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See detailFunctional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas reinhardtii
Lecler, Renaud ULg; Godaux, Damien ULg; Vigeolas, Hélène ULg et al

in International Journal of Hydrogen Energy (2011), 36

In this paper, mitochondrial mutants of Chlamydomonas reinhardtii defective for respiratory complex I (NADH:ubiquinone oxidoreductase), complex III (ubiquinol cytochrome c oxidoreductase) and both ... [more ▼]

In this paper, mitochondrial mutants of Chlamydomonas reinhardtii defective for respiratory complex I (NADH:ubiquinone oxidoreductase), complex III (ubiquinol cytochrome c oxidoreductase) and both complexes I and III were analyzed for H2 photoproduction. Several parameters were followed during the S-deficiency stage and the anaerobic stage leading to H2 photoproduction. At the early aerobic S-deficiency stage, starch and neutral lipids accumulated in all strains but their amount was significantly decreased in mutants compared to wild type. During the H2 photoproduction process, whereas starch content strongly decreased in all strains, neutral lipid amount remained nearly unchanged, suggesting that starch degraded by glycolysis is the preferential substrate for energy production during anaerobiosis. The mutants displayed a decrease in H2 photoproduction correlating to the number of active mitochondrial proton-pumping sites lost in the strains. Our results thus highlight the critical role of oxidative phosphorylation during the first (aerobic) stage of S-starvation when carbon resources are accumulated. [less ▲]

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See detailA finite element analysis of the bending and the bendability of metallic sheets
Ben Bettaieb, Mohamed ULg; Lemoine, Xavier; Bouaziz, O. et al

in International Journal of Material Forming (2011), 4(3), 283-297

The main objective of this paper is to study the bendability of metallic sheets by using the finite element method. In this aim, two variants of an advanced Gurson-Tvergaard-Needleman model [1, 2] are ... [more ▼]

The main objective of this paper is to study the bendability of metallic sheets by using the finite element method. In this aim, two variants of an advanced Gurson-Tvergaard-Needleman model [1, 2] are implemented in the home made FE code LAGAMINE [3, 4] and coupled with the Thomason model to predict the coalescence of voids. This advanced model is an extension of the original one to take into account of the plastic anisotropy and the mixed (isotropic + kinematic) hardening of the matrix. The difference between the two variants is related to the modeling of the damage evolution. As the advanced model is used to study the bending process, its yield function is slightly modified in order to take into account the loadings with negative triaxiality ratios. These present implementations are used to simulate the pure bending process and to predict the bendability of dual phase (DP) steel. The combined effect of an initial geometrical imperfection and damage evolution on the bendability is also studied. [less ▲]

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See detailNucleolar structure across evolution: the transition between bi- and tri-compartmentalized nucleoli lies within the class Reptilia.
Lamaye, Francoise; Galliot, Sonia; Alibardi, Lorenzo et al

in Journal of Structural Biology (2011), 174(2), 352-9

Two types of nucleolus can be distinguished among eukaryotic cells: a tri-compartmentalized nucleolus in amniotes and a bi-compartmentalized nucleolus in all the others. However, though the nucleolus ... [more ▼]

Two types of nucleolus can be distinguished among eukaryotic cells: a tri-compartmentalized nucleolus in amniotes and a bi-compartmentalized nucleolus in all the others. However, though the nucleolus' ultrastructure is well characterized in mammals and birds, it has been so far much less studied in reptiles. In this work, we examined the ultrastructural organization of the nucleolus in various tissues from different reptilian species (three turtles, three lizards, two crocodiles, and three snakes). Using cytochemical and immunocytological methods, we showed that in reptiles both types of nucleolus are present: a bi-compartmentalized nucleolus in turtles and a tri-compartmentalized nucleolus in the other species examined in this study. Furthermore, in a given species, the same type of nucleolus is present in all the tissues, however, the importance and the repartition of those nucleolar components could vary from one tissue to another. We also reveal that, contrary to the mammalian nucleolus, the reptilian fibrillar centers contain small clumps of condensed chromatin and that their surrounding dense fibrillar component is thicker. Finally, we also report that Cajal bodies are detected in reptiles. Altogether, we believe that these results have profound evolutionarily implications since they indicate that the point of transition between bipartite and tripartite nucleoli lies at the emergence of the amniotes within the class Reptilia. [less ▲]

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See detailMid infrared and fluorescence spectroscopies coupled with factorial discriminant analysis technique to identify sheep milk from different feeding systems
Karoui, Romdhane; Hammami, Moncef; Rouissi, Hamadi et al

in Food Chemistry (2011), 127(2), 743-748

Mid infrared spectroscopy (MIR) combined with multivariate data analysis was used to discriminate between ewes milk samples according to their feeding systems (controls, ewes fed scotch bean and ewes fed ... [more ▼]

Mid infrared spectroscopy (MIR) combined with multivariate data analysis was used to discriminate between ewes milk samples according to their feeding systems (controls, ewes fed scotch bean and ewes fed soybean). The MIR spectra were scanned throughout the first 11 weeks of the lactation stage. When factorial discriminant analysis (FDA) with leave one-out cross-validation was applied, separately, to the three spectral regions in the MIR (i.e. 3000-2800, 1700-1500 and 1500-900 cm(-1)), the classification rate was not satisfactory. Therefore, the first principal component (PCs) scores (corresponding to 3, 10 and 10 for, respectively, the 3000-2800, 1700-1500 and 1500-900 cm(-1)) of the principal component analysis (PCA) extracted from each of the data sets were pooled (concatenated) into a single matrix and analysed by FDA. Correct classification amounting to 71.7% was obtained. Finally, the same procedure was applied to the MIR and fluorescence data sets and 98% of milk samples were found to be correctly classified. Milk samples belonging to control and soybean groups were 100% correctly classified. Regarding milk samples originating from the scotch bean group, only 2 out of 33 samples were misclassified. It was concluded that concatenation of the data sets collected from the two spectroscopic techniques is an efficient tool for authenticating milk samples according to their feeding systems, regardless of the lactation stage. (C) 2011 Published by Elsevier Ltd. [less ▲]

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See detailAttempt to rescue discarded human liver grafts by end ischemic hypothermic oxygenated machine perfusion.
Vekemans, K.; van Pelt, J.; Komuta, M. et al

in Transplantation Proceedings (2011), 43(9), 3455-9

In a porcine liver transplant model, a brief period of oxygenated hypothermic machine perfusion (HMP) at the end of simple cold storage (SCS) has been shown to improve the viability of damaged liver ... [more ▼]

In a porcine liver transplant model, a brief period of oxygenated hypothermic machine perfusion (HMP) at the end of simple cold storage (SCS) has been shown to improve the viability of damaged liver grafts. To test the clinical validity of this strategy, we randomized SCS-discarded human liver grafts to either 4 hours of HMP (n = 13) or an additional 4 hours of SCS (n = 14). All livers were then warm reperfused to mimic ischemia-reperfusion injury ex vivo. The settings for HMP were: portal vein: 3 mm Hg, 300 mL/min and hepatic artery: 20 mm Hg, po(2): 300 mm Hg. Perfusion used Kidney Machine Perfusion Solution at 4 degrees C to 8 degrees C. During warm reperfusion, aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) values were higher (P < .015) among the SCS versus HMP methods at all times. The AST slope was lower in HMP versus SCS (P = .01). The LDH slope tended to be lower for HMP versus SCS (P = .07). Morphological scores were not different between HMP and SCS. At the start of warm reperfusion, MAPK was lower in HMP versus SCS (P = .02). Endothelin-1 (EDN1) and ICAM-1 tended to be lower in HMP versus SCS (P = .1 and .07, respectively). No difference was noted in MAPK, EDN1, and ICAM-1 after 60 or 120 minutes of warm reperfusion. In conclusion, HMP down-regulated MAPK and tended to reduce EDN1 and ICAM-1 mRNA in human liver grafts. During warm reperfusion, HMP versus SCS livers showed reduced AST and LDH release but no morphological difference. Further optimization of liver HMP may require different timing/duration of perfusion and/or an higher perfusion temperature. [less ▲]

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See detailpi N transition distribution amplitudes: Their symmetries and constraints from chiral dynamics
Pire, Bernard; Semenov-Tyan-Shanskiy, Kirill ULg; Szymanowski, Lech

in Physical Review. D : Particles and Fields (2011), 84

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See detailApport au patrimoine commun et avance sur part de communauté: deux modalités originales. Note sous Liège, 7 septembre 2010
Rousseau, Laura ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2011)

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See detailVariable Neighborhood Search applied to the Vehicle Routing Problem
François, Véronique ULg

Learning material (2011)

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See detailIndependent single photon emitters – a versatile source for the generation
von Zanthier, J.; Maser, A.; Wiegner, R. et al

Conference (2011)

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See detailKurt Smith, Matter Matters. Metaphysics and Methodology in the Early Modern Period
Dubouclez, Olivier ULg

in Bulletin cartésien (2011)

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See detailGobernabilidad desde las agrupaciones usuarias del agua con fines agrícolas en 8 municipios del territorio de la Mancomunidad de Municipios de la cuenca del río Naranjo (MANCUERNA)
Estrada Orozco, Nick Kenner ULg; Mux, Victor

in Tikalia (2011), XXIX(1-2), 37-46

En Guatemala, para alcanzar los Objetivos de desarrollo del Milenio se implemen- tan Programas Conjuntos con el apoyo del Sistema de Naciones Unidas. Existen en la actualidad cinco Programas Conjuntos ... [more ▼]

En Guatemala, para alcanzar los Objetivos de desarrollo del Milenio se implemen- tan Programas Conjuntos con el apoyo del Sistema de Naciones Unidas. Existen en la actualidad cinco Programas Conjuntos apoyando temas de seguridad alimen- taria, construcción de la paz, cambio climático, género y gobernabilidad económi- ca del agua. En el marco del programa conjunto: “Gobernabilidad económica en agua y sanea- miento” se decidió realizar un estudio que permitiera conocer la gobernabilidad en el sector a partir de hacer un inventario de las agrupaciones usuarias de agua con fines agrícolas y conocer algunas de sus características, este estudio contó con la asesoría técnica de la Organización de las Naciones Unidas para la Agricultura y la Alimentación –FAO– y el apoyo en campo de las comunidades asociadas por el agua y el medio ambiente de la cuenca del río Naranjo –CADISNA– en coordina- ción con el Ministerio de Agricultura Ganadería y Alimentación –MAGA– y go- biernos locales. El estudio se realizó en 8 municipios, 3 del departamento de Quetzaltenango y 5 de San Marcos, se identificaron 126 grupos de riego, de ellos, 73 pudieron ser carac- terizados. Se conoció que el 92% de los 73 grupos tiene una junta directiva que vela por el funcionamiento del sistema y la producción se ve diversificada en al menos 14 diferentes hortalizas siendo la principal el cultivo de la Papa. Sin embar- go sólo el 45% paga una tarifa a su grupo y el 60% de los usuarios necesita crédito para poder producir, haciendo uso de al menos 271.99 Lts/seg de agua para fines agrícolas. [less ▲]

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See detailInvolvement of z-MMP-2 in Zebrafish lymphangiogenesis
Paupert, Jenny ULg; Pendeville, Hélène; Detry, Benoît ULg et al

Conference (2011)

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See detailQuantifying hyporheic exchange at high spatial resolution using natural temperature variations along a first order stream
Westhoff, Martijn ULg; Gooseff, M. N.; Bogaard, T. A. et al

in Water Resources Research (2011), 47

Hyporheic exchange is an important process that underpins stream ecosystem function, and there have been numerous ways to characterize and quantify exchange flow rates and hyporheic zone size. The most ... [more ▼]

Hyporheic exchange is an important process that underpins stream ecosystem function, and there have been numerous ways to characterize and quantify exchange flow rates and hyporheic zone size. The most common approach, using conservative stream tracer experiments and 1-D solute transport modeling, results in oversimplified representations of the system. Here we present a new approach to quantify hyporheic exchange and the size of the hyporheic zone (HZ) using high-resolution temperature measurements and a coupled 1-D transient storage and energy balance model to simulate in-stream water temperatures. Distributed temperature sensing was used to observe in-stream water temperatures with a spatial and temporal resolution of 2 and 3 min, respectively. The hyporheic exchange coefficient (which describes the rate of exchange) and the volume of the HZ were determined to range between 0 and 2.7 × 10−3 s−1 and 0 and 0.032 m3 m−1, respectively, at a spatial resolution of 1–10 m, by simulating a time series of in-stream water temperatures along a 565 m long stretch of a small first-order stream in central Luxembourg. As opposed to conventional stream tracer tests, two advantages of this approach are that exchange parameters can be determined for any stream segment over which data have been collected and that the depth of the HZ can be estimated as well. Although the presented method was tested on a small stream, it has potential for any stream where rapid (in regard to time) temperature change of a few degrees can be obtained. [less ▲]

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See detailMultiparametric observation and analysis of the Sea
Alvera Azcarate, Aïda ULg; Poulain, Pierre-Marie

in Ocean Dynamics (2011)

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See detailAtlas linguistique de la Wallonie. Tome 17. Famille, vie et relations sociales
Baiwir, Esther ULg

Book published by PULg - Presses universitaires de Liège - Sciences Humaines (2011)

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See detailTreatment of postmenopausal women with osteoporosis for 5 years with denosumab : two-year results from the FREEDOM trial extension
Chapurlat, R.; Bone, H. G.; Brandi M L et al

in Annals of the Rheumatic Diseases (2011), 70(S3), 166-167

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See detailLaurent (Brice), Les politiques des nanotechnologies. Pour un traitement démocratique d’une science émergente
Thoreau, François ULg

in Politix : Revue des Sciences Sociales du Politique (2011), 2011/4(96), 187-190

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See detailCellular uptake of liposomes monitored by confocal microscopy and flow cytometry
Ducat, Emilie ULg; Evrard, Brigitte ULg; Peulen, Olivier ULg et al

in Journal of Drug Delivery Science and Technology [=JDDST] (2011), 21(6), 469-477

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See detailLe secteur immobilier face au développement du droit européen des contrats
Kohl, Benoît ULg; Mallet-Bricout, B.; Périnet-Marquet, H. et al

in Behar-Touchais, M.; Chagny, M. (Eds.) Livre vert sur le droit européen des contrats. Réponses du réseau Trans Europe Expert (2011)

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