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See detailDegradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULiege; Lallemand, Jérôme ULiege; Périlleux, Claire ULiege et al

Poster (2012, April 18)

Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems ... [more ▼]

Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems. The plant-based production of mAbs, which are multimeric glycoproteins, require their targeting to the secretory pathaway where they are properly folded and matured. However, co-secretion of endogenous proteases, which can represent up to 10% of the extracellular proteins (secretome), is known to significantly alter the yield and quality of secreted mAbs. In this study, we analyzed the proteolytic activities in root-secretome of Arabidopsis thaliana and Nicotiana tabacum. Root-secretomes were recovered by salt extraction and the protease activity was assayed in vitro or by zymography, in a range of pH. The relative contribution of protease classes was evaluated with specific inhibitors. [less ▲]

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See detailSynthesis and evaluation of boronic acids as inhibitors of Penicillin Binding Proteins of classes A, B and C
Zervosen, Astrid ULiege; Sauvage, Eric ULiege; Bouillez, André ULiege et al

Poster (2012, April 18)

The widespread use of beta-lactam antibiotics has lead to the worldwide appearance of drug-resistant strains. Bacteria have developed resistance to beta-lactams by three main mechanisms: the production of ... [more ▼]

The widespread use of beta-lactam antibiotics has lead to the worldwide appearance of drug-resistant strains. Bacteria have developed resistance to beta-lactams by three main mechanisms: the production of beta-lactamases that catalyze hydrolysis of beta-lactams, the production of low-affinity, drug resistant Penicillin Binding Proteins (PBPs) and the over expression of resistant PBPs. PBPs are interesting targets because they catalyse the last steps of the biosynthesis of peptidoglycan, which is unique in bacteria and has no mammalian analogs, outside the cytoplasmic membrane. Various non-ß-lactam inhibitors of PBPs have been developed with the objective of attempting to stall the development of ß-lactam resistance. Boronic acids are potent beta-lactamase inhibitors and have been shown to display some specificity for soluble transpeptidases and PBPs, but their potential as inhibitors of the latter enzymes is yet to be widely explored. Recently, a (2, 6-dimethoxybenzamido)methylboronic acid was identified as being a potent inhibitor of Actinomadura sp. R39 transpeptidase (IC50: 1.3 µM). Here, we will discuss the synthesis of a number of acylaminomethylboronic acids, analogs of (2, 6-dimethoxybenzamido)methylboronic acid, and their potential as inhibitors of PBPs. Several boronic acids of this library were able to inhibit PBPs of classes A, B and C from penicillin sensitive strains. Thus (2-nitrobenzamido)methylboronic acid was identified as a good inhibitor of class A PBP (PBP1b from S. pneumoniae, IC50 = 26 µM), class B PBP (PBP2xR6 from S. pneumoniae, IC50 = 138 µM) and class C PBP (R39 from Actinomadura sp., IC50 = 0.6 µM). Crystal structures of complexes of R39 and PBP1b with boronic acid analogs of our library have already been solved and allowed an interpretation of results. We believe that this work opens new avenues towards the development of molecules that will inhibit PBPs, and eventually display bactericidal effect, on distinct bacterial species. [less ▲]

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See detailReading an Arthurian Prose Romance through its Textual Tradition.
Morato, Nicola ULiege

Scientific conference (2012, April 18)

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See detailHemocompatibility of nanocarriers designed to transport biopharmaceutical drugs
Sevrin, Chantal ULiege; Cerda, Bernardino; Lombart, François ULiege et al

Poster (2012, April 18)

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed ... [more ▼]

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed the large surface they are able to expose to the biological environment promotes their interaction with various biochemicals, in particular proteins which can after adsorption elicit the activation of biological cascades either responsible from NP clearance or/and harmful body reaction (inflammatory / coagulation). In the frame of the European Integrated Project : “Nanobiopharmaceutics”, we have the opportunity to compare the hemoreactivity of about 145 different NP samples differing in core and surface chemistry and classified according to their expected difference in hydrophobicity based on the nature of their core materials. According to this classification, PLGA nanoparticles, polyglycidol-polyethyethylene oxide nanoparticles, polyglycidol thyolated or polyacrylamide nanogels, and polyelectrolyte complexes either based on polyamidoamine or poly(N,N-dimethylamino-2-ethylmethacrylate) have been evaluated within a concentration ranging from 0.3 to 1000 =g/mL. These in vitro tests have been realized for screening purpose adopting normal human bloods and according to Iso 10993. As a summary of this extensive study, our results clearly highlight that most of the polymeric nanoparticles evaluated give rise to some alterations of the blood components. In particular the platelets, intrinsic pathway of coagulation and complement activation are the most reactive biological parameters in the presence of these nanostuctures. Although not strictly related to the surface chemistry our classification has also allowed us to derive some clear correlations between nanomaterial properties and their hemoreactivity. Within the class of polyelectrolyte electrolyte complexes, the modifications brought in the surface chemistry has drastically improved their hemoreactivity. [less ▲]

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See detailNew alginate-chitosan hydrogel to repair cartilage
Henrotin, Yves ULiege

Conference (2012, April 18)

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See detailL'enfermement des mineurs: pour ou contre?
Mormont, Christian ULiege

Conference given outside the academic context (2012)

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See detailClostridium difficile in farm and slaughter animals in Belgium: detection and characterization
Rodriguez Diaz, Cristina ULiege; Taminiau, Bernard ULiege; Van Broeck, Johan et al

Poster (2012, April 18)

Detailed reference viewed: 17 (2 ULiège)
See detailPsychrotrophic and psychrophylic Clostridium responsible for meat spoilage
Rodrigues, A.; Taminiau, Bernard ULiege; Daube, Georges ULiege

Poster (2012, April 18)

Detailed reference viewed: 9 (0 ULiège)
See detailEtudes des systèmes de défenses antitoxiques chez l’amphipode Gammarus roeseli : Effets du parasitisme et d’une exposition au cadmium.
Gismondi, Eric ULiege

Doctoral thesis (2012)

Pour faire face à des perturbations environnementales, les organismes ont développé des défenses antitoxiques couramment utilisés comme biomarqueurs dans l’évaluation de la qualité des milieux. Cependant ... [more ▼]

Pour faire face à des perturbations environnementales, les organismes ont développé des défenses antitoxiques couramment utilisés comme biomarqueurs dans l’évaluation de la qualité des milieux. Cependant, de nombreux facteurs confondants comme la température ou le genre, influencent la réponse de ces biomarqueurs. Il est ainsi nécessaire de connaitre les effets de ces facteurs afin d’imputer correctement la réponse biologique mesurée à la présence de polluants. Dans ce contexte, nous avons choisi d’étudier l’influence d’un parasite acanthocéphale, Polymorphus minutus, transmis horizontalement et de microsporidies à transmission verticale, sur les réponses physiologiques de leur hôte, Gammarus roeseli, un crustacé amphipode d’eau douce d’intérêt écotoxicologique. Pour cela, nous avons choisi d’étudier le glutathion, tripeptide jouant un rôle central dans les systèmes antitoxiques, sa synthèse (i.e. activité γ-glutamylcystéine ligase), les réserves énergétiques (i.e. lipides, glycogène, protéines) et un biomarqueur d’effets toxiques, le malondialdéhyde. L’influence du parasitisme a été appréhendé dans différents cas d’études: (i) chez G. roeseli infecté par P. minutus, (ii) chez G. roeseli infecté par des microsporidies (Dictyocoela roeselum essentiellement) et (iii) chez G. roeseli co-infecté par ces deux types de parasite. Chaque cas d’étude a été réalisé en absence de stress et lors d’une exposition au cadmium. Nous avons mis en évidence qu’en absence de contamination, la présence de P. minutus et une co-infection par P. minutus et D. roeselum affectent les biomarqueurs de G. roeseli. Après exposition au cadmium, la présence de parasites (i.e. infection simple ou co-infection) modifie la mobilisation des défenses antitoxiques et accentue les effets toxiques subits par l’hôte. Les résultats obtenus au cours de ce travail mettent en avant le caractère confondant du parasitisme en écotoxicologie et souligne l’importance de prendre en compte ce paramètre lors de l’évaluation de la qualité des milieux. [less ▲]

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See detailAnémies et insuffisance rénale : quoi de neuf?
Krzesinski, Jean-Marie ULiege

Conference (2012, April 17)

Detailed reference viewed: 19 (0 ULiège)
See detailNon-conventional Political Participation as an outcome of Discrimination Feeling
Gavray, Claire ULiege; Born, Michel ULiege

Scientific conference (2012, April 17)

Detailed reference viewed: 39 (3 ULiège)
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See detailHistoire d'un ElDorado - Cinq siècles d'exploration du sous-sol sud-américain
Pirard, Eric ULiege

Scientific conference (2012, April 17)

Une présentation du rapport de l'Amérique Latine au richesses de son sous-sol, depuis la découverte de l'Amérique jusqu'à aujourd'hui.

Detailed reference viewed: 59 (12 ULiège)
See detailRaman spectroscopy, a tool for biochemical applications
Malherbe, Cédric ULiege

Scientific conference (2012, April 17)

Detailed reference viewed: 16 (8 ULiège)
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See detailConstruire son langage, une nouvelle conception de l’acquisition langagière: données développementales et approche en pathologie langagière
Leroy, Sandrine ULiege; Maillart, Christelle ULiege

Scientific conference (2012, April 17)

Cette conférence traite des répercussions sur notre pratique logopédique d’une nouvelle conception du développement langagier. Celle-ci s’intéresse à la construction du langage oral chez l’enfant. Elle ... [more ▼]

Cette conférence traite des répercussions sur notre pratique logopédique d’une nouvelle conception du développement langagier. Celle-ci s’intéresse à la construction du langage oral chez l’enfant. Elle considère que l’enfant est l’acteur de son développement langagier ; il construit progressivement son langage à partir de ses propres productions antérieures. Cette nouvelle conception issue de la linguistique cognitivo-fonctionnelle s’oppose donc à la conception innéiste (ou générativiste) qui considère que l’enfant dispose de connaissances langagières innées. Cette approche constructiviste nous offre des perspectives théoriques et thérapeutiques très intéressantes pour étudier la pathologie langagière. Des données récentes récoltées auprès d’enfants tout-venant sont présentées. Par la suite, nous abordons plus spécifiquement la dysphasie. Nous tentons de comprendre comment ces théories permettent d'expliquer les difficultés langagières des enfants dysphasiques. Enfin, nous envisageons quels sont les éléments dont il faut tenir compte dans notre pratique logopédique, à la lumière de cette nouvelle conception développementale. [less ▲]

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See detail"Enjeux et défis de l'enseignement des langues dans le contexte de la mondialisation"
Defays, Jean-Marc ULiege; Maréchal, Marielle ULiege; Louis, Vincent

Scientific conference (2012, April 17)

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See detailDu diagnostic à l'intervention en entreprise: quelle réalité, quelles limites?
Hansez, Isabelle ULiege

Conference (2012, April 17)

Detailed reference viewed: 38 (5 ULiège)
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See detailCarrier and aberrations removal in interferometric fringe projection profilometry
Blain, Pascal ULiege; Michel, Fabrice; Renotte, Yvon ULiege et al

in Gorecki, Christophe; Asundi, Anand K.; Osten, Wolfgang (Eds.) Optical Micro- and Nanometrology IV (SPIE proceedings vol.8430) (2012, April 17)

A profilometer which takes advantage of polarization states splitting technique and monochromatic light projection method as a way to overcome ambient lighting for in-situ measurement is under development ... [more ▼]

A profilometer which takes advantage of polarization states splitting technique and monochromatic light projection method as a way to overcome ambient lighting for in-situ measurement is under development [1, 2]. Because of the Savart plate which refracts two out of axis beams, the device suffers from aberrations (mostly coma and astigmatism). These aberrations affect the quality of the sinusoidal fringe pattern. In fringe projection profilometry, the unwrapped phase distribution map contains the sum of the object’s shape-related phase and carrier-fringe-related phase. In order to extract the 3D shape of the object, the carrier phase has to be removed [3, 4]. An easy way to remove both the fringe carrier and the aberrations of the optical system is to measure the phases of the test object and to measure the phase of a reference plane with the same set up and to subtract both phase maps. This time consuming technique is suitable for laboratory but not for industry. We propose a method to numerically remove both the fringe carrier and the aberrations. A first reference phase of a calibration plane is evaluated knowing the position of the different elements in the set up and the orientation of the fringes. Then a fitting of the phase map by Zernike polynomials is computed [5]. As the triangulation parameters are known during the calibration, the computation of Zernike coefficients has only to be made once. The wavefront error can be adjusted by a scale factor which depends on the position of the test object. [less ▲]

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See detailTHE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION
Shafiei, Rasoul ULiege; Thonart, Philippe ULiege

in THE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION (2012, April 17)

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular ... [more ▼]

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular functions of acetic acid bacteria. In previous studies in CWBI, Acetobacter senegalensis was used for production of dried vinegar starters; however the impact of stressors (ethanol and acetic acid) on A. senegalensis remained unclear. In this study, different techniques such as flow cytometry, culturability on solid medium and 2-DiGE were used comparatively to investigate the effect of carbon sources of inoculum media on the tolerance of A. senegalensis to stressors. Analysis of respiration system by flow cytometric methods showed that the presence of 2% (v/v) acetic acid in inoculum medium, in one hand, causes 80% of cells to continue to do respiration after a sudden exposure to 1- 3% (v/v) acetic acid in stress media while 89.7% of cells grown in glucose appeared as dead cells after an abrupt exposure to 3%(v/v) of acetic acid. On the other hand, 59.2% and 49.33% of cells grown in the presence of 2% (v/v) of acetic acid could maintain their entire membrane integrity after exposure to 1% and 3% (v/v) of acetic acid, respectively. Inoculum medium contained 5% (v/v) of ethanol as a carbon source enabled about 90% of cells to keep their growing capacities after a sudden exposure to 3% acetic acid. In contrast, just 40% of cells grown in glucose as a carbon source maintained their culturability on solid medium after exposure to 1% acetic acid. A similar profile of culturability was observed for the cells grown in 5% (v/v) ethanol or 2% (v/v) of acetic acid. A proteomic approach (2-DiGE) was used to analyze proteins expressed in the presence of different carbon sources. Differentially expressed proteins were mainly associated with energy metabolism, carbohydrate metabolisms, folding, sorting and degradation processes. The relative abundance of proteins was extensively different for cell grown in glucose compared with protein contents of cells grown in ethanol or acetic acid. In conclusion, production of a cost effective vinegar starter needs a qualified biomass which tolerates ethanol and acetic acid. Tolerance of A. senegalensis to acetic acid depends to a great extent on the composition of the medium which cells grow in. In spite of low adaption to acetic acid for cell grown in glucose, using ethanol or acetic acid in inoculum media renders a physiological state in A. senegelensis which enables it to cope with higher concentration of acetic acid readily, this biomass has a potential to be used as a starter. [less ▲]

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