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See detailIngénierie génétique d'une β2-intégrine LFA-1 bovine résistante à la leucotoxine de Mannheimia haemolytica
Fett, Thomas ULg

Doctoral thesis (2012)

Domesticated bovines are known since decades to be prone to bacterial pneumonias. Among the causative agents, a consensus emerged stating that Mannheimia haemolytica is the most frequent bacterium ... [more ▼]

Domesticated bovines are known since decades to be prone to bacterial pneumonias. Among the causative agents, a consensus emerged stating that Mannheimia haemolytica is the most frequent bacterium isolated from bovine lungs throughout the world. Moreover, it appeared that its virulence specifically targets ruminant lungs in vivo and ruminant leucocytes in vitro. When the thesis was started, the two main actors underlying this species-specific virulence were known: the leukotoxin (LKT) on the pathogen side and the beta2-integrin LFA-1 on the host side. The objective of the thesis was to contribute to the understanding of the LKT/LFA 1 interaction at the molecular level. Using a between-species perspective, we showed that (i) the CD11a subunit of the LFA-1 heterodimer was not involved in the LKT-specificity for ruminant LFA-1, (ii) the EGF-3 module within the CD18 controls the susceptibility of any given CD18 to LKT and (iii) a non cleavable signal peptide conjugated to a LKT susceptible EGF-3-containing CD18 exacerbates LKT pronecrotic effects. [less ▲]

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See detailP.ParisBnF inv. Suppl. gr. 1385. Un nouveau papyrus homérique
Carlig, Nathan ULg

Scientific conference (2012, February 17)

Description, édition, identification et commentaire du P.ParisBnF inv. Suppl. gr. 1385 (inédit), conservé à la Bibliothèque nationale de France et contenant un passage de l'Iliade d'Homère, réunion avec ... [more ▼]

Description, édition, identification et commentaire du P.ParisBnF inv. Suppl. gr. 1385 (inédit), conservé à la Bibliothèque nationale de France et contenant un passage de l'Iliade d'Homère, réunion avec un autre fragment de papyrus provenant de la même entité bibliologique et présentation des outils de la papyrologie littéraire, notamment informatiques (Base de données Mertens-Pack³ et Leuven Database of Ancient Books). [less ▲]

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See detailCONTROVERSES SUR LA CONFIGURATION DES TERRITOIRES INDIGÈNES ET DES AIRES PROTÉGÉES
Escobar Jimenez, Kelly ULg

Learning material (2012)

Le document explique quel est le rôle des objets intermédiaires dans la construction de frontières en Amazonie équatorienne. Les dispositifs et instruments mobilisés dans la gestion de ces territoires ... [more ▼]

Le document explique quel est le rôle des objets intermédiaires dans la construction de frontières en Amazonie équatorienne. Les dispositifs et instruments mobilisés dans la gestion de ces territoires stabilisent ou rendent controversé la configuration des limites territoriales. [less ▲]

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See detailContribution à l'étude des propriétés tensioactives et membranaires d'esters dérivés de la glycine bétaïne
Nsimba Zakanda, Francis ULg

Doctoral thesis (2012)

The objective of this doctoral research is to contribute to the study of surface active and membrane properties of esters derived from glycine betaine. In this context, esters of glycine betaine ... [more ▼]

The objective of this doctoral research is to contribute to the study of surface active and membrane properties of esters derived from glycine betaine. In this context, esters of glycine betaine (alkylbétaïnate chlorides: CnBC, n = 10-16) were obtained using a new synthetic methodology. Glycine betaine (activated by thionyl chloride) and the primary fatty alcohols were used as basic raw materials for the chemical synthesis of CnBC. Some parameters to optimize the synthesis were studied, such as the nature of the organic solvent, the temperature, the molar ratio of reactants and the concentration of the latter in the reaction medium. Next, the surface active and membrane properties of CnBC were characterized using several instrumental techniques such as Langmuir film balance, isothermal titration calorimetry, fluorescence spectrometry and zeta potential and particle size measurements. Hexadécylbétaïnate chloride (C16BC) was synthesized with better performance under the following conditions: absence of a catalyst/base, a solvent of medium polarity (2-methyl-2-butanol), a temperature of 45°C, a molar ratio of betainyl chloride/1-hexadecanol of 3/1 and a concentration of reactants of 0.132 mol/L. Decylbetainate chloride (C10BC) dodecylbetainate chloride (C12BC) and tetradecylbetainate chloride (C14BC) were obtained using the optimal conditions for the synthesis of C16BC. Concerning the interfacial organization of CnBC, C10BC and C12BC did not form insoluble monolayers at the air-water at 20°C compared to their counterparts, C14BC and C16BC. The study of the monolayer of C16BC in various conditions of the aqueous subphase showed that the pH and monovalent anions (OHˉ, Brˉ, NO3ˉ, ClO4ˉ) did not influence the behavior of the monolayer compared to that in water with Clˉ as counterion while the temperature beyond 25°C, the divalent anions (HPO42-, SO42-) and the ionic strength of sodium sulfate (Na2SO4) affected it in term of interfacial area occupation of the molecules. Interactions between CnBC and model membranes have shown that in water, CnBC, regardless of the length of the alkyl chain, have highly penetreted into the negatively charged lipid monolayers (dipalmitoylphosphatidylserine and dipalmitoylphosphatidic acid), whereas their penetration was average and low into monolayers formed by lipids with small polar head (dipalmitoylphosphatidylethanolamine and cholesterol, CHOL) and lipid with large polar head (palmitoyloleoylphosphatidylcholine, POPC), respectively. In salted buffer, the adsorption of C16BC at the air-water interface and onto the surfaces of the lipid monolayers was instantaneous, and in all cases, the penetrating power was above the presumed pressure of biological membranes. C16BC formed miscible and stable mixtures with POPC and sphingomyelin while an almost ideal behavior was observed for its mixtures with CHOL. An affinity of C16BC for the membrane bilayer containing CHOL was also observed. The adsorption of CnBC onto the surfaces of bilayers was alkyl chain-dependent. C10BC, C14BC and C16BC did not significantly perturbed membranes even at high surfactant concnetrations compared to C12BC that has highly perturbed membranes without solubilizing them. In all cases, particles of positive charge and larger than those of membrane bilayers prepared were formed in the presence of high amounts of CnBC. [less ▲]

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See detailChimie et Science des Matériaux à l'Université de Liège
Léonard, Grégoire ULg

Conference given outside the academic context (2012)

Présentation du métier d'ingénieur en chimie et science des matériaux à des étudiants de secondaire.

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See detailQuand un revenu est-il imposable?
Bourgeois, Marc ULg; Herve, Luc ULg

Scientific conference (2012, February 16)

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See detailIMONIC Spin-off Project
Goffin, Dorothée ULg

Speech/Talk (2012)

Présentation du projet de spin-off IMONIC à un jury de sélection

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See detailAuto-immunité en thyroïdologie et en gastro-entérologie
VALDES SOCIN, Hernan Gonzalo ULg; Beckers, Albert ULg

Conference (2012, February 16)

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See detailDYSPHAGIE, DEGLUTITION ET ALIMENTATION
ALLEPAERTS, Sophie ULg; JAMAR, Marie-Cécile ULg; DELCOURT, Sandrine ULg et al

Conference (2012, February 16)

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Peer Reviewed
See detailVolumetric method of moments and conceptual multilevel building blocks for nanotopologies
Zheng, X.; Valev, V.K.; Verellen, N. et al

in IEEE Photonics Journal (2012), 4(1), 267-282

Based on the relationship between charge dimensionality and singular field behavior, it is proven that in a volumetric description of a volume current carrying topology, half rooftops of different binary ... [more ▼]

Based on the relationship between charge dimensionality and singular field behavior, it is proven that in a volumetric description of a volume current carrying topology, half rooftops of different binary hierarchical level are allowed without introducing numerical difficulties. This opens the possibility to use a very efficient multi-level hierarchical meshing scheme in a Volumetric Method of Moments (MoM) algorithm. The new meshing scheme is validated by numerical calculations and experiments. It paves the way towards a much more efficient use of MoM in the description of arbitrarily shaped nano-structures at IR and optical frequencies. [less ▲]

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Peer Reviewed
See detailA cognitive psychopathological approach to hallucinations
Laroi, Frank ULg

Conference (2012, February 16)

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See detailDebt and Pension
Artige, Lionel ULg; Cavenaile, Laurent; Pestieau, Pierre ULg

Conference (2012, February 15)

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See detailHuntington’s Disease: From the Physiological Function of Huntingtin to the Disease
Borgs, Laurence ULg; Godin, Juliette ULg; Malgrange, Brigitte ULg et al

in Huntington's Disease - Core Concepts and Current Advances (2012)

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Peer Reviewed
See detailFirst-principles study of structural and vibrational properties of SrZrO3
Amisi, Safari ULg; Bousquet, Eric ULg; Katcho, Karume et al

in Physical Review. B, Condensed Matter and Materials Physics (2012), 85

Using first-principles calculations, we investigate the electronic, structural, and vibrational properties of SrZrO3. We start from the high-symmetry cubic perovskite phase, for which the phonon ... [more ▼]

Using first-principles calculations, we investigate the electronic, structural, and vibrational properties of SrZrO3. We start from the high-symmetry cubic perovskite phase, for which the phonon dispersion curves are reported.We point out the coexistence of structural antiferrodistortive instabilities at the R and M zone-boundary points and a ferroelectric instability at the zone center.We showthat the strong antiferrodistortive motions suppress ferroelectricity and are responsible for the orthorhombic ground state as in CaTiO3. The structural properties <br />of possible intermediate phases and of the orthorhombic Pnma ground state are reported. For the latter, an assignment of IR and Raman zone-center phonon modes is proposed. The main features of the ferroelectric instability are also discussed, and we show that a ferroelectric ground state can even be induced in SrZrO3 by strain engineering. [less ▲]

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See detailContribution à l'étude de la surveillance de la mécanique ventilatoire du nouveau-né ventilé.
RIGO, Vincent ULg

Doctoral thesis (2012)

Mechanical ventilation, still a major intervention to improve prognosis in newborns, requires careful monitoring of ventilated infants. This monitoring integrates different parameters. Its classical focus ... [more ▼]

Mechanical ventilation, still a major intervention to improve prognosis in newborns, requires careful monitoring of ventilated infants. This monitoring integrates different parameters. Its classical focus is on blood gases and their proxy (pulse oxymetry, transcutaneous oxygen and carbon dioxide content), and also includes physical assessment, thoracic imaging and appraisal of ventilator settings. Use of currently available on-line respiratory mechanics (RM) as displayed by ventilators seems limited given a large apparent variability. As current respiratory support strategies aim to reduce exposure to mechanical ventilation and to decrease ventilator associated lung injuries, additional continuous monitoring tools could benefit neonatal patients. In a review of advanced biomedical devices in use in the neonatal intensive care units and areas where improvement or evaluation is necessary, the National Institute for Child Health and Human Development underlines simple tools for continuous assessment of vital pulmonary functions at the bedside. This research aims at finding solutions to that problem. In a first step, different respiratory mechanics parameters (dynamic compliance –Cdyn, dynamic resistance –Rdyn, tidal volume –VT and the overdistension parameter C20/C) are obtained from ventilatory recordings of newborns under respiratory support with the most commonly used neonatal ventilator to evaluate their clinical relevance. Those data present a high variability and therefore lack precision. It is possible to mathematically decrease this variability by using parameters averaged over a few minutes and to obtain reproducible results. Continuous pressure, flow and volume data from the ventilator allow construction of pressure-volume, pressure-flow and flow-volume loops. From those loops, Cdyn, Rdyn and VT can be computed by the Mead-Whittenberger method. Those values when derived from respiratory cycles with good appearance significantly differ from ventilator values. Given the lack of precision of ventilator derived respiratory mechanics data, a new strategy is developed to obtain those parameters only from optimal looking respiratory cycles. A new software is designed to reconstruct waves and loops from the ventilator continuous recordings. This software individualises respiratory cycles and compute Cdyn and Rdyn (least mean square method), VT and C20/C. Using 10 sets of two recordings (one in Synchronized Intermittent Mandatory Ventilation and one in Assist/Control ventilatory modes), visual evaluation of 11274 respiratory cycles selects 4847 cycles considered optimal looking. Those assisted cycles present no or minimal leak, good hysteresis of the pressure-volume loop, and no abnormalities of the flow curves. The coefficients of variation of the respiratory mechanics parameters obtained with this method are significantly decreased, by 25-27% from the ventilator values for Rdyn, Cdyn and C20/C, and by 60% for VT. This increase in parameters precision is associated with an improved capacity to discriminate different values. Analysis of discordant values between ventilator and optimal respiratory cycles is relevant. In A/C mode, the VTs from the selected respiratory cycles are lower than values reported by the ventilator, suggesting that currently available VTs give incomplete information for adjustment of ventilator settings. In SIMV mode, the weak correlation between Cdyn from both methods leads to question the relevance of ventilator informations. The important scattering of ventilator C20/Cs out of classical values, and the absence of correlation with values from selected respiratory cycles demonstrate the lack of validity of ventilator C20/Cs. Overall, the results suggest that the use of data derived from selected respiratory cycles could underlie the conception of RM monitoring tools to support ventilatory management. To avoid the heavy workload associated with visual respiratory cycles’ selection, the software is improved to automatically identify optimal cycles. The positive predictive values and specificity of this selection are high. Respiratory mechanics parameters from cycles selected automatically are very concordant with those from visually selected cycles. The last step of this work assesses the software potential with analysis of 21 recordings from various clinical situations. The discriminating power of automatically selected respiratory cycles’ parameters is tested in an extended population. Trending abilities of those parameters are evaluated. Analyses of respiratory mechanics parameters derived from automatically selected cycles are able to demonstrate differences of 4.6-7.1% and more between parameters from two 10min recordings. Averaging data over 3-7min allows to determine a 10% difference. Parameters averaged over 10min allow detection of 10% changes in most patients. Those results should allow building trend curves with clinically and statistically significant informations. In conclusion, the continuous respiratory mechanics analysis software developed and evaluated in this work should give precise informations on the dynamic evolution of RM parameters. Functions integrated in the last version of the software give immediate research opportunities, and should lead to clinical application in a very near future. Those parameters could then complete current informations integrated in ventilatory management. [less ▲]

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See detailRessources dans le rouge pour les technologies vertes ?
Pirard, Eric ULg

Conference given outside the academic context (2012)

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See detailImpact of glycerol and storage temperature on gluatathione concentration and physiological state of Pseudomonas fluorescens BTP1 freeze-dried
Mputu Kanyinda, Jean-Noël ULg; Pierart, C.; Delvigne, Frank ULg et al

Poster (2012, February 15)

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze ... [more ▼]

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze-drying is a commonly used method to preserve bacteria. However, freeze-drying damages the cells, which results in loss of viability. Protective compounds are used to reduce loss of viability during process (freeze-drying and storage). In our study we used flow cytometry analysis to assess the physiological state in which cells are at the end of freeze-drying and Glutathione (GSH) was measured before and during storage. [less ▲]

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