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See detailFirst retrievals of carbon tetrafluoride (CF4) from ground-based FTIR measurements: production and analysis of the two-decadal time series above the Jungfraujoch
Duchatelet, Pierre ULg; Zander, Rodolphe ULg; Mahieu, Emmanuel ULg et al

in Geophysical Research Abstracts (2011)

Carbon tetrafluoride (CF4 or PFC-14) is a potent greenhouse gas that is almost 7400 times more effective (100-yr horizon) than CO2 on a per molecule basis (IPCC, 2007). This high global warming potential ... [more ▼]

Carbon tetrafluoride (CF4 or PFC-14) is a potent greenhouse gas that is almost 7400 times more effective (100-yr horizon) than CO2 on a per molecule basis (IPCC, 2007). This high global warming potential, coming from its medium absorbance combined with a very long atmospheric lifetime (>50000 years; Ravishankara et al., 1993), makes CF4 a key species among the various greenhouse gases targeted by the Kyoto Protocol. In the Northern hemisphere, current atmospheric CF4 concentrations are close to 78 pptv, with a large fraction (around 35 pptv, Mühle et al., 2010) coming from natural processes like lithospheric emissions (Harnisch and Eisenhauer, 1998). In addition, CF4 has been used increasingly since the eighties in electronic and semiconductors industry. The primary aluminum production processes have also been clearly identified as an important anthropogenic source of CF4 emissions. The partitioning between these two main sources is however problematic, principally due to lacking or incomplete CF4 emission factors from inventories performed in industrial fields (e.g. International Aluminum Institute, 2009). Recent in situ ground level measurements of CF4 in the Northern hemisphere (Khalil et al., 2003; Mühle et al., 2010) or remotely from space (Rinsland et al., 2006) have indicated a significant slowdown in the increase rate of atmospheric CF4. This probably results from measures adopted by the aluminum industry aiming at the reduction of the frequency and duration of “anode effects” and therefore of related PFCs emissions (International Aluminum Institute, 2009). The present contribution reports on the long-term evolution (1990-2010) of the atmospheric carbon tetrafluoride total vertical abundance derived from ground-based Fourier transform infrared (FTIR) solar spectroscopy observations around 1285 cm-1 at the Jungfraujoch (46.5°N, 8.0°E, 3580m asl) and compares our findings with results available in the literature. To our knowledge, no equivalent time series (i.e. based on ground-based FTIR technique) has been published to date. [less ▲]

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See detailNotched Specimens Fracture Prediction with an Advanced GTN Model
Fansi Tchonko, Joseph ULg; Ben Bettaieb, Mohamed ULg; Balan, Tudor et al

in Key Engineering Materials [=KEM] (2011), 488-489

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See detailLe Michelsberg de Pont-de-Bonne (province de Liège, Belgique)
Delye, Emmanuel ULg; Gilson, Simon-Pierre; Noiret, Pierre ULg

in Revue Archéologique de Picardie (2011)

Since the beginning of research on the éperon barré of Pont-de-Bonne (Modave, Belgium), we have recovered artefacts attributed to the Middle Neolithic. More recently, during excavation of the entrance ... [more ▼]

Since the beginning of research on the éperon barré of Pont-de-Bonne (Modave, Belgium), we have recovered artefacts attributed to the Middle Neolithic. More recently, during excavation of the entrance system of the fortification, a pot hole and a waste accumulation zone have been analysed. These structures contained abundant archaeological material (ceramic sherd, lithic and bone artefact and fauna). Three radiometric dates have been obtained. The ceramic assemblage is typical of the Belgian Michelsberg and a decorated vase shows Bischheim affinities. [less ▲]

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See detailTechniques de séchage des starters lactiques et mécanismes affectant la viabilité cellulaire suite à la lyophilisation
Coulibaly, Ibourahema ULg; Dubois Dauphin, Robin ULg; Danthine, Sabine ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 287-299

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See detailQuantitative food webs of herbivore and related beneficial community in non–crop and crop habitats
Alhmedi, Ammar; Haubruge, Eric ULg; D’Hoedt, Sandrine et al

in Biological Control (2011), 58

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See detailSimulation von Extraktionskolonnen in der industriellen Praxis
Buchbender, Florian; Schmidt, Markus; Pfennig, Andreas ULg et al

in Chemie Ingenieur Technik (2011), 84(4), 540-546

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See detailBrain functional integration decreases during propofol-induced loss of consciousness.
Schrouff, Jessica ULg; Perlbarg, Vincent; Boly, Mélanie ULg et al

in NeuroImage (2011), 57(1), 198-205

Consciousness has been related to the amount of integrated information that the brain is able to generate. In this paper, we tested the hypothesis that the loss of consciousness caused by propofol ... [more ▼]

Consciousness has been related to the amount of integrated information that the brain is able to generate. In this paper, we tested the hypothesis that the loss of consciousness caused by propofol anesthesia is associated with a significant reduction in the capacity of the brain to integrate information. To assess the functional structure of the whole brain, functional integration and partial correlations were computed from fMRI data acquired from 18 healthy volunteers during resting wakefulness and propofol-induced deep sedation. Total integration was significantly reduced from wakefulness to deep sedation in the whole brain as well as within and between its constituent networks (or systems). Integration was systematically reduced within each system (i.e., brain or networks), as well as between networks. However, the ventral attentional network maintained interactions with most other networks during deep sedation. Partial correlations further suggested that functional connectivity was particularly affected between parietal areas and frontal or temporal regions during deep sedation. Our findings suggest that the breakdown in brain integration is the neural correlate of the loss of consciousness induced by propofol. They stress the important role played by parietal and frontal areas in the generation of consciousness. [less ▲]

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See detailMicrobial Diversity and Processes in Lake Kivu (East Africa)
Llirós, M.; Darchambeau, François ULg; Garcia-Armisen, T. et al

Conference (2011)

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3 ... [more ▼]

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3, respectively) in the deep anoxic monimolimnion (from 60 o 480 m depth). Although there are a wealth of information on the ecology of small eukaryotes and their trophic role on Kivu, available information on prokaryotic planktonic assemblages is scarce. Molecular analysis of archaeal and bacterial communities showed a vertical segregation imposed by the permanent redoxcline. In relation to Bacteria, Actinobacteria, Betaproteobacteria, Green Sulfur Bacteria and Bacteroidetes were the most commonly retrieved groups. For Archaea, a marked dominance of Thaumarchaeota and Crenarchaeota (75% of all archaeal OTUs) over Euryarchaeota was observed. In the anoxic hypolimnion, Euryarchaoeta (Methanosarcinales and Methanocellales) lineages together with Miscellaneous Crenarchaeotic Group phylotypes were mainly recovered. In turn, Thaumarchaeota phylotypes were recovered in oxic and suboxic waters. CARDFISH analyses over the first 100 m revealed the dominance of Bacteria (51.4% – 95.7% of DAPI-stained cells), especially Actinobacteria (epilimnion), Betaproteobacteria (oxic-anoxic interface) and Bacteroidetes (upper hypolimnion), over Archaea (1.0% – 4.5%; maximum abundances at the oxic-anoxic interface). In turn, flow cytometry evidenced the dominance of HNA cells in the euphotic layer, whereas the proportion of LNA cells increased with depth. HNA and LNA populations were still observed in the anoxic hypolimnion suggesting facultative or strict anaerobic metabolisms. The detection of distinct depth maxima of nitrate, nitrite, archaeal amoA and Marine Thaumarchaeota 16S gene copy numbers together with regularly detection of deep maxima of 3H-Thymidine uptake, and the presence of low-light adapted GSB species point towards a strong link of N, C, and S cycles in the redoxcline of Lake Kivu. [less ▲]

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See detailCharacterization of the response of GFP microbial biosensors sensitive to substrate limitation in scale-down bioreactors
Delvigne, Frank ULg; Brognaux, Alison ULg; Gorret, Nathalie et al

in Biochemical Engineering Journal (2011), 55(2), 131-139

The dynamics of microbial stress response in intensive cultivation conditions remains not completely understood. In this work, two green fluorescent protein (GFP) transcriptional reporters have been used ... [more ▼]

The dynamics of microbial stress response in intensive cultivation conditions remains not completely understood. In this work, two green fluorescent protein (GFP) transcriptional reporters have been used as biosensors of the heterogeneities generated in a two-compartment scale-down reactor. The stress promoters have been chosen for their responsiveness to carbon limitation corresponding to the global substrate profiles encountered in intensive fed-batch cultures. From our results, it can be concluded that the exposure of microbial cells to substrates heterogeneities tends to decrease the GFP expression level in fed-batch mode. Fluorescence intensities have been monitored at the single cell level by using flow cytometry. During the course of the fed-batch culture, a drop at the level of the intracellular GFP content has been observed for the two scale-down operating conditions and for the two promoters sensitive to substrate limitation (rpoS and csiE). The fluorescence drop can be attributed to the repression of these promoters but also to the release of GFP to the extracellular medium according to the increase of the fluorescence level of the supernatant. This leakage has been observed for all the operating conditions, i.e. the scale-down reactors and the culture operating in the normal mode, i.e. in a well-mixed bioreactor. Interestingly, GFP leakage is more pronounced in the case of the cultures operated in the normal mode. Indeed, staining by propidium iodide tends to be more elevated for the microbial cells cultured under the normal mode by comparison with those cultured in scale-down conditions, indicating a higher permeability of the membrane. These results suggest that GFP microbial biosensors could be used to detect simultaneously mixing imperfections and their impact on the viability of microorganisms. [less ▲]

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See detailFrom shallow water to deep mound - sedimentology and stromatoporoids paleoecology from the Frasnian (Upper Devonian) of Belgium
Da Silva, Anne-Christine ULg; Kershaw, Stephen; Boulvain, Frédéric ULg

in Kölner Forum für Geologie und Paläontologie (2011), 19

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See detailDiscovery of English Grain Aphid (Hemiptera: Aphididae) Biotypes in China
Xu, Zhao-Huan; Chen, Julian; Cheng, Deng-Fa et al

in Journal of Economic Entomology (2011), 104(3), 1080-1086

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See detailMolecular recognition force spectroscopy
Willet, Nicolas ULg; Lamprecht, Constanze; Rankl, Christian et al

in Duwez, Anne-Sophie; Willet, Nicolas (Eds.) Molecular manipulation with atomic force microscopy (2011)

This chapter describes the state of the art in molecular recognition force spectroscopy performed by AFM. The different aspects of the topic are discussed, as the appropriate techniques for the ... [more ▼]

This chapter describes the state of the art in molecular recognition force spectroscopy performed by AFM. The different aspects of the topic are discussed, as the appropriate techniques for the functionalization of cantilever tips and for the preparation of (biological) samples. The principles of single-molecule force spectroscopy are then explained, together with exciting and recent examples on synthetic and biological samples. Finally, the main techniques to map molecular recognition interactions are reviewed and discussed in terms of performances. Novel and interesting applications illustrate the use of these imaging methods. [less ▲]

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See detailThe future soil capacity of Podzols to stabilize soil organic carbon through formation of imogolite
Cornelis, Jean-Thomas ULg; Caignet, Isabelle; Delvaux, Bruno

Scientific conference (2011)

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See detailInfluence of clay in limestone fillers for self compacting cement based composites
Courard, Luc ULg; Michel, Frédéric ULg; Piérard, Julie

in Construction & Building Materials (2011), 25

High workability together with a good resistance to segregation is needed for cement based composites, specifically fresh Self-Compacting Mortars (SCM); in this prospect, the amount of coarse materials ... [more ▼]

High workability together with a good resistance to segregation is needed for cement based composites, specifically fresh Self-Compacting Mortars (SCM); in this prospect, the amount of coarse materials has to be reduced and replaced by fine material. Several limestone fillers are here compared and analysed. These by-products are issued from different industrial sectors, such as the aggregate and lime production industry (quarrying operations) and the ornamental stones industry (sawing operations). Particular attention has been paid to clay content, as consistency of fresh mortars was varying, while other physical characteristics like granulometry remained the same. Relationship between the physico-chemical properties of the fillers and the properties of fresh and hardened mortars are brought forward. Even if it may affect fresh properties of mortars, results clearly show that clay type and content has minor influence than limestone filler itself. [less ▲]

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See detailImpact of chronic graft-versus-host disease (GVHD) after reduced intensity conditioning (RIC) allogeneic stem cell transplantation (allo-SCT) as treatment for acute myeloid leukemia (AML) : a survey from the Acute Leukemia Working Party of the EBMT
Baron, Frédéric ULg; Labopin, Myriam; Niederwieser, Dietger et al

in Blood (2011), 118

The goal of RIC allo-SCT is to harness the graft-versus-leukemia (GVL) effect, while minimizing toxicities and the risk of GVHD. However, prior studies have shown a lower risk of relapse in AML patients ... [more ▼]

The goal of RIC allo-SCT is to harness the graft-versus-leukemia (GVL) effect, while minimizing toxicities and the risk of GVHD. However, prior studies have shown a lower risk of relapse in AML patients (pts) who experienced chronic GVHD after RIC allo-SCT versus in those who did not. Here, we investigated the impact of occurrence of GVHD on transplantation outcomes in a large cohort of AML pts given allogeneic PBSC after RIC conditioning. Data from 1859 AML pts in first (n=1439) or second (n=420) CR transplanted between 2000 and 2009 following a RIC regimen at EBMT affiliated centres were analyzed. Pts were given PBSC from HLA-identical sibling (MRD, n=1208), or from HLA-matched unrelated donors (MUD, n=651). Median pt age at transplantation was 56 y (range, 18–77). 338 male pts were given grafts from female donors. RIC was based on low-dose TBI in 520 (28%) pts, while the remaining pts received chemotherapy-based RIC. ATG was given in 269 (22%) MRD and in 267 (41%) MUD recipients, respectively, while 151 (13%) MRD and 165 (25%) MUD recipients received in-vivo T cell depletion with alemtuzumab. The impact of chronic GVHD on relapse risk, non-relapse mortality (NRM), leukemia-free survival (LFS), and overall survival (OS) was assessed using time-dependent multivariate Cox models and in a landmark analysis at 18 months after transplant. Three-year incidences of relapse, NRM, LFS and OS were 34±1%, 15±1%, 51±2% and 60±2% in MRD recipients, respectively, and 34±2% (p=NS), 24±2% (P<0.001), 42±2% (P=0.001) and 47±2% (P=0.001) in MUD recipients, respectively. Grade II, III and IV acute GVHD were observed in 133 (11%), 61 (5%) and 30 (2%) MRD recipients and in 119 (18%), 41 (6%) and 24 (4%) MUD recipients, respectively. The 3-y cumulative incidence of chronic GVHD was 47%. Fifty-three percent of patients with chronic GVHD had extensive chronic GVHD, while the remaining 47% had limited chronic GVHD. In multivariate analyses, occurrence of grade II-IV acute GVHD was associated with a lower risk of relapse (HR=0.8; P=0.04), a higher risk of chronic (HR=2.2; P<0.001) and extensive chronic GVHD (HR=2.8; P<0.001), a higher risk of NRM (HR=2.4 P<0.001), a worsened LFS (HR=1.3; P=0.01), and a worsened OS (HR=1.5; P<0.001). In multivariate time-dependent analyses, occurrence of limited chronic GVHD was associated with a lower risk of relapse (HR=0.7; P=0.05), comparable NRM (HR=1.4; P=0.16), comparable LFS (HR=0.9; P=0.29) and better OS (HR=0.5; P<0.001), while occurrence of extensive chronic GVHD was associated with a lower risk of relapse (HR=0.6; P=0.01), higher NRM (HR=3.2; P<0.001), a trend for worsened LFS (HR=1.3; P=0.06) and comparable OS (HR=0.9; P=0.34). The median interval from transplantation to occurrence of chronic GVHD was 163 (range, 100–1545) days. To further assess the graft-versus-leukemia effect of chronic GVHD, we performed a landmark analysis in patients who were leukemia-free at 18 months after transplantation (n=776). Median follow-up from this landmark time-point was 24 (range, 0.1–112) months. Two-year relapse, NRM, LFS and OS were 16±2%, 2.5±1%, 82±2%, and 89±2%, respectively, in patients without chronic GVHD before the landmark time-point, versus 9±1% (P=0.001), 8±1% (P<0.001), 83±2% (P=0.65), and 86±2% (P=0.38), respectively, in patients with chronic GVHD before the landmark time-point.In conclusion, in this cohort of AML patients transplanted in remission, occurrence of chronic GVHD was associated with a lower risk of relapse that translated to better OS in patients with limited chronic GVHD but not in those with extensive chronic GVHD who experienced higher long term NRM, highlighting the need for long term prospective assessment of long term effects and quality of life in patients receiving RIC allo-SCT. [less ▲]

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See detailLa tradizione fiorentina della Supplicatione d’Italia al re di Francia. Con una nuova edizione critica
Moreno, Paola ULg

in Filologia e Critica (2011), XXXV(2), 1-33

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