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See detailReconstruction of a human mitochondrial complex I mutation in the unicellular green alga Chlamydomonas.
Larosa, Véronique ULg; Coosemans, Nadine ULg; Motte, Patrick ULg et al

in Plant Journal (The) (2012), 70

Defects in complex I (NADH:ubiquinone oxidoreductase) are the most frequent cause of human respiratory disorders. The pathogenicity of a given human mitochondrial mutation can be difficult to demonstrate ... [more ▼]

Defects in complex I (NADH:ubiquinone oxidoreductase) are the most frequent cause of human respiratory disorders. The pathogenicity of a given human mitochondrial mutation can be difficult to demonstrate because the mitochondrial genome harbors large numbers of polymorphic base changes that have no pathogenic significance. In addition, mitochondrial mutations are usually found in the heteroplasmic state, which could hide the biochemical effect of the mutation. We propose that the unicellular green alga Chlamydomonas could be used to study such mutations because (1) respiratory-deficient mutants are viable and mitochondrial mutations are found in the homoplasmic state, (2) transformation of the mitochondrial genome is feasible, (3) Chlamydomonas complex I is close to that of humans. To illustrate that, we have introduced a Leu157Pro substitution in the Chlamydomonas ND4 subunit of complex I of two different recipient strains by biolistic transformation, demonstrating that site-directed mutagenesis of the Chlamydomonas mitochondrial genome is possible. This substitution did not lead to any respiratory enzyme defect when it is present in the heteroplasmic state in a patient presenting chronic progressive external ophthalmoplegia. When present in the homoplasmic state in the alga, the mutation does not prevent the assembly of the 950 kDa whole complex I which conserves nearly all the NADH dehydrogenase activity of the peripheral arm. However, the NADH:duroquinone oxidoreductase activity is strongly reduced, suggesting that the substitution could affect ubiquinone fixation to the membrane domain. The in vitro defects are correlated in vivo with a decrease in dark respiration and growth rate. [less ▲]

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See detailEngineering and characterisation of chimeric CXCR4 and CXCR7 chemokine receptors
Szpakowska, Martyna ULg; Fievez, Virginie; Counson, Manuel et al

Poster (2012, January)

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See detailTitan airglow: FUV and EUV 2009 UVIS spectra of airglow during day, night and eclipse
Ajello, J.M.; Stevens, M.; West, R. et al

Conference (2012, January)

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See detailLes PCB non apparentés aux dioxines (non dioxin-like PCB) dans les denrées alimentaires et les aliments pour animaux: mise en place d'une législation européenne
Eppe, Gauthier ULg

Article for general public (2012)

La famille des PolyChloroBiphényles ou PCB regroupe un ensemble de 209 composés différents (dénommés usuellement congénères) se différenciant par le nombre (1 à 10 atomes) et la position des atomes de ... [more ▼]

La famille des PolyChloroBiphényles ou PCB regroupe un ensemble de 209 composés différents (dénommés usuellement congénères) se différenciant par le nombre (1 à 10 atomes) et la position des atomes de chlore sur la molécule biphényle. Cette famille de composés peut être divisée en deux catégories selon leurs propriétés toxicologiques, et plus particulièrement les effets tératogènes des PCB. Le premier groupe, composé de 12 congénères, se caractérise par des propriétés toxicologiques similaires aux dioxines, et il est par conséquent désigné par le terme ‘PCB de type dioxine’ (dioxin-like PCB, DL-PCB). L’autre groupe de PCB non apparenté aux dioxines (non dioxin-like PCB, NDL-PCB), présente d’autres mécanismes de toxicité pris en compte dans la gestion du risque PCB. Parmi ces 197 NDL-PCB, une sous-catégorie de six congénères relativement abondants dans l’environnement et dans les chaînes alimentaires (PCB 28, 52, 101, 138, 153 et 180), représente ce que nous appelons communément les PCB indicateurs. De par leurs propriétés de persistance, de bioaccumulation et de biomagnification, l’ensemble des PCB (DL-PCB et NDL-PCB) se retrouve fréquemment dans l’alimentation humaine et dans les aliments pour animaux. La fixation de teneurs maximales en NDL-PCB dans divers aliments fait l’objet d’une nouvelle réglementation européenne qui entrera en vigueur dès 2012. Cet article fait le point sur l’impact de ces nouvelles propositions par rapport au niveau de bruit de fond des principales classes d’aliments mais également sur l’évolution des méthodes d’analyse nécessaires pour atteindre les performances requises. [less ▲]

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See detailUnsteady Navier-Stokes simulation of low-Reynolds stall flutter
Yabili, Sacha ULg; Smith, Marilyn; Dimitriadis, Grigorios ULg

in Proceedings of the 50th AIAA Aerospace Sciences Meeting (2012, January)

A solver has been developed within the OpenFoam framework to compute large amplitude motion of two-dimensional rigid configurations. The results obtained with this code were successfully validated on ... [more ▼]

A solver has been developed within the OpenFoam framework to compute large amplitude motion of two-dimensional rigid configurations. The results obtained with this code were successfully validated on rigid airfoils at static and dynamic conditions, as well as correlated with experimental data and numerical solutions from similar unsteady solvers. The results demonstrate that current computational methods are, within the constraints imposed by spatial grids, temporal integration and turbulence modeling, capable of capturing the self-sustained oscillations characterizing stall flutter event with reasonable accuracy, including the mechanisms of energy transfer. [less ▲]

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See detailThe influence of laboratory-induced Meld score differences on liver allocation: more reality than myth.
Schouten, J. N.; Francque, S.; Van Vlierberghe, H. et al

in Clinical Transplantation (2012), 26(1), 62-70

Background: Liver allocation in Eurotransplant (ET) is based on the MELD score. Interlaboratory MELD score differences in INR and creatinine determination have been reported. The clinical implication of ... [more ▼]

Background: Liver allocation in Eurotransplant (ET) is based on the MELD score. Interlaboratory MELD score differences in INR and creatinine determination have been reported. The clinical implication of this observation has not been demonstrated. Methods: MELD scores were calculated in 66 patients with liver cirrhosis using bilirubin, creatinine, and INR analyzed in six liver transplant centers. Based on allocation results of ET, patients transplanted from December 2006 to June 2007 were divided according to MELD score in four groups. For each group, the influence of the match MELD on the probability of receiving a transplant was studied (Cox proportional hazards model). Results: Laboratory-dependent significant differences in MELD score were demonstrated. Cox proportional hazards model showed a significant association between MELD score and the probability of organ allocation. The unadjusted hazard ratio for receiving a liver transplant was significantly different between group 2 and group 4 (group 2: MELD 19–24; group 4: MELD > 30). Conclusion: Laboratory-dependent significant differences in MELD score were observed between the six transplant centers. We demonstrated a significant association between the MELD score and the probability of organ allocation. The observed interlaboratory variation might yield a significant difference in organ allocation in patients with high MELD scores. [less ▲]

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See detailTransferts de Chaleur et de Matière - Exercices
Mengoni, Marlène ULg; Hogge, Michel ULg

Learning material (2012)

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See detailSynthesis of star and H-shape polymers via a combination of cobalt-mediated radical polymerization and nitrone mediated radical coupling reactions
Detrembleur, Christophe ULg; Debuigne, Antoine ULg; Altintas, Ozcan et al

in Polymer Chemistry (2012), 3(1), 135-147

Via consecutive cobalt-mediated radical polymerization (CMRP), nitrone-mediated radical coupling (NMRC) and copper catalyzed azide-alkyne cycloaddition (CuAAC), polymers with mikto-arm star and H-shape ... [more ▼]

Via consecutive cobalt-mediated radical polymerization (CMRP), nitrone-mediated radical coupling (NMRC) and copper catalyzed azide-alkyne cycloaddition (CuAAC), polymers with mikto-arm star and H-shape architecture were synthesized. Poly(vinyl acetate)40-block-poly(acrylonitrile)78-Co(acac)2 polymers were synthesized via CMRC and subsequently coupled using an alkyne functional nitrone. The coupling efficiency of the NMRC process was assessed employing N-tert-butyl a-phenyl nitrone (PBN), which is structurally very similar to the later employed coupling agent. Generally, coupling efficiencies of close to 90% or higher were observed in all cases. Since the coupling reaction yields triblock copolymers bearing an alkoxyamine functionality (and thus also an alkyne group) in the middle of the chain, well defined PEG conjugates could be obtained via CuAAC. Miktoarm star polymers of the structure (PVAc-b-PAN)2-PEG were generated as well as H-shaped material of the structure (PVAc-b-PAN)2-PEG-(PVAc-b-PAN)2 via conjugation with bifunctional PEG. In all cases, very narrow molecular weight material was obtained. Molecular weight analysis of the intermediate and the final products reveals that the hydrodynamic volume of the miktoarm star and the H-shaped materials is not substantially increased during the final conjugation reaction despite the fact that the absolute molecular weight increases by more than a factor of two in the latter case. Success of the conjugation reactions was confirmed via composition analysis via NMR. [less ▲]

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See detailGestion de la diversité: nouveaux enjeux
Cornet, Annie ULg

Conference (2012, January)

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See detailALE Simulations of Metal Forming Processes
Boman, Romain ULg; Ponthot, Jean-Philippe ULg

in Akhtar S. Khan; Farhoud Kabirian; Jian Liu (Eds.) Non-linear Response of Conventional & Advanced Materials, and Multi-scale Modeling (2012, January)

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See detailThe limits of the economic value in measuring the global performance of social innovation
Mertens de Wilmars, Sybille ULg; Marée, Michel ULg

in Nicholls Alex; Murdock Alex (Eds.) Social innovation : Blurring boundaries to reconfigure markets (2012)

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See detailTable ronde : Comment assurer l’évaluation diagnostique des compétences scolaires ?
Fagnant, Annick ULg; Dierendonck, Christophe

Conference (2012, January)

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See detailOn the advantages of economically designed the Hotelling’s T2 control chart with variable sample sizes and sampling intervals
Faraz, Alireza ULg; kazemzadeh, R. B.; Parsian, Ahmad et al

in Quality & Quantity (2012), 46(1), 39-53

Faraz and Parsian (Statistical Paper, 47: 569–593, 2006) have shown that the double warning lines (DWL) scheme detects process shifts more quickly than the other variable ratio sampling schemes such as ... [more ▼]

Faraz and Parsian (Statistical Paper, 47: 569–593, 2006) have shown that the double warning lines (DWL) scheme detects process shifts more quickly than the other variable ratio sampling schemes such as variable sample sizes (VSS), variable sampling intervals (VSI) and variable sample sizes and sampling intervals (VSSVSI). In this paper, the DWLT2control chart for monitoring the process mean vector is economically designed. The cost model proposed by Costa and Rahim (Journal of Applied Statistics, 28: 875–885, 2001) is used here and is minimized through a genetic algorithm (GA) approach. Then the effects of the model parameters on the chart parameters and resulting operating loss is studied and finally a comparison between all possible variable ratio sampling (VRS) schemes are made to choose the best option economically. [less ▲]

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See detailNumerical computations of hypersonic boundary layer roughness induced transition on a at plate
Serino, Gennaro ULg; Pinna, Fabio; Rambaud, Patrick et al

Scientific conference (2012, January)

The work is focused on numerical simulations of roughness induced transition for hypersonic ow on a at plate wall mounted roughness element. Numerical simulations are compared to experimental results in ... [more ▼]

The work is focused on numerical simulations of roughness induced transition for hypersonic ow on a at plate wall mounted roughness element. Numerical simulations are compared to experimental results in order to resemble the physics highlighted in the tests. In particular, stress has been placed on the detection of the vortices in the wake behind the roughness element and on the onset of transition. [less ▲]

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See detailLa nécessité d'un inventaire du patrimoine culturel immatériel
Lempereur, Françoise ULg

in Géants d'ici et d'ailleurs (2012, January), (6-7), 24-30

Destinée essentiellement aux institutions et groupements qui ont en charge la sauvegarde du patrimoine culturel immatériel, cette présentation rapide justifie l'inventorisation de ce patrimoine, en ... [more ▼]

Destinée essentiellement aux institutions et groupements qui ont en charge la sauvegarde du patrimoine culturel immatériel, cette présentation rapide justifie l'inventorisation de ce patrimoine, en définit les responsabilités et propose une méthodologie de collectage et de mise à disposition publique des contenus [less ▲]

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See detailContribution of GC×GC-TOFMS to tobacco analysis
Brokl, Michal ULg; Bishop; Liu et al

Conference (2012, January)

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See detailMultilocus-based phylogeny and species recognition within the cosmopolitan Peltigera neopolydactyla-dolichorhiza complex
Magain, Nicolas ULg; Miadlikowska, Jolanta; Lutzoni, François et al

Poster (2012, January)

The Peltigera neopolydactyla/dolichorhiza complex is broadly distributed, growing in boreal and temperate regions from northern Norway to southern Chile, as well as in tropical mountains. Observed ... [more ▼]

The Peltigera neopolydactyla/dolichorhiza complex is broadly distributed, growing in boreal and temperate regions from northern Norway to southern Chile, as well as in tropical mountains. Observed morphotype and chemotype variation within this complex suggested the presence of multiple undescribed species. We inferred the phylogeny of Peltigera section Polydactylon with a special focus on the Peltigera neopolydactyla/dolichorhiza complex to determine the full breadth of this species complex, and to assess if taxa from different parts of the worlds but with similar morphological features share a most recent common ancestor. About 525 ITS sequences representing 104 distinct haplotypes were generated for representatives of Peltigera section Polydactylon. We selected a representative of each broadly defined phylotype for which three protein-coding loci: RPB1.1, b-tubulin and EFT2.1 were sequenced. Each of the three protein coding loci provided equivalent or more resolution and support than the ITS locus. The greatest proportion of significantly supported nodes across the tree resulted from β-tubulin alone. Many specimens identified as P. neopolydactyla and P. dolichorhiza are placed outside of this species complex. As currently defined both species represent polyphyletic assemblages of taxa including several potentially undescribed species. Our phylogenies suggest the presence of putatively new species within several complexes across the section. [less ▲]

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See detailAnthracology: past disturbances and vegetation evolution
Morin-Rivat, Julie ULg

in CoForChange - Newsletter (2012)

One of CoForChange aims is to highlight the relationship between human settlement and plant distribution. Recent studies show that anthropogenic disturbances have had an impact on vegetation, including ... [more ▼]

One of CoForChange aims is to highlight the relationship between human settlement and plant distribution. Recent studies show that anthropogenic disturbances have had an impact on vegetation, including enabling the expansion of light-demanding species (see Newsl. No 4). To contribute to this analysis in a context of deep scarcity of anthracological data in Africa, we have been studying the charcoals from nine soil pits of the CoForChange project as part of a Master’s. The objective was to understand past and present species composition of the forests in relation with past disturbances. Two anthracological protocols of identification were tested: 1) at species level, by describing the anatomical characteristics of charcoals, by carrying investigations with InsideWood online database, and by comparing anatomical data at XylariumTervuren; 2) at community level, by searching for statistical relationships between ecological traits of species and wood anatomy. The results show that the taxonomic diversity is greater in Marantaceae forests than in lowland forests. Three identifications were obtained from 48 described taxa: Gilbertiodendron dewevrei under G. dewevrei monodominant forest (GIB1 at 40 cm depth, 1510 ± 30 years cal. BP), Millettia drastica and Pterocarpus soyauxii inMarantaceae open forests (F9 at 40 cm, 1200 ± 30 cal. BP). Our hypothesis is that monodominant stands of G. dewevrei are relatively stable. With regard to P. soyauxii, the proportion of this lightdemanding species seems to be decreasing over time (at levels from 20 to 40 cm) for the benefit of Marantaceae. Another result highlights the absence of Triplochiton scleroxylon under T. scleroxylon, which may confirm the hypothesis of the recent settlement of this species. Results on the relationship between wood anatomy and functional traits are still preliminary, and further research will be conducted through a PhD programme. The on-going developments of anthracology in tropical Africa will help to identify a larger number of collected samples, and thus to understand better the evolution of tropical forests. [less ▲]

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