Du diagnostic à l'intervention en entreprise: quelle réalité, quelles limites?
Conference (2012, April 17)Detailed reference viewed: 37 (5 ULg)
THE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION
Shafiei, Rasoul ; Thonart, Philippe
in THE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION (2012, April 17)
Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular ... [more ▼]
Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular functions of acetic acid bacteria. In previous studies in CWBI, Acetobacter senegalensis was used for production of dried vinegar starters; however the impact of stressors (ethanol and acetic acid) on A. senegalensis remained unclear. In this study, different techniques such as flow cytometry, culturability on solid medium and 2-DiGE were used comparatively to investigate the effect of carbon sources of inoculum media on the tolerance of A. senegalensis to stressors. Analysis of respiration system by flow cytometric methods showed that the presence of 2% (v/v) acetic acid in inoculum medium, in one hand, causes 80% of cells to continue to do respiration after a sudden exposure to 1- 3% (v/v) acetic acid in stress media while 89.7% of cells grown in glucose appeared as dead cells after an abrupt exposure to 3%(v/v) of acetic acid. On the other hand, 59.2% and 49.33% of cells grown in the presence of 2% (v/v) of acetic acid could maintain their entire membrane integrity after exposure to 1% and 3% (v/v) of acetic acid, respectively. Inoculum medium contained 5% (v/v) of ethanol as a carbon source enabled about 90% of cells to keep their growing capacities after a sudden exposure to 3% acetic acid. In contrast, just 40% of cells grown in glucose as a carbon source maintained their culturability on solid medium after exposure to 1% acetic acid. A similar profile of culturability was observed for the cells grown in 5% (v/v) ethanol or 2% (v/v) of acetic acid. A proteomic approach (2-DiGE) was used to analyze proteins expressed in the presence of different carbon sources. Differentially expressed proteins were mainly associated with energy metabolism, carbohydrate metabolisms, folding, sorting and degradation processes. The relative abundance of proteins was extensively different for cell grown in glucose compared with protein contents of cells grown in ethanol or acetic acid. In conclusion, production of a cost effective vinegar starter needs a qualified biomass which tolerates ethanol and acetic acid. Tolerance of A. senegalensis to acetic acid depends to a great extent on the composition of the medium which cells grow in. In spite of low adaption to acetic acid for cell grown in glucose, using ethanol or acetic acid in inoculum media renders a physiological state in A. senegelensis which enables it to cope with higher concentration of acetic acid readily, this biomass has a potential to be used as a starter. [less ▲]Detailed reference viewed: 46 (8 ULg)
Interview à RTL - Radio sur le thème "Les 50 ans de la PAC"
Diverse speeche and writing (2012)Detailed reference viewed: 22 (4 ULg)
Traffic Situation Assessment and Intervention Strategy of a Collision Avoidance System based on Galileo Satellite Positioning
Christen, Fréderic ; ; et al
E-print/Working paper (2012)
Nowadays, collision avoidance systems (CAS) are an intensive research topic since the majority of all traffic accidents are collisions that are caused due to inattention or unadjusted driving behavior of ... [more ▼]
Nowadays, collision avoidance systems (CAS) are an intensive research topic since the majority of all traffic accidents are collisions that are caused due to inattention or unadjusted driving behavior of the driver. Up to date prototypic CAS are based on on-board environmental sensors, such as camera or radar systems, that scan the vehicle's surrounding environment in order to assess the situation's hazardousness. The functionality of the used sensors under varying environmental conditions and the limited sensor covering area require an enormous effort to ensure a reliable detection of obstacles, and thus limit the application of the systems. In order to expand the operating field of such systems, a Galileo-based CAS will be developed within the project ‘Galileo above’ (application centre for ground based traffic). This advanced driver assistance system (ADAS) detects surrounding vehicles that are on collision course and reacts autonomously, if the driver does not intervene to avoid the crash. For this purpose the system initiates an emergency stop and/or an emergency steering maneuver. For the development of the CAS the Galileo test centre automotiveGATE in Aldenhoven, Germany will be used. On this test area pseudolites will be installed which provide Galileo-like navigation signals. Thus, the development respectively tuning of Galileo-based vehicle systems will be enabled, so as to have them available on the market when the Galileo satellite system reaches its full operational capability (FOC). The focus of this paper is on the traffic situation assessment and intervention strategy of the CAS. This includes the perception and analysis of the driving situation, the detection of potential collision situation, the definition of an adequate system reaction and the planning of an evasion trajectory. Furthermore, an outlook on the model predictive control for longitudinal and/or lateral control (braking and/or steering maneuver) will be presented. [less ▲]Detailed reference viewed: 49 (14 ULg)
Temps et politique. Suivre les énoncés temporels associés aux visions de l’avenir au départ de la mise à l’agenda politique climatique belge
Scientific conference (2012, April 16)Detailed reference viewed: 16 (2 ULg)
The inseminating bull and plasma pregnancy-associated glycoprotein levels were related to peripheral leukocyte counts during the late pregnancy/early postpartum period in high-producing dairy cows.
; ; et al
in Theriogenology (2012), 77(7), 1390-1397
The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the ... [more ▼]
The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the subsequent productive and reproductive performance of the animal. At parturition, a drop in the immune functions of the peripheral blood polymorph-nuclear cells (PMN) was observed after a peak of Pregnancy-Associated Glycoproteins (PAGs) and recently, the inseminating bull was found to affect the plasma levels of bovine Pregnancy-Associated Glycoproteins (bovine PAGs) in the pregnant Holstein-Friesian dairy cows. The present work was aimed to determine whether changes in leukocyte counts during the peripartum, indicative of the animal immune status, could be related to the inseminating bull and to the levels of plasma pregnancy-associated glycoproteins (PAGs). Ninety six clinically healthy, single pregnant cows were selected in a commercial dairy herd. Six blood samples (four prepartum and two postpartum) were obtained every two weeks, and total and differential blood cell counts were analyzed. Based on procedures of General Linear Model analysis of variance for repeated measures, the inseminating bull affected counts of total leukocytes and lymphocytes (P<0.001; between-subject effects) throughout the peripartum period. In addition, cows with high plasma levels of PAGs (>900 ρg/µL) on Day 262-268 of gestation had higher numbers of total leukocytes and neutrophils throughout the peripartum period (P<0.001; between-subject effects). Young animals (≤ 1 lactation) had higher total leukocyte and lymphocytes counts than older cows (two or more lactations) throughout the study period. These results showed a clear relationship between the inseminating bull and plasma PAG levels and peripheral leukocyte counts during the peripartum period of dairy cows. [less ▲]Detailed reference viewed: 42 (5 ULg)
How does engagement in reading predict digital reading proficiency among 15 years-olds ?
Baye, Ariane ; Lafontaine, Dominique ; Monseur, Christian
Conference (2012, April 15)Detailed reference viewed: 11 (0 ULg)
Exploring Gender Variability and Performance at the Extreme Tails of the Distribution in Reading
Monseur, Christian ; Baye, Ariane
Conference (2012, April 15)Detailed reference viewed: 16 (3 ULg)
Learning Latin: Grammars and Annotations in Latin Papyri
Scappaticcio, Maria Chiara
Conference (2012, April 14)Detailed reference viewed: 26 (1 ULg)
Corporéité et politique chez Deleuze : continuité et littéralité autour du corps sans organes
Conference (2012, April 13)Detailed reference viewed: 110 (14 ULg)
Collective bargaining on occupational health: coal worker’s pneumoconiosis in Belgium (c.1937-c.1970)
Conference (2012, April 13)Detailed reference viewed: 19 (0 ULg)
The new institutional settlement in EU financial regulation: constitutionalizing regulatory coopetition?
Van Cleynenbreugel, Pieter
Scientific conference (2012, April 13)
I presented a part of my doctoral research at this U.S. doctoral conference.Detailed reference viewed: 15 (2 ULg)
participation politique, diaspora congolaise et transnationalismes
Demart, Sarah ;
Conference (2012, April 13)Detailed reference viewed: 12 (1 ULg)
migrations congolaises en Belgique et géographies religieuses
Demart, Sarah ; Melice, Anne ; Meiers, Bénédicte
Conference (2012, April 13)Detailed reference viewed: 34 (1 ULg)
Présidence de la session "Literary Monsters" - colloque international "Monsters : Subject, Object, Abject"
Diverse speeche and writing (2012)Detailed reference viewed: 17 (0 ULg)
Coupling Cell Mechanics and Intracellular Signalling: Mechanotransduction through ERK
Kerkhofs, Johan ; Geris, Liesbet ; et al
in Middleton, John (Ed.) The Proceedings of the 10th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering. Hotel Berlin, Berlin, Germany, April 7th – 11th, 2012 pages:0-0 (2012, April 12)Detailed reference viewed: 15 (1 ULg)