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See detailExpression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates
Lambert, Cécile ULg; Dubuc, Jean-Emile; Hennuy, Benoît ULg et al

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. <br />Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. <br />Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. <br />Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

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See detailThe earthquake sedimentary record in marine sediment from cores in the western part of the Marmara Sea, Turkey
Drab, Laureen; Hubert, Aurelia ULg; Schmidt, Sabine et al

Conference (2012, April)

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See detailL'astronomie dans le monde
Manfroid, Jean ULg

Article for general public (2012)

Collision dans le Dragon; Trou noir de masse intermédiaire;Zoom sur une galaxie lointaine; Les jours de Vénus s’allongent ?; Lumière cendrée; Anomalies magnétiques lunaires; Océan martien; Hercule par le ... [more ▼]

Collision dans le Dragon; Trou noir de masse intermédiaire;Zoom sur une galaxie lointaine; Les jours de Vénus s’allongent ?; Lumière cendrée; Anomalies magnétiques lunaires; Océan martien; Hercule par le VST [less ▲]

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See detailUnderstanding of the Venus upper atmosphere dynamics with O2(a1 ) Venus Express observations
Soret, Lauriane ULg; Gérard, Jean-Claude ULg; Piccioni, Giuseppe et al

Poster (2012, April)

The O2(a1 ) nightglow emission at 1.27 m may be used as a tracer of the dynamics prevailing in the Venusian upper mesosphere. This emission has thus been observed with ground-based telescopes and from ... [more ▼]

The O2(a1 ) nightglow emission at 1.27 m may be used as a tracer of the dynamics prevailing in the Venusian upper mesosphere. This emission has thus been observed with ground-based telescopes and from space with instruments such as VIRTIS on board Venus Express. Observations have shown that the emission maximum is statistically located close to the antisolar point at 96 km. As originally suggested by Connes et al. (1979), such an emission results from the production of oxygen atoms on the Venus dayside by photodissociation and electron impact dissociation of CO2 and CO, which are then transported to the nightside by the subsolar to antisolar general circulation, where they recombine to create excited O2(a1 ) molecules. Their radiative deexcitation produces the O2(a1 ) nightglow with a maximum near the antisolar point. However, VIRTIS observations indicate that the O2(a1 ) nightglow emission is highly variable, both in intensity and location. Actually, when considering individual observations, the patch of bright emission is rarely located at the antisolar point and the brighter area around this point is the result of statics accumulation. Also, when considering several individual observations acquired in a short period of time, it is possible to follow an individual emission patch and to deduce its displacement and its brightness variation due to activation or deactivation. In this study, we analyze several sequences of VIRTIS observations in order to understand the Venus upper mesosphere dynamics.We show that the intensity can vary by several megaRayleighs in a couple of hours with effective lifetimes on the order of several hours. The horizontal motion of the spots leads to the conclusion that winds in the 95-100 km region are in the range of 25 to 150 m s-1, in good agreement with the study by Hueso et al. (2008). [less ▲]

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See detailOrganizational identification and commitment: The impact of POS and prestige
Marique, Géraldine; Stinglhamber, Florence; Desmette, Donatienne et al

Poster (2012, April)

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See detailEarly Jezirah 3b Pottery from Tell Beydar. Selected Inventories from latest excavation campaigns
Mas, Juliette ULg; Gavagnin, Katia

Conference (2012, April)

Since 1992, excavations at Tell Beydar have offered the opportunity to explore an urban centre of the Upper Khabur region. The extensive exploration of the EJZ 3b levels gave us access to the urbanism and ... [more ▼]

Since 1992, excavations at Tell Beydar have offered the opportunity to explore an urban centre of the Upper Khabur region. The extensive exploration of the EJZ 3b levels gave us access to the urbanism and the architecture of the city as well as its material culture, especially the pottery. The assemblages presented in this paper were exclusively found directly on the floors of various buildings (of a public, domestic, religious, offi- cial nature) and inside a grave. Most of these inventories consist of complete vessels discovered either intact or smashed but restorable. The aim of this paper is to investigate if, or to what extent, the intra-site distribution of the morphological repertoire of Beydar IIIb ceramic is related to its context of use. [less ▲]

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See detailWhen should we measure vitamin D concentration in clinical pratice?
Souberbielle, Jean-Claude; Courbebaisse, Marie; Cormier, Catherine et al

in Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum (2012), 72(suppl 243), 129-135

The many recently published data on vitamin D have raised much interest in the medical community. One of the consequences has been a great increase in the prescription of vitamin D concentration ... [more ▼]

The many recently published data on vitamin D have raised much interest in the medical community. One of the consequences has been a great increase in the prescription of vitamin D concentration measurements in clinical practice. It must be reminded that only the measurement of 25-hydroxyvitamin D (25(OH)D) concentration is indicated to evaluate vitamin D status. Furthermore, since vitamin D insuffi ciency is so common, since treatment is inexpensive and has a large safety margin, and since we already have much data suggesting that besides its classic effects on bone and mineral metabolism, vitamin D may potentially be helpful for the prevention/management of several diseases, perhaps should it be prescribed to everyone without prior testing? In our opinion, there are however groups of patients in whom estimation of vitamin D status is legitimate and may be recommended. This includes patients in whom a “ reasonably ” evidence-based target concentration (i.e., based on randomized clinical trials when possible) should be achieved and/or maintained such as patients with rickets/osteomalacia, osteoporosis, chronic kidney disease and kidney transplant recipients, malabsorption, primary hyperparathyroidism, granulomatous disease, and those receiving treatments potentially inducing bone loss. Other patients in whom vitamin D concentration may be measured are those with symptoms compatible with a severe vitamin D defi ciency or excess persisting without explanation such as those with diffuse pain, or elderly individuals who fall, or those receiving treatments which modify vitamin D metabolism such as some anti-convulsants. Measurement of Vitamin D concentrations should also be part of any exploration of calcium/phosphorus metabolism which includes measurement of serum calcium, phosphate and PTH. [less ▲]

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See detailLes régimes matrimoniaux en droit international privé - autour de trois questions d'actualité
Wautelet, Patrick ULg

in Revue Droit International Privé = Tijdschrift voor Internationaal Privaatrecht (2012), 11(1), 29-43

Ce papier fait le point sur trois questions d'actualité dans la pratique du droit international privé des régimes matrimoniaux. Ces questions ont été sélectionnées parce qu'elles peuvent également se ... [more ▼]

Ce papier fait le point sur trois questions d'actualité dans la pratique du droit international privé des régimes matrimoniaux. Ces questions ont été sélectionnées parce qu'elles peuvent également se poser dans d'autres domaines du droit international privé : il s'agit d'abord de la distinction entre questions de forme et questions de fond, ensuite de l'appréhension et du fonctionnement des règles de rattachement fondée sur une échelle de rattachement et enfin de l'application concrète de l'exception d'ordre public [less ▲]

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See detailL'économie sociale et solidaire
Huybrechts, Benjamin ULg

in Blanchet, Vivien; Carimentrand, Aurélie (Eds.) Dictionnaire du commerce équitable (2012)

Définit l'économie sociale et solidaire et positionne le commerce équitable par rapport à cette notion

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See detailImaginaires de la vie littéraire. Fiction, figuration, configuration
Dozo, Björn-Olav ULg; Glinoer, Anthony; Lacroix, Michel

Book published by Presses universitaires de Rennes (2012)

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See detailLa population, émergence d'un concept technologique
Oulc'hen, Hervé ULg

in Kiéfer, Audrey; Risse, David (Eds.) La biopolitique outre-Atlantique après Foucault (2012)

In his writings concerned with biopolitics Foucault gives the concept of population a central place and means to find its arising at the crossroads of several types of discourse : particularly about ... [more ▼]

In his writings concerned with biopolitics Foucault gives the concept of population a central place and means to find its arising at the crossroads of several types of discourse : particularly about species and the public. The genealogy of this bifocal concept is in keeping with some aspects of Canguilhem’s epistemological approach to the regulation of milieus, following his work on scientific ideology. Not only does such a parallel enable us to bring out the critical use of biopolitical concepts, it also leads us to examine the technological sequence which has made it possible for the concept of population to be determined. Such a sequence is articulated in three moments – utopia, practice, theory – which enable us to account for the evolution of the technology of police. [less ▲]

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See detailOrganisation du commerce équitable
Huybrechts, Benjamin ULg

in Blanchet, Vivien; Carimentrand, Aurélie (Eds.) Dictionnaire du commerce équitable (2012)

Définition et discussion de la notion d'organisation du commerce équitable

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See detailHow does elicitor and antimicrobial fengycin interact with plasma membranes of sensitive cells ?
Nasir, Mehmet Nail ULg; Eeman, Marc; Lins, Laurence ULg et al

Conference (2012, April)

Fengycin is characterized by its strong antifungal and low hemolytic activities. It has also been recently demonstrated that it has plant elicitor properties and is also able to enhance the elicitors’ ... [more ▼]

Fengycin is characterized by its strong antifungal and low hemolytic activities. It has also been recently demonstrated that it has plant elicitor properties and is also able to enhance the elicitors’ activity of surfactin. The cell target of its biological activities is supposed to be plasma membrane. In spite of these interesting biological activities, fengycin has not been extensively investigated probably because of the difficulties related to its production. In a first time; we have characterized the interfacial properties of fengycin by tensiometry measurements and demonstrated that this surface activity was pH-dependent. In a second time; we have investigated the interactions of the lipopeptide with membrane lipids using model membranes such as Langmuir monolayers and multilammelar vesicles (MLVs). Our results indicate that the lipopeptide was able to penetrate into different lipid monolayers showing a preference for sterol-containing monolayers. In order to better understand the mechanism of the interactions of fengycin with membranes at the molecular level, MLVs with or without fengycin have been analyzed by spectroscopic techniques. We have shown that conformational changes of the lipopeptide occurred in the presence of lipids and they were more significant in the presence of sterol. Moreover, tyrosine residues of the lipopeptide seem to play an important role in these interactions. In conclusion, we have determined that the surface-active behavior as well as the conformation of fengycin depends on its environment. We have also showed that the lipopeptide does not interact with all class of lipids in the same way and presents a preference for sterols. The presence of key groups within peptide cycle has also been supposed for the biological activities of the lipopeptide. [less ▲]

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See detailThe size and book-to-market effects revisited
Lambert, Marie ULg; Hübner, Georges ULg

E-print/Working paper (2012)

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See detailRéponses du Martin-pêcheur huppé Alcedo cristata à la perturbation de ses sites de nidification
Kisasa Kafutshi, Robert ULg

in Malimbus (2012), 34(28-38),

From 2004 to 2009, two colonies of Malachite Kingfisher were monitored by ringing the nestlings and adults, counting eggs and observing parental activity at the nests. Results show that 10 % of nests were ... [more ▼]

From 2004 to 2009, two colonies of Malachite Kingfisher were monitored by ringing the nestlings and adults, counting eggs and observing parental activity at the nests. Results show that 10 % of nests were destroyed by people in the Symphonies site as against 78 % in the Monastery site. Consequently, the number of nests available for breeding is lower at Monastery than at Symphonies. The number of fledged chicks per nest at Symphonies was twice that at the Monastery. Site fidelity is higher for the adults in the Symphonies site (26 %) than at the Monastery (11 %). This suggests that the Monastery site is less favourable to the survival of the Malachite Kingfisher than the Symphonies site. The best strategy to protect this species seems to be the maintenance of a good number of nesting sites. [less ▲]

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