References of "2012"
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See detailMethod for producing antibody using "naked" expression vector expressing type II transmembrane fusion protein
Renauld, Jean Christophe; Lemaire, Muriel; Dumoutier, Laurie et al

Patent (2012)

Detailed reference viewed: 45 (4 ULiège)
See detailLeonardo da Vinci nella collezione reale di pitture
Fagnart, Laure ULiege

Conference (2012, April 19)

Detailed reference viewed: 28 (3 ULiège)
See detailCopperflora.org - website
Boisson, Sylvain ULiege; Seleck, Maxime ULiege; Le Stradic, Soizig ULiege et al

Textual, factual or bibliographical database (2012)

This website is intended as a place of exchange for scientists and mine managers interested in the conservation, the restoration and the taxonomy of the copper-cobalt flora.

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See detailA sustainable method inspired by nature for the production of original bi-functional nutraceuticals containing prebiotic fibers and highly bio-available forms of calcium and magnesium
Goffin, Dorothée ULiege; Blecker, Christophe ULiege; Paquot, Michel ULiege

Poster (2012, April 18)

A sustainable method inspired by nature is proposed leading to an original bifunctional product active on gut health, metabolism regulation, immunity and mineral fortification, fighting against topical ... [more ▼]

A sustainable method inspired by nature is proposed leading to an original bifunctional product active on gut health, metabolism regulation, immunity and mineral fortification, fighting against topical chronic diseases (type2 diabetes, osteoporosis, colon cancer…). [less ▲]

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See detailInfluence of protein context on the propensity of polyglutamine tracts to induce protein aggregation into amyloid fibrils
Huynen, Céline ULiege

Poster (2012, April 18)

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of ... [more ▼]

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of the CAG codon in the corresponding genes, and are present in proteins of healthy people. They are however pathogenic when their length, due to mutations, becomes higher than a threshold generally comprised between 35 and 45Q. Such pathogenic tracts trigger the aggregation of the proteins into amyloid-like aggregates that could play an important role in the disease. It is therefore necessary to investigate at a molecular level the aggregation process of polyQ proteins. Since proteins associated with polyQ diseases are generally big and relatively insoluble, they are difficult to produce and manipulate. We have therefore decided to study the aggregating properties of polyQ proteins by designing and characterizing model proteins made of a well-characterized host protein, the β-lactamase BlaP, and polyQ tracts of different lengths (23 to 79Q) inserted at position 197 of BlaP. The aggregating properties of these BlaP chimeras recapitulate those of proteins associated with polyQ diseases. We indeed observed that there is a minimal number of glutamines (threshold) required for chimeras aggregation into amyloid-like fibrils and that the kinetics of aggregation are faster with longer glutamine repeats. Most importantly, the value of the threshold for amyloid-like fibril formation seems to critically depend on the structural integrity of BlaP and thus on the constraints applied to the polyQ tract. In the present work, we investigate more deeply the role of the protein context and the role of oligomers in the process of aggregation of BlaP chimeras. This study is mainly based on the use of two techniques: the quartz crystal microbalance with dissipation (QCM-D) in combination with atomic force microscopy (AFM), and the dynamic light scattering (DLS). Preliminary QCM-D results indicate that in native condition there is a minimal number of glutamines required for the elongation of BlaP197(Gln)79 fibrils by BlaP chimeras. This threshold is different to that observed for the aggregation monitored in solution in absence of seed. Most interestingly it corresponds to the threshold observed in denaturing conditions. These observations suggest that the conformation of BlaP is the limiting step for amyloid fibril formation by interfering with the nucleation step. Preliminary DLS experiments indicate that the oligomers formed by BlaP197(Gln)79 in native condition are on the pathway of amyloid fibril formation and could act as aggregation nuclei. [less ▲]

Detailed reference viewed: 41 (4 ULiège)
See detailInnovative multilayered nanofibers for wound dressing application
Croisier, Florence ULiege; Detrembleur, Christophe ULiege; Jérôme, Christine ULiege

Poster (2012, April 18)

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This ... [more ▼]

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This polysaccharide has shown a great potential for biomedical and pharmaceutical applications, on account of its remarkable compatibility with physiological medium. Besides, it is degraded in a physiological environment into non-toxic products, which make chitosan an outstanding candidate for short- to medium-term applications. In this respect, nanometric fibers are highly interesting as their assembly mimics the skin extracellular matrix structure. Such nanofibrous materials can be prepared by electrospinning (ESP). This technique uses a high voltage to create an electrically charged jet of polymer solution or melt which leads to fibers formation. Depending on the polymer characteristics (a.o. molecular weight, solution viscosity and conductivity) and processing conditions (electric potential, distance between syringe-capillary and collection plate, concentration, flow rate), polymer fibers ranging from nanometers to a few microns in diameter can be obtained and subsequently used as potential scaffolds, a.o. to form a temporary, artificial extracellular matrix. In the present study, electrospinning technique was combined with layer-by-layer deposition method (LBL) - a well-known method for surface coating, based on electrostatic interactions - in order to prepare multilayered chitosan-based nanofibers. The antibacterial properties of the obtained material were then assessed, and the presence of a multilayered deposit was confirmed by several techniques. (Future) possibilities for valorization: These multilayered chitosan-based nanofibers present great prospects for the preparation of new biomedical scaffolds - such as wound dressings that could improve skin regeneration. [less ▲]

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See detailPhysical Fitness and Mitochondrial Respiratory Capacity in Horse Skeletal Muscle
Votion, Dominique ULiege; Gnaiger, Erich; Lemieux, Hélène et al

in PLoS ONE (2012), 7(4), 1-12

We applied high-resolution respirometry and multiple substrate-uncoupler-inhibitor titration protocols to study mitochondrial physiology in small (1.0–2.5 mg) permeabilized muscle fibres sampled from ... [more ▼]

We applied high-resolution respirometry and multiple substrate-uncoupler-inhibitor titration protocols to study mitochondrial physiology in small (1.0–2.5 mg) permeabilized muscle fibres sampled from triceps brachii of healthy horses. The present investigation on horse skeletal muscle has tested the methodology used to study mitochondrial function in muscle microbiopsies by high-resolution respirometry to define reference protocols for horses (from sampling procedure to data analysis). [less ▲]

Detailed reference viewed: 102 (32 ULiège)
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See detailCharacterisation of metal binding affinities of HMA4 from Arabidopsis halleri
Laurent, Clémentine ULiege

Poster (2012, April 18)

Detailed reference viewed: 30 (1 ULiège)
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See detailPossible intracellular effect of the new water-soluble form of curcumin (NDS27) on the oxidant response of stimulated neutrophils
Derochette, Sandrine ULiege; Mouithys-Mickalad, Ange ULiege; Deby-Dupont, Ginette et al

Poster (2012, April 18)

Neutrophils (PMNs) are involved in host defense against infections through the production of reactive oxygen species (ROS) to kill pathologic agents. But, an excessive ROS production, called “oxidative ... [more ▼]

Neutrophils (PMNs) are involved in host defense against infections through the production of reactive oxygen species (ROS) to kill pathologic agents. But, an excessive ROS production, called “oxidative stress” is associated with tissue damages and development of chronic or acute inflammatory diseases. PMNs are prime therapeutic targets to control inflammatory events associated to ROS production. Nowadays, there is a growing interest for the use of polyphenolic molecules to modulate the inflammatory response. The aim of this work was to study the antioxidant effect of NDS27 (1), a new highly water-soluble form of the polyphenolic molecule curcumin, on in vitro stimulated equine PMNs. NDS27 (10-6 to 10-4 M) was pre-incubated with cells and eliminated before their activation. The ability of NDS27 to enter into the cells was checked by HPLC from the cellular extracts. The intracellular ROS production by phorbol myristate acetate (PMA) stimulated PMNs was measured by fluorescence using 2’,7’-dichlorofluorescin diacetate. Lucigenin dependent chemiluminescence was used to measure extracellular ROS production. Additionally, the effect of NDS27 was tested on the activity of myeloperoxidase (MPO), a hemic enzyme contributing to the oxidant response of neutrophils. The activity of the released MPO by cytochalazine B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP) stimulated PMNs was measured by SIEFED (“Specific Immunologic Extraction Followed by Enzymatic Detection”) (2). The HPLC results showed that NDS27 enters into PMNs and interacts with their membrane. NDS27 significantly and dose-dependently inhibited the ROS production in neutrophils without affecting their viability. Likewise, the activity of MPO released by PMNs was lowered by NDS27. Overall, our findings demonstrate that the membrane of neutrophils is permeable to NDS27 or interacts with the drug, suggesting that its inhibitory effect on ROS production is mainly associated to an intracellular effect probably by acting on the enzymes implied in respiratory burst like NADPH oxidase and MPO. The modulatory effect of NDS27 towards the oxidant activity of cells involved in immune and inflammatory response open therapeutic perspectives to control equine or human pathologies with excessive inflammatory reactions. 1. Neven et al. 2011, Patent Application Publication: US2011/0257126 A1 2. Serteyn et al. 2005, European Patent Specification : EP1711817 B1 [less ▲]

Detailed reference viewed: 89 (6 ULiège)
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See detailWhat is new in anemia treatment in CKD since 2010?
Krzesinski, Jean-Marie ULiege

Conference (2012, April 18)

•Partial correction (Hb in the range of 10-11.5 g/dL) of CKD-related anemia appears a safer strategy during the last 5y. So recommendation is to start ESA only when Hb <10 and to avoid too high ESA dose ... [more ▼]

•Partial correction (Hb in the range of 10-11.5 g/dL) of CKD-related anemia appears a safer strategy during the last 5y. So recommendation is to start ESA only when Hb <10 and to avoid too high ESA dose (CE DOSE study ongoing in HD)! •Identify resistant patients and try to improve it. •Importance of adequate iron management and of hepcidin role in the all-mortality risk in CKD. Newer strategies for correcting anemia are currently explored [less ▲]

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See detailClinicopathologic significance of DNA methyltransferase 1, 3a, and 3b overexpression in Tunisian breast cancers.
Ben Gacem, Riadh; Hachana, Mohamed Ridha ULiege; Ziadi, Sonia et al

in Human Pathology (2012)

DNA methyltransferase 1, 3a, and 3b affect DNA methylation, and it is thought that they play an important role in the malignant transformation of various cancers. The current study was designed to analyze ... [more ▼]

DNA methyltransferase 1, 3a, and 3b affect DNA methylation, and it is thought that they play an important role in the malignant transformation of various cancers. The current study was designed to analyze DNA methyltransferase expression by immunohistochemistry in a series of 94 Tunisian sporadic breast carcinomas. Results were correlated to clinicopathologic parameters and promoter methylation status of 8 tumor suppressor genes (BRCA1, BRCA2, RASSFA1, TIMP3, CDH1, P16, RARβ2, and DAPK). Overexpression of DNA methyltransferase 1, 3a, and 3b was detected in 46.8%, 32%, and 44.7% of cases, respectively. A significant correlation was found between DNA methyltransferase 1 overexpression and Scarff-Bloom-Richardson histologic grade III (P = .01). DNA methyltransferase 3a overexpression was significantly associated with menopausal status (P = .01), Scarff-Bloom-Richardson histologic grade III (P = .0001), estrogen (P = .04) and progesterone (P = .007) receptor negativity, and HER2 overexpression (P = .004). However, DNA methyltransferase 3a overexpression was found less frequently in the luminal A intrinsic breast cancer subtype (9.7%) than in luminal B (53%), HER2 (41%), and triple-negative (50%) subtypes (P = .001). DNA methyltransferase 3b overexpression shows significant correlation with promoter hypermethylation of BRCA1 (P = .03) and RASSFA1 (P = .04) and with the hypermethylator phenotype (more than 4 methylated genes, P = .01). These data suggest that overexpression of various DNA methyltransferases might represent a critical event responsible for the epigenetic inactivation of multiple tumor suppressor genes, leading to the development of aggressive forms of sporadic breast cancer. [less ▲]

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See detailInteractions of KLF4 and SIP1 in the regulation of E-Cadherin expression
Koopmansch, Benjamin ULiege

Poster (2012, April 18)

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See detailPrazeres do raio-x: estudo de um striptease radiográfico
Sturnack, Lionel ULiege

Conference (2012, April 18)

O striptease que propomos analisar é na verdade o título de uma obra que pertence à produção da artista italiana Benedetta Bonichi. Trata-se de uma composição mista, a meio caminho entre os raios-x e a ... [more ▼]

O striptease que propomos analisar é na verdade o título de uma obra que pertence à produção da artista italiana Benedetta Bonichi. Trata-se de uma composição mista, a meio caminho entre os raios-x e a fotografia. “Striptease” chamou a nossa atenção por várias razões. Com certeza, por seu caráter surpreendente, sendo uma cena erótica e ao mesmo tempo uma radiografia. Mas também por sua estética particular em relação aos outros artistas que desenvolvem uma técnica similar. O propósito de nossos passos é levar em consideração o lugar da obra estudada no espaço de realizações em raio-x artístico, numa perspectiva semiótica. Pretendemos desta maneira chegar à descrição fina de “Striptease, por meio do estabelecimento de um conjunto de formantes plásticos estáveis funcionando como base sobre a qual se correlacionam e se diferenciam os raios-x artísticos estudados. Por isso, desenvolveremos o estudo segundo três etapas gerais. Com a primeira, tentaremos efetuar uma descrição de “Striptease” segundo os três eixos da leitura plástica padrão, tal como foram trabalhados por Jean-Marie Floch: cromático, eidético e topológico. Na segunda etapa, adotaremos o mesmo ponto de vista para a descrição plástica de cinco obras de quatro outros artistas. O interesse da segunda parte consiste em ver como se atualizam as unidades plásticas em cada realização particular, a fim de observar as suas possíveis convergências e divergências. Na última etapa, poderemos considerar a estética de cada obra à luz das atualizações plásticas e das integrações entre elas com outros patamares temáticos. Conseguiremos, finalmente, entender a pertinência e a particularidade de “Striptease” como obra com vocação reflexiva a respeito da prática do raio-x artístico. [less ▲]

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See detailOptimization of recombinant root-secreted IgGs production in Arabidopsis thaliana by screening cell wall mutants
Boulanger, Benoit ULiege; Périlleux, Claire ULiege; Tocquin, Pierre ULiege

Poster (2012, April 18)

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by ... [more ▼]

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by human pathogens. mAbs are glycoproteins that require to be targeted to the plant secretory pathway in order to be properly folded and matured. They are ultimately delivered in the cell wall and are expected to be freely released in the extracellular space and the external medium, which would greatly simplify downstream processing. However, a significant part of plant produced and secreted mAbs remains bound to the cell wall, therefore hindering recovery. In this study, we evaluated the extra-cellular release of root-secreted proteins of wild-type plants and cell wall mutants of Arabidopsis thaliana. Recovered protein were either analyzed by SDS-PAGE for full proteome profiling or by gelatin zymography to reveal the activity of cell wall-bound proteases. The production, secretion and release of recombinant IgG will be eventually studied in transgenic hairy-roots generated from selected mutants. [less ▲]

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See detailOptimisation of new biodegradable microcarriers tailored for tissue engineering
Demina, T; Akopova, T; Tsoy, A et al

Poster (2012, April 18)

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See detailNeuropeptides and endogenous opioids expression during silent chronic inflammation
Chavarria Bolanos, D; de Jesus Pozos Guillén, A; Grandfils, Christian ULiege et al

Poster (2012, April 18)

Detailed reference viewed: 13 (2 ULiège)
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See detail1-84 PTH and KDIGO Guidelines
CAVALIER, Etienne ULiege

Conference (2012, April 18)

Detailed reference viewed: 223 (5 ULiège)