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See detailDidactique et épistémologie : mariage d'amour ou mariage forcé ?
Schneider-Gilot, Marguerite ULiege

Scientific conference (2012, April 20)

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See detailDBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation
Close, Pierre ULiege; East, Phil; Svejstrup, Barbara et al

in Nature (2012)

Alternative messenger RNA splicing is the main reason that vast mammalian proteomic complexity can be achieved with a limited number of genes. Splicing is physically and functionally coupled to ... [more ▼]

Alternative messenger RNA splicing is the main reason that vast mammalian proteomic complexity can be achieved with a limited number of genes. Splicing is physically and functionally coupled to transcription, and is greatly affected by the rate of transcript elongation1–3. As the nascent pre-mRNA emerges from transcribing RNA polymerase II (RNAPII), it is assembled into a messenger ribonucleoprotein (mRNP) particle; this is the functional form of the nascent pre-mRNA and determines the fate of the mature transcript4. However, factors that connect the transcribing polymerase with the mRNP particle and help to integrate transcript elongation with mRNA splicing remain unclear. Here we characterize the human interactome of chromatin-associated mRNP particles. This led us to identify deleted in breast cancer 1 (DBC1) and ZNF326 (which we call ZNF-protein interacting with nuclear mRNPs and DBC1 (ZIRD)) as subunits of a novel protein complex—named DBIRD—that binds directly to RNAPII. DBIRD regulates alternative splicing of a large set of exons embedded in (A 1 T)-rich DNA, and is present at the affected exons. RNAinterference- mediated DBIRD depletion results in region-specific decreases in transcript elongation, particularly across areas encompassing affected exons. Together, these data indicate that the DBIRD complex acts at the interface between mRNP particles and RNAPII, integrating transcript elongation with the regulation of alternative splicing. [less ▲]

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See detailCrise de la socialisation au sein d'une Société du travail sur Soi
Vrancken, Didier ULiege

Conference (2012, April 19)

Ainsi que l’ont bien identifié les organisateurs de ce colloque, il est difficile d’évoquer aujourd’hui le thème de la socialisation en sociologie sans lui accoler le terme de crise. A telle enseigne que ... [more ▼]

Ainsi que l’ont bien identifié les organisateurs de ce colloque, il est difficile d’évoquer aujourd’hui le thème de la socialisation en sociologie sans lui accoler le terme de crise. A telle enseigne que l’on peut avancer qu’une réelle lecture « déclinologique » s’est répandue dans de nombreux pays européens et en particulier dans le domaine de la sociologie francophone. Ainsi cette crise de la socialisation va-t-elle souvent de pair avec la montée de l’individualisme, se déclinant notamment autour des thèmes du « retour de l’acteur », de la montée du nouvel « individualisme », de l’émergence du « sujet », de la « singularité », de la « subjectivation », etc. Tous ces thèmes laissant de plus en plus entrapercevoir un individu autonome mais surchargé par le poids des responsabilités. Un individu mis à l’épreuve (de la vie, des autres, de lui-même), un individu tenu de miser sur ses capacités mais en même temps saisi par le doute, celui d’être continuellement à la hauteur, d’« être capable ». De plus en plus d’études en sciences humaines et sociales se sont penchées sur la face sombre de cette évolution, celle de l’émergence de pathologies du lien social telles que les addictions, la dépression, le harcèlement moral, la souffrance sociale et ses multiples dimensions : souffrance au travail face à l’organisation ; précarité sociale ou encore appel constant à la subjectivité pour s’affirmer. Dans cette optique, la crise est alors perçue comme un affaiblissement du lien social en regard des sociétés qui nous ont précédés. Cette assertion sera pleinement discutée dans le cadre de cette conférence. [less ▲]

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See detailVictimes d'abus sexuels et trajectoires délinquantes: exploration des tuteurs de résilience
Buzitu, Rachel ULiege

Scientific conference (2012, April 19)

This study is concerning the resilience related a juvenile delinquency among victims of sexual abuse: sexual abuse has been recognized as a powerful risk factor for the emergence of deviant and criminal ... [more ▼]

This study is concerning the resilience related a juvenile delinquency among victims of sexual abuse: sexual abuse has been recognized as a powerful risk factor for the emergence of deviant and criminal.behavior. Our study aims is to explore and highlight the protective factors that prevents victims of sexual abuse to engage in delinquent trajectory. The sample consists of teenage girls who have revealed at minimum last year, their intergenerational abuse position. These girls, are all between 12 and 18 years old,and divided into sub-groups: victims not offenders and victims offenders (self revealed and judicial control). Each teenage victims is compared to a normative population on different psychological’s functioning dimensions (aggression, self-esteem and pubertal timing). A comparative analysis between victims and not offenders is conducted to determine the elements that have a protective role. Clinical tests also are performed and linked to data from comparative analysis. The main factors of resilience (compared to a juvenile delinquency commitment) highlights concern the individual, the family system and the natural environment. [less ▲]

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See detailL’inflammation induite par les acides gras libres
L'Homme, Laurent ULiege

Conference (2012, April 19)

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See detailAdvances in biomaterials to repair cartilage
Maquet, Véronique; Chausson, Mickael; Gautier, Sandrine et al

Conference (2012, April 19)

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See detailAn original methodology to study the synovial tissue in OA patient
Lambert, Cécile ULiege; Dubuc, Jean-Emile; Henrotin, Yves ULiege

Poster (2012, April 19)

Purpose: Synovial membrane plays a key role in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. Using an original methodology comparing normal/reactive ... [more ▼]

Purpose: Synovial membrane plays a key role in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. Using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membrane areas from the same OA patient, we investigated the crosstalk between inflammation and angiogenesis. We also analyzed the gene expression pattern of synovial cells from these different areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according the macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and the biopsies from N/R and I areas cultured separately for a period of 7 days. Inflammatory and angiogenic mediators were evaluated in the culture supernatant by immunoassays (ELISA) or visualized by immunohistochemistry. Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format human HT-12 BeadChip (Illumina Inc.). Results: Immunohistochemistry showed an increase of lymphocyte infiltration, vascular density and VEGF expression in I compared N/R synovial biopsies. Synovial cells from I areas produced more IL-6, IL-8 and VEGF but less TSP-1 than cells isolated from N/R synovial biopsies. By microarray analysis, 896 differentially expressed genes between N/R and I zones were identified. Among them, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR  0.75). A significant number of the top ranking differentially expressed genes were identified as inflammatory, cartilage metabolism, Wnt or angiogenic pathways. Conclusion: In this study, we have demonstrated the pro-inflammatory and pro-angiogeneic status of I area of the OA synovial membrane. Using a unique culture system, this study is the first to identify different expression pattern between two areas of the synovial membrane in the same OA patient. This original methodology could be further used to go deeper into the knowledge of the role of synovial membrane in OA. Possibilities for valorization: This analysis provided interesting information regarding new potent intermediates that could be potential new targets for the diagnosis or treatment of OA. [less ▲]

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See detailOSS 117. Le monde comme terrain de jeu
Dozo, Björn-Olav ULiege

Conference given outside the academic context (2012)

Detailed reference viewed: 21 (2 ULiège)
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See detailEncéphalopathie anoxo-ischémique et neuroprotection par hypothermie contrôlée
VIELLEVOYE, Renaud ULiege

Conference (2012, April 19)

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See detailMethod for producing antibody using "naked" expression vector expressing type II transmembrane fusion protein
Renauld, Jean Christophe; Lemaire, Muriel; Dumoutier, Laurie et al

Patent (2012)

Detailed reference viewed: 42 (4 ULiège)
See detailLeonardo da Vinci nella collezione reale di pitture
Fagnart, Laure ULiege

Conference (2012, April 19)

Detailed reference viewed: 28 (3 ULiège)
See detailCopperflora.org - website
Boisson, Sylvain ULiege; Seleck, Maxime ULiege; Le Stradic, Soizig ULiege et al

Textual, factual or bibliographical database (2012)

This website is intended as a place of exchange for scientists and mine managers interested in the conservation, the restoration and the taxonomy of the copper-cobalt flora.

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See detailA sustainable method inspired by nature for the production of original bi-functional nutraceuticals containing prebiotic fibers and highly bio-available forms of calcium and magnesium
Goffin, Dorothée ULiege; Blecker, Christophe ULiege; Paquot, Michel ULiege

Poster (2012, April 18)

A sustainable method inspired by nature is proposed leading to an original bifunctional product active on gut health, metabolism regulation, immunity and mineral fortification, fighting against topical ... [more ▼]

A sustainable method inspired by nature is proposed leading to an original bifunctional product active on gut health, metabolism regulation, immunity and mineral fortification, fighting against topical chronic diseases (type2 diabetes, osteoporosis, colon cancer…). [less ▲]

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See detailInfluence of protein context on the propensity of polyglutamine tracts to induce protein aggregation into amyloid fibrils
Huynen, Céline ULiege

Poster (2012, April 18)

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of ... [more ▼]

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of the CAG codon in the corresponding genes, and are present in proteins of healthy people. They are however pathogenic when their length, due to mutations, becomes higher than a threshold generally comprised between 35 and 45Q. Such pathogenic tracts trigger the aggregation of the proteins into amyloid-like aggregates that could play an important role in the disease. It is therefore necessary to investigate at a molecular level the aggregation process of polyQ proteins. Since proteins associated with polyQ diseases are generally big and relatively insoluble, they are difficult to produce and manipulate. We have therefore decided to study the aggregating properties of polyQ proteins by designing and characterizing model proteins made of a well-characterized host protein, the β-lactamase BlaP, and polyQ tracts of different lengths (23 to 79Q) inserted at position 197 of BlaP. The aggregating properties of these BlaP chimeras recapitulate those of proteins associated with polyQ diseases. We indeed observed that there is a minimal number of glutamines (threshold) required for chimeras aggregation into amyloid-like fibrils and that the kinetics of aggregation are faster with longer glutamine repeats. Most importantly, the value of the threshold for amyloid-like fibril formation seems to critically depend on the structural integrity of BlaP and thus on the constraints applied to the polyQ tract. In the present work, we investigate more deeply the role of the protein context and the role of oligomers in the process of aggregation of BlaP chimeras. This study is mainly based on the use of two techniques: the quartz crystal microbalance with dissipation (QCM-D) in combination with atomic force microscopy (AFM), and the dynamic light scattering (DLS). Preliminary QCM-D results indicate that in native condition there is a minimal number of glutamines required for the elongation of BlaP197(Gln)79 fibrils by BlaP chimeras. This threshold is different to that observed for the aggregation monitored in solution in absence of seed. Most interestingly it corresponds to the threshold observed in denaturing conditions. These observations suggest that the conformation of BlaP is the limiting step for amyloid fibril formation by interfering with the nucleation step. Preliminary DLS experiments indicate that the oligomers formed by BlaP197(Gln)79 in native condition are on the pathway of amyloid fibril formation and could act as aggregation nuclei. [less ▲]

Detailed reference viewed: 41 (4 ULiège)