Nuclear delivery of a therapeutic peptide by long circulating pH-sensitive liposomes: Benefits over classical vesicles.
Ducat, Emilie ; Deprez, Julie ; Gillet, Aline et al
in International Journal of Pharmaceutics (2011)
The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes ... [more ▼]
The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes [SPC:CHOL:PEG-750-DSPE (47:47:6 molar% ratio)] or pH-sensitive stealth liposomes [DOPE:CHEMS:CHOL:PEG(750)-DSPE (43:21:30:6 molar% ratio)] were used to deliver a therapeutic peptide to its nuclear site of action. The benefit of using stealth pH-sensitive liposomes was investigated and formulations were compared to classical liposomes in terms of size, shape, charge, encapsulation efficiency, stability and, most importantly, in terms of cellular uptake. Confocal microscopy and flow cytometry were used to evaluate the intracellular fate of liposomes themselves and of their hydrophilic encapsulated material. Cellular uptake of peptide-loaded liposomes was also investigated in three cell lines: Hs578t human epithelial cells from breast carcinoma, MDA-MB-231 human breast carcinoma cells and WI-26 human diploid lung fibroblast cells. The difference between formulations in terms of peptide delivery from the endosome to the cytoplasm and even to the nucleus was investigated as a function of time. Characterization studies showed that both formulations possess acceptable size, shape and encapsulation efficiency but cellular uptake studies showed the important benefit of the pH-sensitive formulation over the classical one, in spite of liposome PEGylation. Indeed, stealth pH-sensitive liposomes were able to deliver hydrophilic materials strongly to the cytoplasm. Most importantly, when encapsulated in pH-sensitive stealth liposomes, the peptide was able to reach the nucleus of tumorigenic and non tumorigenic breast cancer cells. [less ▲]Detailed reference viewed: 45 (33 ULg)
State of the Art of Existing Early Design Simulation Tools for Net Zero Energy Buildings: A Comparison of Ten Tools
Given the challenges to design Net Zero Energy Buildings (NZEBs) the use of Building Performance Simulation (BPS) tools during early design phases has been indispensable. In this context, BPS techniques ... [more ▼]
Given the challenges to design Net Zero Energy Buildings (NZEBs) the use of Building Performance Simulation (BPS) tools during early design phases has been indispensable. In this context, BPS techniques can be supportive when integrated early in the design process. However, architects suffer from BPS tools limitations during this decisive phase that addresses more the building geometry and envelope. To identify those limitations and as part of the International Energy Agency (IEA) Task 40: Towards Net Zero Energy Buildings, this report compares ten early design BPS tools. The aim is to define the potential of using and integrating the tools by architect during the design of NZEBs. The examined tools include HEED, e-Quest, ENERGY-10, Vasari, Solar Shoebox, Open Studio Plug-in, IES-VE- Ware, DesignBuilder, ECOTECT and BEopt. The comparison is based on five criteria including usability, optimization, interoperability, accuracy and design process integration of the tools. The results describe tools limitations and major requirements to meet the NZEBs objective implications. [less ▲]Detailed reference viewed: 37 (1 ULg)
First-principles design of efficient solar cells using two-dimensional arrays of core-shell and layered SiGe nanowires
; Malcioglu, Osman Baris ; Raty, Jean-Yves
in Physical Review. B, Condensed Matter and Materials Physics (2011), 83(3), 0353176-03531760353176
Research for third generation solar cell technology has been driven by the need to overcome the efficiency and cost problems encountered by current crystalline Si- and thin-film-based solar cells. Using ... [more ▼]
Research for third generation solar cell technology has been driven by the need to overcome the efficiency and cost problems encountered by current crystalline Si- and thin-film-based solar cells. Using first-principles methods, Ge/Si and Si/Ge core/shell and Si-Ge layered nanowires are shown to possess the required qualities for an efficient use in photovoltaic applications. We investigate the details of their band structure, effective mass, absorption property, and charge-carrier localization. The strong charge separation and improved absorption in the visible spectrum indicate a remarkable quantum efficiency that, combined with new designs, compares positively with bulk Si. [less ▲]Detailed reference viewed: 34 (10 ULg)
Recension de: Jean-François Courtine, La cause de la phénoménologie, Paris, P.U.F., Coll. « Épiméthée », 2007, 276 p.
E-print/Working paper (2011)Detailed reference viewed: 21 (1 ULg)
D'une fin de siècle l'autre. Passage furtif dans La Salle de bain.
in Textyles : Revue des Lettres Belges de Langue Française (2011)Detailed reference viewed: 16 (1 ULg)
Final results of a phase IIa, randomised, open-label trial to evaluate the percutaneous intramyocardial transplantation of autologous skeletal myoblasts in congestive heart failure patients: the SEISMIC trial.
; ; et al
in EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology (2011), 6(7), 805-12
AIMS: The SEISMIC study was an open-label, prospective, randomised study to assess the safety and feasibility of percutaneous myoblast implantation in heart failure patients with implanted cardioverter ... [more ▼]
AIMS: The SEISMIC study was an open-label, prospective, randomised study to assess the safety and feasibility of percutaneous myoblast implantation in heart failure patients with implanted cardioverter-defibrillators (ICD). METHODS AND RESULTS: Patients were randomised 2:1 to autologous skeletal myoblast therapy vs. optimal medical treatment. The primary safety end-point was defined as the incidence of procedural and device related serious adverse events, whereas the efficacy endpoints were defined as the change in global LVEF by MUGA scan, change in NYHA classification of heart failure and in the distance achieved during a six-minute walk test (6MW) at 6-month follow-up. Forty subjects were randomised to the treatment arm (n=26), or to the control arm (n=14). There were 12 sustained arrhythmic events and one death after episodes of ventricular tachycardia (VT) in the treatment group and 14 events in the control group (P=ns). At 6-month follow-up, 6MW distance improved by 60.3+/-54.1?meters in the treated group as compared to no improvement in the control group (0.4+/-185.7?meters; P=ns). In the control group, 28.6% experienced worsening of heart failure status (4/14), while 14.3% experienced an improvement in NYHA classification (2/14). In the myoblast-treatment arm, one patient experienced a deterioration in NYHA classification (8.0%), whereas five patients improved one or two classes (20.0%; P=0.06). However, therapy did not improve global LVEF measured by MUGA at 6-month follow-up. CONCLUSIONS: These data indicate that implantation of myoblasts in patients with HF is feasible, appears to be safe and may provide symptomatic relief, though no significant effect was detected on global LVEF. [less ▲]Detailed reference viewed: 9 (0 ULg)
L’ingegnoso impiego dei nomi dei santi: Guilhem de la Tor, De saint Martin me clam a saint Andreu
in Romance Philology (2011), 65Detailed reference viewed: 20 (2 ULg)
Bilan du monitoring des libellules dans les sites restaurés par le projet LIFE « Tourbières » sur le Plateau de Saint-Hubert.
Dufrêne, Marc ; ; et al
in Naturalistes Belges (Les) (2011), 92(3-4), 37-54Detailed reference viewed: 37 (3 ULg)
Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase.
; ; et al
in Nature (2011), 473(7346), 234-8
Notch signalling is a key intercellular communication mechanism that is essential for cell specification and tissue patterning, and which coordinates critical steps of blood vessel growth. Although subtle ... [more ▼]
Notch signalling is a key intercellular communication mechanism that is essential for cell specification and tissue patterning, and which coordinates critical steps of blood vessel growth. Although subtle alterations in Notch activity suffice to elicit profound differences in endothelial behaviour and blood vessel formation, little is known about the regulation and adaptation of endothelial Notch responses. Here we report that the NAD(+)-dependent deacetylase SIRT1 acts as an intrinsic negative modulator of Notch signalling in endothelial cells. We show that acetylation of the Notch1 intracellular domain (NICD) on conserved lysines controls the amplitude and duration of Notch responses by altering NICD protein turnover. SIRT1 associates with NICD and functions as a NICD deacetylase, which opposes the acetylation-induced NICD stabilization. Consequently, endothelial cells lacking SIRT1 activity are sensitized to Notch signalling, resulting in impaired growth, sprout elongation and enhanced Notch target gene expression in response to DLL4 stimulation, thereby promoting a non-sprouting, stalk-cell-like phenotype. In vivo, inactivation of Sirt1 in zebrafish and mice causes reduced vascular branching and density as a consequence of enhanced Notch signalling. Our findings identify reversible acetylation of the NICD as a molecular mechanism to adapt the dynamics of Notch signalling, and indicate that SIRT1 acts as rheostat to fine-tune endothelial Notch responses. [less ▲]Detailed reference viewed: 163 (18 ULg)
Evaluation over Greenland of WRF with GC-NET observations (1995-2005) by comparison with 2 other RCMs
Sacré, Bernard ; Fettweis, Xavier ; Doutreloup, Sébastien et al
In the context of climate change, the Greenland Ice Sheet (GrIS) plays an important role in sea level variation and oceanic thermohaline circulation changes. Unfortunately, Global Climate Models do not ... [more ▼]
In the context of climate change, the Greenland Ice Sheet (GrIS) plays an important role in sea level variation and oceanic thermohaline circulation changes. Unfortunately, Global Climate Models do not illustrate enough the characteristics of Greenland. To solve that, speciﬁc RCMs have been developed to take into account the features of polar regions. In this project, we compare three RCMs : the MAR model, the RACMO model and the Weather Research and Forecasting (WRF) model. WRF is an open source model developed by the Mesoscale and Microscale Meteorology Division of NCAR. We use here the standard WRF (version 3.2.1) and its polar optimization (called polar WRF). The MAR version tuned for the GrIS and coupled with a 1D surface scheme called SISVAT (for Soil Ice Snow Vegetation Atmosphere Transfer) is compared here. The version of RACMO is a speciﬁc version for the Greenland climate, RACMO2/GR. This model contains a special surface module for snow-ice treatment and other modiﬁcations concerning, for example, the surface turbulence heat ﬂux or the surface roughness. The comparison is made on a domain centered on Greenland at a 25-km horizontal resolution over the 1995-2005 period when Automatic Weather Station (AWS) measurements are available from the Greenland Climate NETwork (GC-NET). Statistics (mean, bias, RMSE, correlation coefﬁcient) are calculated for the near-surface temperature, surface pressure, 10m-wind speed and speciﬁc humidity for winter (October to April) and summer (May to September). In addition, the modeled snowfall are evaluated with ice core-based snow accumulation climatologies. Comparison shows a signiﬁcant improvement from RCMs compared to the reanalyses (NCEP2 and ERA-INTERIM) in respect to the AWS measurements. RACMO and MAR seem to compare better with observations than WRF. [less ▲]Detailed reference viewed: 58 (7 ULg)
Pour assurer la validité de l'interprétation de la mesure en éducation: aspects pratiques
Magis, David ; ;
in Raîche, Gilles; Paquette-Côté, Karine; Magis, David (Eds.) Des mécanismes pour assurer la validité de l'interprétation de la mesure en éducation. Tome 2: l'évaluation. (2011)Detailed reference viewed: 12 (6 ULg)
Des mécanismes pour assurer la validité de l’interprétation de la mesure en éducation. Tome 2: l'évaluation.
; ; Magis, David
Book published by Presses de l'Université du Québec (2011)Detailed reference viewed: 8 (2 ULg)
Novel Susceptibility Locus at 22q11 for Diabetic Nephropathy in Type 1 Diabetes
; ; et al
in PLoS ONE (2011), 9(11), 24053
Background: Diabetic nephropathy (DN) affects about 30% of patients with type 1 diabetes (T1D) and contributes to serious morbidity and mortality. So far only the 3q21–q25 region has repeatedly been ... [more ▼]
Background: Diabetic nephropathy (DN) affects about 30% of patients with type 1 diabetes (T1D) and contributes to serious morbidity and mortality. So far only the 3q21–q25 region has repeatedly been indicated as a susceptibility region for DN. The aim of this study was to search for new DN susceptibility loci in Finnish, Danish and French T1D families. Methods and Results: We performed a genome-wide linkage study using 384 microsatellite markers. A total of 175 T1D families were studied, of which 94 originated from Finland, 46 from Denmark and 35 from France. The whole sample set consisted of 556 individuals including 42 sib-pairs concordant and 84 sib-pairs discordant for DN. Two-point and multi-point non-parametric linkage analyses were performed using the Analyze package and the MERLIN software. A novel DN locus on 22q11 was identified in the joint analysis of the Finnish, Danish and French families by genome-wide multipoint nonparametric linkage analysis using the Kong and Cox linear model (NPLpairs LOD score 3.58). Nominal or suggestive evidence of linkage to this locus was also detected when the three populations were analyzed separately. Suggestive evidence of linkage was found to six additional loci in the Finnish and French sample sets. Conclusions: This study identified a novel DN locus at chromosome 22q11 with significant evidence of linkage to DN. Our results suggest that this locus may be of importance in European populations. In addition, this study supports previously indicated DN loci on 3q21–q25 and 19q13. [less ▲]Detailed reference viewed: 11 (1 ULg)
Action des cultures protectrices : cas des germes lactiques sur la flore alimentaire indésirable.
; Thonart, Philippe
in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 339-348Detailed reference viewed: 32 (5 ULg)
Tool use and Hafting in the Western European Middle Palaeolithic
in Toussaint, Michel; Di Modica, Kévin; Pirson, Stéphane (Eds.) Le Paléolithique Moyen en Belgique. Mélanges Marguerite Ulrix-Closset, Bulletin de la Société Royale Belge d’Etudes Géologiques et Archéologiques. Les Chercheurs de la Wallonie (2011)Detailed reference viewed: 77 (11 ULg)
Evaluation of the homing process in the treatment of atrophic nonunion fractures by percutaneous autologous osteoblast cell implantation.
; HAUZEUR, Jean-Philippe ; LECHANTEUR, Chantal et al
in Journal of Nuclear Medicine (The) (2011), 52(SUPPL), 510Detailed reference viewed: 9 (0 ULg)
RhoGDI alpha-dependent balance between RhoA and RhoC is a key regulator of cancer cell tumorigenesis
Ho, Thi Thanh Giang ; ; Dubail, Johanne et al
in Molecular Biology of the Cell (2011), 22(17), 3263-75
RhoGTPases are key signaling molecules regulating main cellular functions such as migration, proliferation, survival, and gene expression through interactions with various effectors. Within the RhoA ... [more ▼]
RhoGTPases are key signaling molecules regulating main cellular functions such as migration, proliferation, survival, and gene expression through interactions with various effectors. Within the RhoA-related subclass, RhoA and RhoC contribute to several steps of tumor growth, and the regulation of their expression affects cancer progression. Our aim is to investigate their respective contributions to the acquisition of an invasive phenotype by using models of reduced or forced expression. The silencing of RhoC, but not of RhoA, increased the expression of genes encoding tumor suppressors, such as nonsteroidal anti-inflammatory drug-activated gene 1 (NAG-1), and decreased migration and the anchorage-independent growth in vitro. In vivo, RhoC small interfering RNA (siRhoC) impaired tumor growth. Of interest, the simultaneous knockdown of RhoC and NAG-1 repressed most of the siRhoC-related effects, demonstrating the central role of NAG-1. In addition of being induced by RhoC silencing, NAG-1 was also largely up-regulated in cells overexpressing RhoA. The silencing of RhoGDP dissociation inhibitor alpha (RhoGDIalpha) and the overexpression of a RhoA mutant unable to bind RhoGDIalpha suggested that the effect of RhoC silencing is indirect and results from the up-regulation of the RhoA level through competition for RhoGDIalpha. This study demonstrates the dynamic balance inside the RhoGTPase network and illustrates its biological relevance in cancer progression. [less ▲]Detailed reference viewed: 39 (10 ULg)
Dirac-Fock calculations of forbidden transitions within 3pk and 3dk ground confogurations of highly charged tungsten ions (W47+ - W61+)
in Journal of Physics : B Atomic Molecular & Optical Physics (2011), 44
Adalimumab produces clinical remission and reduces extraintestinal manifestations in Crohn's disease: Results from CARE.
; Louis, Edouard ; et al
in Inflammatory Bowel Diseases (2011)
BACKGROUND: Data regarding the effectiveness of anti-tumor necrosis factor (TNF) agents for resolution of extraintestinal manifestations (EIMs) are scarce. The CARE study evaluated clinical effectiveness ... [more ▼]
BACKGROUND: Data regarding the effectiveness of anti-tumor necrosis factor (TNF) agents for resolution of extraintestinal manifestations (EIMs) are scarce. The CARE study evaluated clinical effectiveness, EIM resolution, and safety of adalimumab in a large pan-European cohort of patients with moderate to severe Crohn's disease (CD). METHODS: In all, 945 patients with a Harvey-Bradshaw Index (HBI) >/=7 enrolled in this multicenter, open-label phase IIIb trial. Patients received subcutaneous adalimumab, 160/80 mg at weeks 0/2, then 40 mg every other week. Dose adjustments were allowed for CD-related concomitant medications (from week 8) and adalimumab (from week 12). Clinical endpoints were analyzed through week 20 for all patients, and after stratification by prior infliximab exposure and by reason for discontinuing infliximab (primary nonresponse [PNR] or other). RESULTS: The remission rate (HBI <5) at week 20 was 52% (95% confidence interval, 49%-55%) overall, and was higher for infliximab-naive versus infliximab-exposed patients (62% versus 42%, P < 0.001). Remission rates were similar for PNR (37%) and other reasons (43%; P = 0.278). Of 497 patients with baseline EIMs, 51% were free of EIM signs and symptoms at week 20. Serious infectious adverse events were reported in 5% of patients. Opportunistic infections and malignancies were rare (</=1%). There was one case of demyelinating disease, but no occurrences of lupus, tuberculosis, or death. CONCLUSIONS: In this large cohort of patients, adalimumab treatment resulted in rates of clinical remission and EIM resolution exceeding 50%, and substantial rates of effectiveness in patients who had PNR to infliximab. Adalimumab was well tolerated, with safety consistent with prior reports. (Inflamm Bowel Dis 2011). [less ▲]Detailed reference viewed: 29 (3 ULg)
NZEB Definitions: current market status and certification in Belgium
; Attia, Shady
The recast of the European Performance of Buildings Directive (EPBD) requires the uptake of a definition of so called ‘nearly zero energy’ buildings (nZEB). Belgium was first to set a definition for a ... [more ▼]
The recast of the European Performance of Buildings Directive (EPBD) requires the uptake of a definition of so called ‘nearly zero energy’ buildings (nZEB). Belgium was first to set a definition for a ‘net zero energy house’ (NZEB) in 2009. Every year the definition is revised for consistency with shortcomings and emerging issues. The Belgian definition raised many questions regarding (1) the net balance, (2) the application and calculation method (3) the indoor air quality and comfort, (4) energy use included in the balance, (5) the type of buildings, (6) the accepted renewable energy supply options, (7) the quality assurance and monitoring and (8) energy storage and the connection to the energy infrastructure. Therefore, the paper presents a review on the current Belgian definition and its market status in comparison with the international context. The aim of the paper is to review current definition discussions and pave the way to a more consistent definition. [less ▲]Detailed reference viewed: 9 (1 ULg)