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See detailReconstruction of a human mitochondrial complex I mutation in the unicellular green alga Chlamydomonas.
Larosa, Véronique ULg; Coosemans, Nadine ULg; Motte, Patrick ULg et al

in Plant Journal (The) (2012), 70

Defects in complex I (NADH:ubiquinone oxidoreductase) are the most frequent cause of human respiratory disorders. The pathogenicity of a given human mitochondrial mutation can be difficult to demonstrate ... [more ▼]

Defects in complex I (NADH:ubiquinone oxidoreductase) are the most frequent cause of human respiratory disorders. The pathogenicity of a given human mitochondrial mutation can be difficult to demonstrate because the mitochondrial genome harbors large numbers of polymorphic base changes that have no pathogenic significance. In addition, mitochondrial mutations are usually found in the heteroplasmic state, which could hide the biochemical effect of the mutation. We propose that the unicellular green alga Chlamydomonas could be used to study such mutations because (1) respiratory-deficient mutants are viable and mitochondrial mutations are found in the homoplasmic state, (2) transformation of the mitochondrial genome is feasible, (3) Chlamydomonas complex I is close to that of humans. To illustrate that, we have introduced a Leu157Pro substitution in the Chlamydomonas ND4 subunit of complex I of two different recipient strains by biolistic transformation, demonstrating that site-directed mutagenesis of the Chlamydomonas mitochondrial genome is possible. This substitution did not lead to any respiratory enzyme defect when it is present in the heteroplasmic state in a patient presenting chronic progressive external ophthalmoplegia. When present in the homoplasmic state in the alga, the mutation does not prevent the assembly of the 950 kDa whole complex I which conserves nearly all the NADH dehydrogenase activity of the peripheral arm. However, the NADH:duroquinone oxidoreductase activity is strongly reduced, suggesting that the substitution could affect ubiquinone fixation to the membrane domain. The in vitro defects are correlated in vivo with a decrease in dark respiration and growth rate. [less ▲]

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See detailLife-cycle assessment of residential buildings in three different European locations, case study
Rossi, Barbara ULg; Marique, Anne-Françoise ULg; Reiter, Sigrid ULg

in Building & Environment (2012), 51

The paper presents the comparative results of the life-cycle assessment (LCA) of one residential building with two constructive systems in Brussels and one steel frame house located in three different ... [more ▼]

The paper presents the comparative results of the life-cycle assessment (LCA) of one residential building with two constructive systems in Brussels and one steel frame house located in three different European towns: Brussels (Belgium), Coimbra (Portugal) and Luleå (Sweden). In a recent study, a modular building was studied in Coimbra and Luleå. It was shown that in terms of CO2 emissions, the Use Stage was the most harmful stage during the building life-cycle for Coimbra climate. Contrarily, in Luleå, it was the Product Stage, despite energy consumption being higher than Coimbra, due to the way electricity and heat are generated. In the present study, two structural systems are first compared for the Belgian house: steel frame and traditional masonry. A different life-cycle scenario is taken into account for the steel frame house for the three different locations, in which the monthly temperatures, energy mix, heating and cooling systems are defined. The LCA is carried out using the basic tool described in the companion paper. It is worth recalling that the results obtained with the basic tool were verified against Pleiades+Comfie and Equer software, enabling to carry out a complete LCA, for Brussels. Our results confirm that for all the three climates, the Use Stage (Operational energy) is the most harmful period during the building life-cycle and that the energy mix of the country strongly influences the equivalent CO2 emissions related to the Use Stage (Operational carbon) and may entirely reverse the conclusions about the life-cycle carbon footprint of the building. [less ▲]

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See detailConseils pour écrire et évaluer un article scientifique
Cornet, Annie ULg

Diverse speeche and writing (2012)

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See detailTransfer of drug dissolution testing by statistical approaches: Case Study
AL-Kamarany, M. A.; EL Karbane, M.; Ridouan, K. et al

in Saudi Pharmaceutical Journal (2012), 20(1), 93101

The analytical transfer is a complete process that consists in transferring an analytical procedure from a sending laboratory to a receiving laboratory. After having experimentally demonstrated that also ... [more ▼]

The analytical transfer is a complete process that consists in transferring an analytical procedure from a sending laboratory to a receiving laboratory. After having experimentally demonstrated that also masters the procedure in order to avoid problems in the future. Method of transfers is now commonplace during the life cycle of analytical method in the pharmaceutical industry. No official guideline exists for a transfer methodology in pharmaceutical analysis and the regulatory word of transfer is more ambiguous than for validation. Therefore, in this study, Gauge repeatability and reproducibility (R&R) studies associated with other multivariate statistics appropriates were successfully applied for the transfer of the dissolution test of diclofenac sodium as a case study from a sending laboratory A (accredited laboratory) to a receiving laboratory B. The HPLC method for the determination of the percent release of diclofenac sodium in solid pharmaceutical forms (one is the discovered product and another generic) was validated using accuracy profile (total error) in the sender laboratory A. The results showed that the receiver laboratory B masters the test dissolution process, using the sameHPLC analytical procedure developed in laboratory A. In conclusion, if the sender used the total error to validate its analytical method, dissolution test can be successfully transferred without mastering the analytical method validation by receiving laboratory B and the pharmaceutical analysis method state should be maintained to ensure the same reliable results in the receiving laboratory. [less ▲]

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See detail(G)astronomie - Comète glacée
Nazé, Yaël ULg

Article for general public (2012)

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See detailMeasuring Downside and Extreme Risk Allocation in Equity Hedge Funds
Lambert, Marie ULg

E-print/Working paper (2012)

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See detailTransferts de Chaleur et de Matière - Exercices
Mengoni, Marlène ULg; Hogge, Michel ULg

Learning material (2012)

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See detailAnalgésie péridurale obstétricale et lombalgie du post-partum: un lien de cause à effet?
CHARLIER, Vanessa ULg; Brichant, Géraldine ULg; DEWANDRE, Pierre-Yves ULg et al

in Revue Médicale de Liège (2012), 67(1), 16-20

backache is a common problem in the general population. the prevalence of backpain is increased during pregnancy and after delivery. early studies have suggested that labor epidural analgesia might be ... [more ▼]

backache is a common problem in the general population. the prevalence of backpain is increased during pregnancy and after delivery. early studies have suggested that labor epidural analgesia might be associated with an increased incidence of backache in the postpartum period. However, these initial studies were retrospective and their design included several methodological deficiencies. All the prospective studies published afterwards (prospective cohort studies and 3 ran- domized controlled trials) yield the same result : there is no relationship between labor epidural analgesia and long-term postpartum backpain. pregnant women must be aware of this in order to make an informed and appropriate choice about labor epidural analgesia, the most effective technique for intra- partum pain relief. [less ▲]

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See detailSerial FEM/XFEM-Based Update of Preoperative Brain Images Using Intraoperative MRI
Vigneron, Lara; Noels, Ludovic ULg; Warfield, Simon et al

in International Journal of Biomedical Imaging (2012), 2012

Current neuronavigation systems cannot adapt to changing intraoperative conditions over time. To overcome this limitation, we present an experimental end-to-end system capable of updating 3D preoperative ... [more ▼]

Current neuronavigation systems cannot adapt to changing intraoperative conditions over time. To overcome this limitation, we present an experimental end-to-end system capable of updating 3D preoperative images in the presence of brain shift and successive resections. The heart of our system is a nonrigid registration technique using a biomechanical model, driven by the deformations of key surfaces tracked in successive intraoperative images. The biomechanical model is deformed using FEM or XFEM, depending on the type of deformation under consideration, namely brain shift or resection. We describe the operation of our system on two patient cases, each comprising ¯ve intraoperative MR images, and demonstrate that our approach significantly improves the alignment of nonrigidly registered images. [less ▲]

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See detailToward a High Order Throughflow - Investigation of the Nonlinear Harmonic Method Coupled With an Immersed Boundary Method for the Modeling of the Circumferential Stresses
Thomas, Jean-Philippe ULg; Léonard, Olivier ULg

in Journal of Turbomachinery (2012), 134(1),

Capturing a level of modeling of the flow inside a multi-stage turbomachine, such as unsteadiness for example, can be done at different degrees of details, either by capturing all deterministic features ... [more ▼]

Capturing a level of modeling of the flow inside a multi-stage turbomachine, such as unsteadiness for example, can be done at different degrees of details, either by capturing all deterministic features of the flow with a pure unsteady method or by settling for an approximated solution at a lower computational cost. The harmonic methods stand in this second category. Amongst them the "Nonlinear Harmonic Method'' from He revealed the most efficient. This method consists of solving the fully nonlinear 3D steady problem and a linearized perturbation system in the frequency domain. As it has been shown by the authors that the circumferential variations exhibit a harmonic behavior, it is proposed here to adapt this method to the throughflow model, where the main nonlinear system would be the common throughflow equations and the auxiliary system would give access to the circumferential stresses. As the numerical local explicit impermeability conditions are unsupported by Fourier series, the adaptation of this technique to the throughflow model passes through a reformulation of the blade effect by a smooth force field as in the "Immersed Boundary Method'' from Peskin. A simple example of an inviscid flow around a cylinder will illustrate the preceding developments, bringing back the mean effect of the circumferential non uniformities into the meridional flow. [less ▲]

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See detailThe CphAII protein from Aquifex aeolicus exhibits a metal-dependent phosphodiesterase activity
Kupper, Michaël; Bauvois, Cédric; Frère, Jean-Marie ULg et al

in Extremophiles : Life Under Extreme Conditions (2012), 16(1)

The CphAII protein from the hyperthermophile Aquifex aeolicus shows the five conserved motifs of the metallo-β-lactamase (MBL) superfamily and presents 28% identity with the Aeromonas hydrophila subclass ... [more ▼]

The CphAII protein from the hyperthermophile Aquifex aeolicus shows the five conserved motifs of the metallo-β-lactamase (MBL) superfamily and presents 28% identity with the Aeromonas hydrophila subclass B2 CphA MBL. The gene encoding CphAII was amplified by PCR from the A. aeolicus genomic DNA and overexpressed in Escherichia coli using a pLex-based expression system. The recombinant CphAII protein was purified by a combination of heating (to denature E. coli proteins) and two steps of immobilized metal affinity chromatography. The purified enzyme preparation did not exhibit a β-lactamase activity but showed a metal-dependent phosphodiesterase activity versus bis-p-nitrophenyl phosphate and thymidine 5'-monophosphate p-nitrophenyl ester, with an optimum at 85°C. The circular dichroism spectrum was in agreement with the percentage of secondary structures characteristic of the MBL αββα fold. [less ▲]

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See detailMyeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing
Ponthier, Jérôme ULg; Desvals, Maud; Franck, Thierry ULg et al

in Journal of Equine Veterinary Science (2012), (32), 32-37

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated with decreased motility. The aim of this study was to determine kinetics of MPO concentration during freezing, its origin, and its impact on frozenethawed semen. Forty ejaculates were used. Semen was frozen using the classical freezing procedure. MPO concentrations were assayed in fresh semen, after centrifuga- tion, and after cooling down to 4 C. Post-thaw MPO assay results and spermogram characteristics were determined. MPO immunocytochemistry was performed on 4 different ejaculates at each step of freezing procedure. MPO concentration increased after cooling down to 4 C and thawing compared with fresh semen. As temperature decreased, MPO was higher or tended to be higher in post-thaw poor quality samples. Nonsperm cells showed various degrees of MPO immunostaining and were observed as epithelial cells with nuclear pyknosis and keratinization. MPO immunostaining increased in medium and decreased in nonsperm cells during freezing. Our study shows that MPO concentration in equine semen increases when temperature decreases. We hypothesize that nonsperm cells present in fresh semen could release MPO. [less ▲]

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See detailA finite volume based data transfer method for remeshing. Application to metal forming problems
Bussetta, Philippe ULg; Ponthot, Jean-Philippe ULg

in Proceedings of the 18th International Symposium on Plasticity & Its Current Applications (2012, January)

The resolution of many nonlinear solid mechanical problems with the Finite Elements Method requires remeshing when very large deformations occur. The remeshing operation is divided into two parts: the ... [more ▼]

The resolution of many nonlinear solid mechanical problems with the Finite Elements Method requires remeshing when very large deformations occur. The remeshing operation is divided into two parts: the generation of the new mesh and the data transfer from the old to the new mesh. In dynamic plasticity problems, two kinds of fields have to be transferred: the first is defined thanks to the nodal values and the second one is defined at the integration points. These two kinds of fields are transferred thanks to an original computation of the Data Transfer Method based on the Finite Volume Elements. This method is compared to another one using Mortar Elements to solve metal forming problems. [less ▲]

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See detailTables de contingence à trois dimensions : aspects théoriques, applications et analogie avec l'analyse de la variance à trois critères de classification
Claustriaux, Jean-Jacques ULg; Palm, Rodolphe ULg; Ferrandis Vallterra, Salvador ULg et al

in Notes de Statistique et d'Informatique (2012), (1), 19

For contingency table, concepts of independence are not so easy to understand in statistical practice. So, theoretical aspects of the log-linear model are described and applied to a three levels example ... [more ▼]

For contingency table, concepts of independence are not so easy to understand in statistical practice. So, theoretical aspects of the log-linear model are described and applied to a three levels example; an analogy with analysis of variance is also established. [less ▲]

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See detailA regional flux-based risk assessment approach for multiple contaminated sites on groundwater bodies
Jamin, Pierre ULg; Dollé, Fabien ULg; Chisala, Brenda et al

in Journal of Contaminant Hydrology (2012), 127(1-4), 65-75

In the context of the Water Framework Directive (EP and CEU, 2000), management plans have to be set up to monitor and to maintain water quality in groundwater bodies in the EU. In heavily industrialized ... [more ▼]

In the context of the Water Framework Directive (EP and CEU, 2000), management plans have to be set up to monitor and to maintain water quality in groundwater bodies in the EU. In heavily industrialized and urbanized areas, the cumulative effect of multiple contaminant sources is likely and has to be evaluated. In order to propose adequate measures, the calculated risk should be based on criteria reflecting the risk of groundwater quality deterioration, in a cumulative manner and at the scale of the entire groundwater body. An integrated GIS- and flux-based risk assessment approach for groundwater bodies is described, with a regional scale indicator for evaluating the quality status of the groundwater body. It is based on the SEQ-ESO currently used in the Walloon Region of Belgium which defines, for different water uses and for a detailed list of groundwater contaminants, a set of threshold values reflecting the levels of water quality and degradation with respect to each contaminant. The methodology is illustrated with first results at a regional scale on a groundwater body-scale application to a contaminated alluvial aquifer which has been classified to be at risk of not reaching a good quality status by 2015. These first results show that contaminants resulting from old industrial activities in that area are likely to contribute significantly to the degradation of groundwater quality. However, further investigations are required on the evaluation of the actual polluting pressures before any definitive conclusion be established. [less ▲]

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See detailEquine coital exanthema and its potential économic implications for the equine industry
Barrandeguy, M.; Thiry, Etienne ULg

in Veterinary Journal (2012), 191

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