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See detailMicrobial Diversity and Processes in Lake Kivu (East Africa)
Llirós, M.; Darchambeau, François ULg; Garcia-Armisen, T. et al

Conference (2011)

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3 ... [more ▼]

Lake Kivu is a deep meromictic and oligotrophic tropical African lake with a permanent thermal- and haline stratification with huge accumulations of dissolved CO2 and CH4 (ca. 300 km3 and 60 km3, respectively) in the deep anoxic monimolimnion (from 60 o 480 m depth). Although there are a wealth of information on the ecology of small eukaryotes and their trophic role on Kivu, available information on prokaryotic planktonic assemblages is scarce. Molecular analysis of archaeal and bacterial communities showed a vertical segregation imposed by the permanent redoxcline. In relation to Bacteria, Actinobacteria, Betaproteobacteria, Green Sulfur Bacteria and Bacteroidetes were the most commonly retrieved groups. For Archaea, a marked dominance of Thaumarchaeota and Crenarchaeota (75% of all archaeal OTUs) over Euryarchaeota was observed. In the anoxic hypolimnion, Euryarchaoeta (Methanosarcinales and Methanocellales) lineages together with Miscellaneous Crenarchaeotic Group phylotypes were mainly recovered. In turn, Thaumarchaeota phylotypes were recovered in oxic and suboxic waters. CARDFISH analyses over the first 100 m revealed the dominance of Bacteria (51.4% – 95.7% of DAPI-stained cells), especially Actinobacteria (epilimnion), Betaproteobacteria (oxic-anoxic interface) and Bacteroidetes (upper hypolimnion), over Archaea (1.0% – 4.5%; maximum abundances at the oxic-anoxic interface). In turn, flow cytometry evidenced the dominance of HNA cells in the euphotic layer, whereas the proportion of LNA cells increased with depth. HNA and LNA populations were still observed in the anoxic hypolimnion suggesting facultative or strict anaerobic metabolisms. The detection of distinct depth maxima of nitrate, nitrite, archaeal amoA and Marine Thaumarchaeota 16S gene copy numbers together with regularly detection of deep maxima of 3H-Thymidine uptake, and the presence of low-light adapted GSB species point towards a strong link of N, C, and S cycles in the redoxcline of Lake Kivu. [less ▲]

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See detailTraumatologie du basketball
Kaux, Jean-François ULg; Crielaard, Jean-Michel ULg

Learning material (2011)

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See detailCompetitive facility location problem with attractiveness adjustment of the follower: A bilevel programming model and its solution
Kucukaydin, Hande ULg; Aras, Necati; Altinel, I. Kuban

in European Journal of Operational Research (2011), 208(3), 206-220

We are concerned with a problem in which a firm or franchise enters a market by locating new facilities where there are existing facilities belonging to a competitor. The firm aims at finding the location ... [more ▼]

We are concerned with a problem in which a firm or franchise enters a market by locating new facilities where there are existing facilities belonging to a competitor. The firm aims at finding the location and attractiveness of each facility to be opened so as to maximize its profit. The competitor, on the other hand, can react by adjusting the attractiveness of its existing facilities with the objective of maximizing its own profit. The demand is assumed to be aggregated at certain points in the plane and the facilities of the firm can be located at predetermined candidate sites. We employ Huff's gravity-based rule in modeling the behavior of the customers where the fraction of customers at a demand point that visit a certain facility is proportional to the facility attractiveness and inversely proportional to the distance between the facility site and demand point. We formulate a bilevel mixed-integer nonlinear programming model where the firm entering the market is the leader and the competitor is the follower. In order to find the optimal solution of this model, we convert it into an equivalent one-level mixed-integer nonlinear program so that it can be solved by global optimization methods. Apart from reporting computational results obtained on a set of randomly generated instances, we also compute the benefit the leader firm derives from anticipating the competitor's reaction of adjusting the attractiveness levels of its facilities. The results on the test instances indicate that the benefit is 58.33% on the average. [less ▲]

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See detailCoordination of verbal dependents in Old French : Coordination as a specified juxtaposition or apposition
Mazziotta, Nicolas ULg

in Gerdes, Kim; Hajičova, Eva; Wanner, Leo (Eds.) Proceedings of Depling 2011, International Conference on Dependency Linguistics, Barcelona (2011)

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See detail1D numerical modeling of the flow over a Piano Key Weir
Erpicum, Sébastien ULg; Machiels, Olivier ULg; Archambeau, Pierre ULg et al

in Erpicum, Sébastien; Laugier, Frédéric; Boillat, Jean Louis (Eds.) et al Labyrinth and Piano Key Weirs - PKW 2011 (2011)

Because of the PK-Weir geometric specificities, its hydraulic capacity remains difficult to predict without using experimental techniques. A 1D numerical model of the flow over a PK-Weir has been ... [more ▼]

Because of the PK-Weir geometric specificities, its hydraulic capacity remains difficult to predict without using experimental techniques. A 1D numerical model of the flow over a PK-Weir has been developed at the Research unit HACH at the University of Liege. It is based on a 1D modeling of the inlet and the outlet separately, with a single upstream reservoir and interactions between both flows by exchange of mass and momentum along the lateral crest. The comparison of the numerical results with various experimental data showed the ability of the numerical model to predict with reasonable accuracy the release capacity of a PK-Weir, whatever its geometry. The tests of the solver enable to highlight the significant influence of the inlet and outlet width on the weir release capacity. They also suggest that improvements of the numerical model may lie in the evaluation of the discharge coefficient of the lateral crest. [less ▲]

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See detailZinc complexes with 1,2,4-triazole functionalized amino acid derivatives: Synthesis, structure and b-lactamase assay
Naik, Anil; Beck, Joséphine; Dîrtu, Marinela et al

in Inorganica Chimica Acta (2011), 368

Coordinating abilities of 4R-1,2,4-triazole derivatives (R = glycine ethyl ester (L1), glycine (L2), diethylamino malonate (L3), methionine (L4) and diethyl aminomethylphosphonate (L5)) towards ZnII ions ... [more ▼]

Coordinating abilities of 4R-1,2,4-triazole derivatives (R = glycine ethyl ester (L1), glycine (L2), diethylamino malonate (L3), methionine (L4) and diethyl aminomethylphosphonate (L5)) towards ZnII ions have been studied in solution, in solid state and versus three zinc-b-lactamases. The crystal structure of [Zn3(L4)6(H2O)6] (6) is described; it is the first crystal structure involving a 1,2,4-triazole functionalized methionine. It forms a trinuclear complex with central zinc octahedrally coordinated by only L4, whereas terminal zinc ions coordination sphere is completed by three water molecules. L4 exhibits a dual functionality of a bridging bidentate ligand as well as an anion. A dense hydrogen bonding network connects these trinuclear entity into a 3D supramolecular network. The ZnII ions in 6 are held at equidistance (3.848 Å) which coincidently matches with the corresponding Zn Zn distance in the binuclear zinc enzyme from Bacillus cereus (3.848 and 4.365 Å). Among L1–L5 screened for b-lactamase assay, L4 shows modest inhibition for BcII enzyme. [less ▲]

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See detailMaîtrise de la situation d’enseignement-apprentissage et savoirs professionnels des futurs enseignants
Beckers, Jacqueline ULg

in Maubant, Philippe; Martineau, Stéphane (Eds.) Fondements des pratiques professionnelles des enseignants (2011)

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See detailOptimal linear taxation under endogenous longevity
Pestieau, Pierre ULg; Leroux, ML; Ponthiere, Gregory

in Journal of Population Economics (2011), 24

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See detailAntibody production by injection of living cells expressing non self antigens as cell surface type II transmembrane fusion protein.
Nizet, Yannick; Gillet, Laurent ULg; SCHROEDER, Hélène ULg et al

in Journal of immunological methods (2011)

Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps ... [more ▼]

Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps. This method relies on the injection of histocompatible living cells stably expressing the antigen as a cell surface type II transmembrane fusion protein. A vector, nicknamed pCD1-CD134L, was constructed to express the antigen fused at the carboxyterminal end of the human CD134 ligand (CD134L) type II transmembrane protein on the surface of eucaryotic cells. This vector was shown to induce cell surface expression of epitopes from human c-Myc (soluble protein), uterogloblin-related protein 1 (secreted protein) and CD94 (type II transmembrane protein). Using this vector, we developed a method to produce antibodies without antigen production. The flowchart of this method is as follows: (i) cloning of the antigen in the pCD1-CD134L vector; (ii) production of a histocompatible cell line stably expressing the CD134L-antigen fusion protein; (iii) testing for cell surface expression of the fusion protein by targeting the CD134L carrier; and (iv) prime-boost immunisation with living cells expressing the fusion protein. This method was successfully used for production of polyclonal antibodies raised against Ixodes ricinus calreticulin (secreted protein) in mice and for production of monoclonal antibodies raised against an epitope of Vaccinia virus A56 (type I transmembrane protein) protein in rat. The present study is the first to demonstrate the use of a type II transmembrane protein as a carrier for cell surface display of antigens. [less ▲]

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See detailChlamydomonas can play a role in the study of a heteroplasmic human mitochondrial mutation
Larosa, Véronique ULg; Coosemans, Nadine ULg; Bonnefoy, Nathalie et al

Scientific conference (2011)

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See detailPresent day carbon dioxide fluxes in the coastal ocean and possible feedbacks under global change
Borges, Alberto ULg

in da Silva Duarte, P. M.; Santana Casiano, J. M. (Eds.) Oceans and the atmospheric carbon content (2011)

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See detailSentiment d’altérité dans la correspondance de Rousseau en 1762
Biquet, Stéphanie ULg

in Turekova, Andrea (Ed.) Conférence internationale Autre - Autrui - Altérité (2011)

Jean-Jacques Rousseau est né avec la conscience de son altérité : fils d’horloger, citoyen de Genève, il se sent dès son plus jeune âge différent du monde qui l’entoure. Par sa « réforme » au début des ... [more ▼]

Jean-Jacques Rousseau est né avec la conscience de son altérité : fils d’horloger, citoyen de Genève, il se sent dès son plus jeune âge différent du monde qui l’entoure. Par sa « réforme » au début des années 1750, il transformera officiellement cette altérité en faire-valoir. Pourtant, quand résonne le glas de la condamnation de ses œuvres (Émile et le Contrat social) en 1762, l’homme jusque-là différent, se sent étranger, incompris, seul contre tous, comme en témoigne sa correspondance. Cet article se donne pour objectif d’étudier ce sentiment d’altérité et d’étrangeté à travers la correspondance personnelle de Jean-Jacques Rousseau de 1762 à 1765 (soit de la condamnation de l’Émile et du Contrat social jusqu'au départ pour l’Angleterre chez David Hume). [less ▲]

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See detailFrom shallow water to deep mound - sedimentology and stromatoporoids paleoecology from the Frasnian (Upper Devonian) of Belgium
Da Silva, Anne-Christine ULg; Kershaw, Stephen; Boulvain, Frédéric ULg

in Kölner Forum für Geologie und Paläontologie (2011), 19

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See detailLe modèle de croissance katangais face à la crise financière mondiale : enjeux en termes d'emploi
Lapeyre, Frédéric; Lebailly, Philippe ULg; Musewa M'Bayo, Laki et al

Book published by Bureau International du Travail (2011)

La crise financière mondiale qui a éclaté en 2008 et les effets désastreux qu’elle a occasionnés sur le secteur minier en RDC (qui avait été placé au centre des politiques de croissance et de lutte contre ... [more ▼]

La crise financière mondiale qui a éclaté en 2008 et les effets désastreux qu’elle a occasionnés sur le secteur minier en RDC (qui avait été placé au centre des politiques de croissance et de lutte contre la pauvreté) posent avec encore plus d’acuité la question de la diversification de la structure économique dans une province comme le Katanga. Au problème de la vulnérabilité d’un tel modèle de croissance s’ajoute le constat qui est fait dans cette étude du manque d’effet d’entrainement de l’activité des grands groupes miniers sur le reste de l’économie locale, en termes de développement des capacités productives et de création d’emplois. [less ▲]

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See detailDiscovery of English Grain Aphid (Hemiptera: Aphididae) Biotypes in China
Xu, Zhao-Huan; Chen, Julian; Cheng, Deng-Fa et al

in Journal of Economic Entomology (2011), 104(3), 1080-1086

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See detailMuscular forces affecting the outcome of CCL surgery.
Ramirez, L.; Böhme, Béatrice ULg; Vroomen, Carl ULg et al

in Proceedings of the 20th ECVS Congress (2011)

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See detailFinal results of NKTR-102, a topoisomerase I inhibitor-polymer conjugate, in patients (Pts) with pretreated metastatic breast cancer (MBC) demonstrating significant antitumor activity
Garcia, A; Awada, A; Chan, S et al

in Journal of Clinical Oncology (2011), 29(supplement 27),

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See detailCombining intercropping with semiochemical releases: optimization of alternative control of Sitobion avenae 1 in wheat crops in China
Wang, Guang; Cui, Liang-Liang; Dong, Jie et al

in Entomologia Experimentalis et Applicata (2011)

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