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See detailVolatile organic compounds released by barley roots attract wireworms
Barsics, Fanny ULg; Fiers, Marie ULg; Haubruge, Eric ULg et al

Poster (2012, February 10)

Wireworms are the soil dwelling larvae of click beetles and are pests of many crops worldwide. Alternatives to insecticide treatments are needed in order to develop integrated management strategies. Our ... [more ▼]

Wireworms are the soil dwelling larvae of click beetles and are pests of many crops worldwide. Alternatives to insecticide treatments are needed in order to develop integrated management strategies. Our work consists in elucidating the role of barley root-emitted volatile organic compounds on the orientation behaviour of Agriotes sordidus wireworms. Using a dual choice olfactometer we have evaluated the attractiveness of a variety of baits ranging from barley roots themselves to isolated root-emitted volatile organic compounds. Wireworms were significantly attracted towards most of the tested baits. Our results should be taken into account in varietal selection, in crop rotation, or in trapping systems aiming at the reduction of the populations of this pest. [less ▲]

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See detailA new HPLC method to quantify alliin, the major sulfur compound in garlic
DETHIER, Bérénice ULg; Laloux, Morgan ULg; Hanon, Emilien et al

Poster (2012, February 10)

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized ... [more ▼]

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized. This process leads to two stereoisomers. Their HPLC separation was the aim of this work. The method described in the literature (amino column, detection at 210 nm) showed a resolution of 1,1 between the isomers. A second method was developed: the stationary phase was porous graphitic carbon and a water-ACN gradient was used for the elution. It provided a resolution of 3,2, was shorter and underwent the validation process. The method leads to satisfying results. The separation is excellent, and the validation criteria are fulfilled. This may be valuable for future research on garlic. [less ▲]

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See detailDirect imaging, photometry and spectroscopy of gravitationally lensed quasars
Ricci, Davide ULg

Doctoral thesis (2012)

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See detailReplacing explicit water and membrane molecules in molecular dynamics simulation to boost simulation speed
Steinhauer, Sven ULg; Crowet, Jean-Marc ULg; Lins, Laurence ULg et al

Poster (2012, February 10)

Molecular dynamics (MD) is an appropriate method for investigation of biomolecular systems and helps in explaining results from wet lab experiments or in getting further insight into details, which are ... [more ▼]

Molecular dynamics (MD) is an appropriate method for investigation of biomolecular systems and helps in explaining results from wet lab experiments or in getting further insight into details, which are not accessible by experimental methods(Lindahl, 2008). By now, many biologically relevant processes for drug design, toxicological studies and other fields of application, can not be performed by atomistic MD simulations (Lindahl, 2008). In MD, the necessary time effort for carrying out a simulation is considerable and depends mainly on (1) the complexity of the simulated system (2) the simulated time scale (3) the simulation method (4) the efficiency of used hardware and software algorithms. Carried out MD simulations nowadays may still take weeks of calculation on high end computers. In practice, biologically relevant processes, as e.g. protein folding, take usually place above the time scale of milli seconds. They can take up to the order of some thousands of seconds (in case of the folding of membrane proteins). Molecular dynamics computer simulations have reached the scale of micro seconds for simulations of systems where each atom was described and simulated over time.(Lindahl, 2008) Nevertheless, MD has risen to an important promoter methodology for many different fields of application. By replacing bunches of atoms by artificial particles, complexity of the systems can be reduced. This method is called the coarse grain method (CG). Biggin and Bond (2008) found an acceleration of their simulation processes for self assembling membrane / protein systems in water by factor 100. They estimated one to two days of calculation for a simulated time scale of 0.1 to 0.2 micro seconds for their systems. Implicit force fields like "IMPALA", aim to describe water and/or membrane molecules in simulations by a couple of simple and partially precalculable equations. “IMPALA” is a force field initially developed by our laboratory. Using this method, thousands of water and lipid molecules can be replaced, leading to a reduced complexity of the system to be simulated. "IMPALA"(Ducarme et al., 1998) based on the assumption of rigid peptides and aimed to find the insertion characteristics of such in membranes. Elimination of the necessity for simulating the aqueous and lipid phase atom by atom in the software package "Gromacs"(Berendsen et al., 1995) will permit both: a gain of speed, as it was already the case for the introduction of the coarse grain method, and a gain of precision by turning rigid molecules flexible through "Gromacs". Our current work is the integration of the "IMPALA" implicit force field into "Gromacs". Biggin, P.C. & Bond, P.J. Molecular dynamics simulations of membrane proteins. Methods Mol. Biol. 443, 147-60(2008). Berendsen, et al. (1995) Comp. Phys. Comm. 91: 43-56. Ducarme, P., Rahman, M. & Brasseur, R. IMPALA: a simple restraint field to simulate the biological membrane in molecular structure studies. Proteins 30, 357-71(1998). Lindahl, E.R. (2008). Molecular dynamics simulations. Methods Mol. Biol. 443, 3-23. [less ▲]

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See detailDeux méthodes numériques pour la résolution de problèmes de diffraction multiple à haute fréquence
Antoine, X.; Boubendir, Y.; Geuzaine, Christophe ULg

Scientific conference (2012, February 10)

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See detailVariability among morning and evening milk compositions during the lactation
Arnould, Valérie ULg; Gengler, Nicolas ULg; Soyeurt, Hélène ULg

Poster (2012, February 10)

The main objective of this study was to analyze milking-to-milking variability of milk yield and milk composition (such as fat and protein percentages and somatic cell count). Additional objective was to ... [more ▼]

The main objective of this study was to analyze milking-to-milking variability of milk yield and milk composition (such as fat and protein percentages and somatic cell count). Additional objective was to extend this analyze to the milk fat composition. Milk samples (n=195.960) were collected from 29.636 cows in 491 Luxembourg farms and analyzed by MIR spectrometry. The milk contents of saturated, mono-, poly- and unsaturated fatty acids, and short, medium and long chain fatty acids were predicted from the recorded MIR spectral data. As expected, the milk composition and the milk fat composition, are affected by several factors as the milking period and the days in milk. In practice, using separately milk evening and milk morning could be interesting for cheese or butter production. [less ▲]

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See detailSoil Respiration in forest Ecosystems: Combination of a multilayer Approach and an Isotopic Signal Analysis
Goffin, Stéphanie ULg; Longdoz, Bernard; Maier, Martin et al

in Communications in Agricultural and Applied Biological Sciences (2012, February 10)

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See detailCarbon balance of a grazed grassland in Belgium
Jerome, Elisabeth ULg; Beckers, Yves ULg; Bodson, Bernard ULg et al

in Communications in Agricultural and Applied Biological Sciences (2012, February 10)

This work seeks to analyze the carbon balance of a managed permanent grassland grazed by the “Blanc Bleu Belge” breed of cattle. The study site is located at Dorinne. The experimental field was equipped ... [more ▼]

This work seeks to analyze the carbon balance of a managed permanent grassland grazed by the “Blanc Bleu Belge” breed of cattle. The study site is located at Dorinne. The experimental field was equipped with an eddy covariance system that measured CO2 fluxes and with a micrometeorological station. Carbon exported through mowing, imported through compost application or through complementary feeds for cattle was also measured. Carbon lost through CH4 emissions was finally estimated, allowing the establishment of a full C budget. In addition, the vegetation development was followed in order to estimate herbage production and dry matter intake by grazing animals. First year full carbon budget is presented and discussed, in relation with climate conditions and management practices. [less ▲]

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Peer Reviewed
See detailEstimation of genetic parameters for methane indicator traits based on milk fatty acids in dual purpose Belgian blue cattle
Kandel, Purna Bhadra ULg; Soyeurt, Hélène ULg; Gengler, Nicolas ULg

in Communications in Agricultural and Applied Biological Sciences (2012, February 10), 77(1), 21-25

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid ... [more ▼]

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid based CH4 indicators published in the literature were predicted from milk mid-infrared spectra using 597 calibration samples. For the indicator showing the highest link (R2 =0.88) with SF6 CH4 data, the average daily heritability was 0.21, 0.20 and 0.10 for each lactation, respectively. The sire genetic variability was on average 2.82 kg2 of CH4 per lactation. The genetic difference between the sires having cows eructing higher and lower CH4 was 10 kg of CH4 averaged per lactation. In conclusion, CH4 indicators can be predicted by MIR and the genetic variability of these traits seems to exist. [less ▲]

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See detailEsthétique, affectivité et réflexivité. Essai sur la dimension interculturelle de l'anthropologie existentielle de Jean-Paul Sartre
Nke, Fridolin ULg

Doctoral thesis (2012)

Using existential anthropology in understanding contemporary history I try to reveal the value of the Sartrean model of intentional consciousness in understanding the human relationships and the political ... [more ▼]

Using existential anthropology in understanding contemporary history I try to reveal the value of the Sartrean model of intentional consciousness in understanding the human relationships and the political conflicts of our time. I examine the cultural aspects of Sartre’s thought. Sartre’s existential anthropology of the historical real-life refers to a complex philosophical method inspired by Husserl’s phenomenology, Freud’s psychoanalysis and Marx’s dialectic. It aims at understanding individual’s imaginary and behavior. Using that method, not only are we able to reveal the structure of people’s intentions in building and defending their personal and national interests, but also can we manifest the imaginary that constitute what Sartre designates by Dog’s mind, that meant criminal’s and racist’s beliefs. Sartre used his method in understanding writers as Baudelaire, Flaubert or Genet, who was also a criminal. Our intention is to apply psycho-analysis in understanding great figures of human history, such as Hitler, the “Dog” of our time, so that we shall be able to built harmless intercultural relationships. [less ▲]

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See detailBanana Streak Virus activation by tissue culture in natural interspecific banana hybrids
NINTIJE, Pierre

Poster (2012, February 10)

Banana and plantains are important in Burundi as a staple food crop and source of income for farmers. Nevertheless, their productions are threatened by pathogens among which viruses are especially harmful ... [more ▼]

Banana and plantains are important in Burundi as a staple food crop and source of income for farmers. Nevertheless, their productions are threatened by pathogens among which viruses are especially harmful. Elimination procedures of viruses are mainly performed by banana tissue culture techniques (TC) giving good sanitation results for most bananas’ viruses. However, Banana Streak Virus (BSV) a pararetrovirus is activated by TC for banana with the B genome. Therefore, there is a risk of encouraging the emergence of BSV in Burundi by the distribution of TC products to growers. In this work, we studied the activation of BSV by tissue culture in 5 banana cultivars collected in Burundi and micropropagated in vitro. The BSV activation was demonstrated in 4 cultivars using molecular tools. [less ▲]

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Peer Reviewed
See detailRhizobacterial volatile organic compounds modulate biomass production and root architecture in Arabidopsis thaliana (L.) Heynh.
Varin, Sébastien; Saunier de Cazenave-Mendaluk, Magdalena ULg; Ormeno-Lafuente, Elena et al

Poster (2012, February 10)

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See detailUsing fatty acid contents in milk to improve fertility of dairy cows?
Bastin, Catherine ULg; Berry, D.P.; Soyeurt, Hélène ULg et al

Poster (2012, February 10)

Improving dairy cow fertility by means of genetic selection has become increasingly important over the last years in order to overcome the declining cow fertility. This study investigated whether the ... [more ▼]

Improving dairy cow fertility by means of genetic selection has become increasingly important over the last years in order to overcome the declining cow fertility. This study investigated whether the fatty acids profile in milk could be used as an early predictor of genetic merit for fertility. Genetic covariances among 17 fatty acid contents in milk and the number of days from calving to conception were estimated from 29,792 first-parity Holstein cows. Results substantiated the unfavorable relationship among fertility and body fat mobilization in early lactation. Also, about 75% of the genetic variability of fertility was explained by the variability in milk fatty acids profile over the lactation indicating that these traits could be used to supplement genetic evaluations for fertility. [less ▲]

Detailed reference viewed: 42 (14 ULg)
See detailEndocrinologie et vieillissement
Beckers, Albert ULg

Scientific conference (2012, February 10)

Detailed reference viewed: 13 (1 ULg)