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See detailExploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements
Berlemont, Renaud ULg; Pipers; Delsaute, Maud ULg et al

in Revista Argentina de Microbiologia (2011)

Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications ... [more ▼]

Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 14 amylase-, 3 protease-, and 11 cellulase-producing clones were detected by activity-driven screening, with apparent maximum activities around 35 °C for both amylolytic and lipolytic enzymes, and 35-55 °C for cellulases, as observed for other cold-adapted enzymes. However, the behavior of at least one of the studied cellulases is more compatible to that observed for mesophilic enzymes. These enzymes are usually still active at temperatures above 60 °C, probably resulting in a psychrotolerant behavior in Antarctic soils. Metagenomics allows to access novel genes encoding for enzymatic and biophysic properties from almost every environment with potential benefits for biotechnological and industrial applications. Only intI- and tnp-like genes were detected by PC R, encoding for proteins with 58-86%, and 58-73% amino acid identity with known entries, respectively. Two clones, BAC 27A-9 and BAC 14A-5, seem to present unique syntenic organizations, suggesting the occurrence of gene rearrangements that were probably due to evolutionary divergences within the genus or facilitated by the association with transposable elements. The evidence for genetic elements related to recruitment and mobilization of genes (transposons/integrons) in an extreme environment like Antarctica reinforces the hypothesis [less ▲]

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See detailMicrobial Diversity and Processes in Lake Kivu (East Africa)
Llirós, M.; Darchambeau, François ULg; Garcia-Armisen, T. et al

Conference (2011)

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3 ... [more ▼]

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3, respectively) in the deep anoxic monimolimnion (from 60 o 480 m depth). Although there are a wealth of information on the ecology of small eukaryotes and their trophic role on Kivu, available information on prokaryotic planktonic assemblages is scarce. Molecular analysis of archaeal and bacterial communities showed a vertical segregation imposed by the permanent redoxcline. In relation to Bacteria, Actinobacteria, Betaproteobacteria, Green Sulfur Bacteria and Bacteroidetes were the most commonly retrieved groups. For Archaea, a marked dominance of Thaumarchaeota and Crenarchaeota (75% of all archaeal OTUs) over Euryarchaeota was observed. In the anoxic hypolimnion, Euryarchaoeta (Methanosarcinales and Methanocellales) lineages together with Miscellaneous Crenarchaeotic Group phylotypes were mainly recovered. In turn, Thaumarchaeota phylotypes were recovered in oxic and suboxic waters. CARDFISH analyses over the first 100 m revealed the dominance of Bacteria (51.4% – 95.7% of DAPI-stained cells), especially Actinobacteria (epilimnion), Betaproteobacteria (oxic-anoxic interface) and Bacteroidetes (upper hypolimnion), over Archaea (1.0% – 4.5%; maximum abundances at the oxic-anoxic interface). In turn, flow cytometry evidenced the dominance of HNA cells in the euphotic layer, whereas the proportion of LNA cells increased with depth. HNA and LNA populations were still observed in the anoxic hypolimnion suggesting facultative or strict anaerobic metabolisms. The detection of distinct depth maxima of nitrate, nitrite, archaeal amoA and Marine Thaumarchaeota 16S gene copy numbers together with regularly detection of deep maxima of 3H-Thymidine uptake, and the presence of low-light adapted GSB species point towards a strong link of N, C, and S cycles in the redoxcline of Lake Kivu. [less ▲]

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See detailGalectin-9 splice variants in endothelial cells: identification of novel variants and functional characterization
Heusschen, Roy ULg; De Bree, Martijn; Griffioen, Arjan et al

in Angiogenesis (2011)

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See detailOptimal and robust event-related designs for fMRI
Maus, Bärbel ULg; van Breukelen, Gerard J P; Goebel, R et al

Conference (2011)

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See detailClosure to "Energy Dissipation and Turbulent Production in Weak Hydraulic Jumps" by E. Mignot and R. Cienfuegos
Mignot, E.; Peltier, Yann ULg; Cienfuegos, R.

in Journal of Hydraulic Engineering (2011), 137(8), 862-863

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See detailLinearly polarized second harmonic generation microscopy reveals chirality (vol 18, pg 8286, 2010)
Valev, V. K.; Silhanek, Alejandro ULg; Smisdom, N. et al

in Optics Express (2011), 19(10), 9242-9244

An erratum is presented to clarify both the direction of light polarization in our experiments and the unit cell geometry of the samples. Additionally, the spectral dependence of the second harmonic ... [more ▼]

An erratum is presented to clarify both the direction of light polarization in our experiments and the unit cell geometry of the samples. Additionally, the spectral dependence of the second harmonic generation response in the G-shaped nanostructures made of gold is corrected. These changes do not affect our previous conclusions. (C) 2011 Optical Society of America [less ▲]

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See detailA partial hybrid stress solid-shell element for the analysis of laminated composites
Rah, Kamran; Van Paepegem, Wim; Habraken, Anne ULg et al

in Computer Methods in Applied Mechanics and Engineerinrg (2011), 200(49-52), 35263539

In this paper a low order partial hybrid stress solid-shell element based on the composite energy functional for the analysis of laminated composite structures is presented. This solid-shell element has ... [more ▼]

In this paper a low order partial hybrid stress solid-shell element based on the composite energy functional for the analysis of laminated composite structures is presented. This solid-shell element has eight nodes with only displacement degrees of freedoms, and three-dimensional constitutive models can be directly employed in the present formulation without any additional treatment. The assumed interlaminar stress field provides very accurate interlaminar stress calculation through the element thickness. These elements can be stacked on top of each other to model multilayer structures, fulfilling the interlaminar stress continuity at the interlayer surfaces and zero traction conditions on the top and bottom surfaces of the laminate. The present solid-shell does not show the transverse shear, trapezoidal and thickness locking phenomenon. [less ▲]

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See detailFrom shallow water to deep mound - sedimentology and stromatoporoids paleoecology from the Frasnian (Upper Devonian) of Belgium
Da Silva, Anne-Christine ULg; Kershaw, Stephen; Boulvain, Frédéric ULg

in Kölner Forum für Geologie und Paläontologie (2011), 19

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See detailEn guise de conclusion : chronologie, technologies et faciès culturels du Gravettien français
Noiret, Pierre ULg

in Pesesse, Damien; Goutas, Nejma; Klaric, Laurent (Eds.) et al À la recherche des identités gravettiennes : actualités, questionnements et perspectives (2011)

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See detailCoping with Minority Status: Responses to Exclusion and Inclusion
Martiniello, Marco ULg

in Contemporary Sociology: a Journal of Reviews (2011), 40(2), 158-159

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See detailNotched Specimens Fracture Prediction with an Advanced GTN Model
Fansi Tchonko, Joseph ULg; Ben Bettaieb, Mohamed ULg; Balan, Tudor et al

in Key Engineering Materials [=KEM] (2011), 488-489

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See detailLa tradizione fiorentina della Supplicatione d’Italia al re di Francia. Con una nuova edizione critica
Moreno, Paola ULg

in Filologia e Critica (2011), XXXV(2), 1-33

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See detailLe Michelsberg de Pont-de-Bonne (province de Liège, Belgique)
Delye, Emmanuel ULg; Gilson, Simon-Pierre; Noiret, Pierre ULg

in Revue Archéologique de Picardie (2011)

Since the beginning of research on the éperon barré of Pont-de-Bonne (Modave, Belgium), we have recovered artefacts attributed to the Middle Neolithic. More recently, during excavation of the entrance ... [more ▼]

Since the beginning of research on the éperon barré of Pont-de-Bonne (Modave, Belgium), we have recovered artefacts attributed to the Middle Neolithic. More recently, during excavation of the entrance system of the fortification, a pot hole and a waste accumulation zone have been analysed. These structures contained abundant archaeological material (ceramic sherd, lithic and bone artefact and fauna). Three radiometric dates have been obtained. The ceramic assemblage is typical of the Belgian Michelsberg and a decorated vase shows Bischheim affinities. [less ▲]

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See detailDiscovery of English Grain Aphid (Hemiptera: Aphididae) Biotypes in China
Xu, Zhao-Huan; Chen, Julian; Cheng, Deng-Fa et al

in Journal of Economic Entomology (2011), 104(3), 1080-1086

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See detailSafety and Performance of Stochastic Targeted (STAR) Glycemic Control of Insulin and Nutrition – First Pilot Results
Shaw, Geoffrey M.; Le Compte, Aaron; Evans, Alicia et al

in Intensive Care Medicine (2011)

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See detailCombining intercropping with semiochemical releases: optimization of alternative control of Sitobion avenae 1 in wheat crops in China
Wang, Guang; Cui, Liang-Liang; Dong, Jie et al

in Entomologia Experimentalis et Applicata (2011)

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See detailBrain functional integration decreases during propofol-induced loss of consciousness.
Schrouff, Jessica ULg; Perlbarg, Vincent; Boly, Mélanie ULg et al

in NeuroImage (2011), 57(1), 198-205

Consciousness has been related to the amount of integrated information that the brain is able to generate. In this paper, we tested the hypothesis that the loss of consciousness caused by propofol ... [more ▼]

Consciousness has been related to the amount of integrated information that the brain is able to generate. In this paper, we tested the hypothesis that the loss of consciousness caused by propofol anesthesia is associated with a significant reduction in the capacity of the brain to integrate information. To assess the functional structure of the whole brain, functional integration and partial correlations were computed from fMRI data acquired from 18 healthy volunteers during resting wakefulness and propofol-induced deep sedation. Total integration was significantly reduced from wakefulness to deep sedation in the whole brain as well as within and between its constituent networks (or systems). Integration was systematically reduced within each system (i.e., brain or networks), as well as between networks. However, the ventral attentional network maintained interactions with most other networks during deep sedation. Partial correlations further suggested that functional connectivity was particularly affected between parietal areas and frontal or temporal regions during deep sedation. Our findings suggest that the breakdown in brain integration is the neural correlate of the loss of consciousness induced by propofol. They stress the important role played by parietal and frontal areas in the generation of consciousness. [less ▲]

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See detailSafety and Performance of Stochastic Targeted (STAR) Glycemic Control of Insulin and Nutrition - First Pilot Results
Shaw, Geoffrey M.; Le Compte, Aaron; Evans, Alicia et al

Poster (2011)

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See detailCharacterization of the response of GFP microbial biosensors sensitive to substrate limitation in scale-down bioreactors
Delvigne, Frank ULg; Brognaux, Alison ULg; Gorret, Nathalie et al

in Biochemical Engineering Journal (2011), 55(2), 131-139

The dynamics of microbial stress response in intensive cultivation conditions remains not completely understood. In this work, two green fluorescent protein (GFP) transcriptional reporters have been used ... [more ▼]

The dynamics of microbial stress response in intensive cultivation conditions remains not completely understood. In this work, two green fluorescent protein (GFP) transcriptional reporters have been used as biosensors of the heterogeneities generated in a two-compartment scale-down reactor. The stress promoters have been chosen for their responsiveness to carbon limitation corresponding to the global substrate profiles encountered in intensive fed-batch cultures. From our results, it can be concluded that the exposure of microbial cells to substrates heterogeneities tends to decrease the GFP expression level in fed-batch mode. Fluorescence intensities have been monitored at the single cell level by using flow cytometry. During the course of the fed-batch culture, a drop at the level of the intracellular GFP content has been observed for the two scale-down operating conditions and for the two promoters sensitive to substrate limitation (rpoS and csiE). The fluorescence drop can be attributed to the repression of these promoters but also to the release of GFP to the extracellular medium according to the increase of the fluorescence level of the supernatant. This leakage has been observed for all the operating conditions, i.e. the scale-down reactors and the culture operating in the normal mode, i.e. in a well-mixed bioreactor. Interestingly, GFP leakage is more pronounced in the case of the cultures operated in the normal mode. Indeed, staining by propidium iodide tends to be more elevated for the microbial cells cultured under the normal mode by comparison with those cultured in scale-down conditions, indicating a higher permeability of the membrane. These results suggest that GFP microbial biosensors could be used to detect simultaneously mixing imperfections and their impact on the viability of microorganisms. [less ▲]

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See detailInositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells
Marechal, Y.; Queant, S.; Polizzi, S. et al

in Immunobiology (2011), 216

Inositol 1,4,5-trisphosphate 3-kinase B (or Itpkb) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), its reaction product, play an important role in the control of B lymphocyte fate and function in ... [more ▼]

Inositol 1,4,5-trisphosphate 3-kinase B (or Itpkb) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), its reaction product, play an important role in the control of B lymphocyte fate and function in vivo. In order to investigate the fine mechanisms of Itpkb and Ins(1,3,4,5)P4 action in B cells, we crossed Itpkb(-/-) mice with transgenic mice expressing a 3-83mudelta B cell receptor (BCR) specific for membrane-bound MHC-I H2-K(b) and H2-K(k) molecules. On a non-deleting H2-K(d) genetic background, we show that Itpkb is important for the control of Bim protein expression and B cell survival rather than for the control of B cell development from one stage to another. Analyses of cell surface markers expression, proapoptotic Bim protein expression, in vitro survival and in vivo turnover demonstrated that BCR transgenic Itpkb(-/-) B cells exhibit an anergic phenotype with the notable exception of their enhanced antigen-induced calcium signalling. On a deleting H2-K(b) genetic background, we show that Itpkb is not essential for BCR editing or negative selection. These data establish Itpkb as an important regulator of B cell survival and anergy in vivo. [less ▲]

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