References of "2012"
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See detailLeonardo da Vinci nella collezione reale di pitture
Fagnart, Laure ULg

Conference (2012, April 19)

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See detailMethod for producing antibody using "naked" expression vector expressing type II transmembrane fusion protein
Renauld, Jean Christophe; Lemaire, Muriel; Dumoutier, Laurie et al

Patent (2012)

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See detailDBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation
Close, Pierre ULg; East, Phil; Svejstrup, Barbara et al

in Nature (2012)

Alternative messenger RNA splicing is the main reason that vast mammalian proteomic complexity can be achieved with a limited number of genes. Splicing is physically and functionally coupled to ... [more ▼]

Alternative messenger RNA splicing is the main reason that vast mammalian proteomic complexity can be achieved with a limited number of genes. Splicing is physically and functionally coupled to transcription, and is greatly affected by the rate of transcript elongation1–3. As the nascent pre-mRNA emerges from transcribing RNA polymerase II (RNAPII), it is assembled into a messenger ribonucleoprotein (mRNP) particle; this is the functional form of the nascent pre-mRNA and determines the fate of the mature transcript4. However, factors that connect the transcribing polymerase with the mRNP particle and help to integrate transcript elongation with mRNA splicing remain unclear. Here we characterize the human interactome of chromatin-associated mRNP particles. This led us to identify deleted in breast cancer 1 (DBC1) and ZNF326 (which we call ZNF-protein interacting with nuclear mRNPs and DBC1 (ZIRD)) as subunits of a novel protein complex—named DBIRD—that binds directly to RNAPII. DBIRD regulates alternative splicing of a large set of exons embedded in (A 1 T)-rich DNA, and is present at the affected exons. RNAinterference- mediated DBIRD depletion results in region-specific decreases in transcript elongation, particularly across areas encompassing affected exons. Together, these data indicate that the DBIRD complex acts at the interface between mRNP particles and RNAPII, integrating transcript elongation with the regulation of alternative splicing. [less ▲]

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See detailCopperflora.org - website
Boisson, Sylvain ULg; Seleck, Maxime ULg; Le Stradic, Soizig ULg et al

Textual, factual or bibliographical database (2012)

This website is intended as a place of exchange for scientists and mine managers interested in the conservation, the restoration and the taxonomy of the copper-cobalt flora.

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See detailAdvances in biomaterials to repair cartilage
Maquet, Véronique; Chausson, Mickael; Gautier, Sandrine et al

Conference (2012, April 19)

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See detailAn original methodology to study the synovial tissue in OA patient
Lambert, Cécile ULg; Dubuc, Jean-Emile; Henrotin, Yves ULg

Poster (2012, April 19)

Purpose: Synovial membrane plays a key role in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. Using an original methodology comparing normal/reactive ... [more ▼]

Purpose: Synovial membrane plays a key role in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. Using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membrane areas from the same OA patient, we investigated the crosstalk between inflammation and angiogenesis. We also analyzed the gene expression pattern of synovial cells from these different areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according the macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and the biopsies from N/R and I areas cultured separately for a period of 7 days. Inflammatory and angiogenic mediators were evaluated in the culture supernatant by immunoassays (ELISA) or visualized by immunohistochemistry. Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format human HT-12 BeadChip (Illumina Inc.). Results: Immunohistochemistry showed an increase of lymphocyte infiltration, vascular density and VEGF expression in I compared N/R synovial biopsies. Synovial cells from I areas produced more IL-6, IL-8 and VEGF but less TSP-1 than cells isolated from N/R synovial biopsies. By microarray analysis, 896 differentially expressed genes between N/R and I zones were identified. Among them, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR  0.75). A significant number of the top ranking differentially expressed genes were identified as inflammatory, cartilage metabolism, Wnt or angiogenic pathways. Conclusion: In this study, we have demonstrated the pro-inflammatory and pro-angiogeneic status of I area of the OA synovial membrane. Using a unique culture system, this study is the first to identify different expression pattern between two areas of the synovial membrane in the same OA patient. This original methodology could be further used to go deeper into the knowledge of the role of synovial membrane in OA. Possibilities for valorization: This analysis provided interesting information regarding new potent intermediates that could be potential new targets for the diagnosis or treatment of OA. [less ▲]

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See detailHemocompatibility of nanocarriers designed to transport biopharmaceutical drugs
Sevrin, Chantal ULg; Cerda, Bernardino; Lombart, François ULg et al

Poster (2012, April 18)

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed ... [more ▼]

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed the large surface they are able to expose to the biological environment promotes their interaction with various biochemicals, in particular proteins which can after adsorption elicit the activation of biological cascades either responsible from NP clearance or/and harmful body reaction (inflammatory / coagulation). In the frame of the European Integrated Project : “Nanobiopharmaceutics”, we have the opportunity to compare the hemoreactivity of about 145 different NP samples differing in core and surface chemistry and classified according to their expected difference in hydrophobicity based on the nature of their core materials. According to this classification, PLGA nanoparticles, polyglycidol-polyethyethylene oxide nanoparticles, polyglycidol thyolated or polyacrylamide nanogels, and polyelectrolyte complexes either based on polyamidoamine or poly(N,N-dimethylamino-2-ethylmethacrylate) have been evaluated within a concentration ranging from 0.3 to 1000 =g/mL. These in vitro tests have been realized for screening purpose adopting normal human bloods and according to Iso 10993. As a summary of this extensive study, our results clearly highlight that most of the polymeric nanoparticles evaluated give rise to some alterations of the blood components. In particular the platelets, intrinsic pathway of coagulation and complement activation are the most reactive biological parameters in the presence of these nanostuctures. Although not strictly related to the surface chemistry our classification has also allowed us to derive some clear correlations between nanomaterial properties and their hemoreactivity. Within the class of polyelectrolyte electrolyte complexes, the modifications brought in the surface chemistry has drastically improved their hemoreactivity. [less ▲]

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See detailPrazeres do raio-x: estudo de um striptease radiográfico
Sturnack, Lionel ULg

Conference (2012, April 18)

O striptease que propomos analisar é na verdade o título de uma obra que pertence à produção da artista italiana Benedetta Bonichi. Trata-se de uma composição mista, a meio caminho entre os raios-x e a ... [more ▼]

O striptease que propomos analisar é na verdade o título de uma obra que pertence à produção da artista italiana Benedetta Bonichi. Trata-se de uma composição mista, a meio caminho entre os raios-x e a fotografia. “Striptease” chamou a nossa atenção por várias razões. Com certeza, por seu caráter surpreendente, sendo uma cena erótica e ao mesmo tempo uma radiografia. Mas também por sua estética particular em relação aos outros artistas que desenvolvem uma técnica similar. O propósito de nossos passos é levar em consideração o lugar da obra estudada no espaço de realizações em raio-x artístico, numa perspectiva semiótica. Pretendemos desta maneira chegar à descrição fina de “Striptease, por meio do estabelecimento de um conjunto de formantes plásticos estáveis funcionando como base sobre a qual se correlacionam e se diferenciam os raios-x artísticos estudados. Por isso, desenvolveremos o estudo segundo três etapas gerais. Com a primeira, tentaremos efetuar uma descrição de “Striptease” segundo os três eixos da leitura plástica padrão, tal como foram trabalhados por Jean-Marie Floch: cromático, eidético e topológico. Na segunda etapa, adotaremos o mesmo ponto de vista para a descrição plástica de cinco obras de quatro outros artistas. O interesse da segunda parte consiste em ver como se atualizam as unidades plásticas em cada realização particular, a fim de observar as suas possíveis convergências e divergências. Na última etapa, poderemos considerar a estética de cada obra à luz das atualizações plásticas e das integrações entre elas com outros patamares temáticos. Conseguiremos, finalmente, entender a pertinência e a particularidade de “Striptease” como obra com vocação reflexiva a respeito da prática do raio-x artístico. [less ▲]

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See detailPsychrotrophic and psychrophylic Clostridium responsible for meat spoilage
Rodrigues, A.; Taminiau, Bernard ULg; Daube, Georges ULg

Poster (2012, April 18)

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See detailOptimization of recombinant root-secreted IgGs production in Arabidopsis thaliana by screening cell wall mutants
Boulanger, Benoit ULg; Périlleux, Claire ULg; Tocquin, Pierre ULg

Poster (2012, April 18)

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by ... [more ▼]

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by human pathogens. mAbs are glycoproteins that require to be targeted to the plant secretory pathway in order to be properly folded and matured. They are ultimately delivered in the cell wall and are expected to be freely released in the extracellular space and the external medium, which would greatly simplify downstream processing. However, a significant part of plant produced and secreted mAbs remains bound to the cell wall, therefore hindering recovery. In this study, we evaluated the extra-cellular release of root-secreted proteins of wild-type plants and cell wall mutants of Arabidopsis thaliana. Recovered protein were either analyzed by SDS-PAGE for full proteome profiling or by gelatin zymography to reveal the activity of cell wall-bound proteases. The production, secretion and release of recombinant IgG will be eventually studied in transgenic hairy-roots generated from selected mutants. [less ▲]

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See detailNew alginate-chitosan hydrogel to repair cartilage
Henrotin, Yves ULg

Conference (2012, April 18)

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See detail1-84 PTH and KDIGO Guidelines
CAVALIER, Etienne ULg

Conference (2012, April 18)

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See detailReading an Arthurian Prose Romance through its Textual Tradition.
Morato, Nicola ULg

Scientific conference (2012, April 18)

Detailed reference viewed: 5 (1 ULg)