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See detailTrabectedine (ET-743/Yondelis) dans le traitement des sarcomes des tissus mous et du cancer de l'ovaire.
GENNIGENS, Christine ULg; Jerusalem, Guy ULg

in Revue Médicale de Liège (2011), 66(7-8), 452-5

Soft tissue sarcomas account for 1% of all malignant tumours. Until a few years ago, doxorubicine and ifosfamide were the only active chemotherapy drugs in the metastatic setting. Recently, a new drug has ... [more ▼]

Soft tissue sarcomas account for 1% of all malignant tumours. Until a few years ago, doxorubicine and ifosfamide were the only active chemotherapy drugs in the metastatic setting. Recently, a new drug has proven its efficacy after failure of standard treatments: the trabectedin; its activity is based on interference with ADN repair mechanisms. Trabectedin has just been also validated and reimbursed in patients with ovarian cancer, in partially sensitive recurrence. In this paper, we will review the mechanism of action and the clinical results of trabectedin. [less ▲]

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See detailExpression, purification of novel alkaline Staphylococcus xylosus lipase acting at high temperature
Bouaziz, Ahlem ULg; Habib, Horchani; Ben Salem, Nadia et al

in Biochemical Engineering Journal (2011), 54

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See detailMicro-CT-based improvement of geometrical and mechanical controllability of selective laser melted Ti6Al4V porous structures
Van Bael, S.; Kerckhofs, Greet ULg; Moesen, M. et al

in MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING (2011), 528(24), 7423-7431

Despite the fact that additive manufacturing (AM) techniques allow to manufacture complex porous parts with a controlled architecture, differences can occur between designed and as-produced morphological ... [more ▼]

Despite the fact that additive manufacturing (AM) techniques allow to manufacture complex porous parts with a controlled architecture, differences can occur between designed and as-produced morphological properties. Therefore this study aimed at optimizing the robustness and controllability of the production of porous Ti6Al4V structures using selective laser melting (SLM) by reducing the mismatch between designed and as-produced morphological and mechanical properties in two runs. In the first run, porous Ti6Al4V structures with different pore sizes were designed, manufactured by SLM, analyzed by microfocus X-ray computed tomography (micro-CT) image analysis and compared to the original design. The comparison was based on the following morphological parameters: pore size, strut thickness, porosity, surface area and structure volume. Integration of the mismatch between designed and measured properties into a second run enabled a decrease of the mismatch. For example, for the average pore size the mismatch decreased from 45% to 5%. The demonstrated protocol is furthermore applicable to other 3D structures, properties and production techniques, powder metallurgy, titanium alloys, porous materials, mechanical characterization, tomography. (C) 2011 Elsevier B.V. All rights reserved. [less ▲]

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See detailAnalytical validation of the Liaison Calcitonin_II-Gen (DiaSorin)
Cavalier, Etienne ULg; Carlisi, Ignazia ULg; Bekaert, Anne-Catherine ULg et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 271-275

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See detailLe centre, les planètes et la noirceur : Controverses cosmiques et terrestres
Nazé, Yaël ULg

Article for general public (2011)

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See detailControl of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii.
Tolleter, Dimitri; Ghysels, Bart ULg; Alric, Jean et al

in Plant Cell (2011), 23(7), 2619-30

Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H(2) production is a transitory ... [more ▼]

Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H(2) production is a transitory phenomenon under most natural conditions, often viewed as a safety valve protecting the photosynthetic electron transport chain from overreduction. From the colony screening of an insertion mutant library of the unicellular green alga Chlamydomonas reinhardtii based on the analysis of dark-light chlorophyll fluorescence transients, we isolated a mutant impaired in cyclic electron flow around photosystem I (CEF) due to a defect in the Proton Gradient Regulation Like1 (PGRL1) protein. Under aerobiosis, nonphotochemical quenching of fluorescence (NPQ) is strongly decreased in pgrl1. Under anaerobiosis, H(2) photoproduction is strongly enhanced in the pgrl1 mutant, both during short-term and long-term measurements (in conditions of sulfur deprivation). Based on the light dependence of NPQ and hydrogen production, as well as on the enhanced hydrogen production observed in the wild-type strain in the presence of the uncoupling agent carbonyl cyanide p-trifluoromethoxyphenylhydrazone, we conclude that the proton gradient generated by CEF provokes a strong inhibition of electron supply to the hydrogenase in the wild-type strain, which is released in the pgrl1 mutant. Regulation of the trans-thylakoidal proton gradient by monitoring pgrl1 expression opens new perspectives toward reprogramming the cellular metabolism of microalgae for enhanced H(2) production. [less ▲]

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See detailValdeblore (06) Le Clouté
Pagès, Gaspard ULg

in D.R.A.C., S.R.A. (Ed.) Bilan scientifique de la région Provence-Alpes-Côte d’Azur 2010 (2011)

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See detailOrganisation du territoire de la cité antique de Narbonne : contraintes environnementales et exploitations des ressources
Cavero, Caroline; Sanchez, Corinne; Jézégou, Marie-Pierre et al

in Cavero, Caroline; Sanchez, Corinne; Jézégou, Maries-Pierre (Eds.) et al Fréjus romaine, la ville et son territoire : agglomérations de Narbonnaise, des Alpes-Maritimes et de Cisalpine à travers la recherche archéologique : actes du 8e Colloque historique de Fréjus, 8-10 octobre 2010 (2011)

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See detailSupraorbital transcutaneous neurostimulation has sedative effects in healthy subjects.
Piquet, Maxime; Balestra, Costantino; SAVA, Simona Liliana ULg et al

in BMC Neurology (2011), 11

BACKGROUND: Transcutaneous neurostimulation (TNS) at extracephalic sites is a well known treatment of pain. Thanks to recent technical progress, the Cefaly(R) device now also allows supraorbital TNS ... [more ▼]

BACKGROUND: Transcutaneous neurostimulation (TNS) at extracephalic sites is a well known treatment of pain. Thanks to recent technical progress, the Cefaly(R) device now also allows supraorbital TNS. During observational clinical studies, several patients reported decreased vigilance or even sleepiness during a session of supraorbital TNS. We decided therefore to explore in more detail the potential sedative effect of supraorbital TNS, using standardized psychophysical tests in healthy volunteers. METHODS: We performed a double-blind cross-over sham-controlled study on 30 healthy subjects. They underwent a series of 4 vigilance tests (Psychomotor Vigilance Task, Critical Flicker Fusion Frequency, Fatigue Visual Numeric Scale, d2 test). Each subject was tested under 4 different experimental conditions: without the neurostimulation device, with sham supraorbital TNS, with low frequency supraorbital TNS and with high frequency supraorbital TNS. RESULTS: As judged by the results of three tests (Psychomotor Vigilance Task, Critical Flicker Fusion Frequency, Fatigue Visual Numeric Scale) there was a statistically significant (p < 0.001) decrease in vigilance and attention during high frequency TNS, while there were no changes during the other experimental conditions. Similarly, performance on the d2 test was impaired during high frequency TNS, but this change was not statistically significant. CONCLUSION: Supraorbital high frequency TNS applied with the Cefaly(R) device decreases vigilance in healthy volunteers. Additional studies are needed to determine the duration of this effect, the underlying mechanisms and the possible relation with the stimulation parameters. Meanwhile, this effect opens interesting perspectives for the treatment of hyperarousal states and, possibly, insomnia. [less ▲]

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See detailField trip 1: Givetian and Frasnian of Southern Belgium
Boulvain, Frédéric ULg; Coen-Aubert, Marie; Da Silva, Anne-Christine ULg et al

in Kölner Forum für Geologie und Paläontologie (2011), 20

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See detailGait feature extraction in Parkinson's disease using low-cost accelerometers
Stamatakis, Julien; Cremers, Julien ULg; Maquet, Didier ULg et al

Poster (2011)

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling ... [more ▼]

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling, short steps, freezing of gait (FoG) and/or asymmetries in gait. There are currently no validated clinical instruments or device that allow a full characterization of gait disturbances in PD. As a step towards this goal, a four accelerometer-based system is proposed to increase the number of parameters that can be extracted to characterize parkinsonian gait disturbances such as FoG or gait asymmetries. After developing the hardware, an algorithm has been developed, that automatically epoched the signals on a stride-by-stride basis and quantified, among others, the gait velocity, the stride time,the stance and swing phases, the single and double support phases or the maximum acceleration at toe-off, as validated by visual inspection of video recordings during the task. The results obtained in a PD patient and an healthy volunteer are presented. The FoG detection will be improved using time-frequency analysis and the system is about to be validated with a state-of-the-art 3D movement analysis system. [less ▲]

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See detailAntibody production by injection of living cells expressing non self antigens as cell surface type II transmembrane fusion protein.
Nizet, Yannick; Gillet, Laurent ULg; SCHROEDER, Hélène ULg et al

in Journal of immunological methods (2011)

Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps ... [more ▼]

Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps. This method relies on the injection of histocompatible living cells stably expressing the antigen as a cell surface type II transmembrane fusion protein. A vector, nicknamed pCD1-CD134L, was constructed to express the antigen fused at the carboxyterminal end of the human CD134 ligand (CD134L) type II transmembrane protein on the surface of eucaryotic cells. This vector was shown to induce cell surface expression of epitopes from human c-Myc (soluble protein), uterogloblin-related protein 1 (secreted protein) and CD94 (type II transmembrane protein). Using this vector, we developed a method to produce antibodies without antigen production. The flowchart of this method is as follows: (i) cloning of the antigen in the pCD1-CD134L vector; (ii) production of a histocompatible cell line stably expressing the CD134L-antigen fusion protein; (iii) testing for cell surface expression of the fusion protein by targeting the CD134L carrier; and (iv) prime-boost immunisation with living cells expressing the fusion protein. This method was successfully used for production of polyclonal antibodies raised against Ixodes ricinus calreticulin (secreted protein) in mice and for production of monoclonal antibodies raised against an epitope of Vaccinia virus A56 (type I transmembrane protein) protein in rat. The present study is the first to demonstrate the use of a type II transmembrane protein as a carrier for cell surface display of antigens. [less ▲]

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See detailContinuous-flow production of alkyl nitrites
Monbaliu, Jean-Christophe ULg; Jorda, Jeremy; Chevalier, Bérengère et al

in Chimica Oggi = Chemistry Today (2011), 29

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See detailCamel Trekking for non motile bacteria
Rigali, Sébastien ULg

Conference (2011)

Detailed reference viewed: 13 (4 ULg)