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See detailMicrobial Diversity and Processes in Lake Kivu (East Africa)
Llirós, M.; Darchambeau, François ULg; Garcia-Armisen, T. et al

Conference (2011)

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3 ... [more ▼]

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3, respectively) in the deep anoxic monimolimnion (from 60 o 480 m depth). Although there are a wealth of information on the ecology of small eukaryotes and their trophic role on Kivu, available information on prokaryotic planktonic assemblages is scarce. Molecular analysis of archaeal and bacterial communities showed a vertical segregation imposed by the permanent redoxcline. In relation to Bacteria, Actinobacteria, Betaproteobacteria, Green Sulfur Bacteria and Bacteroidetes were the most commonly retrieved groups. For Archaea, a marked dominance of Thaumarchaeota and Crenarchaeota (75% of all archaeal OTUs) over Euryarchaeota was observed. In the anoxic hypolimnion, Euryarchaoeta (Methanosarcinales and Methanocellales) lineages together with Miscellaneous Crenarchaeotic Group phylotypes were mainly recovered. In turn, Thaumarchaeota phylotypes were recovered in oxic and suboxic waters. CARDFISH analyses over the first 100 m revealed the dominance of Bacteria (51.4% – 95.7% of DAPI-stained cells), especially Actinobacteria (epilimnion), Betaproteobacteria (oxic-anoxic interface) and Bacteroidetes (upper hypolimnion), over Archaea (1.0% – 4.5%; maximum abundances at the oxic-anoxic interface). In turn, flow cytometry evidenced the dominance of HNA cells in the euphotic layer, whereas the proportion of LNA cells increased with depth. HNA and LNA populations were still observed in the anoxic hypolimnion suggesting facultative or strict anaerobic metabolisms. The detection of distinct depth maxima of nitrate, nitrite, archaeal amoA and Marine Thaumarchaeota 16S gene copy numbers together with regularly detection of deep maxima of 3H-Thymidine uptake, and the presence of low-light adapted GSB species point towards a strong link of N, C, and S cycles in the redoxcline of Lake Kivu. [less ▲]

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See detailSimulations numériques de figures de diffractions à l'usage de la sixième secondaire et du premier baccalauréat
Leblanc, Christophe ULg

in Bulletin de l'Association Belge des Professeurs de Physique et de Chimie [Bulletins de l'ABPPC] (2011), 49(190),

La diffraction constitue de nos jours un phénomène physique incontournable pour la recherche (diffraction par rayons X, diffraction de neutrons, cristallographie, ...) et est, à juste titre, enseignée ... [more ▼]

La diffraction constitue de nos jours un phénomène physique incontournable pour la recherche (diffraction par rayons X, diffraction de neutrons, cristallographie, ...) et est, à juste titre, enseignée aussi bien en dernière année de secondaire qu'à l'université. Le logiciel présenté ci-dessous est un programme permettant de réaliser des figures de diffractions de manière simple et intuitive. Il existe naturellement déjà de nombreux programmes de simulations de figures de diffractions que l'on peut facilement trouver sur internet. Cependant ceux-ci sont, pour la plupart, ou bien limités (à la connaissance de l'auteur) à quelques cas particuliers - diffractions sur une ou plusieurs fentes, un rond, plus rarement un carré - ou bien complexes à l'utilisation. Bien que ces programmes soient dignes d'intérêts et peuvent se montrer utiles dans le cadre d'un enseignement, leurs limitations empêchent d'acquérir une intuition réelle de ce qu'est la diffraction. Par exemple, quelle est la figure de diffraction obtenue par un réseau cristallin cubique, un réseau hexagonal, ou encore une hélice ? C'est précisément cette faille pédagogique que vient combler le logiciel présenté ci-dessous. En effet, celui-ci permet d'obtenir la figure de diffraction de « n'importe quelle forme » (en deux dimensions), « forme » entièrement paramétrable par l'utilisateur au moyen d'un simple fichier bitmap ; format de fichier graphique le plus répandu et le plus simple à employer. Il est naturellement également possible d'effectuer de la diffraction de manière expérimentale au moyen d'un simple laser hélium-néon (facilement accessible dans le commerce et d'un prix modéré) et de diapositives. Cependant, outre que le logiciel présenté ci-dessous peut-être obtenu gratuitement par simple demande à l'auteur, celui-ci se montre beaucoup plus souple d'utilisation que de simples diapositives car entièrement paramétrable, et, comme tout logiciel, échappe aux aléas expérimentaux (poussières sur les diapositives, problèmes d'alignements diapositives-laser, nécessité d'un local sombre, ...) [less ▲]

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See detailBulletin de la Commission royale pour la publication des Anciennes Lois et Ordonnances de Belgique
Marchandisse, Alain ULg

Book published by Ministère de la Justice (2011)

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See detailFlow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
Wauters, Jella; Franck, Thierry ULg; Pille, Frederik et al

in Veterinary Immunology and Immunopathology (2011), 144(3-4), 417-22

Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However ... [more ▼]

Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively. [less ▲]

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See detailEnd of life care in the operating room for non-heart-beating donors: organization at the University Hospital of Liege.
JORIS, Jean ULg; KABA, Abdourahmane ULg; LAUWICK, Séverine ULg et al

in Transplantation Proceedings (2011), 43(9), 3441-4

Non-heart-beating (NHB) organ donation has become an alternative source to increase organ supply for transplantation. A NHB donation program was implemented in our institution in 2002. As in many ... [more ▼]

Non-heart-beating (NHB) organ donation has become an alternative source to increase organ supply for transplantation. A NHB donation program was implemented in our institution in 2002. As in many institutions the end of life care of the NHB donor (NHBD) is terminated in the operating room (OR) to reduce warm ischemia time. Herein we have described the organization of end of life care for these patients in our institution, including the problems addressed, the solution proposed, and the remaining issues. Emphasis is given to our protocol elaborated with the different contributors of the chain of the NHB donation program. This protocol specifies the information mandatory in the medical records, the end of life care procedure, the determination of death, and the issue of organ preservation measures before NHBD death. The persisting malaise associated with NHB donation reported by OR nurses is finally documented using an anonymous questionnaire. [less ▲]

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See detailDO PLACEBO BASED VALIDATION STANDARDS MIMIC REAL BATCH PRODUCTS BEHAVIOUR? CASE STUDIES
Bouabidi, A.; Talbi, M.; Bouklouze, A. et al

in Journal of Pharmaceutical & Biomedical Analysis (2011), 55

Analytical methods validation is a mandatory step to evaluate the ability of developed methods to provide accurate results for their routine application. Validation usually involves validation standards ... [more ▼]

Analytical methods validation is a mandatory step to evaluate the ability of developed methods to provide accurate results for their routine application. Validation usually involves validation standards or quality control samples that are prepared in placebo or reconstituted matrix made of a mixture of all the ingredients composing the drug product except the active substance or the analyte under investigation. However, one of the main concerns that can be made with this approach is that it may lack an important source of variability that come from the manufacturing process. The question that remains at the end of the validation step is about the transferability of the quantitative performance from validation standards to real authentic drug product samples. In this work, this topic is investigated through three case studies. Three analytical methods were validated using the commonly spiked placebo validation standards at several concentration levels as well as using samples coming from authentic batch samples (tablets and syrups). The results showed that, depending on the type of response function used as calibration curve, there were various degrees of differences in the results accuracy obtained with the two types of samples. Nonetheless the use of spiked placebo validation standards was showed to mimic relatively well the quantitative behaviour of the analytical methods with authentic batch samples. Adding these authentic batch samples into the validation design may help the analyst to select and confirm the most fit for purpose calibration curve and thus increase the accuracy and reliability of the results generated by the method in routine application. [less ▲]

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See detailScreening for colorectal cancer in asymptomatic average risk patients: role of imaging.
Hock, D.; Ouhadi, R.; Materne, R. et al

in Acta Gastro-Enterologica Belgica (2011), 74(1), 70-6

Early detection of colorectal cancer or advanced adenomas is a public health priority in many industrialized countries. There are various methods of screening average risk individuals for colorectal ... [more ▼]

Early detection of colorectal cancer or advanced adenomas is a public health priority in many industrialized countries. There are various methods of screening average risk individuals for colorectal cancer, and their effectiveness may depend on subjective parameters like local expertise and patient's preferences. This paper reviews these tests with special emphasis regarding imaging techniques that aim to provide less-invasive alternatives to optical colonoscopy (OC) which is the standard of reference. Both Double-Contrast Barium Enema (DCBE) and Virtual Colonoscopy (VC) have >90% sensitivity compared to OC in the detection of clinically relevant colonic lesions. Nevertheless, VC may have an edge over DCBE for technical and reproductivity reasons, as well as greater learning opportunities. Imaging techniques criticisms regarding diminutive and flat lesions, cost, radiation exposure and effects on gastroenterological practice are addressed. [less ▲]

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See detailNonlinear Normal Modes: A Finite Element Approach
Renson, Ludovic ULg; Kerschen, Gaëtan ULg

Conference (2011)

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See detailFrom shallow water to deep mound - sedimentology and stromatoporoids paleoecology from the Frasnian (Upper Devonian) of Belgium
Da Silva, Anne-Christine ULg; Kershaw, Stephen; Boulvain, Frédéric ULg

in Kölner Forum für Geologie und Paläontologie (2011), 19

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See detailAdalimumab produces clinical remission and reduces extraintestinal manifestations in Crohn's disease: Results from CARE.
Löfberg, Robert; Louis, Edouard ULg; Reinisch, W. et al

in Inflammatory Bowel Diseases (2011)

BACKGROUND: Data regarding the effectiveness of anti-tumor necrosis factor (TNF) agents for resolution of extraintestinal manifestations (EIMs) are scarce. The CARE study evaluated clinical effectiveness ... [more ▼]

BACKGROUND: Data regarding the effectiveness of anti-tumor necrosis factor (TNF) agents for resolution of extraintestinal manifestations (EIMs) are scarce. The CARE study evaluated clinical effectiveness, EIM resolution, and safety of adalimumab in a large pan-European cohort of patients with moderate to severe Crohn's disease (CD). METHODS: In all, 945 patients with a Harvey-Bradshaw Index (HBI) >/=7 enrolled in this multicenter, open-label phase IIIb trial. Patients received subcutaneous adalimumab, 160/80 mg at weeks 0/2, then 40 mg every other week. Dose adjustments were allowed for CD-related concomitant medications (from week 8) and adalimumab (from week 12). Clinical endpoints were analyzed through week 20 for all patients, and after stratification by prior infliximab exposure and by reason for discontinuing infliximab (primary nonresponse [PNR] or other). RESULTS: The remission rate (HBI <5) at week 20 was 52% (95% confidence interval, 49%-55%) overall, and was higher for infliximab-naive versus infliximab-exposed patients (62% versus 42%, P < 0.001). Remission rates were similar for PNR (37%) and other reasons (43%; P = 0.278). Of 497 patients with baseline EIMs, 51% were free of EIM signs and symptoms at week 20. Serious infectious adverse events were reported in 5% of patients. Opportunistic infections and malignancies were rare (</=1%). There was one case of demyelinating disease, but no occurrences of lupus, tuberculosis, or death. CONCLUSIONS: In this large cohort of patients, adalimumab treatment resulted in rates of clinical remission and EIM resolution exceeding 50%, and substantial rates of effectiveness in patients who had PNR to infliximab. Adalimumab was well tolerated, with safety consistent with prior reports. (Inflamm Bowel Dis 2011). [less ▲]

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See detailDeterminants of high, median and low rates of caesarean deliveries in Belgium
Absil, Gaëtan ULg; Van Parys, A. S.; Bednarek, Stéphanie et al

Report (2011)

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See detailDiscovery of English Grain Aphid (Hemiptera: Aphididae) Biotypes in China
Xu, Zhao-Huan; Chen, Julian; Cheng, Deng-Fa et al

in Journal of Economic Entomology (2011), 104(3), 1080-1086

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See detailIntroduction
Breux, Sandra; Reuchamps, Min ULg

in Breux, Sandra; Reuchamps, Min; Loiseau, Hugo (Eds.) Carte mentale et science politique : Regards et perspectives critiques sur l’emploi d’un outil prometteur (2011)

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See detailAuteurs juifs et chrétiens (Ier - IIIe siècles) : Introduction et bibliographie
Carlig, Nathan ULg

Textual, factual or bibliographical database (2011)

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See detailL’adaptation au changement climatique en région wallonne : Fiche thématique : Biodiversité
Frisson, Gwenn ULg; Monty, Arnaud ULg; Mahy, Grégory ULg et al

Report (2011)

• Les changements attendus sont importants mais difficiles à quantifier. Seules des tendances peuvent être dégagées. • La fragmentation des habitats naturels est particulièrement poussée en Wallonie. Elle ... [more ▼]

• Les changements attendus sont importants mais difficiles à quantifier. Seules des tendances peuvent être dégagées. • La fragmentation des habitats naturels est particulièrement poussée en Wallonie. Elle constitue une lourde menace sur la biodiversité et une entrave aux changements d’aires de distribution attendus. • Développer le réseau écologique et diminuer les menaces qui pèsent actuellement sur la biodiversité est primordial pour donner une chance à la biodiversité de s’adapter au changement climatique. • Les impacts de la perte de biodiversité ne doivent pas seulement se mesurer en termes de valeur intrinsèque mais également en termes de services écosystémiques. [less ▲]

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See detailCombining intercropping with semiochemical releases: optimization of alternative control of Sitobion avenae 1 in wheat crops in China
Wang, Guang; Cui, Liang-Liang; Dong, Jie et al

in Entomologia Experimentalis et Applicata (2011)

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See detailL'imperatrice. Le Molte Vite di Indira Gandhi
Maiorano, Diego ULg

in Storia della Storiografia (2011), 58

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