier, 1993). En outre, dans la lignée des travaux de Benarrosh (2000 ; 2006 ; 2012) nous démontrerons que ces agents sont des « pourvoyeurs » de normes institutionnelles précises à destination des demandeurs d’emploi. [less ▲]

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See detailL'ENMG, c'est le pied !
WANG, François-Charles ULg

Conference (2013, December 17)

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See detailLa photographie et l'extériorité de l'oeuvre d'art dans les écrits de Kracauer
Barbion, Sébastien ULg

Scientific conference (2013, December 16)

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See detailPrésentation du film "It's a Wonderful Life" de Frank Capra
Tomasovic, Dick ULg

Conference given outside the academic context (2013)

Présentation du contexte de création et de production du film "La vie est belle" de F. Capra.

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See detailEtude du rôle des modalités de communication dans la construction et le maintien du common ground. Développement d'une méthodologie d'analyse.
Defays, Aurore ULg; Giboin, Alain

Scientific conference (2013, December 16)

This communication aims to present the methodology developed to better understand the role of communication modalities in the process for grouding. We show that each modality of communication has its own ... [more ▼]

This communication aims to present the methodology developed to better understand the role of communication modalities in the process for grouding. We show that each modality of communication has its own role in the grounding process (analyzed with a coding scheme). We also show that in situations of remote work, communication modalities are mobilized differently by partners. Moreover, in case of impossibility to mobilize one particular modality, some compensation strategies are observed. This oral presentation also highlights joint working between Aurore Defays and Alain Giboin during a research stay in Wimmics team in Sophia Antipolis-Méditerranée Centre. [less ▲]

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See detailSémiotique de la communication en co-présence et à distance. Du textualisme à la sémiotique des pratiques
Dondero, Maria Giulia ULg; Angenot, Valérie ULg; Joachim, Guillaume ULg et al

in Interfaces Numériques (2013), 2(3), 531-567

This article aims to challenge and reflect on the theories issued by both textualist semiotics and the semiotics of practices. Our reflexion shall focus, on the one hand, on synchronic communication in ... [more ▼]

This article aims to challenge and reflect on the theories issued by both textualist semiotics and the semiotics of practices. Our reflexion shall focus, on the one hand, on synchronic communication in copresence and, on the other hand, on virtual participative remote collaboration by means of digital interfaces. In high-level collaborative activities —such as remote architectural project developments— the practices of the partners involved in the communication process are conditioned by intrinsic material constraints; they have to gradually readapt to the constantly renewed dynamics of the predicative structures in order to ensure communication efficiency. One of the main purposes of this paper consists in exploring the theoretical propositions and methodologies connected to practices, as proposed by Jacques Fontanille’s semiotics (2008) and by Jacques Theureau’s ergonomics of the “course of action” (2006). The results of these theoretical reflections will be tested through their application to a specific corpus, in an aim to confront the efficiency of such proposals with a case study. Multimodality and the role played by the different types of utterances in the efficiency of communication, as well as the adaptation of the semiotic practices to the digital interfaces also fall under the scope of this paper. Besides, the authors propose a reflection on potential mediations between the ongoing practices and their objectivation through textualisation and notation. [less ▲]

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See detailArbitrary Lagrangian Eulerian simulations of stationary and non-stationary metal forming processes
Boman, Romain ULg; Ponthot, Jean-Philippe ULg

in AIP Conference Proceedings (2013, December 16), 1567

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See detailOn the Encoding of Proteins for Disordered Regions Prediction
Becker, Julien ULg; Maes, Francis; Wehenkel, Louis ULg

in PLoS ONE (2013)

Disordered regions, i.e., regions of proteins that do not adopt a stable three-dimensional structure, have been shown to play various and critical roles in many biological processes. Predicting and ... [more ▼]

Disordered regions, i.e., regions of proteins that do not adopt a stable three-dimensional structure, have been shown to play various and critical roles in many biological processes. Predicting and understanding their formation is therefore a key sub-problem of protein structure and function inference. A wide range of machine learning approaches have been developed to automatically predict disordered regions of proteins. One key factor of the success of these methods is the way in which protein information is encoded into features. Recently, we have proposed a systematic methodology to study the relevance of various feature encodings in the context of disulfide connectivity pattern prediction. In the present paper, we adapt this methodology to the problem of predicting disordered regions and assess it on proteins from the 10th CASP competition, as well as on a very large subset of proteins extracted from PDB. Our results, obtained with ensembles of extremely randomized trees, highlight a novel feature function encoding the proximity of residues according to their accessibility to the solvent, which is playing the second most important role in the prediction of disordered regions, just after evolutionary information. Furthermore, even though our approach treats each residue independently, our results are very competitive in terms of accuracy with respect to the state-of-the-art. A web-application is available at http://m24.giga.ulg.ac.be:81/x3Disorder. [less ▲]

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See detailA new methodological approach for error distributions selection
Hambuckers, julien ULg; Heuchenne, Cédric ULg

Conference (2013, December 15)

Since 2008 and its financial crisis, an increasing attention has been devoted to the selection of an adequate error distribution in risk models, in particular for Value-at-Risk (VaR) predictions. We ... [more ▼]

Since 2008 and its financial crisis, an increasing attention has been devoted to the selection of an adequate error distribution in risk models, in particular for Value-at-Risk (VaR) predictions. We propose a robust methodology to select the most appropriate error distribution candidate, in a classical multiplicative heteroscedastic model. In a first step, unlike to the traditional approach, we do not use any GARCH-type estimation of the conditional variance. Instead, we propose to use a recently developed nonparametric procedure: the Local Adaptive Volatility Estimation (LAVE). The motivation for using this method is to avoid a possible model misspecification for the conditional variance. In a second step, we suggest a set of estimation and model selection procedures tests based on the so-obtained residuals. These methods enable to assess the global fit of a given distribution as well as to focus on its behaviour in the tails. Finally, we illustrate our methodology on three time series (UBS stock returns, BOVESPA returns and EUR/USD exchange rates). [less ▲]

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See detailLe coût CO2 du réveillon de Noël : au Carrefour des choix
Ozer, Pierre ULg

Article for general public (2013)

« L’entrepreneuriat durable implique un large choix de produits durables, que vous trouvez dans nos magasins. Nous vous proposons une vaste gamme de produits bio, écologiques et issus du commerce ... [more ▼]

« L’entrepreneuriat durable implique un large choix de produits durables, que vous trouvez dans nos magasins. Nous vous proposons une vaste gamme de produits bio, écologiques et issus du commerce équitable à un prix qui cadre dans votre budget. Nous mettons aussi régulièrement nos produits locaux en exergue. Car acheter local, c’est acheter plus écologique. » « Tous ensemble, nous nous efforçons de limiter au maximum nos émissions de CO2. » Voici donc ce que l’on trouve sur le site internet de Carrefour Belgium. Vérification de cet engagement par l’absurde… [less ▲]

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See detailDrowsiness monitoring for road safety
Wertz, Jérôme ULg; François, Clémentine ULg; Verly, Jacques ULg

Conference (2013, December 13)

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